SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene99494

Length: 1405 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene99494 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	phytochelatin synthase 1 [Nicotiana tabacum]	-	-	0.0	58%
FL-Next	sp=Glutathione gamma-glutamylcysteinyltransferase 1; Lotus japonicus (Lotus corniculatus var. japonicus).	-	-	0.0	56%
Sma3	Phytochelatin synthase	-	-	1.325e-40	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glutathione gamma-glutamylcysteinyltransferase.	EC:2.3.2.15	-	3.721e-27	-

Source	Gene names
Sma3	ARA8; At1g03980; At5g44070; AyPCS1; BjPCS1; CAD1; CHLREDRAFT_98303; F21M11.9; FePCS; GSVIVT00028555001; MRH10.11; MRH10_18; OJ1123_C08.7; OSJNBa0075G19.14-1; Os05g0415200; Os06g0102300; OsI_19969; OsI_21267; OsJ_18552; OsJ_19803; PCS; PCS1; PCS2; PCS3; PC

Source	GOs	Term	Type	Identity
Sma3	plastid	GO:0009536	Cellular Component	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	copper ion binding	GO:0005507	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3		GO:0008415	Molecular Function	-
Sma3	glutathione gamma-glutamylcysteinyltransferase activity	GO:0016756	Molecular Function	-
Sma3	cadmium ion binding	GO:0046870	Molecular Function	-
Sma3	metal ion binding	GO:0046872	Molecular Function	-
Sma3	response to metal ion	GO:0010038	Biological Process	-
Sma3	response to arsenic-containing substance	GO:0046685	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	phytochelatin biosynthetic process	GO:0046938	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Phytochelatin synthase	IPR007719	-	0.0	-
Sma3	Phytochelatin synthase, C-terminal	IPR015407	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G44070.1	CAD1, ARA8, ATPCS1, PCS1 phytochelatin synthase 1 (PCS1) chr5:17734876-17737672 FORWARD LENGTH=485	58%
RefSeq	Arabidopsis thaliana	NP_199220.1	glutathione gamma-glutamylcysteinyltransferase 1 [Arabidopsis thaliana]	58%
RefSeq	Populus trichocarpa	XP_002320626.1	predicted protein [Populus trichocarpa]	59%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: Q2TSC7

Fln msg: Distance to subject end: 50 aas, your sequence is shorter than subject: 439 - 501

Fln protein: MAVAGLYRRHLTCPPAVDFSSSEGKELFCEALQNGTMEGFFKLISYFQTQSEPAYCGLATLAVVLNALAIDPGRKWKGPWRWFDESMLDCCEPLEKVKVDGITFGKVACLANCSGAKVEAFRSNQSSIDEFRRFVEKCATSEDCHVITSYHRAPLKQTGSGHFSPIGGYHAGKDMALILDVARFKYPPHWVPLPLLWEAMNTIDETTQKYRGFMLILKHQQGPSLLYTLSCKHESWKIISKYLMEQVPQLLKAEGFDSIEKILSAVFESLPANLTDFIKWIVEVRRLESSEKTVDKEEAERIAIKGELLQQIKETEIYKVVDKWSSSATSCCTLSWSTEDNSSEIIENVCCQREATQCELIGVSKECFCNKACMQSASTSDNFPVAIVSGTVPAFGSEKGIDMLVPTTKNVCRQGAATQCGRIGVANECVCKKACMKSM

Protein Length: 440

Fln nts: TACGTTCTTAGTCTTCTAATTTGGCCACGCGGCTGTCTGAACCTCTGGTTTGGTAAACTGAAATTAAATAATAGTGGAAGTGAGTGG_-_ATGGCGGTTGCAGGTCTCTACAGGCGCCACCTCACTTGCCCTCCGGCTGTGGATTTCTCCTCCTCCGAAGGCAAGGAACTCTTTTGCGAGGCTCTACAAAATGGGACCATGGAAGGATTCTTTAAACTCATTTCATACTTCCAAACACAATCTGAACCTGCCTACTGTGGATTGGCTACTCTTGCAGTTGTTCTTAATGCTCTTGCAATTGATCCTGGAAGAAAATGGAAGGGGCCTTGGCGCTGGTTTGATGAATCAATGCTGGACTGTTGTGAACCTTTGGAAAAGGTGAAAGTTGATGGCATCACATTTGGGAAAGTGGCATGCCTAGCTAATTGTTCTGGAGCTAAGGTTGAAGCTTTCCGCTCTAATCAAAGTTCTATTGATGAATTCCGGAGATTTGTAGAAAAGTGTGCTACTTCTGAAGATTGCCATGTGATTACATCATATCATAGAGCTCCACTAAAGCAGACAGGATCTGGCCATTTTTCACCGATTGGTGGATACCATGCTGGAAAAGACATGGCACTTATTTTAGATGTCGCTCGATTCAAGTATCCTCCCCATTGGGTTCCACTTCCACTTCTTTGGGAGGCAATGAATACCATAGATGAAACAACTCAAAAATACAGGGGGTTTATGCTAATCTTGAAGCACCAGCAGGGTCCATCCTTACTTTATACTCTGTCCTGCAAACATGAAAGTTGGAAAATCATTTCAAAGTATCTTATGGAGCAGGTTCCGCAGCTTCTGAAAGCCGAAGGTTTTGACAGCATAGAAAAAATCCTTTCTGCAGTTTTTGAATCATTACCTGCAAATCTTACAGATTTCATCAAGTGGATTGTGGAAGTTAGAAGGTTGGAAAGCTCTGAGAAAACTGTAGATAAGGAAGAAGCTGAGCGAATTGCTATCAAGGGAGAATTATTGCAACAAATAAAGGAAACAGAAATCTACAAGGTTGTGGATAAGTGGTCTTCATCGGCAACCTCATGCTGCACTTTATCATGGTCAACTGAAGATAATTCGTCAGAGATAATTGAAAATGTTTGCTGCCAAAGGGAAGCCACCCAATGTGAACTGATTGGTGTATCGAAAGAGTGTTTTTGTAATAAGGCTTGTATGCAAAGCGCAAGCACAAGTGACAATTTCCCAGTGGCAATTGTTTCTGGAACTGTACCTGCATTTGGTAGTGAGAAAGGCATTGATATGCTAGTTCCCACTACTAAAAATGTTTGTCGCCAAGGTGCAGCCACCCAATGTGGAAGGATTGGTGTAGCCAATGAGTGTGTCTGTAAGAAGGCTTGTATGAAAAGCATGA

Fln Alignment:

AllPine_a_c22284___MAVAGLYRRHLTCPPAVDFSSSEGKELFCEALQNGTMEGFFKLISYFQTQSEPAYCGXXXXXXXXXXXAIDPGRKWKGPWRWFDESMLDCCEPLEKVKVDGITFGKVACLANCSGAKVEAFRSNQSSIDEFRRFVEKCATSEDCHVITSYHRAPLKQTGSGHFSPIGGYHAGKDMALILDVARFKYPPHWVPLPLLWEAMNTIDETTQKYRGFMLILKHQQGPSLLYTLSCKHESWKIISKYLMEQVPQLLKAEGFDSIEKILSAVFESLPANLTDFIKWIVEVRRLESSEKTVDKEEAERIAIKGELLQQIKETEIYKVVDKWSSSATSCCTLSWSTEDNSSEIIENVCCQREATQCELIGVSKECFCNKACMQSASTSDNFPVAIVSGTVPAFGSEKGIDMLVP------------TTKNVCRQGAAT
Q2TSC7_______________MAMAGLYRRLLPSPPAVDFASSQGKQLFLEAVQNGTMESFYRLVSYFQTQSEPAFCGLASLSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPLDKIKARGISFGKLVCLAHCAGAKVEAFHASHSSIDHFRKYVMKCSTSDDCHVISSYHREALKQTGTGHFSPIGGYHAGKDMALILDVARFKYPPHWIPLTHLWEGMNYVDESTGKTRGFMLISRPHREPGMLYTLSCKHESWNSIAKFLIDDIPFLLTSEDVKDICKVLSVIVTSLPSNFEEFIKWVAEIRRGEDGSPSLSVEEKARLSVKEEILKQVQRTGLFKHVASFLSHSCSGHTPTSGDRDTFPVIAASVCCQGAEILGGKISSSAEYCCRETCMKCWKAEDDKPIRMVCGTVVNGNTEQGVDVLIPSSCGKLSCTCSSTTKSIRKHPAST

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
561			75	25	1.0
629		87		12	0.992554
649			87	12	0.992554
652			12	87	0.992554
653			87	12	0.992554
657	12	87			0.992554
658		87	12		0.992554
659		12		87	0.992554
660		12	87		0.992554
661	12	87			0.992554
662	12			87	0.992554
664		12	87		0.992554
665	12		87		0.992554
667			75	12	0.975346
669	14			85	0.993533
670		14		85	0.993533
671		85	14		0.993533
673	85	14			0.993533
704		50		50	1.0
749	66		33		0.997458
832		33		66	0.997453
1031	66		33		0.997452
1070		50		50	1.0
1255	25	75			0.996479
1256			25	75	0.996475
1259		75	25		0.996475
1297			33	66	0.997464

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain