SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene127866

Length: 1493 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene127866

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cytosolic monodehydroascorbate reductase n=5 Tax=Poaceae RepID=Q9XFZ3_ORYSJ	-	-	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Sma3	Monodehydroascorbate reductase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Monodehydroascorbate reductase (NADH).	EC:1.6.5.4	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Ascorbate and aldarate metabolism	00053		0.0	%
Sma3	Metabolic pathways	01100		0.0	%

Source	Gene names
Sma3	49D11.20a; 49D11.20b; AFRR; AT3G52880; At1g63940; At3g09940; At3g27820; At3g52880; At5g03630; BO-MDAR 1; BO-MDAR 2; CHLREDRAFT_139735; F17C15_50; F8J2_50; GSVIVT00002544001; GSVIVT00020720001; GSVIVT00021644001; GSVIVT00027404001; K16N12.2; MDAR; MDAR1; M

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	peroxisomal membrane	GO:0005778	Cellular Component	-
Sma3	peroxisomal matrix	GO:0005782	Cellular Component	-
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	cysteine-type peptidase activity	GO:0008234	Molecular Function	-
Sma3	oxidoreductase activity	GO:0016491	Molecular Function	-
Sma3	monodehydroascorbate reductase (NADH) activity	GO:0016656	Molecular Function	-
Sma3	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	-
Sma3	proteolysis	GO:0006508	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	response to cold	GO:0009409	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	response to jasmonic acid stimulus	GO:0009753	Biological Process	-
Sma3	hydrogen peroxide catabolic process	GO:0042744	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Pyridine nucleotide-disulphide oxidoreductase, class-II	IPR000103	-	-
Sma3	Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain	IPR001327	-	-
Sma3	Peptidase C48, SUMO/Sentrin/Ubl1	IPR003653	-	-
Sma3	Protein BYPASS-related	IPR008511	-	-
Sma3	Nucleic acid-binding, OB-fold	IPR012340	-	-
Sma3	FAD-dependent pyridine nucleotide-disulphide oxidoreductase	IPR013027	-	-
Sma3	Replication factor A, C-terminal	IPR013955	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G52880.2	ATMDAR1, MDAR1 monodehydroascorbate reductase 1 chr3:19601477-19604366 REVERSE LENGTH=466	69%
RefSeq	Arabidopsis thaliana	NP_001154674.1	monodehydroascorbate reductase (NADH) [Arabidopsis thaliana]	69%
RefSeq	Populus trichocarpa	XP_002326252.1	predicted protein [Populus trichocarpa]	71%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NUH7

Fln msg: Overlapping hits, possible frame ERROR between 600 and 585, your sequence is shorter than subject: 414 - 434

Fln protein: KLGVKSGELAIISKEAVAPYERPALSKAYLFPQGAVRLPSFHVCVGSGGERQLPEWYTEKGIELILGTEVVKAEIATKTLTSAEGTTFKYDTLIIATGSTVIKLSEFGVQGADAKGIFYLREIAEADALVSVIQSKPEGTAVVVGGGYIGLELAAVLKINKYKVKLVCPEPGFMPRLFTADIASFYEGYYKGKTxxxxxxxATGFSTDEHGNVKMVKLKNGRELEADIVVVGVGARPLTGLFKGQLQEEKGGIKTDGSFRTSVPDVYAVGDVAAFPMKIYGDIRRVEHVDHARKSAAQAVQAIKAKEEGRIVEEYDYLPYFYSRSFDLSWQFYGDNVGESVIFGDNNPATSKAKFGSYWIKDGRVVGVFLESGTPEENKAIAKVARVKPLVESKEELIKDGLAFASKISSAFT*

Protein Length: 415

Fln nts: AAGTTGGGTGTCAAATCTGGAGAGCTCGCGATTATATCAAAAGAGGCTGTAGCTCCATATGAAAGACCTGCACTTAGCAAAGCTTATCTCTTTCCGCAAGGAGCTGTGCGACTTCCGAGCTTCCATGTCTGTGTTGGGAGTGGAGGAGAGAGGCAGCTTCCGGAATGGTATACTGAGAAAGGTATAGAGTTGATTCTTGGTACTGAAGTGGTGAAAGCTGAAATTGCCACCAAAACTCTCACTAGCGCAGAAGGGACTACTTTCAAATATGACACTTTAATCATTGCAACTGGTTCCACTGTTATCAAGTTATCTGAGTTCGGCGTGCAAGGAGCTGATGCCAAGGGCATCTTTTATCTGAGAGAGATTGCTGAAGCTGATGCCTTGGTATCTGTAATACAGTCAAAACCAGAGGGCACAGCTGTGGTTGTTGGTGGAGGCTATATTGGGCTTGAACTTGCTGCAGTCCTAAAAATCAACAAATATAAAGTTAAATTGGTGTGCCCCGAGCCAGGGTTCATGCCTCGTCTTTTCACTGCAGACATAGCTTCTTTCTATGAGGGCTATTATAAAGGAAAGACTxxxxxxxxxxxxxxxxxxxxxGCCACAGGATTCAGCACTGATGAGCATGGAAATGTTAAGATGGTAAAGCTGAAGAATGGCAGGGAACTGGAGGCAGATATTGTTGTTGTTGGTGTTGGAGCAAGACCTTTGACAGGCCTTTTCAAAGGTCAGCTTCAGGAGGAAAAGGGTGGCATTAAGACGGATGGTTCCTTCAGAACTAGTGTTCCTGATGTTTATGCGGTAGGAGACGTGGCAGCTTTCCCAATGAAGATTTACGGTGACATTAGAAGAGTCGAACATGTTGATCATGCTAGGAAATCAGCAGCACAAGCCGTGCAGGCAATTAAAGCCAAAGAAGAAGGAAGGATCGTTGAAGAATATGACTATCTTCCATATTTTTATTCACGTTCATTTGATTTATCATGGCAATTCTATGGAGACAATGTAGGTGAATCCGTTATTTTCGGTGACAATAATCCTGCGACATCGAAAGCCAAATTTGGATCATATTGGATAAAAGATGGTAGGGTGGTAGGTGTATTCTTGGAGAGTGGTACTCCTGAAGAAAACAAAGCTATTGCTAAGGTAGCACGTGTGAAGCCTTTGGTTGAAAGCAAGGAGGAACTGATAAAGGATGGGCTGGCATTTGCTTCTAAGATATCCTCGGCTTTCACTTAAATCGGCTTTCTGTTAATTCATCAGCATGCTGCATTACTGTTGGTCTCTGTTCACCTTGGATATATCTGTTGTTTTAGCGTCTGATTGACTTTTGTTATGAGATATTGGATTTGACTTTTGTTATGAGGCATTGGACTTTTAGTCCGGGTGTAGCTACTTCGTTAGTAAATCAAACTCGCTACATATTTTTGCTCTTTTTTTCCTACTGCTGGGATGGCATCTTATTAAACAAGACTATATTATTATTTCGAT

Fln Alignment:

AllPine_a_rep_c73876___KLGVKSGELAIISKEAVAPYERPALSKAYLFPQGAVRLPSFHVCVGSGGERQLPEWYTEKGIELILGTEVVKAEIATKTLTSAEGTTFKYDTLIIATGSTVIKLSEFGVQGADAKGIFYLREIAEADALVSVIQSKPEGTAVVVGGGYIGLELAAVLKINKYKVKLVCPEPGFMPRLFTADIASFYExxxxxxxxxxxxx--ATGFSTDEHGNVKMVKLKNGRELEADIVVVGVGARPLTGLFKGQLQEEKGGIKTDGSFRTSVPDVYAVGDVAAFPMKIYGDIRRVEHVDHARKSAAQAVQAIKAKEEGRIVEEYDYLPYFYSRSFDLSWQFYGDNVGESVIFGDNNPATSKAKFGSYWIKDGRVVGVFLESGTPEENKAIAKVARVKPLVESKEELIKDGLAFASKI
A9NUH7________________KLGLKSGELAIISKEAVAPYERPALSKAYLFPEGAARLPGFHVCVGIGGERQLPEWYSEKGIELILSTEVVKAEIASKTLTTAEGTTFKYDTLIIATGSTVIKLSDFGVQGADAKGIFYLREIAEADALVSAIQSKPEGTAVIVGGGYIGLELAAVLRINKYKVKMVYPEPWCMPRLFTADIAAFYExxxxxxxxxxxxxTVATGFVTDEHENVKIVKLKDGRELEADIVVVGVGGRPLTGLFKGQLEEEKGGIKTDGFFKTSVPDVYAVGDVATFPMKIYGDIRRVEHVDHARKSAAQAVQAIKAKEEGKSFEEYDYLPYFYSRSFDLSWQFYGDNVGEAIIFGDNNPATPKAKFGSYWIKDGRVVGVFLENGTPEENKAIAKVARLKPLVESKEELIKDGLAFASKI

SNPs (tot: 3)

Position	A %	C %	G %	T %	Probability
1110		20		80	0.995493
1114	16	83			0.994512
1117			16	83	0.994512

SSRs (tot: 1)

Start position	End position	Sequence	Length
682	693	TTG TTG TTG TTG	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain