SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene96893

Length: 1435 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene96893

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[I] COG0657 Esterase/lipase	-	-	2.0e-17	33%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	73%
Sma3	CXE carboxylesterase	-	-	2.177e-24	-

Source	ECs	Term	Type	e value	Identity
Sma3	Carboxylesterase.	EC:3.1.1.1	-	2.015e-22	-

Source	KEGGs	Term	e value	Identity
Sma3	Tropane, piperidine and pyridine alkaloid biosynthesis	00960	2.015e-22	%
Sma3	Drug metabolism - other enzymes	00983	2.015e-22	%
Sma3	Metabolic pathways	01100	2.015e-22	%
Sma3	Biosynthesis of secondary metabolites	01110	2.015e-22	%

Source	Gene names
Sma3	At3g48700; At3g48700/T8P19_210; B1342C04.22; BIG8.1; CXE1; CXE10; CXE11; CXE3; CXE5; CXE6; CXE8; GSVIVT00014792001; GSVIVT00019941001; GSVIVT00019942001; GSVIVT00032640001; GSVIVT00032645001; GSVIVT00032646001; GSVIVT00032648001; GSVIVT00036557001; GSVIVT

Source	GOs	Term	Type	Identity
Sma3	carboxylesterase activity	GO:0004091	Molecular Function	-
Sma3	hydrolase activity	GO:0016787	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Carboxylesterase, type B	IPR002018	-	-
Sma3	Lipase, GDXG, active site	IPR002168	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Alpha/beta hydrolase fold-3	IPR013094	-	-
Sma3	Peptidase S26A, signal peptidase I, serine active site	IPR019756	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9P0Y8

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 21 aas, atg_distance in limit (1-15): atg_distance = 3, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 320 - 357

Fln protein: MESPSPLSIIVEAPGFVRVYEDGTVERVVDLGTVPPSPELESGDLHGVASKDVVINPETGVFVRLYLPRLEVTDGKEKVSVLVYVHGGGFCIESAASPFYHNYLNKVCTEAKVICVSVEYRRAPEHRLPAAYDDCFGVLEWLALQARAAECVPIDPWLVSYADFRNVFLAGDSAGANIVHQMGIRASARNWDGVCLQGAILVHPAFGSEERIGRELGPEAEVEDFNKLTDVIWSISLPAGADKDHPFSNPVGPRSPDLSSLVYGRILVFVAEKDLLRDRGVLYYEALKKAGKDADLVVTEGEDHVFIFLIRNLRMHPPC*

Protein Length: 321

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTTGGCGTCATCTAAAGTGCAACATCT_-_ATGGAGTCGCCGTCACCCTTGTCTATCATTGTTGAAGCCCCTGGTTTCGTGAGAGTTTATGAAGACGGAACGGTTGAACGCGTGGTCGATCTGGGTACGGTTCCGCCTTCTCCAGAGCTCGAATCTGGTGATTTGCATGGAGTGGCATCCAAAGACGTGGTCATCAATCCTGAAACAGGCGTCTTCGTTCGTCTCTACCTCCCCCGGCTTGAGGTTACCGATGGAAAAGAAAAAGTGTCCGTGCTTGTTTATGTTCACGGAGGGGGTTTCTGCATTGAATCCGCTGCTTCGCCATTTTATCACAACTATCTTAATAAAGTGTGTACTGAGGCCAAGGTGATTTGTGTATCGGTGGAGTACCGGAGAGCCCCTGAGCACCGCCTCCCTGCGGCTTATGACGATTGTTTCGGTGTGCTCGAATGGCTCGCCCTCCAAGCAAGGGCAGCCGAGTGTGTGCCCATCGATCCGTGGCTCGTTTCCTACGCAGATTTCAGAAACGTGTTCTTGGCAGGTGACAGTGCTGGTGCCAATATCGTGCACCAGATGGGCATTCGTGCAAGTGCAAGGAATTGGGATGGGGTGTGTTTACAAGGGGCCATTCTGGTTCACCCCGCCTTTGGCAGCGAAGAACGAATTGGCCGCGAACTGGGGCCAGAGGCGGAGGTGGAGGATTTTAATAAGTTGACGGACGTAATATGGAGCATCTCTCTCCCCGCAGGGGCGGACAAGGACCATCCTTTCTCCAACCCAGTTGGACCCCGTTCCCCTGATTTGTCCAGTCTGGTGTACGGACGAATTCTTGTATTTGTTGCAGAGAAGGATCTTCTGAGAGATCGGGGTGTTTTATATTACGAAGCACTAAAGAAGGCGGGCAAAGATGCCGATTTGGTTGTGACGGAAGGCGAAGACCATGTATTCATCTTTTTAATCCGAAATCTGAGAATGCACCCCCCATGTTGAAACGCATTTCGGACTTTATGCATTCCTCCTCCTACGATCTGTGCAAGAAACCCACGCATAATTCCTCCTCCTCCGATCAGTGCAAGAACCCCACGGAAAATCCTTTCATATGATAACATGGAGTGAATATTGAAGAAATATGGGTGTCATTATTATTATTCAGATGTTGAAATTAATGTCTCCGTTTTTGTTGACTTTATTGCACTTATTTTGAACTGACGACTCTGTTAGACGTTATAGCTGTTTCTCATCTCCTACATGATTAGGGTAGTAGTTTTTGTCATCTCCTCCAGCGAATGGTCTCGTTTGAGTATACACTGGTTGGTTGATACATAGTCTCTGTGTGGTCTCTGTTGAGTGTTTGTCATGGAATGTGGTCCGTTAGTCCATAGCAAAGAGCATACAATTTCAGGTTCATCCTC

Fln Alignment:

AllPine_a_c16353___SATSMES-PSPLSIIVEAPGFVRVYEDGTVERVVDLGTVPPSPELESGDLHGVASKDVVINPETGVFVRLYLPRLEVTDGKEKVSVLVYVHGGGFCIESAASPFYHNYLNKVCTEAKVICVSVEYRRAPEHRLPAAYDDCFGVLEWLALQARAAECVPIDPWLVSYADFRNVFLAGDSAGANIVHQMGIRASARNWDGVCLQGAILVHPAFGSEERIGRELGPEAEVEDFNKLTDVIWSISLPAGADKDHPFSNPVGPRSPDLSSLVYGRILVFVAEKDLLRDRGVLYYEALKKAGKDADLVVTEGEDHVF
A9P0Y8_______________NAIPMESSPSSLSIVAEAPGMLRIYEDGTVDRLIDSSTVPPSSQLGDESREGVASEDVVIDPQTGVFVRIFLPRLE---GKQKVPVLVYFHGGAFCIGSAVSPIYHNYVNEVASEAKVICLSVEYRKAPEHRLPAAYYDGFGVLEWLNRQAEAEEGAPVDPWLASHADFSNVFLAGDSAGGNIVHQVGILASGRNWDGLCLQGAILVHPAFGGKELIGWEVEPEGESQNFSKFSDAIWGISLPPGADKDHPFSNPVGPRSPALSTLEYGRILVFVAEKDLLRDRAVLYYEALKKAGKDADLVMAEGEDHVF

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
159	33		66		0.997452
215		33		66	0.997452
238		66		33	0.997452
269	33		66		0.997452
278		33	66		0.997476
331		33		66	0.997452
613			25	75	0.996473
652	75		25		0.996473
708		50		50	1.0
731	50		50		1.0
770		50		50	1.0
803		66		33	0.997452
828		83		16	0.994512
829		83		16	0.994512
1182	66	33			0.997452
1183			33	66	0.997452
1187	66			33	0.997452
1190		33		66	0.997452
1191		33	66		0.997452
1196	66	33			0.997452
1197	66			33	0.997452
1198	66		33		0.997452
1200		33		66	0.997452
1202	66	33			0.997452
1205	33		66		0.997452
1218		33		66	0.997452
1246	66			33	1.0
1247			66	33	0.997459
1248	66			33	0.997459
1315		66		33	0.997458
1328		33		66	0.997452
1341	33		66		0.997452
1342		33		66	0.997452
1410	33	66			0.997476
1413		33		66	0.997455
1414	66		33		0.997454

SSRs (tot: 2)

Start position	End position	Sequence	Length
1076	1087	TCC TCC TCC TCC	12
1162	1173	ATT ATT ATT ATT	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain