SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene100935

Length: 1177 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene100935

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Threonine synthase, putative n=2 Tax=fabids RepID=B9RK44_RICCO	-	-	0.0	81%
FL-Next	sp=Threonine synthase, chloroplastic; Solanum tuberosum (Potato).	-	-	0.0	84%
Sma3	Threonine synthase	-	-	2.073e-15	-

Source	ECs	Term	Type	e value	Identity
Sma3	Threonine synthase.	EC:4.2.3.1	-	4.428e-23	-

Source	KEGGs	Term	e value	Identity
Sma3	Glycine, serine and threonine metabolism	00260	4.428e-23	%
Sma3	Vitamin B6 metabolism	00750	4.428e-23	%
Sma3	Metabolic pathways	01100	4.428e-23	%

Source	Gene names
Sma3	At1g72810; At4g29840; CHLREDRAFT_127384; F27B13.80; F3N23.1; GSVIVT00016507001; GSVIVT00032716001; MICPUCDRAFT_45441; MICPUN_60673; OSTLU_37018; Os01g0693800; Os05g0549700; OsI_03360; OsI_20875; OsJ_03101; OsJ_19448; Ot04g01610; P0431H09.7; P0560C03.1; PH

Source	GOs	Term	Type	Identity
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	threonine synthase activity	GO:0004795	Molecular Function	-
Sma3	pyridoxal phosphate binding	GO:0030170	Molecular Function	-
Sma3	cellular amino acid metabolic process	GO:0006520	Biological Process	-
Sma3	threonine biosynthetic process	GO:0009088	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	IPR000634	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Pyridoxal phosphate-dependent enzyme, beta subunit	IPR001926	-	-
Sma3	Threonine synthase	IPR004450	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G29840.1	MTO2, TS Pyridoxal-5'-phosphate-dependent enzyme family protein chr4:14599434-14601014 REVERSE LENGTH=526	83%
RefSeq	Arabidopsis thaliana	NP_194713.1	threonine synthase 1 [Arabidopsis thaliana]	83%
RefSeq	Populus trichocarpa	XP_002329027.1	predicted protein [Populus trichocarpa]	84%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q9MT28

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 268 - 519

Fln protein: CIASRLHS*CSRLPMVRWCSVSILISMGCMRLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIVPGGNLGNIYAFYKGFQMCKELGLVNDIPRLVCAQAANANPLYLYYKSGWNEFKPVTANSTFASAIQIGDPVSIDRAVYALKNSDGIVEEATEEELMDAAALADLKGMFICPHTGVALAALTKLRSKGIIGLNDRTVVVSTAHGLKFTQSKIDYHSKAIPDMKCEYANPPFPVKAEFSSVMDVLKKKLALL*

Protein Length: 269

Fln nts: AATGTATCGCATCTCGATTGCACAGTTAGTGCAGCCGATTGCCAATGGTGCGTTGGTGCTCAGTCTCGATACTGATTTCGATGGGGTGTATGAGGCTGATCCGGGAGGTGACCGCAGAGTTGCCGATCTATCTGGCGAATTCGCTCAATAGTCTGCGGTTGGAGGGGCAGAAGACTGCAGCGATCGAGATCCTGCAGCAGTTTGATTGGGAGGTGCCAGATTGGGTCATTGTGCCTGGCGGGAACTTGGGAAATATCTATGCCTTTTATAAGGGGTTCCAGATGTGCAAGGAGTTGGGCCTGGTGAATGATATTCCTCGCCTTGTCTGTGCACAGGCTGCCAACGCCAATCCTCTGTATTTGTACTATAAGTCCGGTTGGAATGAATTTAAACCTGTGACTGCCAATTCCACCTTTGCCTCGGCCATACAGATTGGTGATCCCGTTTCAATCGATCGAGCTGTATATGCACTGAAGAATTCAGATGGAATAGTCGAGGAGGCTACCGAGGAGGAATTGATGGATGCTGCAGCTCTGGCAGACCTGAAAGGTATGTTTATATGCCCTCATACTGGAGTGGCTCTTGCAGCATTGACAAAGCTCAGAAGTAAGGGCATTATTGGGCTTAATGACAGGACCGTTGTTGTCAGTACTGCTCATGGGCTGAAGTTTACACAATCCAAGATTGACTATCACTCAAAAGCGATTCCTGACATGAAATGCGAATATGCAAACCCTCCTTTTCCAGTAAAAGCAGAATTTTCTTCTGTTATGGATGTTTTGAAGAAGAAACTAGCTCTTCTTTGA___GTCGTGGATACTGGCTATGGGGCCAGGCGTTGGTCTCAACTATTTTCAGAAGGCCACAGCCTCTTATGCTGTCCCAAATGGTTCTGTGTGTTGGTTTATTTGACATCGCTTCTCTGTGGCTTTCCCGTATTCATTGAGCTATCAGCACATTGAGGGAAAGTTTTTTCCTTTCCGATATACCAGAGCCACTGTTTTCAGTCATTGTTAACGTTATGGTGGGTGCAGTTCACTTCTCTCGGCTAATCATGGTATAGAGTTTAGCAAGTTGATCTTTCATCATATATCCTGTTGTCTGGTTTATCATGGAAATTTTGTTGGGAGCCAAATGGTTTATCATGGAAAGTTTGGCTGGAAATTTTGTTTGAATTTTT

Fln Alignment:

AllPine_a_c26934___GCMRLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIVPGGNLGNIYAFYKGFQMCKELGLVNDIPRLVCAQAANANPLYLYYKSGWNEFKPVTANSTFASAIQIGDPVSIDRAVYALKNSDGIVXXXXXXXXXXXXXXXXXKGMFICPHTGVALAALTKLRSKGIIGLNDRTVVVSTAHGLKFTQSKIDYHSKAIPDMKCEYANPPFPVKAEFSSVMD
Q9MT28________________GCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFALQQCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVSTAHGLKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVMD

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
334	20	80			0.999996
351	80			20	0.999996
352		80		20	0.999996
366	80			20	0.999996
383		80		20	0.999996
386			22	77	0.999999
401	75		25		1.0
404		28		71	1.0
425		22		77	0.999999
428	25	75			1.0
434		75	25		1.0
455			25	75	1.0
473	85			14	0.993533
477	14		85		0.993533
480		71		28	1.0
524	85		14		0.993533
553		85		14	0.993533
580		66		33	0.999991
625		50		50	1.0
838		25	75		0.996473
840			75	25	0.996473
842		75	25		0.996473
843	75			25	0.996473
845	25		75		0.996473
847		25	75		0.996473
854		75	25		0.996473
859	75			25	0.996473
861	25			75	0.996473
877	25	75			0.996473
893		66		33	0.997452
1022	50			50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain