SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene160405

Length: 447 nt

UniGene Fasta

>sp_v3.0_unigene160405
AGGAGGAGGATGGTATATATCTGGGTGAGGTCTGCCACAAGAAGAATTATCATCTTCTTGTGAGTAGTATAATCGTAATTATTACCATCTTCAGCAGCAATGTCTATGACAAGCATCCCCGAAGTGGAAGTTGAAATGGGCTCGGCATCGCCTAACGCCAGGACTGATGCCAGGACTGTTCGAGCCAGCGTTGTTCAGGCCTCCACTGTCTTCTACGACACTCCTGCTACTCTCGATAAAGCTGAAAGATTGATAGCAGAAGGTGCTGCTTGTGGGTCACAGTTGTTTGTGTTTCCCGAAGCTTTTATCGGTGGCTATCCTCGAGGTTCTAGTTTGGTGTTGTAATGGATATTATATCTTTGAAGGGTCGAGAGGAGTTTCGTAAATACCATGCTTCTGCTATTGATGTGCCAGGTCCAGAAGTGGAGAGGATAGCAGCTGTAGCTG

Ace file of the UniGene sp_v3.0_unigene160405

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	PREDICTED: similar to Bifunctional nitrilase/nitrile hydratase NIT4B n=1 Tax=Vitis vinifera RepID=UPI0001985AF6	-	-	4.0e-30	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	82%
Sma3	Nitrilase, putative	-	-	3.204e-20	-

Source	ECs	Term	Type	e value	Identity
Sma3	Nitrilase.	EC:3.5.5.1	-	2.794e-36	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Tryptophan metabolism	00380		2.794e-36	%
Sma3	Cyanoamino acid metabolism	00460		2.794e-36	%
Sma3	Aminobenzoate degradation	00627		2.794e-36	%
Sma3	Styrene degradation	00643		2.794e-36	%
Sma3	Nitrogen metabolism	00910		2.794e-36	%
Sma3	Metabolic pathways	01100		2.794e-36	%
Sma3	Cyanoalanine nitrilase.	EC:3.5.5.4	-	8.136e-12	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Cyanoamino acid metabolism	00460		8.136e-12	%

Source	Gene names
Sma3	At3g44300; At3g44320; At5g22300; BrNIT1; BrNIT2; GSVIVT00003612001; GSVIVT00003674001; GSVIVT00003679001; GSVIVT00003683001; GSVIVT00003685001; GSVIVT00003689001; GSVIVT00003691001; GSVIVT00003693001; GSVIVT00003699001; GSVIVT00003701001; GSVIVT0003730000

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	nitrilase activity	GO:0000257	Molecular Function	-
Sma3	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	GO:0016810	Molecular Function	-
Sma3	nitrile hydratase activity	GO:0018822	Molecular Function	-
Sma3	cyanoalanine nitrilase activity	GO:0047427	Molecular Function	-
Sma3	3-cyanoalanine hydratase activity	GO:0047558	Molecular Function	-
Sma3	nitrogen compound metabolic process	GO:0006807	Biological Process	-
Sma3	indoleacetic acid biosynthetic process	GO:0009684	Biological Process	-
Sma3	cellular response to sulfate starvation	GO:0009970	Biological Process	-
Sma3	cyanide metabolic process	GO:0019499	Biological Process	-
Sma3	glucosinolate catabolic process	GO:0019762	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	detoxification of nitrogen compound	GO:0051410	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Nitrilase/cyanide hydratase, conserved site	IPR000132	-	0.0	-
Sma3	Nitrilase/cyanide hydratase	IPR003010	-	0.0	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT5G22300.1	NIT4, AtNIT4 nitrilase 4 chr5:7379401-7381764 FORWARD LENGTH=355	8.0e-30	65%
RefSeq	Arabidopsis thaliana	NP_197622.1	bifunctional nitrilase/nitrile hydratase NIT4 [Arabidopsis thaliana]	1.0e-29	65%
RefSeq	Populus trichocarpa	XP_002328237.1	nitrilase 1 [Populus trichocarpa]	1.0e-31	73%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LLF1

Fln msg: ERROR#3, very serious frame error, Overlapping hits, possible frame ERROR between 333 and 333, Distance to subject end: 79 aas, your sequence is shorter than subject: 116 - 191

Fln protein: MSMTSIPEVEVEMGSASPNARTDARTVRASVVQASTVFYDTPATLDKAERLIAEGAACGSQLFVFPEAFIGGYPRGSxxGVVMDIISLKGREEFRKYHASAIDVPGPEVERIAAVA

Protein Length: 117

Fln nts: AGGAGGAGGATGGTATATATCTGGGTGAGGTCTGCCACAAGAAGAATTATCATCTTCTTGTGAGTAGTATAATCGTAATTATTACCATCTTCAGCAGCA_-_ATGTCTATGACAAGCATCCCCGAAGTGGAAGTTGAAATGGGCTCGGCATCGCCTAACGCCAGGACTGATGCCAGGACTGTTCGAGCCAGCGTTGTTCAGGCCTCCACTGTCTTCTACGACACTCCTGCTACTCTCGATAAAGCTGAAAGATTGATAGCAGAAGGTGCTGCTTGTGGGTCACAGTTGTTTGTGTTTCCCGAAGCTTTTATCGGTGGCTATCCTCGAGGTTCTxxxxxxGGTGTTGTAATGGATATTATATCTTTGAAGGGTCGAGAGGAGTTTCGTAAATACCATGCTTCTGCTATTGATGTGCCAGGTCCAGAAGTGGAGAGGATAGCAGCTGTAGCTG

Fln Alignment:

HA8LWWM01EX49J___IPEVEVEMGSASPNARTDARTVRASVVQASTVFYDTPATLDKAERLIAEGAACGSQLFVFPEAFIGGYPRGSSFGVVMDIISLKGREEFRKYHASAIDVPGPEVERIAAVA
B8LLF1________________IPEVEVEMRPASPNART----LRATVVQASTVFYDTPATVEKAERLIAEGAAYGSQLLVFPEAFIGGYPRGSNFGVVMGNRSFKGREEFRKYYASAIDVPGPEVERIAAAA

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain