SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene111136

Length: 982 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene111136

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cytochrome P450 750A1 n=1 Tax=Pinus taeda RepID=C75A1_PINTA	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	68%
Sma3	Flavonoid 3'-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:1.14.-.-	-	1.646e-18	-
Sma3	Flavonoid 3'-monooxygenase.	EC:1.14.13.21	-	1.005e-09	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Flavonoid biosynthesis	00941		1.005e-09	%
Sma3	Flavone and flavonol biosynthesis	00944		1.005e-09	%
Sma3	Biosynthesis of phenylpropanoids	01061		1.005e-09	%
Sma3	Metabolic pathways	01100		1.005e-09	%
Sma3	Biosynthesis of secondary metabolites	01110		1.005e-09	%
Sma3	4-hydroxyphenylacetaldehyde oxime monooxygenase.	EC:1.14.13.68	-	1.051e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Cyanoamino acid metabolism	00460		1.051e-07	%
Sma3	Biosynthesis of secondary metabolites	01110		1.051e-07	%
Sma3	Flavonoid 3',5'-hydroxylase.	EC:1.14.13.88	-	6.369e-12	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	6.369e-12	%
Sma3	Flavone and flavonol biosynthesis	00944	6.369e-12	%
Sma3	Biosynthesis of secondary metabolites	01110	6.369e-12	%

Source	Gene names
Sma3	AT4g12300; AT4g12310; AT4g12320; At2g45550; At2g45570; At3g48280; At3g48300; At3g48320; At4g12300; At4g12300/T4C9_140; At4g12310; At4g12320; At5g07990; CYP71A1; CYP71A21; CYP71A23; CYP71A25; CYP71A8; CYP71AP2v1; CYP71AP3; CYP71D10; CYP736A3v2; CYP736A4v1;

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	flavonoid 3'-monooxygenase activity	GO:0016711	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3		GO:0050381	Molecular Function	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3	fruit ripening	GO:0009835	Biological Process	-
Sma3	DNA integration	GO:0015074	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protease inhibitor I4, serpin	IPR000215	-	-
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G12310.1	CYP706A5 cytochrome P450, family 706, subfamily A, polypeptide 5 chr4:7310598-7312522 REVERSE LENGTH=520	45%
RefSeq	Arabidopsis thaliana	NP_192968.3	cytochrome P450, family 706, subfamily A, polypeptide 5 [Arabidopsis thaliana]	45%
RefSeq	Populus trichocarpa	XP_002310011.1	predicted protein [Populus trichocarpa]	49%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AA14

Fln msg: Overlapping hits, possible frame ERROR between 631 and 613, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 248 - 559

Fln protein: *G*AC*RLR*CAPRYGS*DR*GGEQNYTRKIKAILLVMYIGGVGNTSNTLEWAMSELLRHPHVMKTLKEEIESVVGQHWKVKETDLASMKYLYCVVKETVRLYPGAPLGFPHASVEAMTVGGYHIPKKSMLMFNLWAIGRDPNVWGTDSSEFKPERFMQEESVDLTGRPSDFRMLPFGAGRRGCPGVVMATSMIELALAQLLHxxxxxxxxDPSEVDMKEAHSAALARQVPLFAFLTLRKPSCLSTV*

Protein Length: 249

Fln nts: ATGAGGCTGAGCCTGTTAAAGACTTCGTTGATGTGCTCCTCGATATGGCAGCTGAGATCGATAAGGCGGAGAACAAAATTACACGAGAAAAATCAAGGCAATCTTACTGGTGATGTATATCGGAGGAGTAGGGAATACATCCAATACGTTAGAGTGGGCAATGAGTGAGCTGCTCAGACATCCCCATGTAATGAAGACATTGAAAGAAGAAATCGAATCGGTCGTTGGCCAACACTGGAAAGTAAAGGAGACGGATCTGGCCAGCATGAAATACCTATACTGCGTGGTGAAAGAGACCGTACGATTATACCCAGGGGCACCTCTAGGGTTTCCCCATGCGTCTGTGGAGGCAATGACAGTAGGAGGATACCATATACCTAAGAAATCAATGCTAATGTTCAACTTGTGGGCAATTGGAAGGGATCCCAATGTGTGGGGAACAGATTCTTCAGAATTCAAGCCTGAAAGATTTATGCAGGAAGAGTCTGTAGATTTAACAGGACGTCCATCAGACTTCAGAATGCTTCCATTCGGGGCAGGAAGGAGAGGATGCCCGGGAGTTGTAATGGCTACTTCGATGATTGAGCTTGCCTTGGCTCAACTGTTGCACxxxxxxxxxxxxxxxxxxxxxxxxGATCCGTCCGAGGTGGACATGAAGGAGGCACATTCAGCGGCGTTGGCGAGACAAGTGCCACTCTTTGCTTTTCTGACGTTGAGGAAACCCAGTTGCCTCTCGACTGTCTAGATGGCACGGAAGCTGGCCAAAAGACCTGCTGTGGAACTCGTCATTGGCCATCTATCTTCAGTATAAGTAGAATAAAATTGTCGTTGGTAAATATTCTATAATAATGACAGGGTTACAGTCATGGTATTGTAGAGATAATGTTTGATGATGTCTTCAGGTAAAGATGTCTCTGTCTGATATTTGTGTGGGAGTCTTTCTTTGTACTGATTATATATAATTTCATAATTGATAAGCTCTA

Fln Alignment:

AllPine_a_c44815___EQNYTR-KIKAILLVMYIGGVGNTSNTLEWAMSELLRHPHVMKTLKEEIESVVGQHWKVKETDLASMKYLYCVVKETVRLYPGAPLGFPHASVEAMTVGGYHIPKKSMLMFNLWAIGRDPNVWGTDSSEFKPERFMQEESVDLTGRPSDFRMLPFGAGRRGCPGVVMATSMIELALAQLLHVxxxxxxGDPSEVDMKEAHSAALARQVPLFAFLTLRKPSC
D5AA14________________ETKITREKIKAIVLDIFGGGAAGTFTTIEWAMSELLRHPHTMKRLQEEIESVIGKHLKVNESDLVSMKYLQCVVKETLRLYPAGPLTLPRESVEAVTIAGYYIPKKTLLMVNLWAIGRDPNLWGADASEFKPERFMKEQYIDLIGQ-SDFKMLPFGAGRRGCPGYPMAIPIVELALAQLLHVxxxxxxGDPSQLDMKEACGASMPRQVPLSSSPSLRLPTC

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
605	25	75			0.996479
608			25	75	0.996475
609			25	75	0.996475
610	50		50		1.0
619	25			75	0.996476
620	25		25		0.995544
653	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain