SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene136308

Length: 290 nt

UniGene Fasta

>sp_v3.0_unigene136308
AGTTTTGTTTAACGTTTATTTTGTATACCACAGGATGGATCTGCATCTCAATTTTGGGATTTCTGCAAGGAGCAAGGTCTTAGGAAACCCTGAAGACCTTGGCCAAAACCAACTCTCATAGCATGGAAACTAATTGCATCATATGCAGCTTTTGAAGCAGCACTCCAACTACTTTTACCTGGTGAAAGAGTAACTGGACCTGTTTCTCCTGCAGGAAACATTCCAGTCTATAAGGCAAACGGTGTGCTGGCTTACTTTGTCACATTGACAACTTATATTGCTATCTGGTG

Ace file of the UniGene sp_v3.0_unigene136308

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	7-dehydrocholesterol reductase, putative n=1 Tax=Ricinus communis RepID=B9RHV1_RICCO	-	-	6.0e-28	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	87%
Sma3	7-dehydrocholesterol reductase	-	-	3.745e-09	-

Source	ECs	Term	Type	e value	Identity
Sma3	7-dehydrocholesterol reductase.	EC:1.3.1.21	-	7.49e-09	-

Source	KEGGs	Term	e value	Identity
Sma3	Steroid biosynthesis	00100	7.49e-09	%
Sma3	Biosynthesis of terpenoids and steroids	01062	7.49e-09	%
Sma3	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	7.49e-09	%
Sma3	Biosynthesis of plant hormones	01070	7.49e-09	%
Sma3	Metabolic pathways	01100	7.49e-09	%
Sma3	Biosynthesis of secondary metabolites	01110	7.49e-09	%

Source	Gene names
Sma3	At1g50430; DWF5; GSVIVT00029627001; OSJNBa0035A24.50; Os02g0465400; OsI_07144; OsJ_06658; POPTRDRAFT_765023; POPTRDRAFT_833904; RCOM_1573850; ST7R;

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	sterol delta7 reductase activity	GO:0009918	Molecular Function	-
Sma3	7-dehydrocholesterol reductase activity	GO:0047598	Molecular Function	-
Sma3	cholesterol biosynthetic process	GO:0006695	Biological Process	-
Sma3	brassinosteroid biosynthetic process	GO:0016132	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Ergosterol biosynthesis ERG4/ERG24	IPR001171	-	-
Sma3	Protein of unknown function DUF1754, eukaryotic	IPR013865	-	-
Sma3	Sterol reductase, conserved site	IPR018083	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT1G50430.1	DWF5, PA, LE, ST7R, 7RED Ergosterol biosynthesis ERG4/ERG24 family chr1:18682175-18685555 REVERSE LENGTH=432	2.0e-32	62%
RefSeq	Arabidopsis thaliana	NP_001077693.1	7-dehydrocholesterol reductase [Arabidopsis thaliana]	2.0e-32	62%
RefSeq	Populus trichocarpa	XP_002316435.1	predicted protein [Populus trichocarpa]	3.0e-34	65%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LLV4

Fln msg: Unexpected stop codon in the beginning of your sequence, Distance to subject end: 310 aas, your sequence is shorter than subject: 96 - 462

Fln protein: SFV*RLFCIPQDGSASQFWDFCKEQGLRKP*RPWPKPTLIAWKLIASYAAFEAALQLLLPGERVTGPVSPAGNIPVYKANGVLAYFVTLTTYIAIW

Protein Length: 97

Fln nts: AGTTTTGTTTAACGTTTATTTTGTATACCACAGGATGGATCTGCATCTCAATTTTGGGATTTCTGCAAGGAGCAAGGTCTTAGGAAACCCTGAAGACCTTGGCCAAAACCAACTCTCATAGCATGGAAACTAATTGCATCATATGCAGCTTTTGAAGCAGCACTCCAACTACTTTTACCTGGTGAAAGAGTAACTGGACCTGTTTCTCCTGCAGGAAACATTCCAGTCTATAAGGCAAACGGTGTGCTGGCTTACTTTGTCACATTGACAACTTATATTGCTATCTGGTG

Fln Alignment:

AllPine_a_rep_c93391___DGSASQFWDFCKEQGLRKP*RPWPKPTLIAWKLIASYAAFEAALQLLLPGERVTGPVSPAGNIPVYKANGVLAYFVTLTTYIAIW
B8LLV4________________DGSASQFWDFCKQQGLQGFLRICPKPTLIAWELIASYAAFEAALQLLLPGERVIGPVSPAGNIPVYKANGVAAYFITLTTYIAIW

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain