SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene229

Length: 2789 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene229
ATGGGGGGGATTTCATTTACGCTATCCACTGCAACACCCCGCTACCGTGTCCTGTAGAAATAAAATAAAAATATACTCAATTGGAATGGGGACTTCGAAGTGAGTGAGTGGTGCACGGCCATGGCACTCTCCGCCTCGGCCACGGCCTCATATACCAGGGGAAGAAGATTAGCCTTCAAAGAAACTTCATGCCTATCATCATTTAAAGGATCAGGATTTCTTTTTAACAAATGCTGGTTCCCACAGACGCGCTTTCCTCGGCGACTCGGGTTTGCTCGTTGTAATACAAGCAATGGAACAGCTCTTTCTGCCCCCGACTTTGAGAAAACGAGTATGGGCGCCAATATTTTCCTCTCGCCGCAGTTGTGGGACAGGATGCCATCAAGACAGCATTACTACTTGGAGCTGTTGATAATGCCATTGGTGGCATTGCCATTTCAGGGAAAAGAGGGACTGCCAAGACAGTAATGGCACGTGGATTGCATGCTATAATTCCACCTATTGAAGTTGTTGCGGGGTCTATTGCCAACTCTGACCCATTGTGTCCCGAAGAATGGGAAGAAGGACTAGAGAACCGACTAAAGTATAATGCTAATGGTACATTGAAAACAGATATAATAAAATCTCCATTTGTGCAGATACCACTTGGTGTAACAGAGGATAGGCTTTTAGGTTCAGTGGATGTAGAGGAGTCAGTCAAATCTGGAACAACGGTATTCCAGCCAGGTCTTCTTGCTGATGCTCACAGAGGAGTCCTATATGTTGATGAGATAAATCTTCTTGATGAAGGTATAAGCAATTTGCTTCTAAATGTTCTCACAGAAGGAGTTAATGTTGTGGAAAGAGAAGGAATCAGTTTTCGACATCCCTGCAAGCCACTTCTAATTGCTACGTACAATCCCGAGGAAGGTTCTGTACGAGAACATTTACTTGATCGCATTGCAATCAACCTCAGTGCTGATGTTCCCATGGAATTTGATGACCGTGTATCAGCAGTTGATCTTGCAATCCAATTCCAGGAAAAGTCCAAGCAAGTATTTCGGTCAGTTGAAGAAGGAACTGATTCAGCAAAGACACAGATCATTTTTGCAAGGGAATACCTAAAGGAAGTGAGCATTAGTAGGGATCAAATAAAGTATCTTGTTATGGAAGCCTTACGTGGTGGTTGTCAGGGTCACAGAGCTGAACTGTATGCTTCTAAAGTTGCAAGATCATTGGCCGCTCTTGAGGGTCGTGATAAAGTTGGGGTTGATGATCTCAAGAAAGCAGTAGAGCTGGTGATAATACCTCGTTCCACAGTTAATGAAAATCCACCGGAAAAACAGAACCAGCCTCCTCCACCGCCACCACCACCACCACAAAATCAGGATCCTGCAGAAGAACAAAACGAAGAAGATAAAGATGAGGAGGATGATGGTGATAATGAACAGCAAGAAGAAGAGCAAATACCAGATGAGTTTATCTTTGATGCAGAAGGTGGTTTGGTGGACTCAAAGCTGCTCTTCTTTGCACAACAGGCACAGAGGCGAAAGGGCAAAGCTGGTCGTGCCAAAAATGTAATATTTTCCGAGGATAGAGGACGATACATCAAGCCCATGCTTCCAAAGGGACCCATACGGAGGCTAGCCATTGATGCAACCCTAAGAGCAGCAGCACCCTATCAAAAATTGCGAAGACAGAGAGCTTTTGAAAAGGGAGAAAACAGAGGGTTTTTGTGGAGAAGACTGATATGAGAGCCAAGCGAATGGCCCGCAAAGCGGGAGCTCTGGTGATATTTGTTGTTGATGCCAGTGGAAGTATGGCACTGAATCGCATGCAAAATGCAAAAGGTGCTGCCATGCAACTTCTTTCTGAGAGCTATACAAGCAGAGATCAGGTATCAATTATTCCATTTCGTGGAGATGCAGCAGATGTCCTCCTACCACCATCTCGATCAATTGCAATGGCAAGAAAACGACTAGAGAGGCTACCTTGTGGTGGTGGCTCTCCCTTGGCTCATGGTCTTAGTATGGCTGTTCGAGTAGGATTAAATGCTGAGAAGAGTGGAGATGTTGGACGTGTGATGATAGTGGCTATCACAGATGGAAGGGCTAACATATCATTGAAAAGATCTACTGATCCAGATTCTAGTGCTACCGATTCACCAAAACCTTCCACACAAGAGTTGAAGGACGAGATATTAGAAATAGCAGGAAAGATTTACAAGGCAGGAATGTCCCTTCTTGTGATCGACACAGAGAATAAGTTTGTATCCACAGGGTTTGCAAAGGAGATTGCTAGAGTTGCTCAGGGGAAGTATTACTATTTACCTAATGCTTCCGATGCAGTTATATCCTCTGCAACAAAGGAGGCATTGGCTGCCTTCAAGAGTAGTTGATGTCAACTTGGAAAGCAGTGGACATCTCAAATTTTGACCTCCAATTCCTCATTTCTAGCTAATGAGGATCACTTCGTTTCTGATGCTGGTACTTTAGAGGATTCAGTGGTTGGATTCAGGCAAGTCTTATGGGTGCAGTAATACTACTTTAGGTTTCTCATTCCTTTGTGTGCGTTAGTGAACAAATGTCTACCTTGTATTCAAGTATTCATATCCATCTTAATATTTTGCAGAGGAACATTTTTATGGTGTATTAAATGCTGAAGATGCCCTCAGAGGAATTATTTTGTATTATTGTTCAACACTGTATTTACTTTGTATTACAATGAAATATTATGCTATCTGTCAGTAATTGTTGATTTCCCCCCATTTGCTTGTTATTTAATCGATACCAAAAACCACTTAGCATGTT

Ace file of the UniGene sp_v1.1_unigene229 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Magnesium-chelatase subunit chlD, chloroplastic n=2 Tax=Arabidopsis RepID=CHLD_ARATH	-	-	72%
FL-Next	sp=Magnesium-chelatase subunit chlD, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	74%
Blast2go	magnesium-chelatase subunit chloroplast	-	-	88%

Source	GOs	Term	Type	e value	Identity
Blast2go	chloroplast	GO:0009507	Cellular Component	0.0	88%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Magnesium chelatase, ChlI subunit	IPR000523	-	0.0	88%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: sp_plants

Fln subject: Q9SJE1

Fln msg: Separated hits, possible frame ERROR between 1682 and 1703, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 685 - 760

Fln protein: ENEYGRQYFPLAAVVGQDAIKTALLLGAVDNAIGGIAISGKRGTAKTVMARGLHAIIPPIEVVAGSIANSDPLCPEEWEEGLENRLKYNANGTLKTDIIKSPFVQIPLGVTEDRLLGSVDVEESVKSGTTVFQPGLLADAHRGVLYVDEINLLDEGISNLLLNVLTEGVNVVEREGISFRHPCKPLLIATYNPEEGSVREHLLDRIAINLSADVPMEFDDRVSAVDLAIQFQEKSKQVFRSVEEGTDSAKTQIIFAREYLKEVSISRDQIKYLVMEALRGGCQGHRAELYASKVARSLAALEGRDKVGVDDLKKAVELVIIPRSTVNENPPEKQNQPPPPPPPPPQNQDPAEEQNEEDKDEEDDGDNEQQEEEQIPDEFIFDAEGGLVDSKLLFFAQQAQRRKGKAGRAKNVIFSEDRGRYIKPMLPKGPIRRLAIDATLRAAAPYQKLRRQxxxxxxxxQRVFVEKTDMRAKRMARKAGALVIFVVDASGSMALNRMQNAKGAAMQLLSESYTSRDQVSIIPFRGDAADVLLPPSRSIAMARKRLERLPCGGGSPLAHGLSMAVRVGLNAEKSGDVGRVMIVAITDGRANISLKRSTDPDSSATDSPKPSTQELKDEILEIAGKIYKAGMSLLVIDTENKFVSTGFAKEIARVAQGKYYYLPNASDAVISSATKEALAAFKSS*

Protein Length: 686

Fln nts: ATGGGGGGGATTTCATTTACGCTATCCACTGCAACACCCCGCTACCGTGTCCTGTAGAAATAAAATAAAAATATACTCAATTGGAATGGGGACTTCGAAGTGAGTGAGTGGTGCACGGCCATGGCACTCTCCGCCTCGGCCACGGCCTCATATACCAGGGGAAGAAGATTAGCCTTCAAAGAAACTTCATGCCTATCATCATTTAAAGGATCAGGATTTCTTTTTAACAAATGCTGGTTCCCACAGACGCGCTTTCCTCGGCGACTCGGGTTTGCTCGTTGTAATACAAGCAATGGAACAGCTCTTTCTGCCCCCGACTTTGAGAAAACGAGTATGGGCGCCAATATTTTCCTCTCGCCGCAGTTGTGGGACAGGATGCCATCAAGACAGCATTACTACTTGGAGCTGTTGATAATGCCATTGGTGGCATTGCCATTTCAGGGAAAAGAGGGACTGCCAAGACAGTAATGGCACGTGGATTGCATGCTATAATTCCACCTATTGAAGTTGTTGCGGGGTCTATTGCCAACTCTGACCCATTGTGTCCCGAAGAATGGGAAGAAGGACTAGAGAACCGACTAAAGTATAATGCTAATGGTACATTGAAAACAGATATAATAAAATCTCCATTTGTGCAGATACCACTTGGTGTAACAGAGGATAGGCTTTTAGGTTCAGTGGATGTAGAGGAGTCAGTCAAATCTGGAACAACGGTATTCCAGCCAGGTCTTCTTGCTGATGCTCACAGAGGAGTCCTATATGTTGATGAGATAAATCTTCTTGATGAAGGTATAAGCAATTTGCTTCTAAATGTTCTCACAGAAGGAGTTAATGTTGTGGAAAGAGAAGGAATCAGTTTTCGACATCCCTGCAAGCCACTTCTAATTGCTACGTACAATCCCGAGGAAGGTTCTGTACGAGAACATTTACTTGATCGCATTGCAATCAACCTCAGTGCTGATGTTCCCATGGAATTTGATGACCGTGTATCAGCAGTTGATCTTGCAATCCAATTCCAGGAAAAGTCCAAGCAAGTATTTCGGTCAGTTGAAGAAGGAACTGATTCAGCAAAGACACAGATCATTTTTGCAAGGGAATACCTAAAGGAAGTGAGCATTAGTAGGGATCAAATAAAGTATCTTGTTATGGAAGCCTTACGTGGTGGTTGTCAGGGTCACAGAGCTGAACTGTATGCTTCTAAAGTTGCAAGATCATTGGCCGCTCTTGAGGGTCGTGATAAAGTTGGGGTTGATGATCTCAAGAAAGCAGTAGAGCTGGTGATAATACCTCGTTCCACAGTTAATGAAAATCCACCGGAAAAACAGAACCAGCCTCCTCCACCGCCACCACCACCACCACAAAATCAGGATCCTGCAGAAGAACAAAACGAAGAAGATAAAGATGAGGAGGATGATGGTGATAATGAACAGCAAGAAGAAGAGCAAATACCAGATGAGTTTATCTTTGATGCAGAAGGTGGTTTGGTGGACTCAAAGCTGCTCTTCTTTGCACAACAGGCACAGAGGCGAAAGGGCAAAGCTGGTCGTGCCAAAAATGTAATATTTTCCGAGGATAGAGGACGATACATCAAGCCCATGCTTCCAAAGGGACCCATACGGAGGCTAGCCATTGATGCAACCCTAAGAGCAGCAGCACCCTATCAAAAATTGCGAAGACAGAGxxxxxxxxxxxxxxxxxxxxxACAGAGGGTTTTTGTGGAGAAGACTGATATGAGAGCCAAGCGAATGGCCCGCAAAGCGGGAGCTCTGGTGATATTTGTTGTTGATGCCAGTGGAAGTATGGCACTGAATCGCATGCAAAATGCAAAAGGTGCTGCCATGCAACTTCTTTCTGAGAGCTATACAAGCAGAGATCAGGTATCAATTATTCCATTTCGTGGAGATGCAGCAGATGTCCTCCTACCACCATCTCGATCAATTGCAATGGCAAGAAAACGACTAGAGAGGCTACCTTGTGGTGGTGGCTCTCCCTTGGCTCATGGTCTTAGTATGGCTGTTCGAGTAGGATTAAATGCTGAGAAGAGTGGAGATGTTGGACGTGTGATGATAGTGGCTATCACAGATGGAAGGGCTAACATATCATTGAAAAGATCTACTGATCCAGATTCTAGTGCTACCGATTCACCAAAACCTTCCACACAAGAGTTGAAGGACGAGATATTAGAAATAGCAGGAAAGATTTACAAGGCAGGAATGTCCCTTCTTGTGATCGACACAGAGAATAAGTTTGTATCCACAGGGTTTGCAAAGGAGATTGCTAGAGTTGCTCAGGGGAAGTATTACTATTTACCTAATGCTTCCGATGCAGTTATATCCTCTGCAACAAAGGAGGCATTGGCTGCCTTCAAGAGTAG___TTGATGTCAACTTGGAAAGCAGTGGACATCTCAAATTTTGACCTCCAATTCCTCATTTCTAGCTAATGAGGATCACTTCGTTTCTGATGCTGGTACTTTAGAGGATTCAGTGGTTGGATTCAGGCAAGTCTTATGGGTGCAGTAATACTACTTTAGGTTTCTCATTCCTTTGTGTGCGTTAGTGAACAAATGTCTACCTTGTATTCAAGTATTCATATCCATCTTAATATTTTGCAGAGGAACATTTTTATGGTGTATTAAATGCTGAAGATGCCCTCAGAGGAATTATTTTGTATTATTGTTCAACACTGTATTTACTTTGTATTACAATGAAATATTATGCTATCTGTCAGTAATTGTTGATTTCCCCCCATTTGCTTGTTATTTAATCGATACCAAAAACCACTTAGCATGTT

Fln Alignment:

sp_v1.1_unigene229___YGRQYFPLAAVVGQDAIKTALLLGAVDNAIGGIAISGKRGTAKTVMARGLHAIIPPIEVVAGSIANSDPLCPEEWEEGLENRLKYNANGTLKTDIIKSPFVQIPLGVTEDRLLGSVDVEESVKSGTTVFQPGLLADAHRGVLYVDEINLLDEGISNLLLNVLTEGVNVVEREGISFRHPCKPLLIATYNPEEGSVREHLLDRIAINLSADVPMEFDDRVSAVDLAIQFQEKSKQVFRSVEEGTDSAKTQIIFAREYLKEVSISRDQIKYLVMEALRGGCQGHRAELYASKVARSLAALEGRDKVGVDDLKKAVELVIIPRS----TVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQIPDEFIFDAEGGLVDSKLLFFAQQAQRRKGKAGRAKNVIFSEDRGRYIKPMLPKGPIRRLAIDATLRAAAPYQKLRRQRxxxxxxxQRVFVEKTDXXXXXXXXXAGALVIFVVDASGSMALNRMQNAKGAAMQLLSESYTSRDQVSIIPFRGDAADVLLPPSRSIAMARKRLERLPCGGGSPLAHGLSMAVRVGLNAEKSGDVGRVMIVAITDGRANISLKRXXXXXXXXXXXXXXXTQELKDEILEIAGKIYKAGMSLLVIDTENKFVSTGFAKEIARVAQGKYYYLPNASDAVISSATKEALAAFKSS
Q9SJE1_____________YGRQFFPLAAVVGQEGIKTALLLGAVDREIGGIAISGRRGTAKTVMARGLHEILPPIEVVVGSISNADPACPDEWEDDLDERIEYNADNTIKTEIVKSPFIQIPLGVTEDRLIGSVDVEESVKRGTTVFQPGLLAEAHRGVLYVDEINLLDEGISNLLLNVLTDGVNIVEREGISFRHPCKPLLIATYNPEEGAVREHLLDRVAINLSADLPMSFEDRVAAVGIATQFQERCNEVFRMVNEETETAKTQIILAREYLKDVKISREQLKYLVLEAVRGGVQGHRAELYAARVAKCLAAIEGREKVTIDDLRKAVELVILPRSSLDETPPEQQNQPPPPPPPPQNSESGEEENEEEQEEEEEDESNEENENEQQQDQIPEEFIFDAEGGLVDEKLLFFAQQAQKRRGKAGRAKNVIFSEDRGRYIKPMLPKGPVKRLAVDATLRAAAPYQKLRREKxxxxxxxRKVFVEKTDMRAKRMARKAGALVIFVVDASGSMALNRMQNAKGAALKLLAESYTSRDQVSIIPFRGDAAEVLLPPSRSIAMARNRLERLPCGGGSPLAHGLTTAVRVGLNAEKSGDVGRIMIVAITDGRANITLKRSTDPESIAPDAPRPTSKELKDEILEVAGKIYKAGMSLLVIDTENKFVSTGFAKEIARVAQGKYYYLPNASDAVISATTRDALSDLKNS

SNPs (tot: 45)

Position	A %	C %	G %	T %	Probability
411	66		33		0.997452
732	88	11			0.923564
734	11		88		0.923564
759	83	16			0.994512
760		16		83	0.994512
761	16			83	0.994512
901			85	14	0.993533
904	71		28		1.0
906	85		14		0.993533
910		14	85		0.993533
912			14	85	0.993533
913	85	14			0.993533
915	14	85			0.993533
916	14			85	0.993533
918	85		14		0.993533
921	14			85	0.993533
923			14	85	0.993533
924	14		85		0.993533
926			14	85	0.993533
1081	80	20			0.995493
1084		80	20		0.995493
1087		60	40		1.0
1088	40			60	1.0
1091			40	60	1.0
1092	40			60	1.0
1093		40		60	1.0
1094	40	60			1.0
1095			40	60	1.0
1098	40	60			1.0
1099	40			60	1.0
1100			40	60	1.0
1101			40	60	1.0
1106	60		40		1.0
1108	60	40			1.0
1952		75		25	0.996473
2056	66	33			0.997452
2060			66	33	0.997452
2266		14	85		0.993533
2317			14	85	0.993533
2488		66	33		0.997452
2698		50	50		1.0
2707		75	25		0.996473
2708		75	25		0.996473
2709		75		25	0.996473
2712	25			75	0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
1431	1446	GTG GTG GTG GTG GTG G	16

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain