SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene32299

Length: 1033 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene32299

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Caffeoyl-CoA O-methyltransferase n=8 Tax=Pinaceae RepID=CAMT_PINTA	-	-	75%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	88%
Sma3	Caffeoyl-CoA O-methyltransferase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Caffeoyl-CoA O-methyltransferase.	EC:2.1.1.104	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Phenylalanine metabolism	00360	%
Sma3	Phenylpropanoid biosynthesis	00940	%
Sma3	Flavonoid biosynthesis	00941	%
Sma3	Stilbenoid, diarylheptanoid and gingerol biosynthesis	00945	%
Sma3	Biosynthesis of phenylpropanoids	01061	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	AsCCOAOMT; At1g24735; At1g67980; At1g67990; At3g61990; At4g26220; At4g34050; CAM1; CAM2; CAML2; CAML3; CCOAOMT; CCOAOMT1; CCOAOMT2; CCOAOMT3; CCOAOMT4; CCOAOMT5; CCOAOMT6; CCOMT; CCoAMT; CCoAOMT; CCoAOMT1; CCoAOMT2; CCoAOMTI; COA1; COA20; COA26; CtCoAOMT1

Source	GOs	Term	Type	Identity
Sma3	magnesium ion binding	GO:0000287	Molecular Function	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	O-methyltransferase activity	GO:0008171	Molecular Function	-
Sma3	caffeoyl-CoA O-methyltransferase activity	GO:0042409	Molecular Function	-
Sma3	metal ion binding	GO:0046872	Molecular Function	-
Sma3	trihydroxyferuloyl spermidine O-methyltransferase activity	GO:0080012	Molecular Function	-
Sma3	response to stress	GO:0006950	Biological Process	-
Sma3	coumarin biosynthetic process	GO:0009805	Biological Process	-
Sma3	lignin biosynthetic process	GO:0009809	Biological Process	-
Sma3	DNA integration	GO:0015074	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	seed development	GO:0048316	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	O-methyltransferase, family 3	IPR002935	-	-
Sma3	Domain of unknown function DUF292, eukaryotic	IPR005061	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G34050.1	CCoAOMT1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein chr4:16310844-16311973 FORWARD LENGTH=259	71%
RefSeq	Arabidopsis thaliana	NP_195131.1	putative caffeoyl-CoA O-methyltransferase [Arabidopsis thaliana]	71%
RefSeq	Populus trichocarpa	XP_002313125.1	trans-caffeoyl-coa 3-o-methyltransferase [Populus trichocarpa]	71%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD15

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 235 - 251

Fln protein: MEHKMGHKGILQSDALYQYILETSVYPREPEPLKELRQATAKVPWNFMAIPPDEGQFLSLLLKLMNAKNTIEIGVYTGYSLLSTALALPDDGKIIAMDPDRATYEIGLPIIEKAGVAHKIDFREGPALPFLDEMIKNVGMHGSFDFAFVDADKANYLNYHERLIDLVKIGGVIAYDNTLWKGSVAAPPEDEMNEGFRKVRVFTLEVNKVLAADPRVEIAQIPVADGITLCRRLS*

Protein Length: 236

Fln nts: AACAATGGCAATATTTTCTTCTTGTTCTCGCATTTGTGGATCTGCTATGGTTTTCGGTCAGCTATAGTAGAGCTGAAGGCGGACACATCCCAAGTACAGGAGCAGAGTAAGGTCACAAA_-_ATGGAACATAAAATGGGCCATAAAGGTATTTTGCAGAGTGATGCGCTTTATCAGTATATATTGGAAACGAGCGTGTATCCTCGTGAACCCGAGCCACTGAAAGAGCTTCGGCAGGCGACTGCAAAAGTTCCCTGGAACTTTATGGCCATTCCTCCCGATGAGGGTCAATTTCTGAGCCTTCTGCTGAAGCTAATGAATGCCAAGAACACCATCGAGATTGGGGTGTACACCGGGTACTCACTTCTGAGCACAGCCCTTGCTTTACCTGATGACGGGAAGATAATAGCGATGGATCCTGATAGAGCGACTTATGAGATCGGGTTACCTATCATTGAAAAAGCTGGAGTTGCCCACAAAATTGACTTCAGAGAGGGCCCTGCTCTGCCATTTTTGGATGAAATGATTAAAAATGTTGGGATGCACGGCTCGTTTGATTTTGCGTTTGTGGACGCCGACAAAGCGAACTATCTGAACTACCATGAGCGGCTGATCGATCTGGTGAAGATCGGGGGTGTGATTGCGTATGACAATACTCTGTGGAAAGGAAGCGTTGCGGCTCCCCCCGAAGATGAGATGAACGAAGGGTTCAGAAAAGTGAGAGTTTTCACATTGGAAGTGAACAAGGTGCTTGCCGCTGATCCCCGCGTCGAGATTGCCCAAATCCCAGTCGCCGATGGAATCACGCTGTGCAGGCGTCTCTCCTGAAGTTTATTCAGCGATCAGTTCACAGTATGCTGTGGATGTGAGCCCTGACAGTCAGAAATTATATCCATAGAAATTGTTTCTAAATTTTGTGGTATAATTTGTTTGCTAAATTAGTCGGTGTGGTATTTAATGTAATATTTATTAGGATATTTGACAGCAAATAGGCCTTAGTGCCGGAGGAAATAAAGAATTTGAAAACCAGTGCTATT

Fln Alignment:

Contig32299___MEHKMGHKGILQSDALYQYILETSVYPREPEPLKELRQATAKVPWNFMAIPPDEGQFLSLLLKLMNAKNTIEIGVYTGYSLLSTALALPDDGKIIAMDPDRATYEIGLPIIEKAGVAHKIDFREGPALPFLDEMIKNVGMHGSFDFAFVDADKANYLNYHERLIDLVKIGGVIAYDNTLWKGSVAAPPEDEMNEGFRKVRVFTLEVNKVLAADPRVEIAQIPVADGITLCRRLS
D5AD15_______________LDHQMDNKSLLQSDALYQYILETNVYPREPEPLKELRQVTAKVPWNFMAIPPDEGQFLSLLLKLMNAKNTIEIGVYTGYSLLSTALALPEDGKIIAMDTDRRTYEIGRPIIEKAGVAHKIDFREGPALPFLDEMLKNGGMQGSFDFAFVDADKGNYLNYHKRLIDLVKIGGVIAYDNTLWKGTVASPPEVHMNEGFRNVRLFTLEVNKALAADPRIEIAQIPVADGITLCRRLS

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
288			40	60	1.0
441		66		33	0.997452
505		40		60	1.0
517	85		14		0.993533
676	10	90			0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain