SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene128321

Length: 371 nt

UniGene Fasta

>sp_v3.0_unigene128321
CGTCTTGGGGCTTCGCTTAGCCATTTCTGAGTTTGGCCATAGCGATTTTTTCCATTGGATCGTGATTTTGGATCAGTAAGTCCCCGAGGTGGCCTGGGATCGGTTCCTGGACTTCTTTGTCTTCAATTTCTTTCTGGAGCGATTTGTTTTGTTTTGTTCTGTTGTGGGTTTAGCTATCACAATGGCGTCGGAGAAGATCAAGGTGGAGAATCCCATCGTCGAGATGGACGGGGATGAAATGACCAGGGTCATATGGACAATGATTAAGGACAAGCTTATTTTCCATTTGTAGAGCTGGATATTAAGTATTTTGATTTGGGGCTTCCCCATCGCGATGCTACAGATGACAAGGTTACAATTGAGAGTGCAGA

Ace file of the UniGene sp_v3.0_unigene128321

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Isocitrate dehydrogenase [NADP] n=4 Tax=Pinaceae RepID=B8LKH5_PICSI	-	-	3.0e-24	97%
FL-Next	sp=Isocitrate dehydrogenase [NADP]; Pinus pinaster (Maritime pine).	-	-	0.0	100%
Sma3	NADP-dependent isocitrate dehydrogenase	-	-	6.211e-18	-

Source	ECs	Term	Type	e value	Identity
Sma3	Isocitrate dehydrogenase (NADP(+)).	EC:1.1.1.42	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Citrate cycle (TCA cycle)	00020	%
Sma3	Glutathione metabolism	00480	%
Sma3	Carbon fixation pathways in prokaryotes	00720	%
Sma3	Biosynthesis of phenylpropanoids	01061	%
Sma3	Biosynthesis of terpenoids and steroids	01062	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	%
Sma3	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	%
Sma3	Biosynthesis of alkaloids derived from histidine and purine	01065	%
Sma3	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	%
Sma3	Biosynthesis of plant hormones	01070	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	At1g54340; At1g65930; At5g14590; CHLREDRAFT_196567; DcNADP-ICDH1; DcNADP-ICDH2; EgICDH; F20D21.16; GSVIVT00002132001; GSVIVT00023479001; ICDH; ICDH-1; ICDH-2; IDH; IDH1; IDH3; Icdh; LaICDH1; LaICDH2; OJ1735_C10.15; OSIGBa0101P20.6; OSJNBa0043L24.16; OSJNB

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	peroxisome	GO:0005777	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	magnesium ion binding	GO:0000287	Molecular Function	-
Sma3	isocitrate dehydrogenase (NAD+) activity	GO:0004449	Molecular Function	-
Sma3	isocitrate dehydrogenase (NADP+) activity	GO:0004450	Molecular Function	-
Sma3	copper ion binding	GO:0005507	Molecular Function	-
Sma3	manganese ion binding	GO:0030145	Molecular Function	-
Sma3	NAD binding	GO:0051287	Molecular Function	-
Sma3	glyoxylate cycle	GO:0006097	Biological Process	-
Sma3	tricarboxylic acid cycle	GO:0006099	Biological Process	-
Sma3	isocitrate metabolic process	GO:0006102	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Isocitrate/isopropylmalate dehydrogenase	IPR001804	-	0.0	-
Sma3	Isocitrate dehydrogenase NADP-dependent, eukaryotic-type	IPR004790	-	0.0	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT1G65930.1	cICDH cytosolic NADP+-dependent isocitrate dehydrogenase chr1:24539088-24541861 FORWARD LENGTH=410	6.0e-23	100%
RefSeq	Arabidopsis thaliana	NP_176768.1	isocitrate dehydrogenase [Arabidopsis thaliana]	8.0e-23	100%
RefSeq	Populus trichocarpa	XP_002305928.1	predicted protein [Populus trichocarpa]	1.0e-23	96%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A0AR16

Fln msg: Overlapping hits, possible frame ERROR between 283 and 280, Distance to subject end: 353 aas, your sequence is shorter than subject: 63 - 417

Fln protein: MASEKIKVENPIVEMDGDEMTRVIWTMIKDKLxxxFVELDIKYFDLGLPHRDATDDKVTIESA

Protein Length: 64

Fln nts: CGTCTTGGGGCTTCGCTTAGCCATTTCTGAGTTTGGCCATAGCGATTTTTTCCATTGGATCGTGATTTTGGATCAGTAAGTCCCCGAGGTGGCCTGGGATCGGTTCCTGGACTTCTTTGTCTTCAATTTCTTTCTGGAGCGATTTGTTTTGTTTTGTTCTGTTGTGGGTTTAGCTATCACA_-_ATGGCGTCGGAGAAGATCAAGGTGGAGAATCCCATCGTCGAGATGGACGGGGATGAAATGACCAGGGTCATATGGACAATGATTAAGGACAAGCTTxxxxxxxxxTTTGTAGAGCTGGATATTAAGTATTTTGATTTGGGGCTTCCCCATCGCGATGCTACAGATGACAAGGTTACAATTGAGAGTGCAGA

Fln Alignment:

AllPine_a_rep_c76697___MASEKIKVENPIVEMDGDEMTRVIWTMIKDKLIxPFVELDIKYFDLGLPHRDATDDKVTIESA
A0AR16________________MASEKIKVENPIVEMDGDEMTRVIWTMIKDKLIxPFVELDIKYFDLGLPHRDATDDKVTIESA

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
121	22	77			0.999999
122	22			77	0.999999
124		77	22		0.999999
125	75			25	1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
143	158	TTTGT TTTGT TTTGT T	16

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain