SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene20

Length: 2390 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene20
CGTCGTGACCTTGGCTGTCACTGTAACTCCACAGTTTGTATAATAGACACCCCTCGGCGGAGAGGCCAGCAACAATAAATTTCTCTCTTTTCTTTCCTATTTCGATCGAAATTCTTCCTGTGAAGCCTCGTTTCCATGGCTTCTCTGATAGGAATAGTACCGTTGCTGCTCCTTCTGACGCTAATTCCTGCGACCGAGAGCTTCAATGATACAGAGGCATTACTCCAATTCAGAGCCCTAACGGATTCCCACGGCATTCTTGCCAAAAACTGGAGCTCGGCCGATGCCTGCACTGGGAAATGGCAGGGCATAACCTGCAATAAAGGCAGAGTCTTCTTCATTTCGCTTTCGGGCCATGAGTTACGAGGCCCTATCGATCCTCTTTCTGCGCTCGACCAGCTCCGGGTTCTTGATCTTCAGGGAAATCGGCTCAACGGCAGCTTGGATCCATTGTCAAATTGCTACAACCTCAAATTGCTCTACTTGGCTTCCAATGACTTCTCGGGTCTGATTCCTGCTGATCTTGCTCGGCTCAGGCGTCTGGTGAGGCTCGACGCTTCCGATAACAATCTCCGCGGATCGATTCCGCCGGTGATACGGAACCTTACGCGGCTGGTAACTCTGCGCCTGCAGAACAACATTCTGTCAGGGCCGTTGCCGGATCTCAGCTTCTTGCGGGACTTAAATGTTTCCAATAATGAACTTTCTGGCCAAGTACCCCGCCAGCTTCTGGAGAAGTTCCCGGCTTCGGCCTTCGCAGGCAATGCTGGGCTCTGCGGACGGAGTCCTTTTCCGGCCTGTACCATTCCGCCACCGGTGGCTGTAGTGGTGCCGTCGAATCCGAGCTCCGACCCGGGATCGAGTACTCCCGTGGACACGAGATCCAAAACTAGGGCTCTCAGCACGGGGGCCGTCATTGCCATTGTGGTCGGTGATGCTGCCGTTCTGATTTTGATAACGCTCATTTTTCTGGTGTATTACTGGAAGAAATACGTCAGGGAGGCCGAGGTTGCTGGAAAAACGGCCTCCGAGAAGATGGATTTCTCCGGTGGCGCGCAGACGCCAGAGATGGAGAGGAGTAAACTTGTGTTCTTTGATAGGAAGAAGCAGTTTGAGTTGGAGGATCTGCTGAGGGCGTCTGCCGAGATGTTGGGGAAGGGGAGCTTTGGGACTGCTTATAAAGCCGTTCTTGATGATGGCTGTGTTGTTGCTGTCAAGAGATTAAAGGACGTTAATCCTTCTGGGAAGAAGGAATTCGATCAGCACATGGAGTTGATTGGGAAATTGAGGCATCCCCACATAGTTCGTCTCAGAGCTTATTATTATGCAAAAGAGGAGAAGCTTCTGGTTTATGATTATCTTCCCAATGGGAGCTTGTATTCTCTTCTACACGGGAACAGAGGTCCAGGAAGAACACCATTGGACTGGACTACAAGAATCAGTCTAGTCTTAGGAGCTGCTCGAGGTCTTGCTTACATCCATGAAGAATATGGGTCTTTGAAGATTCCACATGGGAATATCAAATCCTCCAATATTCTCTTGGACAAGAATGGTGTTGCATGCATTTCTGATTTTGGGTTGGCCCTGCTCCTGAACCCCTCGCATATTCATGCTTCATCTAGATATGTAGGGTACAGAGCACCTGAGCAGGCAGAAACCAAAAAATTGTCCCAAAAGGCCGATGTGTACAGCTTTGGTGTGTTACTCCTTGAGGTCTTGACTGGAAAAGCTCCTATGCACTATCATTCGCAAGAAGAAGGTGTTGATCTTCCAAAATGGGTTCAGTCCATTGTCCGGGAAGAATGGACTGCTGAAGTGTTCGACCTTGAATTGATGAGGTACAAGAACATTGAGGAGGAGATGGTTTCGATGTTGCAGATTGCTTTGAGTTGTGTTTCACAGTCACCCGAACAACGTCCTAAGATGGGTGAAGTTGTTAGGTTGATTGAGGACATCAGAGTTGAGCAATCTCCTTTGGGTGAATGGGATGAGTCTTACTCTGTTTCTCCTTCTGCCTCTGAAGATGTTGTAACAAGCCACTGAAACGGATGGTTTAGCTAGATTTGGAGTGTGTCATGTTATGTTTGTTTCCCATTGTCTTGTTAGGTATCAAGAGCTATTGATAGATTTAATGTATTATATCTTGGCTTGGGTAGAGTCTGTACGTGATTGCTCGAAAGATAATTCTATGTATTGGTTATGTAGTTCCACAGCATACATTCTTTATGGTTTAAGTAATTTCAGCATTGCGGTTTATTAATGGAGTGGTGGTTCGTTGGATAATTTTTCTGCCAAGAGTTGCTCGGCATTTTTTGCTATATTGTCATAAATTACTGGTTCTAATTACTAGCTCAAGATGTATTTTGGAAGTGCAATCAATTAATGGCAAT

Ace file of the UniGene sp_v1.1_unigene20 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Protein kinase APK1A, chloroplast precursor, putative (EC:2.7.11.30)	-	-	57%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	64%
Blast2go	protein kinase chloroplast	-	-	68%

Source	GOs	Term	Type	e value	Identity
Blast2go	protein binding	GO:0005515	Molecular Function	0.0	68%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	68%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LN40

Fln msg: your sequence is longer than subject: 636 - 340

Fln protein: MASLIGIVPLLLLLTLIPATESFNDTEALLQFRALTDSHGILAKNWSSADACTGKWQGITCNKGRVFFISLSGHELRGPIDPLSALDQLRVLDLQGNRLNGSLDPLSNCYNLKLLYLASNDFSGLIPADLARLRRLVRLDASDNNLRGSIPPVIRNLTRLVTLRLQNNILSGPLPDLSFLRDLNVSNNELSGQVPRQLLEKFPASAFAGNAGLCGRSPFPACTIPPPVAVVVPSNPSSDPGSSTPVDTRSKTRALSTGAVIAIVVGDAAVLILITLIFLVYYWKKYVREAEVAGKTASEKMDFSGGAQTPEMERSKLVFFDRKKQFELEDLLRASAEMLGKGSFGTAYKAVLDDGCVVAVKRLKDVNPSGKKEFDQHMELIGKLRHPHIVRLRAYYYAKEEKLLVYDYLPNGSLYSLLHGNRGPGRTPLDWTTRISLVLGAARGLAYIHEEYGSLKIPHGNIKSSNILLDKNGVACISDFGLALLLNPSHIHASSRYVGYRAPEQAETKKLSQKADVYSFGVLLLEVLTGKAPMHYHSQEEGVDLPKWVQSIVREEWTAEVFDLELMRYKNIEEEMVSMLQIALSCVSQSPEQRPKMGEVVRLIEDIRVEQSPLGEWDESYSVSPSASEDVVTSH*

Protein Length: 637

Fln nts: CGTCGTGACCTTGGCTGTCACTGTAACTCCACAGTTTGTATAATAGACACCCCTCGGCGGAGAGGCCAGCAACAATAAATTTCTCTCTTTTCTTTCCTATTTCGATCGAAATTCTTCCTGTGAAGCCTCGTTTCC_-_ATGGCTTCTCTGATAGGAATAGTACCGTTGCTGCTCCTTCTGACGCTAATTCCTGCGACCGAGAGCTTCAATGATACAGAGGCATTACTCCAATTCAGAGCCCTAACGGATTCCCACGGCATTCTTGCCAAAAACTGGAGCTCGGCCGATGCCTGCACTGGGAAATGGCAGGGCATAACCTGCAATAAAGGCAGAGTCTTCTTCATTTCGCTTTCGGGCCATGAGTTACGAGGCCCTATCGATCCTCTTTCTGCGCTCGACCAGCTCCGGGTTCTTGATCTTCAGGGAAATCGGCTCAACGGCAGCTTGGATCCATTGTCAAATTGCTACAACCTCAAATTGCTCTACTTGGCTTCCAATGACTTCTCGGGTCTGATTCCTGCTGATCTTGCTCGGCTCAGGCGTCTGGTGAGGCTCGACGCTTCCGATAACAATCTCCGCGGATCGATTCCGCCGGTGATACGGAACCTTACGCGGCTGGTAACTCTGCGCCTGCAGAACAACATTCTGTCAGGGCCGTTGCCGGATCTCAGCTTCTTGCGGGACTTAAATGTTTCCAATAATGAACTTTCTGGCCAAGTACCCCGCCAGCTTCTGGAGAAGTTCCCGGCTTCGGCCTTCGCAGGCAATGCTGGGCTCTGCGGACGGAGTCCTTTTCCGGCCTGTACCATTCCGCCACCGGTGGCTGTAGTGGTGCCGTCGAATCCGAGCTCCGACCCGGGATCGAGTACTCCCGTGGACACGAGATCCAAAACTAGGGCTCTCAGCACGGGGGCCGTCATTGCCATTGTGGTCGGTGATGCTGCCGTTCTGATTTTGATAACGCTCATTTTTCTGGTGTATTACTGGAAGAAATACGTCAGGGAGGCCGAGGTTGCTGGAAAAACGGCCTCCGAGAAGATGGATTTCTCCGGTGGCGCGCAGACGCCAGAGATGGAGAGGAGTAAACTTGTGTTCTTTGATAGGAAGAAGCAGTTTGAGTTGGAGGATCTGCTGAGGGCGTCTGCCGAGATGTTGGGGAAGGGGAGCTTTGGGACTGCTTATAAAGCCGTTCTTGATGATGGCTGTGTTGTTGCTGTCAAGAGATTAAAGGACGTTAATCCTTCTGGGAAGAAGGAATTCGATCAGCACATGGAGTTGATTGGGAAATTGAGGCATCCCCACATAGTTCGTCTCAGAGCTTATTATTATGCAAAAGAGGAGAAGCTTCTGGTTTATGATTATCTTCCCAATGGGAGCTTGTATTCTCTTCTACACGGGAACAGAGGTCCAGGAAGAACACCATTGGACTGGACTACAAGAATCAGTCTAGTCTTAGGAGCTGCTCGAGGTCTTGCTTACATCCATGAAGAATATGGGTCTTTGAAGATTCCACATGGGAATATCAAATCCTCCAATATTCTCTTGGACAAGAATGGTGTTGCATGCATTTCTGATTTTGGGTTGGCCCTGCTCCTGAACCCCTCGCATATTCATGCTTCATCTAGATATGTAGGGTACAGAGCACCTGAGCAGGCAGAAACCAAAAAATTGTCCCAAAAGGCCGATGTGTACAGCTTTGGTGTGTTACTCCTTGAGGTCTTGACTGGAAAAGCTCCTATGCACTATCATTCGCAAGAAGAAGGTGTTGATCTTCCAAAATGGGTTCAGTCCATTGTCCGGGAAGAATGGACTGCTGAAGTGTTCGACCTTGAATTGATGAGGTACAAGAACATTGAGGAGGAGATGGTTTCGATGTTGCAGATTGCTTTGAGTTGTGTTTCACAGTCACCCGAACAACGTCCTAAGATGGGTGAAGTTGTTAGGTTGATTGAGGACATCAGAGTTGAGCAATCTCCTTTGGGTGAATGGGATGAGTCTTACTCTGTTTCTCCTTCTGCCTCTGAAGATGTTGTAACAAGCCACTGA___AACGGATGGTTTAGCTAGATTTGGAGTGTGTCATGTTATGTTTGTTTCCCATTGTCTTGTTAGGTATCAAGAGCTATTGATAGATTTAATGTATTATATCTTGGCTTGGGTAGAGTCTGTACGTGATTGCTCGAAAGATAATTCTATGTATTGGTTATGTAGTTCCACAGCATACATTCTTTATGGTTTAAGTAATTTCAGCATTGCGGTTTATTAATGGAGTGGTGGTTCGTTGGATAATTTTTCTGCCAAGAGTTGCTCGGCATTTTTTGCTATATTGTCATAAATTACTGGTTCTAATTACTAGCTCAAGATGTATTTTGGAAGTGCAATCAATTAATGGCAAT

Fln Alignment:

sp_v1.1_unigene20___GGAQTPEMERSKLVFFDRKKQ-FELEDLLRASAEMLGKGSFGTAYKAVLDDGCVVAVKRLKDVNPSGKKEFDQHMELIGKLRHPHIVRLRAYYYAKEEKLLVYDYLPNGSLYSLLHGNRGPGRTPLDWTTRISLVLGAARGLAYIHEEYGSLKIPHGNIKSSNILLDKNGVACISDFGLALLLNPSHIHASSRYVGYRAPEQAETKKLSQKADVYSFGVLLLEVLTGKAPMHYHSQEEGVDLPKWVQSIVREEWTAEVFDLELMRYKNIEEEMVSMLQIALSCVSQSPEQRPKMGEVVRLIEDIR
B8LN40____________GSAQ--EAERNKLVFFEGSQYTFDLEDLLRASAEVLGKGSVGTAYKAVLEDGTTVVVKRLKDV-AANRKDFEQQMELVGRIRHRNLVPLRAFYYSKDEKLLVYDYMPTGSLSALLHGSRGSGRTPLDWDTRMRIALGAARGISHIHEEGGG-KFTHGNIKSSNVLLTTDLDGCVSDFGLVPLFSAAA--AANRIAGYRAPEVIETRKVTQKSDVYSFGVLLLELLTGKAPNQASLNDEGIDLPRWVQSVVREEWTAEVFDVELMRYQNIEEEMVQLLQIAMACVAAVPDQRPRMQDVVKMIEDMR

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
1646	16	83			0.999991
1851	80		20		0.995493
1878	80		20		0.995493
1900	20		80		0.995493
2070	66			33	1.0
2235		16		83	0.999991
2257			54	45	1.0
2289	22		77		0.999999
2325		20		80	0.995493
2326		20	80		0.995493
2327		20	80		0.995493
2329	20	80			0.995493
2340			75	25	0.996473
2354	66			33	0.997452
2355		66	33		0.997452
2357	66		33		0.997452
2362	33			66	0.997452
2364		33		66	0.997452
2365		33	66		0.997452
2366	33		66		0.997452
2369			33	66	0.997452
2371	66	33			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain