SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene33029

Length: 1448 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene33029 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	subtilisin-like proteinase-like [Oryza sativa Japonica Group] dbj\|BAB90087.1\| subtilisin-like proteinase-like [Oryza sativa Japonica Group]	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	45%
Sma3	Subtilisin-like protease	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	2-alkenal reductase.	EC:1.3.1.74	-	1.536e-12	-
Sma3	Cucumisin.	EC:3.4.21.25	-	1.494e-36	-

Source	Gene names
Sma3	46C02.13; AIR3; ARA12; ASP48; AT3G14067; AT4g34980; At1g01900; At1g04110; At1g20160; At2g04160; At2g05920; At3g14067; At3g14240; At4g34980; At5g45650; At5g45650/MRA19_5; At5g51750; At5g59810/mmn10_30; At5g67360; F20D22.12; F22M8.3; GSVIVT00000486001; GSVI

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	external side of plasma membrane	GO:0009897	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	extracellular matrix	GO:0031012	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	GMP synthase (glutamine-hydrolyzing) activity	GO:0003922	Molecular Function	-
Sma3	serine-type endopeptidase activity	GO:0004252	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	identical protein binding	GO:0042802	Molecular Function	-
Sma3	GMP biosynthetic process	GO:0006177	Biological Process	-
Sma3	proteolysis	GO:0006508	Biological Process	-
Sma3	glutamine metabolic process	GO:0006541	Biological Process	-
Sma3	response to auxin stimulus	GO:0009733	Biological Process	-
Sma3	plant-type cell wall modification	GO:0009827	Biological Process	-
Sma3	lateral root morphogenesis	GO:0010102	Biological Process	-
Sma3	stomatal complex morphogenesis	GO:0010103	Biological Process	-
Sma3	regulation of cell proliferation	GO:0042127	Biological Process	-
Sma3	negative regulation of catalytic activity	GO:0043086	Biological Process	-
Sma3	mucilage metabolic process involved seed coat development	GO:0048359	Biological Process	-
Sma3	mucilage extrusion from seed coat	GO:0080001	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	C2 calcium-dependent membrane targeting	IPR000008	-	-
Sma3	Phosphofructokinase domain	IPR000023	-	-
Sma3	Ornithine/DAP/Arg decarboxylase	IPR000183	-	-
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3		IPR000991	-	-
Sma3	Arf GTPase activating protein	IPR001164	-	-
Sma3	GMP synthase, C-terminal	IPR001674	-	-
Sma3	Protease-associated domain, PA	IPR003137	-	-
Sma3	GMP synthase, N-terminal	IPR004739	-	-
Sma3	Domain of unknown function DUF591	IPR007649	-	-
Sma3	Proteinase inhibitor I9	IPR010259	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3		IPR011702	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	Peroxisome membrane protein, Pex16	IPR013919	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-
Sma3	Peptidase S8, subtilisin-related	IPR015500	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Glutamine amidotransferase type 1	IPR017926	-	-
Sma3	C2 membrane targeting protein	IPR018029	-	-
Sma3	Chromogranin, conserved site	IPR018054	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-
Sma3	Peptidase S10, serine carboxypeptidase, active site	IPR018202	-	-
Sma3		IPR018318	-	-
Sma3	Peroxidases heam-ligand binding site	IPR019793	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G67360.1	ARA12 Subtilase family protein chr5:26872192-26874465 REVERSE LENGTH=757	50%
RefSeq	Arabidopsis thaliana	NP_569048.1	subtilisin-like protease [Arabidopsis thaliana]	50%
RefSeq	Populus trichocarpa	XP_002317314.1	predicted protein [Populus trichocarpa]	54%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AAS9

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 336 - 394

Fln protein: FTS*AGGAAIILGNLPINGAEISVDAYVLPGTAVVSNDAATILAYITSTTKPRAHIVPAHTVLGTRPAPFMAAFSSRGPNLVNPDILKPDITAPGLNILAAWSGAASPTKLLMDDRRVKYNIISGTSMSCPHIAGIAALLKAIHPNWSPAAIKSALMTTANVTNNEGHPISDASGNPATPFSYGSGQVNPNQASDPGLLYDATIEDYVLFLCGSGANASTILRNRNFKCPKNPPKDYQLNYPSVAIANLINKETVTRTVTNVGGKGEYTVSVDEPPGVSVHINPKKLSFKSTGEKQIFTIQFTLQKPLNRKYVFGSYTWSDGNHLVRSPIAVSSM*

Protein Length: 337

Fln nts: TTTCACCTCTTAGGCAGGAGGGGCTGCAATCATTCTGGGGAATCTGCCAATTAATGGGGCAGAAATATCAGTGGATGCATATGTTCTACCTGGGACTGCAGTGGTCTCTAATGATGCAGCAACCATACTTGCCTACATCACTTCTACTACTAAACCCCGTGCACATATTGTTCCAGCCCACACTGTCCTGGGAACCAGGCCAGCACCATTCATGGCCGCCTTCTCTTCCAGGGGCCCCAATTTGGTCAATCCAGACATCCTGAAGCCAGATATTACAGCACCTGGTCTGAATATACTGGCAGCCTGGAGTGGAGCAGCCTCTCCTACCAAGCTTTTAATGGATGATCGTCGTGTAAAGTACAATATTATCTCCGGCACATCAATGTCGTGCCCACATATTGCAGGAATAGCAGCCCTGTTAAAGGCAATTCATCCAAATTGGAGCCCTGCCGCTATCAAATCTGCCCTCATGACCACTGCAAATGTTACAAACAATGAAGGTCATCCAATTTCAGATGCATCTGGAAATCCAGCCACGCCATTCAGTTATGGATCAGGTCAAGTTAACCCCAATCAGGCATCAGATCCAGGCCTGTTGTATGATGCCACAATAGAAGATTACGTCCTCTTTCTTTGTGGCAGTGGAGCTAATGCTTCTACCATTTTACGAAACAGAAATTTCAAATGCCCGAAAAATCCACCTAAAGATTATCAACTGAATTATCCATCAGTTGCCATTGCAAACTTGATTAACAAGGAGACGGTCACTAGGACTGTCACCAATGTTGGTGGCAAAGGCGAATACACTGTTTCAGTGGATGAACCACCAGGTGTATCTGTACATATCAATCCCAAGAAGTTGTCTTTCAAATCTACAGGAGAAAAACAGATCTTCACTATACAATTCACCCTTCAAAAGCCTCTAAATAGAAAGTATGTATTTGGGTCCTATACTTGGAGTGATGGAAATCATCTAGTCAGGAGTCCCATAGCTGTCTCATCAATGTAA___ATAGGCATACTCAGCCATTCATCTCATGGATCACTAAAATGGATGAACTTGAGGAACGAGAATCTTTATGCTTTAAGAATGTGAGTGGCTTAGATCCATGTCTGCTCAGGCTCCATACAAATTGGCTTCCAATGAGCTGGAGTGCTTGTAAGTTGTAGCTGTATGTTCAAGCATAGTTACATCCTCTGCTCAGGTTAGATTTCTGTGCATTAGATTGACAGAGAAGCTCTTTCAACTCAAATCTCGGTGTACAAGCAAATGCATTCAATACAAAGCAAATTTCGTGCGTCATTTGAATGGCCCGAAAATGATAAAGATATATGGGTTTAGCCTAGTTCAACGATTAAGAAGCTAAAGAGCCCTGTTTTGTAACATGGTCATCTCTCTTTGAGACAGTAAGTATAATAAATTCAAGATAACTGAGAATTATTCAGACTTT

Fln Alignment:

Contig33029___AGGAAIILGNLPINGAEISVDAYVLPGTAVVSNDAATILAYITSTTKPRAHIVPAHTVLGTRPAPFMAAFSSRGPNLVNPDILKPDITAPGLNILAAWSGAASPTKLLMDDRRVKYNIISGTSMSCPHIAGIAALLKAIHPNWSPAAIKSALMTTANVTNNEGH-PISDASGNPATPFSYGSGQVNPNQASDPGLLYDATIEDYVLFLCGSG--ANASTILRNRNFKCPKNPPKDYQLNYPSV-AIANLINKETVT----RTVTNVGGK-GEYTVSVDEP-PGVSVHINPKKLSFKSTGEKQIFTIQFTLQK----PLNRKYVFGSYTWSDGNHLVRSPIAV
D5AAS9_______________AGGVGMILANSDSDGEGLVADAHMLPTSAVGAKEGELIRQYIATTKGPTATISGLGTVLGIKPAPVVASFSSRGPNPETPEILKPDVIAPGVNILAGWTGAVGPSSLAIDRRRTQFNILSGTSMACPHVSGVAALLKGAHPQWSPAAVRSALMTTAYTQDNRGHRMIDEASGNASTPFDFGAGHVHPERAMDPGLIYDMDTEDYVRFLCSLNYTSKAIQVITRKPTRCPARRISPGNINYPSFSAVFDLTQPKKLSTVFFRTVTNVGPPLSVYRVRVIHPRGGVTVTVKPRKLVFTEKNQKLSYTVTVTTKHIDLLPGNADTRFCFISWTDGKHVVQSPITI

SNPs (tot: 40)

Position	A %	C %	G %	T %	Probability
150			25	75	0.996473
152	75			25	0.996473
202		12		87	0.992554
243	83		16		0.994512
265	33	66			1.0
291		33	66		1.0
303		83		16	0.994512
351	40			60	1.0
358	20	80			0.995493
385		50		50	1.0
386	50		50		1.0
413		50		50	1.0
625		16	83		0.994512
635		83	16		0.994512
653		16	83		0.994512
683	71		28		1.0
689		71	28		1.0
702	71	28			1.0
731	71		28		1.0
803	77			22	0.999999
827			87	12	0.992554
830	12		87		0.992554
984	90		10		0.90298
1001			70	30	1.0
1029	12			87	0.992554
1079		20	80		0.999996
1097		22		77	0.999999
1154		87		12	0.992554
1160	77		22		0.999999
1221		66		33	1.0
1229	22		77		0.999999
1255		11		88	0.923564
1256		11	88		0.923564
1257		11	88		0.923564
1258	11			88	0.923564
1310	66		33		1.0
1341		20		80	0.995493
1342		20		80	0.995493
1345			80	20	0.995493
1395		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain