SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene126978

Length: 1108 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene126978 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Glycolate oxidase n=1 Tax=Zantedeschia aethiopica RepID=Q84LB8_ZANAE	-	-	0.0	80%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	85%
Sma3	Glycolate oxidase	-	-	1.898e-29	-

Source	ECs	Term	Type	e value	Identity
Sma3	Transferred entry: 1.1.3.15.	EC:1.1.3.1	-	5.855e-08	-
Sma3	(S)-2-hydroxy-acid oxidase.	EC:1.1.3.15	-	7.82e-28	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Glyoxylate and dicarboxylate metabolism	00630		7.82e-28	%
Sma3	Metabolic pathways	01100		7.82e-28	%

Source	Gene names
Sma3	AT4g18360; At3g14130; At3g14415; At3g14420; At4g18360; B1056G08.112; B1364A02.33-1; F28J12.20; GLO; GOX; GSVIVT00000984001; GSVIVT00028321001; GSVIVT00034039001; GSVIVT00034041001; Gox; H0215F08.7; H0215F08.8; LOC_Os03g57220; LeGLO2; MAO2.2; MLN21.20; OSJ

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	peroxisome	GO:0005777	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	(S)-2-hydroxy-acid oxidase activity	GO:0003973	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	FMN binding	GO:0010181	Molecular Function	-
Sma3	oxidoreductase activity	GO:0016491	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	oxidative photosynthetic carbon pathway	GO:0009854	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	FMN-dependent dehydrogenase	IPR000262	-	-
Sma3	FMN-dependent alpha-hydroxy acid dehydrogenase, active site	IPR008259	-	-
Sma3	Alpha-hydroxy acid dehydrogenase, FMN-dependent	IPR012133	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-
Sma3		IPR017934	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G18360.1	Aldolase-type TIM barrel family protein chr4:10146141-10148386 REVERSE LENGTH=368	85%
RefSeq	Arabidopsis thaliana	NP_193570.1	(S)-2-hydroxy-acid oxidase [Arabidopsis thaliana]	85%
RefSeq	Populus trichocarpa	XP_002317470.1	predicted protein [Populus trichocarpa]	88%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NLU2

Fln msg: Overlapping hits, possible frame ERROR between 692 and 629, and overlapping frame ERROR between 901 and 898,

Fln protein: MEIVNVSDYEAVAKEKLPKMVFDYYASGAEDQWTLQENRKAFERIRFRPRILVDVTKVDLSTTVLGFKISMPIMIAPTAMQKMAHPEGEFATARAASAAGTIMTLSSWATSSVEEVASTGPGIRxxxxxxxxxxxxxxxxxxxxxxxGFKAIALTVDTPRLGRRESDIKNRFALPPHLTLKNFEGLDLGASGIFIGRPVVFSLAAEGEAGVRNxxxxSRNEFELTMALAGCCSVKEINRNYIQTEADMIRSISRL*

Protein Length: 257

Fln nts: ATGGGGGATCTTATCTGCGGTGCCTGTCACATGGGGATGGTTGACAGAATCGCCATTTCCCGGAGCTGGCGATAATAGAGAGCCAACTAAGTAGCCGTCGTAGCAGGGCTTCGCAGAATTCCTCCCCGCGGAGCTAAGATATGATGCCTTTCATGGCTGCTTAGCACCTATTTAAATAGAGCGTTGTGTGATCCAAATCTTGAAGCCTGCTGCTCAACCAAAGATTGGATAGTATCTGTAATTAGAGTTAAGAT_-_ATGGAGATCGTGAACGTTTCCGACTATGAGGCAGTAGCCAAGGAAAAGCTGCCCAAAATGGTATTCGATTACTATGCTTCCGGTGCTGAAGATCAATGGACTCTCCAAGAAAATCGCAAAGCTTTCGAAAGAATAAGGTTCCGTCCCCGTATATTGGTTGATGTAACAAAGGTGGATCTGAGTACCACTGTGTTGGGATTCAAGATATCAATGCCTATTATGATTGCTCCAACTGCCATGCAAAAGATGGCTCATCCCGAAGGAGAGTTTGCTACTGCAAGAGCTGCGTCAGCTGCTGGAACTATAATGACGCTTTCTTCATGGGCTACATCAAGTGTTGAAGAGGTTGCTTCAACTGGACCAGGCATTCGCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGGTTTTAAGGCAATTGCACTGACTGTGGACACACCTCGACTTGGGCGGAGGGAGTCTGACATTAAAAATAGATTCGCATTGCCTCCACATCTTACTTTAAAGAATTTCGAGGGTTTGGATCTTGGAGCCTCTGGCATATTTATTGGGAGGCCTGTAGTTTTTTCTTTGGCTGCAGAGGGAGAAGCAGGGGTGAGAAATGAxxxxxxxxxGTCACGCAATGAGTTTGAACTAACCATGGCCTTAGCAGGATGCTGTTCAGTGAAGGAGATAAATCGAAATTACATTCAAACTGAGGCCGACATGATTCGATCTATATCAAGGCTCTGA___AAGGCTCTGCTCTCACATGAGTGATTGTTGACCAACAAAGTGAATATGCAATTGCTTGTGCCTTTTTAATTGATATGCTACTTATGCA

Fln Alignment:

AllPine_a_rep_c67521___MEIVNVSDYEAVAKEKLPKMVFDYYASGAEDQWTLQENRKAFERIRFRPRILVDVTKVDLSTTVLGFKISMPIMIAPTAMQKMAHPEGEFXXXXXXXXAGTIMTLSSWATSSVEEVASTGPGIRFxxxxxxxxxxxxxxxxxxxxxxxFKAIALTVDTPRLGRRESDIKNRFALPPHLTLKNFEGLDLGASGIFIGRPVVFSLAAEGEAGVRNDLxMSRNEFELTMALAGCCSVKEINRNYIQTEADMIRSISR
A9NLU2________________MEIVNVSDYEVVAKQKLPKMVFDYYASGAEDQWTLHENRKAFERIRFRPRILIDVTKVDLSTTVLGFKISMPIMIAPTAMQKMAHPEGEFATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFxxxxxxxxxxxxxxxxxxxxxxxFKAIALTVDTPRLGRREA-------------------LALGASGIFIGRPVVFSLAAEGEAGVRNVLxMLRDEFELTMALAGCCSVKEINRNYIQTEADMIRSISR

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
683	16	83			0.994516
685		83		16	0.994517
686		83	16		0.994517
707	83		16		0.994512
1070		14		85	0.993533
1072	71	14			0.984337
1074		85		14	0.993533
1075		85	14		0.993533
1077			14	85	0.993533
1079	25	25			0.995595
1080		75		25	0.99649
1081		75		25	0.99649

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain