SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene126719

Length: 1538 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene126719

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase n=10 Tax=rosids RepID=METE_ARATH	-	-	0.0	89%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	96%
Sma3	Cobalamin-independent methionine synthase	-	-	1.623e-23	-

Source	ECs	Term	Type	e value	Identity
Sma3	5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase.	EC:2.1.1.14	-	2.074e-39	-

Source	KEGGs	Term	e value	Identity
Sma3	Cysteine and methionine metabolism	00270	2.074e-39	%
Sma3	Selenocompound metabolism	00450	2.074e-39	%
Sma3	Biosynthesis of plant hormones	01070	2.074e-39	%
Sma3	Metabolic pathways	01100	2.074e-39	%
Sma3	Biosynthesis of secondary metabolites	01110	2.074e-39	%

Source	Gene names
Sma3	At3g03780; At5g17920; At5g20980; BvMS1; CHLREDRAFT_154307; CIMS; ER69; GSVIVT00003836001; GSVIVT00004893001; GSVIVT00017439001; GSVIVT00031893001; HvMS; LOC_Os12g42884; MET; METE; MPI7.9; MS1; MS2; MS3; MS4; MS5; Os12g0623900; Os12g0624000; OsI_39179; OsI

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	peroxisome	GO:0005777	Cellular Component	-
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	ribosome	GO:0005840	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	structural constituent of ribosome	GO:0003735	Molecular Function	-
Sma3	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity	GO:0003871	Molecular Function	-
Sma3	copper ion binding	GO:0005507	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	methionine synthase activity	GO:0008705	Molecular Function	-
Sma3	translation	GO:0006412	Biological Process	-
Sma3	cellular amino acid biosynthetic process	GO:0008652	Biological Process	-
Sma3	methionine biosynthetic process	GO:0009086	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	cellular protein metabolic process	GO:0044267	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Ribosomal protein S7e	IPR000554	-	-
Sma3	Chaperonin Cpn60/TCP-1	IPR002423	-	-
Sma3	Methionine synthase, vitamin-B12 independent	IPR002629	-	-
Sma3	Cobalamin-independent methionine synthase	IPR006276	-	-
Sma3	Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal	IPR013215	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G17920.1	ATCIMS, ATMETS, ATMS1 Cobalamin-independent synthase family protein chr5:5935771-5939195 FORWARD LENGTH=765	89%
RefSeq	Arabidopsis thaliana	NP_001078599.1	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Arabidopsis thaliana]	89%
RefSeq	Populus trichocarpa	XP_002325856.1	vitamin-b12 independent methionine synthase, 5-methyltetrahydropteroyltriglutamate-homocysteine [Populus trichocarpa]	87%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LPY2

Fln msg: your sequence is shorter than subject: 388 - 766

Fln protein: NVASALASRRTSPRVTNEAVQKAAAALKTSDHRRATPVNIRLEQQQKKLNLPILPTTTIGSFPQTVELRRVRREFKINKISEEEYVSSIKEEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPVIYGDVSRPKAMTVFWSKMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRSETCMQIALAIKDEVEDLEAGGIQVIQIDEAALREGLPLRKAEHAYYLDWAVHGFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSVEEIADRISKMLAVLETNILWVNPDCGLKTRKYTEVIPALTNVVAAAKLLRAQLASAK*

Protein Length: 389

Fln nts: AATGTCGCATCTGCACTGGCTTCAAGGAGGACGTCTCCAAGAGTGACAAACGAGGCAGTTCAAAAAGCTGCTGCAGCGTTGAAGACTTCTGACCATCGACGAGCTACGCCAGTAAACATTCGTCTTGAGCAACAACAGAAAAAGTTGAATCTTCCAATTCTTCCCACGACAACAATTGGGTCATTCCCACAGACAGTTGAATTGCGTAGGGTTCGTCGTGAATTCAAGATAAACAAGATTTCAGAGGAAGAATATGTTAGCTCCATCAAGGAAGAAATTGCCAAGGTTGTCAAATTGCAAGAGGACCTTGATATTGATGTGCTTGTGCACGGAGAACCCGAGAGGAATGACATGGTGGAGTACTTTGGAGAACAGCTTCAGGGCTTTGCATTTACAGTCAATGGTTGGGTACAGTCTTATGGATCCCGTTGTGTGAAGCCACCAGTTATCTATGGTGATGTCAGTCGCCCAAAGGCTATGACTGTCTTTTGGTCCAAGATGGCTCAGAGCATGACTGCTCGACCCATGAAGGGAATGCTCACTGGCCCAGTGACAATTTTGAACTGGTCCTTCGTTAGAAATGATCAACCAAGATCTGAAACTTGCATGCAAATTGCCTTGGCCATCAAGGATGAGGTGGAAGACTTGGAAGCGGGTGGAATTCAGGTTATTCAAATTGACGAGGCTGCTCTTAGAGAAGGATTGCCTCTACGCAAGGCTGAACATGCTTATTACCTGGATTGGGCTGTACACGGATTCCGCATCACTAACTGTGGTGTGCAAGATACAACTCAGATCCATACCCACATGTGCTACTCTAACTTCAACGATATTATTCATTCAATCATTGATATGGATGCCGATGTAATCACCATTGAGAATTCTCGTTCCGATGAGAAACTTCTCTCAGTTTTCCGAGAGGGTGTGAAATATGGTGCCGGAATTGGCCCTGGTGTCTATGACATTCACTCTCCTCGCATTCCAAGCGTAGAAGAAATTGCAGATCGTATCAGCAAGATGCTTGCTGTGCTCGAAACAAACATACTATGGGTTAACCCTGACTGTGGTCTGAAGACCAGGAAGTACACCGAGGTCATTCCTGCCCTGACCAACGTGGTTGCAGCTGCCAAATTGCTGCGAGCTCAGTTGGCCAGTGCAAAGTGA___GGCCATCCCAGCTATCTTCAAGTACTATGAGTGTTGGAATTTTTTTGTGGTTCTTTTCATAAATAACTTTTGTGTTTCTTATTTTCATTCTCATAAGTAGTTGATGTGGCTGAGTGCTCTCAAGGCAGGCTTTGGAGCCACATTTCTCCATCCAGAATATTTTCTTATTTACTAGTGAATATTAATTGAGTAAACCCTCAAAAGTGTCGGTGCCTCCATTCTCTTTGAAAGGCGAGTTATTAAATGAAGTTTTTCTATGAAACATTGGACGAGATTTGATGATGTGATGCTTCCTCTGATTGTTTGAATCAGTATTCCATTTAAAGCATGTTATCTATCATATTAAGTATTCCATTTAAAGCATGTTATCTGTC

Fln Alignment:

AllPine_a_rep_c65382___ASALASRRTSPRVTNEAVQKAAAALKTSDHRRATPVNIRLEQQQKKLNLPILPTTTIGSFPQTVELRRVRREFKINKISEEEYVSSIKEEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPVIYGDVSRPKAMTVFWSKMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRSETCMQIALAIKDEVEDLEAGGIQVIQIDEAALREGLPLRKAEHAYYLDWAVHGFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSVEEIADRISKMLAVLETNILWVNPDCGLKTRKYTEVIPALTNVVAAAKLLRAQLASAK
B8LPY2________________AAALASRRASPRVTNEAVQKAAAALKTSDHRRVTPVNVRLEQQQKKLNLPILPTTTIGSFPQTVELRRVRREYKINKISEDEYVSSIKEEIAKVVKLQEDLDIDVLVHGEPERNDMVEYFGEQLQGFAFTVNGWVQSYGSRCVKPPVIYGDVSRPKPMTVFWSKVAQSMTARPMKGMLTGPVTILNWSFVRNDQPRSETCMQIALAIKDEVEDLEAGGIQVIQIDEAALREGLPLRKAEHAYYLDWAVHGFRITNSGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRISKMLAVLETNILWVNPDCGLKTRKYPEVVPALTNMVAAAKLLRAQLASAK

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
586		93	2		0.983471
587	95		4		0.996599
588	95		4		0.996599
590		95	4		0.996599
747		97		2	0.967822
784		97		2	0.964186
949		1		94	0.936582
1023	5	95			0.997749
1026	94			5	0.997984
1027			94	5	0.998397
1502		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain