SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene74

Length: 2209 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene74
AAACGAACTGGGTTTGCGAGAGCTGGGGACTGGGCAATCCGGCGATGTCTGTTGGGACGTTAGATAGTGGGAGGCAGACTTTCCATGGCGGCGCTGCTGGGCGAGTGGACTGGGACTGGGACATGAATATTGCTACAAACAACAATCCATTGCTCTTATCTTCTCATCACCGCCATCACCACCACCACCATCATGATCATCATGATTCCGATGAGCACAAGCCTCATCTGATGCCCTCCGCCTCGGCTCCGACTTTCCGGCAATACAATGGTGTAATGGGCCCGTTTTCAGGCCACCAGAACAACGCTCTAGGGCTTCTGAATTCTTCCGGGATTCGCAGTTTCGAGGGATCGAACGATGGAATGAATCCCCCGCCCCCTCCTTCGTTCCATCCGGGGTTGCTGTACTCAGAAATGCCTGGATTCGGTGCCGGGGGCTTTGGCTGCGGTGATTCCAGATCTCAATTGCTGGGCATGATGAGCATGCAGCAACAGGCGGAACATCATCACCATCATCATAAAGAAGGTGGCTTCCACGACATAGTGAAACGGGAGGAGCTTTGCGCTGGAGATTTCCAGGCGAGAATAGGGCTGAATCTCGGACACAGAACTATTTTTCCGCCGAAGATACTGCCGTTAGTAGACTCTACAAGCGCCCGCGTGCGATTCCTCCCGCTTCACAAGTCCCTCGCTGCCAGGCCGAGGGTTGCAAAGCAGACCTAACAAATGCCAAGCACTATCATCGTCGCCATAAAGTCTGCGAATTTCATTCCAAGGCCACCAGCGTTGTCACAGGCGGCGTCCAGCAGCGTTTCTGCCAACAGTGCAGCCGCTTTCATGTTCTTGCAGAATTCGACGAGGTGAAGCGGAGTTGCCGAAAACGCCTGGCGGATCATAACAGGCGGAGGCGCAAACCGCAGACAAACACGATCGTGCCGACTGTCTCCACTGCGGAAGCTGCAACCGCCTTAAAAGTGGAAGACGACACCAACAATGTCTCAGGAAACTGTAACAATACAGCAAGGGGTACTGTACAGTCTTCTGCTTCTGCTTCTGCTCCTGCAGCTCCAGTTCTGAGAACAGAAGCACAAGCTTTGCTGAATGCAATGTCTTTACCTTTAATACTTCAGAACACCAAGGGATCACAGATGAGCAACAATAATACTAATACTACTAATGCTACTACTAATAATCCTATGTTGCAGCAAGCCAATTCTCAGAAGGTTACTGTAGCGAACCAGCAATACGTGCAGCAAAGCGGAGGAGGAGGACACAAGGGGCCGAATTTATCTTTATCGTCGTCATCTGTGGGAATGAGTTTGTGCAATCAGAGGCAAACAACGGCACAAGATTACAGCGGGCTCGGAACTTGCGCTGCCTTGGCTGAGAACAACTTCAGAATACAGCGGATCGAACCATAGACCTCCTCCGGCCATGGACAATATCCTTCATCCTATTATATCTCATGAGAAGTCAAATGTTGAAGCTTTTTCGGCCGCGTCGGTTCAGAACTTTCTGCCTTTAACAGGCTCTATGAGTTCCAGAGAACTCGGTAGTTCGCAATGGATGGTTGGGAGTAGTGATAGCGCGAGTAGCCTGGAAGCTGGCCAGCAGATGTTCAGCTTGCTGGAACCAGCTGAACATCATAATAGAACCGAGGAACAATCGCATACGGCTCGGCCAGTGGAATATTTGTCGCATCGTCATCAAGGGGGTCTGAATTCCAAAGAACCTGTTGATGGAAATTCCGGAGCAAAATTCTCTGAAATGCAAAATTCGCTGAGACCGTTCGGCCAGTCTATTTATGACGGAAGTATGTGACTCGAACAACAGACGTCAGATCTCTAATATTGATTGGATTAACTGATGAACCGGTGGAGTTCTTGGGACTAGCTTAGCCCTAAAAGCATGCCGCGAGCTTCAGGAACTACTGCTGTTCTTGTTTAATTCGATTCAGGCCATGAGAAGGCTGCATTACTTAATAGGGTTCGGGAGCGTGGCCATGATCATTATTAGGGCAATTCCTCTTTTACTTAACTCTACATTACATTTCAGCATTTTCTGTGGAGTGAAATCTTGTAACAGCAGTCCGATCCTCGACAATGAAGCTCCAGGTCTTTTCCAGGTCTTTTCGTTCTTGTCCATGAATAAGCGAACACGACTTTTCCTCATGAATAATATGTTCTTCATTAATTTGTTCCTAGCTTATC

Ace file of the UniGene sp_v1.1_unigene74 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Predicted protein n=1 Tax=Physcomitrella patens subsp. patens RepID=A9TKM8_PHYPA	-	-	61%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	61%
Blast2go	protein	-	-	72%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NSA8

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is longer than subject: 300 - 198

Fln protein: RRWLPRHSETGGALRWRFPGENRAESRTQNYFSAEDTAVSRLYKRPRAIPPASQVPRCQAEGCKADLTNAKHYHRRHKVCEFHSKATSVVTGGVQQRFCQQCSRFHVLAEFDEVKRSCRKRLADHNRRRRKPQTNTIVPTVSTAEAATALKVEDDTNNVSGNCNNTARGTVQSSASASAPAAPVLRTEAQALLNAMSLPLILQNTKGSQMSNNNTNTTNATTNNPMLQQANSQKVTVANQQYVQQSGGGGHKGPNLSLSSSSVGMSLCNQRQTTAQDYSGLGTCAALAENNFRIQRIEP*

Protein Length: 301

Fln nts: AAACGAACTGGGTTTGCGAGAGCTGGGGACTGGGCAATCCGGCGATGTCTGTTGGGACGTTAGATAGTGGGAGGCAGACTTTCCATGGCGGCGCTGCTGGGCGAGTGGACTGGGACTGGGACATGAATATTGCTACAAACAACAATCCATTGCTCTTATCTTCTCATCACCGCCATCACCACCACCACCATCATGATCATCATGATTCCGATGAGCACAAGCCTCATCTGATGCCCTCCGCCTCGGCTCCGACTTTCCGGCAATACAATGGTGTAATGGGCCCGTTTTCAGGCCACCAGAACAACGCTCTAGGGCTTCTGAATTCTTCCGGGATTCGCAGTTTCGAGGGATCGAACGATGGAATGAATCCCCCGCCCCCTCCTTCGTTCCATCCGGGGTTGCTGTACTCAGAAATGCCTGGATTCGGTGCCGGGGGCTTTGGCTGCGGTGATTCCAGATCTCAATTGCTGGGCATGATGAGCATGCAGCAACAGGCGGAACATCATCACCATCATCATAAAGAAGGTGGCTTCCACGACATAGTGAAACGGGAGGAGCTTTGCGCTGGAGATTTCCAGGCGAGAATAGGGCTGAATCTCGGACACAGAACTATTTTTCCGCCGAAGATACTGCCGTTAGTAGACTCTACAAGCGCCCGCGTGCGATTCCTCCCGCTTCACAAGTCCCTCGCTGCCAGGCCGAGGGTTGCAAAGCAGACCTAACAAATGCCAAGCACTATCATCGTCGCCATAAAGTCTGCGAATTTCATTCCAAGGCCACCAGCGTTGTCACAGGCGGCGTCCAGCAGCGTTTCTGCCAACAGTGCAGCCGCTTTCATGTTCTTGCAGAATTCGACGAGGTGAAGCGGAGTTGCCGAAAACGCCTGGCGGATCATAACAGGCGGAGGCGCAAACCGCAGACAAACACGATCGTGCCGACTGTCTCCACTGCGGAAGCTGCAACCGCCTTAAAAGTGGAAGACGACACCAACAATGTCTCAGGAAACTGTAACAATACAGCAAGGGGTACTGTACAGTCTTCTGCTTCTGCTTCTGCTCCTGCAGCTCCAGTTCTGAGAACAGAAGCACAAGCTTTGCTGAATGCAATGTCTTTACCTTTAATACTTCAGAACACCAAGGGATCACAGATGAGCAACAATAATACTAATACTACTAATGCTACTACTAATAATCCTATGTTGCAGCAAGCCAATTCTCAGAAGGTTACTGTAGCGAACCAGCAATACGTGCAGCAAAGCGGAGGAGGAGGACACAAGGGGCCGAATTTATCTTTATCGTCGTCATCTGTGGGAATGAGTTTGTGCAATCAGAGGCAAACAACGGCACAAGATTACAGCGGGCTCGGAACTTGCGCTGCCTTGGCTGAGAACAACTTCAGAATACAGCGGATCGAACCATAG___ACCTCCTCCGGCCATGGACAATATCCTTCATCCTATTATATCTCATGAGAAGTCAAATGTTGAAGCTTTTTCGGCCGCGTCGGTTCAGAACTTTCTGCCTTTAACAGGCTCTATGAGTTCCAGAGAACTCGGTAGTTCGCAATGGATGGTTGGGAGTAGTGATAGCGCGAGTAGCCTGGAAGCTGGCCAGCAGATGTTCAGCTTGCTGGAACCAGCTGAACATCATAATAGAACCGAGGAACAATCGCATACGGCTCGGCCAGTGGAATATTTGTCGCATCGTCATCAAGGGGGTCTGAATTCCAAAGAACCTGTTGATGGAAATTCCGGAGCAAAATTCTCTGAAATGCAAAATTCGCTGAGACCGTTCGGCCAGTCTATTTATGACGGAAGTATGTGACTCGAACAACAGACGTCAGATCTCTAATATTGATTGGATTAACTGATGAACCGGTGGAGTTCTTGGGACTAGCTTAGCCCTAAAAGCATGCCGCGAGCTTCAGGAACTACTGCTGTTCTTGTTTAATTCGATTCAGGCCATGAGAAGGCTGCATTACTTAATAGGGTTCGGGAGCGTGGCCATGATCATTATTAGGGCAATTCCTCTTTTACTTAACTCTACATTACATTTCAGCATTTTCTGTGGAGTGAAATCTTGTAACAGCAGTCCGATCCTCGACAATGAAGCTCCAGGTCTTTTCCAGGTCTTTTCGTTCTTGTCCATGAATAAGCGAACACGACTTTTCCTCATGAATAATATGTTCTTCATTAATTTGTTCCTAGCTTATC

Fln Alignment:

sp_v1.1_unigene74___KRPRAIP-----PASQVPRCQAEGCKADLTNAKHYHRRHKVCEFHSKATSVVTGGVQQRFCQQCSRFHVLAEFDEVKRSCRKRLADHNRRRRKPQTNTIV
A9NSA8____________KKPKAASSSSSSPRAQPPACQVEKCAADLADAKEYYRRHRVCEQHSKARVVLVLGLQQRFCQQCSRFHVLEEFDEAKRSCRRRLAGHNERRRKTPSDSSV

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
678	40		60		1.0
709		10	90		0.90298
710	90		10		0.90298
711		90		10	0.90298
748	85		14		0.993533
752	85	14			0.993533
753		14		85	0.993533
1190		80	20		0.995493
1192	20			80	0.995493
1193	20			80	0.995493
1969	14		85		0.993533
1996			14	85	0.993533

SSRs (tot: 3)

Start position	End position	Sequence	Length
940	951	TCC TCC TCC TCC	12
1153	1172	AGCAGA AGCAGA AGCAGA AG	20
2020	2033	TGG TGG TGG TGG TG	14

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain