SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene130616

Length: 538 nt

UniGene Fasta

>sp_v3.0_unigene130616
ACACAATGCAACTGTTACTATTGTGCATTCTTACACCAAAAATGCTGCAGATATTACTCGGGAGGCAGATATTGTTGTTTCTGCCGCAGGCGTGGCAAATTTGGTTCGTGGTGACTGGCTGAAGCCTGGAGCTGTGGTGATTGATGTTGGCATAAATCCAGTCGAGGATCACAATAGCAAAAAGAGGTTACCGATTAGTTGGTGATGTTTGCTATGAGGAGGCCTCAAGAATAGTCTCAGCAATAACTCCTGTACCTGGTGGAGTTGGTCCAATGACAATTGCAATGCTTCTGTCCAATACTCTAGATTCAGCAAAACGAGCGTATGGCCTCCTTTAAGTTTTAGGTTCGATCTAATCCTTTAATAAGGGAGTTTCAATTTAAATTAATTCAAAATTCTTAAAGTTTCAGGAATGATGTTGAGCTGTGGTTCAGTAGAATTACAGCCTCGGGAGATATATTTTTGCAGTTTGAGGTCAACTTTCCCATAGGGTCACTTAAGCTTACATACCTTTGTATATGTAGTGGAAGTGCGTGTA

Ace file of the UniGene sp_v3.0_unigene130616

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Amino acid dehydrogenase family protein [Arabidopsis thaliana] gb\|AAC13627.1\| F6N23.26 gene product [Arabidopsis thaliana] emb\|CAB80871.1\| putative tetrahydrofolate synthase [Arabidopsis thaliana] gb\|AAL24426.1\| Unknown protein [Arabidopsis thaliana] gb\|A	-	-	1.0e-36	84%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	91%
Sma3	Tetrahydrofolate dehydrogenase/cyclohydrolase	-	-	4.351e-12	-

Source	ECs	Term	Type	e value	Identity
Sma3	Methylenetetrahydrofolate dehydrogenase (NADP(+)).	EC:1.5.1.5	-	2.399e-18	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	One carbon pool by folate	00670		2.399e-18	%
Sma3	Carbon fixation pathways in prokaryotes	00720		2.399e-18	%
Sma3	Metabolic pathways	01100		2.399e-18	%
Sma3	Methenyltetrahydrofolate cyclohydrolase.	EC:3.5.4.9	-	1.29e-22	-

Source	KEGGs	Term	e value	Identity
Sma3	One carbon pool by folate	00670	1.29e-22	%
Sma3	Carbon fixation pathways in prokaryotes	00720	1.29e-22	%
Sma3	Metabolic pathways	01100	1.29e-22	%

Source	Gene names
Sma3	AT4g00600; AT4g00620; At2g38660; At3g12290; At4g00600; At4g00620; At4g00620/F6N23.26; CHLREDRAFT_194856; F28J15.8; F6N23.26; F6N23.28; GSVIVT00004384001; GSVIVT00010817001; GSVIVT00026785001; GSVIVT00033609001; MICPUCDRAFT_49533; MICPUCDRAFT_57594; MICPUN

Source	GOs	Term	Type	Identity
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	nucleic acid binding	GO:0003676	Molecular Function	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	methenyltetrahydrofolate cyclohydrolase activity	GO:0004477	Molecular Function	-
Sma3	methylenetetrahydrofolate dehydrogenase (NADP+) activity	GO:0004488	Molecular Function	-
Sma3	binding	GO:0005488	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	ATP-dependent helicase activity	GO:0008026	Molecular Function	-
Sma3	hydrolase activity	GO:0016787	Molecular Function	-
Sma3	folic acid-containing compound biosynthetic process	GO:0009396	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Tetrahydrofolate dehydrogenase/cyclohydrolase	IPR000672	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3		IPR014021	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Aldehyde dehydrogenase, conserved site	IPR016160	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT4G00620.1	Amino acid dehydrogenase family protein chr4:259265-260788 REVERSE LENGTH=360	2.0e-38	84%
RefSeq	Arabidopsis thaliana	NP_191971.1	Amino acid dehydrogenase family protein [Arabidopsis thaliana]	2.0e-38	84%
RefSeq	Populus trichocarpa	XP_002326257.1	predicted protein [Populus trichocarpa]	3.0e-40	87%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NV85

Fln msg: Overlapping hits, possible frame ERROR between 184 and 177, your sequence is shorter than subject: 113 - 393

Fln protein: HNATVTIVHSYTKNAADITREADIVVSAAGVANLVRGDWLKPGAVVIDVGINPVEDHNxxxxGYRLVGDVCYEEASRIVSAITPVPGGVGPMTIAMLLSNTLDSAKRAYGLL*

Protein Length: 114

Fln nts: ACACAATGCAACTGTTACTATTGTGCATTCTTACACCAAAAATGCTGCAGATATTACTCGGGAGGCAGATATTGTTGTTTCTGCCGCAGGCGTGGCAAATTTGGTTCGTGGTGACTGGCTGAAGCCTGGAGCTGTGGTGATTGATGTTGGCATAAATCCAGTCGAGGATCACAATxxxxxxxxxxxxGGTTACCGATTAGTTGGTGATGTTTGCTATGAGGAGGCCTCAAGAATAGTCTCAGCAATAACTCCTGTACCTGGTGGAGTTGGTCCAATGACAATTGCAATGCTTCTGTCCAATACTCTAGATTCAGCAAAACGAGCGTATGGCCTCCTTTAAGTTTTAGGTTCGATCTAATCCTTTAATAAGGGAGTTTCAATTTAAATTAATTCAAAATTCTTAAAGTTTCAGGAATGATGTTGAGCTGTGGTTCAGTAGAATTACAGCCTCGGGAGATATATTTTTGCAGTTTGAGGTCAACTTTCCCATAGGGTCACTTAAGCTTACATACCTTTGTATATGTAGTGGAAGTGCGTGTA

Fln Alignment:

AllPine_a_rep_c83116___HNATVTIVHSYTKNAADITREADIVVSAAGVANLVRGDWLKPGAVVIDVGINPVEDHNxxxGYRLVGDVCYEEASRIVSAITPVPGGVGPMTIAMLLSNTLDSAKRAYGL
A9NV85________________HNATVTIVHSYTKNAEDITREADIVISAVGVANLVRGDWLKPGAVVIDVGINPVEDPNxxxGYRLVGDVCYEEASRIVSAITPVPGGVGPMTIAMLLSNTLESAKRAYGL

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
305			33	66	0.997452
306	66			33	0.997452
310		33		66	0.997452
312		66		33	0.997454
313	66		33		0.997452
322	33		66		0.997454
324			66	33	0.997458
326	33		66		0.997453

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain