SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene37230

Length: 1984 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene37230
ATGGGGATGGCCGTTACTCGGAGGGACACTGCCCTTTCTTAAACCCCATCCTTCCACTTCCACAGGCCCATTTCTTCACCAACGCGTGCAACGGTTCGGAAGAGTTTTCAAGTCCCATCTATTTGGGGAGCCCGCAATTGTATCCTGCGATCCCGATCCCAATGCTTACGTGCTGCAGAACGAGGACCGGTTGTTCCAGTGCAGCTACCCCACGGCCATTCATGGAATATTGGGCGAAATGTCTATGCTTGTGGTTGTCGGCGATGCTCACAAACGATTGCGAACCCTGGCACTCAGTCTGGTTAATGCTGCCAAAACCAATCCCAACTTCCTCAGTGATATAGAAGCGAATGCACTTCTCATCACTGGTAAATGGAAGCAAAAACAGACCGTACTCTTCTGTGAAGAGGCCAGAAAGTTCACATTCACTGTAATAGTGAAACAAATTCTGAGTTTGAAGCCAGATAGTCCGGAAACATCCACCATCTTGAAGGACTTTCTTACTTTCATGAAAGGACTGGCGTCACTGCCGCTATATATTCCCGGAACATCTTACGCAAAAGCTGTCAAGTCGAAATCTCAAATTCTTTCCACCCTTAAAACGCTGGTGGATAGGAGGAGGAATGAAGGCATTCAGAGAGGAGATTTTCTTGATATGCTTCTGACAAGCACGAGTCTAGGCGATGATGAGATCCTTAGCCTGGTTTTAGATCTTCTGCTTGGAGGCTATGAAACAACAGCAATTTTAATAGCTATGGTAGTCAAATTTCTCACAGACTGCGCTTCTGCTTTCCAGCAGTTAAAGAGTGAGCACGAGAAAATCAGAAGGGGAAAACAGAAGGGTGAAGCAGGTTTAAATTGGGATGATTACAGGCAAATGTCTTTTACACAAAATGTAATCAACGAGGCACTGCGGTGTGGGAACGTGGTTAAATTTGTTCACCGGAAAGCTCTGAAAGACGTAAAGTTTAAAGGGTACGACATTCCGGCAGGATGGAAGATTCTCCCAATGTTCAGTGCTGTTCATCTAGACTCCTCTGCCTTTAAAGACCCTCAAGAGTTCAATCCCTGGAGATGGCAGACAGCGATAGCAGGAAAACAATTTACACCTTTTGGTGGAGGACCGAGGCTTTGTCCTGGATCAGAATTGGCTAAAGTCGAGACTGCTTTTTTCATTCACCATCTGGTTCTGAACTTCAGATGGAGAGCAGCTGAGCTGGATCACTCTGTTGCATATCCCTATGTGGAATTCGAGAAGGGGTTGCCAATCCACGTTGAGCGTGTGTGTATGGCGGATTAAATTTAGGATTCCATTTTGGTCTTTATGCATAAATAATTGTTTATGACAGCAGAAAGATGAAGGACAGCAGTCACAGCACGCTCACTCGCTCATGTGTCCTGTACTTGGGATTTCTAGCGGATCAGGACTCTGGCAACTTACATGAAGAAGTGATGATGATCCAGTGAAGGTCCCATATCGCTAATTTAATGACAGACATCATTGCATTGGTGGTCAATGGAGTCGCGATACGGATATATCAAATACAGGAAAGAATTTCTTGGGATATGTGGATTCGACAGTGAGTTCGTCGAACGTGTGTATAAAAAATTGAGCTGTCAGCTTTTTGCTTCTCCCGGATATCGCCTTTGACTGTATTTTTTAATGCAGTACAATTAGTCAAATGTACTTCAGGATTTTTCAGTATAAATACAGATTTCTGAACATTTTCTGATCCTCCATAACAGTCAGTGCCAGTAGCAATTTGTCAGGGATATTTAAATAACGTTTTATTCCACGGCATGACACTCATTACCAGTAGCAAATTATTGTGCCAGTAGCAATTTGTGACGAACATTTAAATAACATTTTATTCCACTGCATGACACTTATTACCAGTAGCAAATGCAGTTGTTTATAATGTTATTTTTTGGAAGTTTTTTTGGGGAATTTTTCCGGTCAAAATCTGGCAGCAATTTATTGGAA

Ace file of the UniGene sp_v3.0_unigene37230 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	cytochrome P450 [Solanum lycopersicum]	-	-	0.0	57%
FL-Next	tr=Cytochrome P450 90A20; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	43%
Sma3	Steroid 22-alpha-hydroxylase	-	-	1.447e-11	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:1.14.-.-	-	6.872e-06	-
Sma3	Taxane 13-alpha-hydroxylase.	EC:1.14.13.77	-	9.079e-06	-

Source	KEGGs	Term	e value	Identity
Sma3	Diterpenoid biosynthesis	00904	9.079e-06	%
Sma3	Biosynthesis of terpenoids and steroids	01062	9.079e-06	%
Sma3	Metabolic pathways	01100	9.079e-06	%
Sma3	Biosynthesis of secondary metabolites	01110	9.079e-06	%

Source	Gene names
Sma3	AT5G05690; At3g50660; At5g05690; CPD; CYP724B1; CYP724B2; CYP724B7; CYP90; CYP90A; CYP90A1; CYP90A15; CYP90A2; CYP90A6v1; CYP90B; CYP90B1; CYP90B3; CYP90B7v1; D11; DWF4; GSVIVT00020626001; GSVIVT00024952001; GSVIVT00029049001; LOC_Os03g12660; LOC_Os04g394

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	steroid 22-alpha hydroxylase activity	GO:0010012	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	skotomorphogenesis	GO:0009647	Biological Process	-
Sma3	response to brassinosteroid stimulus	GO:0009741	Biological Process	-
Sma3	unidimensional cell growth	GO:0009826	Biological Process	-
Sma3	positive regulation of flower development	GO:0009911	Biological Process	-
Sma3	response to UV-B	GO:0010224	Biological Process	-
Sma3	brassinosteroid homeostasis	GO:0010268	Biological Process	-
Sma3	leaf shaping	GO:0010358	Biological Process	-
Sma3	brassinosteroid biosynthetic process	GO:0016132	Biological Process	-
Sma3	leaf development	GO:0048366	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Bacterioferritin	IPR002024	-	-
Sma3	Thiolase	IPR002155	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, E-class, group IV	IPR002403	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	Peptidase S26A, signal peptidase I, conserved site	IPR019758	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G50660.1	DWF4, CYP90B1, CLM, SNP2, SAV1, PSC1 Cytochrome P450 superfamily protein chr3:18814262-18817168 REVERSE LENGTH=513	45%
RefSeq	Arabidopsis thaliana	NP_190635.1	cytochrome P450 90B1 [Arabidopsis thaliana]	45%
RefSeq	Populus trichocarpa	XP_002321213.1	cytochrome P450 [Populus trichocarpa]	61%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NWM7

Fln msg: your sequence is shorter than subject: 433 - 487

Fln protein: WGWPLLGGTLPFLKPHPSTSTGPFLHQRVQRFGRVFKSHLFGEPAIVSCDPDPNAYVLQNEDRLFQCSYPTAIHGILGEMSMLVVVGDAHKRLRTLALSLVNAAKTNPNFLSDIEANALLITGKWKQKQTVLFCEEARKFTFTVIVKQILSLKPDSPETSTILKDFLTFMKGLASLPLYIPGTSYAKAVKSKSQILSTLKTLVDRRRNEGIQRGDFLDMLLTSTSLGDDEILSLVLDLLLGGYETTAILIAMVVKFLTDCASAFQQLKSEHEKIRRGKQKGEAGLNWDDYRQMSFTQNVINEALRCGNVVKFVHRKALKDVKFKGYDIPAGWKILPMFSAVHLDSSAFKDPQEFNPWRWQTAIAGKQFTPFGGGPRLCPGSELAKVETAFFIHHLVLNFRWRAAELDHSVAYPYVEFEKGLPIHVERVCMAD*

Protein Length: 434

Fln nts: ATGGGGATGGCCGTTACTCGGAGGGACACTGCCCTTTCTTAAACCCCATCCTTCCACTTCCACAGGCCCATTTCTTCACCAACGCGTGCAACGGTTCGGAAGAGTTTTCAAGTCCCATCTATTTGGGGAGCCCGCAATTGTATCCTGCGATCCCGATCCCAATGCTTACGTGCTGCAGAACGAGGACCGGTTGTTCCAGTGCAGCTACCCCACGGCCATTCATGGAATATTGGGCGAAATGTCTATGCTTGTGGTTGTCGGCGATGCTCACAAACGATTGCGAACCCTGGCACTCAGTCTGGTTAATGCTGCCAAAACCAATCCCAACTTCCTCAGTGATATAGAAGCGAATGCACTTCTCATCACTGGTAAATGGAAGCAAAAACAGACCGTACTCTTCTGTGAAGAGGCCAGAAAGTTCACATTCACTGTAATAGTGAAACAAATTCTGAGTTTGAAGCCAGATAGTCCGGAAACATCCACCATCTTGAAGGACTTTCTTACTTTCATGAAAGGACTGGCGTCACTGCCGCTATATATTCCCGGAACATCTTACGCAAAAGCTGTCAAGTCGAAATCTCAAATTCTTTCCACCCTTAAAACGCTGGTGGATAGGAGGAGGAATGAAGGCATTCAGAGAGGAGATTTTCTTGATATGCTTCTGACAAGCACGAGTCTAGGCGATGATGAGATCCTTAGCCTGGTTTTAGATCTTCTGCTTGGAGGCTATGAAACAACAGCAATTTTAATAGCTATGGTAGTCAAATTTCTCACAGACTGCGCTTCTGCTTTCCAGCAGTTAAAGAGTGAGCACGAGAAAATCAGAAGGGGAAAACAGAAGGGTGAAGCAGGTTTAAATTGGGATGATTACAGGCAAATGTCTTTTACACAAAATGTAATCAACGAGGCACTGCGGTGTGGGAACGTGGTTAAATTTGTTCACCGGAAAGCTCTGAAAGACGTAAAGTTTAAAGGGTACGACATTCCGGCAGGATGGAAGATTCTCCCAATGTTCAGTGCTGTTCATCTAGACTCCTCTGCCTTTAAAGACCCTCAAGAGTTCAATCCCTGGAGATGGCAGACAGCGATAGCAGGAAAACAATTTACACCTTTTGGTGGAGGACCGAGGCTTTGTCCTGGATCAGAATTGGCTAAAGTCGAGACTGCTTTTTTCATTCACCATCTGGTTCTGAACTTCAGATGGAGAGCAGCTGAGCTGGATCACTCTGTTGCATATCCCTATGTGGAATTCGAGAAGGGGTTGCCAATCCACGTTGAGCGTGTGTGTATGGCGGATTAA___ATTTAGGATTCCATTTTGGTCTTTATGCATAAATAATTGTTTATGACAGCAGAAAGATGAAGGACAGCAGTCACAGCACGCTCACTCGCTCATGTGTCCTGTACTTGGGATTTCTAGCGGATCAGGACTCTGGCAACTTACATGAAGAAGTGATGATGATCCAGTGAAGGTCCCATATCGCTAATTTAATGACAGACATCATTGCATTGGTGGTCAATGGAGTCGCGATACGGATATATCAAATACAGGAAAGAATTTCTTGGGATATGTGGATTCGACAGTGAGTTCGTCGAACGTGTGTATAAAAAATTGAGCTGTCAGCTTTTTGCTTCTCCCGGATATCGCCTTTGACTGTATTTTTTAATGCAGTACAATTAGTCAAATGTACTTCAGGATTTTTCAGTATAAATACAGATTTCTGAACATTTTCTGATCCTCCATAACAGTCAGTGCCAGTAGCAATTTGTCAGGGATATTTAAATAACGTTTTATTCCACGGCATGACACTCATTACCAGTAGCAAATTATTGTGCCAGTAGCAATTTGTGACGAACATTTAAATAACATTTTATTCCACTGCATGACACTTATTACCAGTAGCAAATGCAGTTGTTTATAATGTTATTTTTTGGAAGTTTTTTTGGGGAATTTTTCCGGTCAAAATCTGGCAGCAATTTATTGGAA

Fln Alignment:

Contig37230___GWPLLGGTLPFLKPHPSTSTGPFLHQRVQRFGRVFKSHLFGEPAIVSCDPDPNAYVLQNEDRLFQCSYPTAIHGILGEMSMLVVVGDAHKRLRTLALSLVNAAKTNPNFLSDIEANALLITGKWKQKQTVLFCEEARKFTFTVIVKQILSLKPDSPETSTILKDFLTFMKGLASLPLYIPGTSYAKAVKSKSQILSTLKTLVDRRRNEGIQRGDFLDML---------LTSTSLGDDEIXXXXXXXXLGGYETTAILIAMVVKFLTDCASAFQQLKSEHEKIRRGKQKGEAGLNWDDYRQMSFTQNVINEALRCGNVVKFVHRKALKDVKFKGYDIPAGWKILPMFSAVHLDSSAFKDPQEFNPWRWQ----TAIAGKQFTPFGGGPRLCPGSELAKVETAFFIHHLVLNFRWRAAELDHSVAYPYVEFEKGLPIHVER
A9NWM7_______________GWPLLGETLQLIAAYKTSNPEPFVDTRRSRYGKVFKTHLFGKPTIFSTDPEVNKFILQNEGKLFATSYPSSITNLLGKHSLLLIQGNLHKRLHSLTLSFANSSVLKDHLMFDIEKLVCLTLDGWKDH--VLLQDEAKKITFELTVKQLLSFDP-GQWSENLRKEYLQLIDGFFSLPVPLPFTTYGKALKARAKVAEELRLVLKRRRDS--ESSDHYDMLAALLNEKSTTTSDGLSDDQIIDLLLSLLVAGYETTSTIMTLAVKFLTETPDALAQLKEEHEAIRKQK-PGAQRLEWSDYKSMAFTQCVISETLRIANIISGVFRRALCDVEIKGFNIPQDWLVFPCFRAVHLDQELYSDARKFNPWRWQGNHPSTSSGTVFTPFGGGPRLCPGYELARVEISVFLHHMVTLFSWEVVEEDKLIFFPTTRTVKRYPINLTR

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
1318			25	75	0.996473
1453		75		25	0.996473
1513		75		25	0.996473
1826	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain