SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene130070

Length: 406 nt

UniGene Fasta

>sp_v3.0_unigene130070
CGTCGTGACCTTGGCTGTCACTCAATTCTTTCCGCCAATTTTACAGGGAATGCGACATTTATGGAACCGTAGATTTCGTCTTGGGCTTGGCTGCTGTGGTGTTCCAGAACTGCAATCTTCTGGGCAGGATGCCGCTCGCCGGCCAGGGTATTGTCTATACAGCTCAAGGCAGGATAGACCCAAATCAGAATACTGGAACCTCTCTACACAATTGTACAGTCACTGCTGCGCCTGATCTCGTTCCGGTGGTGAGCTCTTTCCCAGTATACTTGGGGAGGCCATGGAAACTCTACTCTCGTACAGTTTATATGCAGTCTTTCTTAGACAGCTTGATCCAACCGGCAGGTTGGTCTGAATGGACGGGAACCTTTGCCTTGAGCACATTATATTATGGGGAATACATGAA

Ace file of the UniGene sp_v3.0_unigene130070

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Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=C0PT44_PICSI	-	-	70%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	70%
Sma3	Pectinesterase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Pectinesterase.	EC:3.1.1.11	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Pentose and glucuronate interconversions	00040	%
Sma3	Starch and sucrose metabolism	00500	%
Sma3	Metabolic pathways	01100	%

Source	Gene names
Sma3	ARATH1; ARATH12; ARATH13; ARATH16; ARATH17; ARATH20; ARATH21; ARATH22; ARATH23; ARATH24; ARATH25; ARATH27; ARATH28; ARATH32; ARATH33; ARATH34; ARATH35; ARATH36; ARATH38; ARATH39; ARATH4; ARATH40; ARATH41; ARATH44; ARATH45; ARATH46; ARATH47; ARATH54; ARATH

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	enzyme inhibitor activity	GO:0004857	Molecular Function	-
Sma3	pectinesterase activity	GO:0030599	Molecular Function	-
Sma3	aspartyl esterase activity	GO:0045330	Molecular Function	-
Sma3	fruit ripening	GO:0009835	Biological Process	-
Sma3	cell wall modification	GO:0042545	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Ribosome-binding factor A	IPR000238	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Metallophosphoesterase domain	IPR004843	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-
Sma3	Carbohydrate-binding/sugar hydrolysis domain	IPR006633	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G02810.1	Plant invertase/pectin methylesterase inhibitor superfamily chr1:618284-620333 FORWARD LENGTH=579	65%
RefSeq	Arabidopsis thaliana	NP_566379.1	pectinesterase 25 [Arabidopsis thaliana]	61%
RefSeq	Populus trichocarpa	XP_002302526.1	predicted protein [Populus trichocarpa]	70%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: C0PT44

Fln msg: Unexpected stop codon in the beginning of your sequence, Distance to subject end: 52 aas, your sequence is shorter than subject: 134 - 601

Fln protein: S*PWLSLNSFRQFYRECDIYGTVDFVLGLAAVVFQNCNLLGRMPLAGQGIVYTAQGRIDPNQNTGTSLHNCTVTAAPDLVPVVSSFPVYLGRPWKLYSRTVYMQSFLDSLIQPAGWSEWTGTFALSTLYYGEYM

Protein Length: 135

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTCTTTCCGCCAATTTTACAGGGAATGCGACATTTATGGAACCGTAGATTTCGTCTTGGGCTTGGCTGCTGTGGTGTTCCAGAACTGCAATCTTCTGGGCAGGATGCCGCTCGCCGGCCAGGGTATTGTCTATACAGCTCAAGGCAGGATAGACCCAAATCAGAATACTGGAACCTCTCTACACAATTGTACAGTCACTGCTGCGCCTGATCTCGTTCCGGTGGTGAGCTCTTTCCCAGTATACTTGGGGAGGCCATGGAAACTCTACTCTCGTACAGTTTATATGCAGTCTTTCTTAGACAGCTTGATCCAACCGGCAGGTTGGTCTGAATGGACGGGAACCTTTGCCTTGAGCACATTATATTATGGGGAATACATGAA

Fln Alignment:

AllPine_a_rep_c81831___LSLNSFRQFYRECDIYGTVDFVLGLAAVVFQNCNLLGRMPLAGQGIVYTAQGRIDPNQNTGTSLHNCTVTAAPDLVPVVSSFPVYLGRPWKLYSRTVYMQSFLDSLIQPAGWSEWTGTFALSTLYYGEYM
C0PT44________________LYVHSLRQFYRECNVYGTVDFIFGNAAVVLQNCNLFARKPLANQQIVYTAQGRQDPNENTGISIQNCQVIAASDLIPVKRSFPAYLGRPWRQYSRTVFMQSYLGDLIQPAGWLEWNGNFALNTLYYGEFM

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
78	50		50		1.0
81			50	50	1.0
83		50	50		1.0
84	50		50		1.0
85		50	50		1.0
87	50			50	1.0
88	50			50	1.0
89		50	50		1.0
92		50		50	1.0
96	50			50	1.0
97		50	50		1.0
99		50	50		1.0
100	50		50		1.0
139		25	75		0.996479
156		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain