SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene132533

Length: 386 nt

UniGene Fasta

>sp_v3.0_unigene132533
CGTCGTGACCTTGGCTGTCACTCAAGTGAATCGATAAAATGCATAAGCTCTTATAAATAGAACGTGACGCAGTCGGCCAAATTAAAAATGAAGTGACCCACATTCTCATCTATCTTTTTCTTGGTTCTGGCCTATGGTAATCGCAGCCGCCGCCGGAGGCGCAGGCAGTCATGGATCCTTCTCGTGCTTTTGTCAAGAATGCGAAGCGAGTTATAATCAAGGTTGGCACTGCTGTCGTCACTAGAGCTAATGGAGGGCTTGCCTTGGGTCGCCTTGGTTCCTTATGTGAGCAGGTTAAAGAACTGATGTCAGTCGGCCTTGAGGTTATACTTGTGACCTCAGGTGCAGTAGGGATTGGTCGGCAGAGGCTTCGCTACCAAGACTGA

Ace file of the UniGene sp_v3.0_unigene132533

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Pyrroline-5-carboxylate synthetase n=2 Tax=Euonymus japonicus RepID=B3V357_9ROSI	-	-	2.0e-18	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	98%
Sma3	Pyrroline-5-carboxylate synthetase	-	-	2.27e-30	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glutamate-5-semialdehyde dehydrogenase.	EC:1.2.1.41	-	4.782e-25	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Arginine and proline metabolism	00330		4.782e-25	%
Sma3	Metabolic pathways	01100		4.782e-25	%
Sma3	Glutamate 5-kinase.	EC:2.7.2.11	-	2.285e-32	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Arginine and proline metabolism	00330		2.285e-32	%
Sma3	Metabolic pathways	01100		2.285e-32	%

Source	Gene names
Sma3	At2g39800; At3g55610; F1I16_20; GSVIVT00018407001; GSVIVT00021385001; LOC_Os05g38150; OJ1651_D06.9; Os01g0848200; Os05g0455500; OsI_04432; OsI_20190; OsJ_04075; OsJ_18779; P0005H10.23; P5CS; P5CS-1; P5CS1; P5CS2; P5CSA; P5CSB; PHYPADRAFT_117839; POPTRDRAF

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	glutamate 5-kinase activity	GO:0004349	Molecular Function	-
Sma3	glutamate-5-semialdehyde dehydrogenase activity	GO:0004350	Molecular Function	-
Sma3	oxidoreductase activity	GO:0016491	Molecular Function	-
Sma3	proline biosynthetic process	GO:0006561	Biological Process	-
Sma3	response to oxidative stress	GO:0006979	Biological Process	-
Sma3	response to water deprivation	GO:0009414	Biological Process	-
Sma3	embryo development ending in seed dormancy	GO:0009793	Biological Process	-
Sma3	hyperosmotic salinity response	GO:0042538	Biological Process	-
Sma3	root development	GO:0048364	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Gamma-glutamyl phosphate reductase GPR	IPR000965	-	-
Sma3	Aspartate/glutamate/uridylate kinase	IPR001048	-	-
Sma3	Glutamate/acetylglutamate kinase	IPR001057	-	-
Sma3	Glutamate 5-kinase/delta-1-pyrroline-5-carboxylate synthase	IPR005715	-	-
Sma3	Delta l-pyrroline-5-carboxylate synthetase	IPR005766	-	-
Sma3	Aldehyde dehydrogenase domain	IPR015590	-	-
Sma3	Aldehyde dehydrogenase, N-terminal	IPR016162	-	-
Sma3	Aldehyde dehydrogenase, C-terminal	IPR016163	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT3G55610.1	P5CS2 delta 1-pyrroline-5-carboxylate synthase 2 chr3:20624278-20628989 REVERSE LENGTH=726	3.0e-17	68%
RefSeq	Arabidopsis thaliana	NP_001118846.1	Gamma-glutamyl phosphate reductase [Arabidopsis thaliana]	4.0e-17	68%
RefSeq	Populus trichocarpa	XP_002315202.1	predicted protein [Populus trichocarpa]	2.0e-19	73%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LR17

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 677 aas, your sequence is shorter than subject: 72 - 749

Fln protein: MDPSRAFVKNAKRVIIKVGTAVVTRANGGLALGRLGSLCEQVKELMSVGLEVILVTSGAVGIGRQRLRYQD*

Protein Length: 73

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGTGAATCGATAAAATGCATAAGCTCTTATAAATAGAACGTGACGCAGTCGGCCAAATTAAAAATGAAGTGACCCACATTCTCATCTATCTTTTTCTTGGTTCTGGCCTATGGTAATCGCAGCCGCCGCCGGAGGCGCAGGCAGTC_-_ATGGATCCTTCTCGTGCTTTTGTCAAGAATGCGAAGCGAGTTATAATCAAGGTTGGCACTGCTGTCGTCACTAGAGCTAATGGAGGGCTTGCCTTGGGTCGCCTTGGTTCCTTATGTGAGCAGGTTAAAGAACTGATGTCAGTCGGCCTTGAGGTTATACTTGTGACCTCAGGTGCAGTAGGGATTGGTCGGCAGAGGCTTCGCTACCAAGACTGA

Fln Alignment:

AllPine_a_rep_c87007___MDPSRAFVKNAKRVIIKVGTAVVTRANXXXXXXXXXXXCEQVKELMSVGLEVILVTSGAVGIGRQRLRYQ
B8LR17________________MDPSRAFVKNAKRVIIKVGTAVVTRANGGLALGRLGSLCEQVKELMSFGLEVILVTSGAVGIGRQRLRYQ

SNPs (tot: 2)

Position	A %	C %	G %	T %	Probability
192			66	33	0.997454
193			33	66	0.997457

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain