SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene33053

Length: 1326 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene33053 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Caffeic acid O-methyltransferase (Fragment) n=1 Tax=Pinus pinaster RepID=Q9FEU5_PINPS	-	-	0.0	76%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	81%
Sma3	Caffeic acid O-methyltransferase	-	-	1.608e-08	-

Source	ECs	Term	Type	e value	Identity
Sma3	(RS)-norcoclaurine 6-O-methyltransferase.	EC:2.1.1.128	-	3.773e-07	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Isoquinoline alkaloid biosynthesis	00950		3.773e-07	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063		3.773e-07	%
Sma3	Metabolic pathways	01100		3.773e-07	%
Sma3	Biosynthesis of secondary metabolites	01110		3.773e-07	%
Sma3	(Iso)eugenol O-methyltransferase.	EC:2.1.1.146	-	6.352e-06	-
Sma3	Catechol O-methyltransferase.	EC:2.1.1.6	-	4.295e-06	-

Source	KEGGs	Term	e value	Identity
Sma3	Steroid hormone biosynthesis	00140	4.295e-06	%
Sma3	Tyrosine metabolism	00350	4.295e-06	%
Sma3	Betalain biosynthesis	00965	4.295e-06	%
Sma3	Metabolic pathways	01100	4.295e-06	%

Source	Gene names
Sma3	AOMT1; AOMT3; COMT; COMT-1; COMT1; COMT4; COMTL3; FOMTL1; GSVIVT00011171001; GSVIVT00018655001; GSVIVT00018658001; GSVIVT00025990001; GSVIVT00026179001; GSVIVT00036190001; LOC_Os12g13800; MtrDRAFT_AC150443g26v2; OMT I-a; OMT I-b; OMT1; OMT2; OsJ_35689; PO

Source	GOs	Term	Type	Identity
Sma3	O-methyltransferase activity	GO:0008171	Molecular Function	-
Sma3	catechol O-methyltransferase activity	GO:0016206	Molecular Function	-
Sma3	(RS)-norcoclaurine 6-O-methyltransferase activity	GO:0030786	Molecular Function	-
Sma3	protein dimerization activity	GO:0046983	Molecular Function	-
Sma3	caffeate O-methyltransferase activity	GO:0047763	Molecular Function	-
Sma3	lignin biosynthetic process	GO:0009809	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	O-methyltransferase, family 2	IPR001077	-	-
Sma3	Winged helix-turn-helix transcription repressor DNA-binding	IPR011991	-	-
Sma3	Plant methyltransferase dimerisation	IPR012967	-	-
Sma3	O-methyltransferase, COMT, eukaryota	IPR016461	-	-

Source	Species	ID	Description	e value	Identity
RefSeq	Populus trichocarpa	XP_002329411.1	alkaloid o-methyltransferase related [Populus trichocarpa]	0.0	46%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NU35

Fln msg: Separated hits, possible frame ERROR between 743 and 754,

Fln protein: MLTLAPPMDSIDHVLARHIDSSQESAVTQELQLYEMIFSAVKPLALKAAVLLNIPDIIATRGNGGPLSAEQIAFHIAAANSSSTGNSHVDVGYLYRILRFLASYGVFAEQEEADQPDDADTKKIKFGLTGISKLLVQEGNQQSCGPFLLLMANNVYLEAFQHLHESVLEGCYTFNKAYGTSPWEYLGRDPQANQIFNNAMAANSSTVMASVAKMYEDGFKSMSTLVDVGGGMGSALSIIVKxxxxxVRGINLDLPHVIATASPITGVEHMEGNMFEHIPSADAVMMKWIIHDWADDECV*LLRRSYEATPVKGKVIIVEAIVGGDKEGESISRRLGLLYDILLMVYTTGGKERTEEEFKGLFERAGFKRYTIIKFPFLQSLIVLSKS*

Protein Length: 389

Fln nts: ATGTGACGAGCCAAAAC_-_ATGCTCACCCTCGCACCTCCTATGGACTCCATTGATCATGTTTTGGCTCGTCACATTGACTCATCACAGGAATCCGCAGTAACGCAGGAGCTCCAGCTGTATGAGATGATATTCTCAGCTGTCAAGCCATTGGCTTTGAAAGCAGCCGTGTTGCTCAACATTCCTGACATCATCGCCACACGAGGAAACGGAGGTCCGCTTTCTGCCGAGCAGATTGCCTTTCATATTGCTGCTGCTAATTCCTCATCAACTGGAAATTCCCACGTGGATGTGGGATACCTCTACAGAATTCTGAGGTTCCTTGCTTCCTATGGCGTTTTCGCTGAACAAGAAGAGGCCGATCAGCCAGACGATGCTGACACCAAGAAAATAAAGTTCGGTTTGACAGGCATTTCGAAGTTGCTGGTGCAGGAAGGAAATCAGCAATCATGTGGGCCGTTCCTGCTCCTGATGGCAAACAACGTCTACCTGGAAGCCTTTCAACATTTGCACGAATCTGTCTTGGAGGGCTGCTACACATTTAACAAAGCTTATGGTACGAGTCCCTGGGAATATCTGGGCCGCGATCCCCAAGCTAATCAGATTTTCAACAACGCCATGGCCGCTAATAGCAGCACTGTCATGGCTTCCGTGGCGAAGATGTATGAAGATGGGTTTAAGAGTATGTCGACGCTTGTAGACGTTGGAGGAGGTATGGGCTCTGCATTATCCATCATTGTGAAAGAxxxxxxxxxxxxAGTTCGGGGGATTAATCTCGATCTGCCTCATGTCATTGCCACTGCGTCTCCCATAACTGGGGTGGAGCATATGGAGGGAAATATGTTCGAGCACATACCTTCTGCCGATGCAGTCATGATGAAGTGGATTATCCATGACTGGGCGGACGATGAGTGTGTGTAATTGCTGAGAAGAAGCTACGAGGCAACGCCAGTGAAGGGAAAGGTGATAATTGTGGAAGCCATTGTCGGTGGAGACAAAGAAGGTGAAAGCATATCGAGGCGATTGGGATTGTTATATGATATATTGCTGATGGTTTATACAACTGGTGGGAAGGAGAGAACAGAGGAAGAATTCAAAGGACTGTTCGAGCGGGCAGGGTTCAAGAGGTACACCATCATCAAGTTCCCTTTCCTTCAGTCGCTCATAGTGCTGTCCAAATCCTAA___TAAGCTGTTGCGCTCCAGATTATCGTTACAGTAATCTGATCTATGCAGTTTTGCTGGGATTGTTATCATGCAGTATATGATCTATGTTTTATGTGCTATGTCAACAGTTTGTTAACATGCAGTATATGATCTATGTTTTATGTGACG

Fln Alignment:

Contig33053___MDSIDHVLAR----HIDSSQESAVTQELQLYEMIFSXXXXXXXXXXXXLNIPDIIATRGNGGPLSAEQIAFHIAAANSSSTGNSHVDVGYLYRILRFLASYGVFXXXXXXXXXXXXDTKKIKFGLTGISKLLVQEGNQQSCGPFLLLMANNVYLEAFQHLHESVLEGCYTFNKAYGTSPWEYLGRDPQANQIFNNAMAANSSTVMASVAKMYEDGFKSMSTLVDVGGGMGSALSIIVKExxxxVRGINLDLPHVIATASPITGVEHMEGNMFEHIPSADAVMMKWIIHDWADDECV*LLRRSYEATPVKGKVIIVEAIVGGDKEGESISRRLGLLYDILLMVYTTGGKERTEEEFKGLFERAGFKRYTIIKFPFLQSLIVLSKS
A9NU35_______________MESIDRVFSRGTGQQMISSCESAATQELQLYEMMFSAVKPMALKAAVLLKIPDIIAAQGNGGPLSVEQIASHIAAANSSSTSNSHVDVGYLYRILRFLASYGVFTEQEEADQADDADTKKIKYGLTAISKLLAQSENQQSCGPFLFLMAEKVYLEAYQHLHESVLEGCYTFNKAYDMSPWEYLSHDPQANRIFNEAMTANSSTVMASVAKMYEDGFESINTLVDVGGGMGSSLSMIVKExxxxIRGINFDLPHVIATAPPITGVEHMEGNMFEHIPSADAVMMKWILHDWDDEKCVKLLRRSYEATPANGKVLIVDAVVGGGKEAESMSRRLGLLFDIAMMVYTTGGKERTEEEFKRLFQTAGFRSYRIIKLPFLQALIVLSKS

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
590		25		75	0.996473
595	25	75			0.996473
605		25		75	0.996473
608			25	75	0.996473
609			75	25	0.996473
638	28	71			1.0
644		71		28	1.0
646	28	71			1.0
687		14	85		0.993533
719	33		66		1.0
721		16		83	0.994512
728	66		33		1.0
733			66	33	1.0
734	66		33		1.0
742			50	33	1.0
746	83			16	0.994512
755		66	33		1.0
770	80		20		0.995493
774	20		80		0.995493
777		80		20	0.995493
1190		33	66		0.997452
1196	33			66	0.997452
1235	66		33		0.997452
1247		33		66	0.997452
1258	66		33		0.997452
1272	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain