SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene144809

Length: 816 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene144809 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Flavonoid-3'-hydroxylase n=12 Tax=Vitis RepID=Q2UYU6_VITVI	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	70%
Sma3	Flavonoid 3'-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:1.14.-.-	-	7.732e-06	-
Sma3	Flavonoid 3'-monooxygenase.	EC:1.14.13.21	-	3.277e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Flavonoid biosynthesis	00941		3.277e-07	%
Sma3	Flavone and flavonol biosynthesis	00944		3.277e-07	%
Sma3	Biosynthesis of phenylpropanoids	01061		3.277e-07	%
Sma3	Metabolic pathways	01100		3.277e-07	%
Sma3	Biosynthesis of secondary metabolites	01110		3.277e-07	%
Sma3	N-methylcoclaurine 3'-monooxygenase.	EC:1.14.13.71	-	9.686e-08	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Isoquinoline alkaloid biosynthesis	00950		9.686e-08	%
Sma3	Metabolic pathways	01100		9.686e-08	%
Sma3	Biosynthesis of secondary metabolites	01110		9.686e-08	%
Sma3	Flavonoid 3',5'-hydroxylase.	EC:1.14.13.88	-	8.497e-10	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	8.497e-10	%
Sma3	Flavone and flavonol biosynthesis	00944	8.497e-10	%
Sma3	Biosynthesis of secondary metabolites	01110	8.497e-10	%

Source	Gene names
Sma3	5'H; 5'h; Asp-3; At2g45550; At2g45560; At2g45570; At3g61040; At5g44620; CYP706C5; CYP71A9; CYP71AN1; CYP71AN2v1; CYP71AN3; CYP75; CYP75A1; CYP75A12\|F3'5'H1; CYP75A13; CYP75A2; CYP75A3; CYP75A4; CYP75A5; CYP75A6; CYP75A7; CYP75B2; CYP76C1; CYP76C2; CYP76C4

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	flavonoid 3'-monooxygenase activity	GO:0016711	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	flavonoid 3',5'-hydroxylase activity	GO:0033772	Molecular Function	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G07990.1	TT7, CYP75B1, D501 Cytochrome P450 superfamily protein chr5:2560437-2562859 FORWARD LENGTH=513	56%
RefSeq	Arabidopsis thaliana	NP_196416.1	Flavonoid 3'-monooxygenase [Arabidopsis thaliana]	56%
RefSeq	Populus trichocarpa	XP_002319761.1	flavonoid 3'-hydroxylase [Populus trichocarpa]	60%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LME1

Fln msg: Overlapping hits, possible frame ERROR between 571 and 552, STOP codon was not found. Distance to subject end: 5 aas, your sequence is shorter than subject: 272 - 516

Fln protein: GSQGVRHKMKRLHKRMDNLLSQMIKEHQTASRSGGRADFLSVLLALRNDADGEGGKLTDIDIKALLLTLFAAGTDTSSSTVEWAMAELIRHPEMMRRCQEELDSAVNGERRMLKELDLQKLPYLQAVIKETLRLHPSVPLLVPRMSAEACEVEGYYIPKNCRLLVNAWAMQRDPAVWERPLEFxxxxxxxxSTVDVRGNDFEVIPFGAGRRICAGMNMGMRMMQLILASLLRSFTLSLPDGQLPEKLDMAEACSLTLHKAVPLLVVPAARLP

Protein Length: 273

Fln nts: TGGATCACAGGGCGTGCGGCACAAGATGAAAAGACTGCACAAGAGGATGGATAACTTGTTAAGTCAGATGATAAAGGAGCACCAGACGGCGTCACGTAGTGGAGGGAGGGCGGATTTTCTGAGTGTGCTTCTGGCTCTCAGAAATGATGCGGACGGTGAAGGAGGGAAGCTTACGGATATCGATATAAAGGCCCTTTTGCTTACCCTGTTCGCAGCCGGAACGGACACATCCTCGAGCACAGTGGAATGGGCAATGGCAGAGCTGATACGCCACCCGGAGATGATGAGGAGATGCCAGGAGGAACTGGACTCCGCGGTGAATGGGGAGCGGCGAATGCTGAAGGAGTTGGACCTCCAAAAACTCCCTTACTTGCAGGCGGTGATTAAGGAGACATTAAGGCTGCACCCCTCCGTTCCCCTGCTTGTGCCTAGAATGTCAGCGGAGGCGTGCGAGGTAGAGGGGTATTACATTCCCAAGAACTGTCGTCTCCTCGTGAACGCCTGGGCCATGCAGCGTGACCCGGCGGTGTGGGAGAGACCGCTGGAGTTCxxxxxxxxxxxxxxxxxxxxxxxxAGCACTGTCGACGTTAGGGGCAATGATTTCGAGGTGATCCCATTTGGCGCAGGCAGACGAATCTGTGCGGGGATGAACATGGGGATGCGCATGATGCAGCTGATACTTGCCTCCTTGCTTCGTTCTTTCACTTTGTCTCTCCCCGACGGCCAGTTGCCCGAGAAGCTTGACATGGCGGAAGCCTGCAGTCTCACACTGCATAAGGCAGTGCCGCTGCTTGTCGTTCCCGCCGCTCGTCTTCCCC

Fln Alignment:

AllPine_a_rep_c115665___QGVRHKMKRLHKRMDNLLSQMIKEHQTASRSGG-RADFLSVLLALRNDADGEGGKLTDIDIKALLLTLFAAGTDTSSSTVEWAMAELIRHPEMMRRCQEELDSAVNGERRMLKELDLQKLPYLQAVIKETLRLHPSVPLLVPRMSAEACEVEGYYIPKNCRLLVNAWAMQRDPAVWERPLEFxxxxxxxSTVDVRGNDFEVIPFGAGRRICAGMNMGMRMMQLILASLLRSFTLSLPDGQLPEKLDMAEACSLTLHKAVPLLVVPAARLP
B8LME1________________QGVQKKMKKLHSRFDDFFGRILEEHKVAARNGGGKKDFLSVLLALRNDADGEGGKLTDTDMKALLLDLFTAGTDTSASTVEWAMSELIRHPKMMKKCQQEVESAIGQDRRRLKESDIQKLPYLQAVVKETFRLHPSTPLLLPRLAGEACEVEGYYIPKNARLLVNTWGIQRDPDVWERPLEFxxxxxxxSTVDVRGTDFQVIPFGAGRRICAGVSMGIRMVQLMLASLLHSFDWSLPEGQQPENLDMAEAYGLTLQKAVPLLAVPAARLP

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
428		12		87	0.992554
431	12			87	0.992554
444	33		66		1.0
476		66		33	1.0
544		20	80		0.995493
545		20	80		0.995493
546	20		80		0.995497
788		66		33	0.997456

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain