SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene35248

Length: 574 nt

UniGene Fasta

>sp_v3.0_unigene35248
TTCGATCCGGACGGTGGTGCATTGAATGCCTAGCACGCAGTACGTATCGTATCTTGTTCCTGCCATGAACAACATTCAAACCGCGATCCAGAACGCCAATCTGCAGAACATCAAGGTCTCTACCCCCCATGCCTTCAATGTTATAGGCGTCAGTTACCCGCCTTCACATGGAGCATTCAGCGACGATGTGAAGGATACCATGAGCTATACTCAAATTTCTTTCAGATAACGGGGCTCCGTTCATGGCCAATGTGTATCCATATTTGAGTTACGCCGGCGACACCAACGGCATTCATCTGGACTACGCTCTCTTTCAGCCCACGGCTACGCCGGTGACAGACAAAGATCACAGCTACAGCAACCTGTTCGATGCCATGGTTGATACTCTTTTGTGGGCCATGGAAGCCTCGGGGTATCCCAACATCCCGATCGTCATTGCCGAAAGTGGATGGCCTTCTGCTGGCGCGGATGTGGCCACCATTGAGAATGCTCAGACCTATAACAATAATCTTATTAAACATGTATTATCGAATGCAGGAACACCAAAGAGGCCAGGAATGAGCATCGACACATA

Ace file of the UniGene sp_v3.0_unigene35248

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Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pollen allergen CJP38 n=1 Tax=Cryptomeria japonica RepID=Q588B8_CRYJA	-	-	76%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	70%
Sma3	Beta-1,3-glucanase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glucan endo-1,3-beta-D-glucosidase.	EC:3.2.1.39	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Starch and sucrose metabolism	00500		0.0	%

Source	Gene names
Sma3	At1g33220; At2g01630; At3g57260; At3g57270; At5g20330; At5g20340; B1142C05.26; BG2; BG2-1; BG2-2; BG2-3; BGLUC; F28O9.110; F28O9.120; GGL1; GLUB1; GLUB2; GLUB3; GN1; GN2; GNS1; GSVIVT00002713001; GSVIVT00015734001; GSVIVT00022220001; GSVIVT00022221001; GS

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	proteinaceous extracellular matrix	GO:0005578	Cellular Component	-
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	anchored to plasma membrane	GO:0046658	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	glucan exo-1,3-beta-glucosidase activity	GO:0004338	Molecular Function	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	cellulase activity	GO:0008810	Molecular Function	-
Sma3	licheninase activity	GO:0042972	Molecular Function	-
Sma3	glucan endo-1,3-beta-D-glucosidase activity	GO:0042973	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	defense response	GO:0006952	Biological Process	-
Sma3	response to cold	GO:0009409	Biological Process	-
Sma3	response to biotic stimulus	GO:0009607	Biological Process	-
Sma3	plant-type hypersensitive response	GO:0009626	Biological Process	-
Sma3	systemic acquired resistance	GO:0009627	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	Glycoside hydrolase, family 17	IPR000490	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	X8	IPR012946	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-

Source	Species	ID	Description	e value	Identity
ATG	Arabidoptis thaliana	AT3G57270.1	BG1 beta-1,3-glucanase 1 chr3:21191336-21193118 REVERSE LENGTH=340	3.0e-38	51%
RefSeq	Arabidopsis thaliana	NP_191286.1	beta-1,3-glucanase 1 [Arabidopsis thaliana]	4.0e-38	51%
RefSeq	Populus trichocarpa	XP_002299791.1	predicted protein [Populus trichocarpa]	2.0e-38	50%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: A9NTI6

Fln msg: Overlapping hits, possible frame ERROR between 205 and 204, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 36 aas, your sequence is shorter than subject: 191 - 344

Fln protein: SIRTVVH*MPSTQYVSYLVPAMNNIQTAIQNANLQNIKVSTPHAFNVIGVSYPPSHGAFSDDVKDTMxxILKFLSDNGAPFMANVYPYLSYAGDTNGIHLDYALFQPTATPVTDKDHSYSNLFDAMVDTLLWAMEASGYPNIPIVIAESGWPSAGADVATIENAQTYNNNLIKHVLSNAGTPKRPGMSIDT

Protein Length: 192

Fln nts: TTCGATCCGGACGGTGGTGCATTGAATGCCTAGCACGCAGTACGTATCGTATCTTGTTCCTGCCATGAACAACATTCAAACCGCGATCCAGAACGCCAATCTGCAGAACATCAAGGTCTCTACCCCCCATGCCTTCAATGTTATAGGCGTCAGTTACCCGCCTTCACATGGAGCATTCAGCGACGATGTGAAGGATACCATGxxxxxxATACTCAAATTTCTTTCAGATAACGGGGCTCCGTTCATGGCCAATGTGTATCCATATTTGAGTTACGCCGGCGACACCAACGGCATTCATCTGGACTACGCTCTCTTTCAGCCCACGGCTACGCCGGTGACAGACAAAGATCACAGCTACAGCAACCTGTTCGATGCCATGGTTGATACTCTTTTGTGGGCCATGGAAGCCTCGGGGTATCCCAACATCCCGATCGTCATTGCCGAAAGTGGATGGCCTTCTGCTGGCGCGGATGTGGCCACCATTGAGAATGCTCAGACCTATAACAATAATCTTATTAAACATGTATTATCGAATGCAGGAACACCAAAGAGGCCAGGAATGAGCATCGACACATA

Fln Alignment:

Contig35248___MPSTQYVSYLVPAMNNIQTAIQNANLQ-NIKVSTPHAFNVIGVSYPPSHGAFSDDVKDTMSAILKFLSDNGAPFMANVYPYLSYAGDTNGIHLDYALFQPTATPVTDKDHSYSNLFDAMVDTLLWAMEASGYPNIPIVIAESGWPSAGADVATIENAQTYNNNLIKHVLSNAGTPKRPGMSIDT
A9NTI6_______________LPSTQYVSYLFPAMTNIQTAVQNANLQNNIKVSTTHVMGVTN-GFPPSQGVFGDDVKDTMNSILKFLSDNGAPYMANVYPYFSYTGSGGSITLDYALFKSTSTVVTDNGRSYTNLFDAMVDTVISAMENLGYPNVPIVITESGWPSAGADAATVENAQSYNNNLIQHILSNAGTPKRSGTSIET

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
359		33	66		1.0
395		40	60		1.0
414		25	75		0.996473
420		50		50	1.0
439	50		50		1.0
473		50		50	1.0
501		25		75	0.996473
559	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain