SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene37311

Length: 1156 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene37311

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam00759, Glyco_hydro_9, Glycosyl hydrolase family 9	-	-	0.0	40%
FL-Next	tr=Endo-beta-1,4-glucanase; Pinus radiata (Monterey pine) (Pinus insignis).	-	-	0.0	48%
Sma3	Endo-1,4-beta-glucanase	-	-	1.474e-36	-

Source	ECs	Term	Type	e value	Identity
Sma3	Cellulase.	EC:3.2.1.4	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Starch and sucrose metabolism	00500		0.0	%

Source	Gene names
Sma3	6J23.18; Al-cel1; At1g02800; At1g19940; At1g22880; At1g23210; At1g48930; At1g64390; At1g70710; At1g71380; At1g75680; At2g32990; At2g44540; At2g44550; At2g44560; At2g44570; At3g43860; At4g02290; At4g09740; At4g11050; At4g23560; At4g38990; At4g39000; At4g39

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	NADH dehydrogenase (ubiquinone) activity	GO:0008137	Molecular Function	-
Sma3	cellulase activity	GO:0008810	Molecular Function	-
Sma3	carbohydrate binding	GO:0030246	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	cellular cell wall organization	GO:0007047	Biological Process	-
Sma3	pattern specification process	GO:0007389	Biological Process	-
Sma3	response to nematode	GO:0009624	Biological Process	-
Sma3	fruit ripening	GO:0009835	Biological Process	-
Sma3	cellulose catabolic process	GO:0030245	Biological Process	-
Sma3	cell wall modification involved in multidimensional cell growth	GO:0042547	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 9	IPR001701	-	-
Sma3	Six-hairpin glycosidase	IPR012341	-	-
Sma3	NADH:ubiquinone oxidoreductase, B12 subunit	IPR012576	-	-
Sma3	Glycoside hydrolase, family 9, active site	IPR018221	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G43860.1	AtGH9A4, GH9A4 glycosyl hydrolase 9A4 chr3:15707183-15709438 FORWARD LENGTH=486	54%
RefSeq	Arabidopsis thaliana	NP_189972.2	endoglucanase 16 [Arabidopsis thaliana]	54%
RefSeq	Populus trichocarpa	XP_002308519.1	predicted protein [Populus trichocarpa]	57%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: O64402

Fln msg: your sequence is shorter than subject: 284 - 515

Fln protein: LFDFADQYRGTYDGECPFYCSFSGYDDELAWAASWLYRATRSPKYSQYILQQNFSTSNVSEFSWDLKSAGVQVLLSNLYFSGESIFEIYKNQAEMFFCSVLPFSPHFQHSMTPGGLLYVRDGANTQYATSTSFLLLAYSDLLSSHNQSLHCDYQIYRADDLIAFAKQQIHYILGWNPLGMSFMVGFGDRYPQQAHHRGSSIVSIYETTAKVSCTEGFMVWFRRQAPNPNELTGAVVGGPDRYDNFEDYRADSCKLEPTTYINAPLVGVLARLHTISMGSPVTT*

Protein Length: 285

Fln nts: CTGTTTGATTTTGCTGACCAGTACCGTGGAACATATGATGGAGAATGCCCTTTCTACTGTTCTTTCTCAGGCTATGACGACGAGCTTGCATGGGCAGCGTCATGGCTGTATAGAGCAACCAGATCACCAAAGTATTCCCAATACATACTCCAGCAGAATTTCTCTACTTCCAATGTATCTGAGTTCAGCTGGGATCTCAAATCTGCGGGAGTTCAAGTCCTGCTCTCTAATCTATATTTCTCTGGAGAATCCATCTTTGAAATTTACAAAAACCAAGCAGAAATGTTCTTCTGCTCAGTTCTTCCATTTAGTCCTCACTTCCAGCATTCCATGACACCAGGAGGATTGCTGTATGTGAGAGATGGTGCAAATACACAATATGCTACAAGCACATCATTCCTCCTATTGGCTTACAGTGACCTATTGTCAAGCCACAACCAATCCCTGCATTGTGATTATCAAATCTACCGGGCGGACGATCTTATTGCATTTGCCAAACAACAAATCCATTACATATTGGGCTGGAATCCACTGGGTATGTCTTTCATGGTGGGATTTGGTGACAGATATCCACAGCAAGCTCATCACAGAGGATCATCTATAGTTTCGATATACGAGACAACGGCAAAGGTATCATGCACAGAAGGTTTCATGGTGTGGTTCCGCAGACAAGCACCGAACCCCAATGAATTAACAGGTGCAGTTGTAGGAGGGCCTGATCGCTATGATAATTTTGAAGATTACAGGGCCGATTCGTGCAAGCTTGAGCCCACAACTTATATCAATGCTCCTCTCGTGGGGGTCCTTGCCAGGCTCCACACCATCAGCATGGGGTCTCCAGTTACAACTTAG___TAACTTCACCTAGATTTTTTGCTTGGTATTTGTTCTGGGCTGTTTTGCTCATTTCACCTTTGGATCTTACAGCCAGATTAAGTTTGATAGGGCTTGTAAATCAATTTTTCCTCAATTTTATGAAAATAGAATTAAAGCTACGAATTTAGATTTTAGTGTGACAATGAGTGTTAAGTGGACTATTTAAAAATCATGAGAAATTTGTCATTTACGACTATTTCTCATGGAAATGGTTATAGATGAATGACTTGCTTAAGACAGTTGAATCCCAAAAAAAATAATATCTAATCATTAGTGTTATCAT

Fln Alignment:

Contig37311___LFDFADQYRGTYDGE-----CPFYCSFSGYDDELAWAASWLYRATRSPKYSQYILQ--QNFS-TSNVSEFSWDLKSAGVQVLLSN-LYFSGESIFEIYKNQAEMFFCSVLPFSPHFQHSMTPGGLLYVRDGANTQYATSTSFLLLAYSDLLSSHNQSLHCDYQIYRADDLIAFAKQQIHYILGWNPLGMSFMVGFGDRYPQQAHHRGSSIVSIYETTAKVSCTEGFMVWFRRQAPNPNELTGAVVGGPDRYDNFEDYRADSCKLEPTTYINAPLVGVLARL
O64402_______________VFDFADKHRGAYSDSLQSEVCPFYCSYSGYNDELLWGAAWLQRASRNVSYISYIQSHGQNLGGEDNVNIFSWDDKHAGARVLLAKEVLLRNSKSLEEYRGHADNFVCSLLPGTPNSQAQYTPGGLLYKMSDCNLQYVTSTTFLLFTYPKYLRVSKQVVRCGNMVVTPTRIRTLAKRQVDYILGDNPLRMSYMVGYGAKFPERIHHRGSSLPSIYQHPQIIPCNDGFQSLY-SNAPNPNRLVGAVVGGPDNNDHFSDERNDYAHSEPTTYINAPLVGSLAYL

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
411	40		60		1.0
436	80		20		0.995493
452	40			60	1.0
607	16		83		0.999991
844			87	12	0.992554
845			14	85	0.993533
846			14	85	0.993533
847	85		14		0.993533
926		80		20	0.995493
947		20	80		0.995493
949		20	60		0.99255
962	25			75	0.996473
968		75		25	0.996473
995		50		50	1.0
1001	33			66	0.997452
1005	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain