SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene31876

Length: 1998 nt

UniGene Fasta

>sp_v3.0_unigene31876
ACGGAGAGCCTTTGTATTTATATAGATCCAAGCTTCCCGATATTGAAATCTCCAGCCATCTTACTCTGCACGATTATTGCTTCGAGAAATTGGGGGAATTTGCAGAGAGGCCATGCATTGTACAGGGATCTAATGGGAAAGTTTATTCCTATGGCGAAGTTGATTTGATTTCTCGCAGGGTCGGCTCTGGTTTGGCCAAGCTCGGCATCAGGCATGGAGATGTCGTTATGCTTTTGCTGCCCAATTGCCCAGAGTTCGCTTTCGTTTTCTTGGGTTCGTCTCGAATTGGCGCCATTGCGACAACTGCCAATCCTTTCTATACGCCGGGCGAGATTGCGAAGCAGGCAATGGCGTCCCAGGCTCGGGTGATCGTCACTCAGGCTACGTATGTCGAGAAATTGTGCAATCTCGAGAGGGAAAAAAGTTCAATTCTGGTTATTACAATTGACCCAGGGGCTCCGGAGGGGTGCCTGCATTTCTCGGTTCTGGCAGAGGCCGACGAGAATGAGATACCCGTTGTCGAGATTCGACCTGACGACGTCATGGCTTTGCCCTATTCCTCTGGCACCACCGGGCTTCCCAAGGGCGTCATGCTCACGCACGGGGCGCTCGTTTCTTCTGTTGCGCAGCAGGTGGACGGCGAGAATCCCAATCTGTATCTGCATTGCGAGGACGTGGTTCTGTGCGTGCTGCCGCTGTTTCATATTTATTCTCTCAATTCTGTGCTGCTCTGTTCTCTCAGAGCGGGCGCGGCTGTGTTGATTATGCAGAGATTCGAAATCCATGCGATGCTGGAGCTGATACAGAAATTCAAGGTCACTGTGGCTCCGATCGTGCCTCCGATTGTTCTGCCCATCGCCAAGAATCCAGTAGTAGAAAAATATGATATGTCGTCCATCAGAATGGTCTTGTCAGGCGCTGCGCCTCTCGGGAAGGAGCTGGAAGACGCCCTTAGGGCTAGGATTCCCAACGCAACTTTGGCCCAGGGTTATGGTATGACAGAGGCTGGACCAGTTCTGGCCATGTCTCTGGGTTTCGCAAAAGTGCCCTTTACTATAAAGTCTGGCTCCTGCGGTACCGTGGTTAGAAATGCGGAGATGAAGATAATCGAACCCGAGACCGGAGTCTCCCTGCCACATAACCACCGGGGAGAGATCTGTATCCGCGGCCCTCAAATTATGAAAGGGTATCTGAATGATCCGGAGGCGACTGAGATGACGATTGATAAAGATGGATGGCTGCATACCGGAGACGTTGGTTACATTGACGATGATGAAGAGCTTTTCATAGCGGACCGTGTCAAGGAACTGATCAAATACAAAGGGTTCCAGGTGCCACCGGCGGAACTGGAAGCGATTCTTTTGAGCCATCCATCCATAGCCGACGCAGCAGTTGTGTCGCAGCAGGATGAAATTGCTGGAGAGGTGCCGGTAGCCTTTGTGGTGAGATCTGGTGATGGGATTGATTTAACGGTGAATGAGATTAAAGAATTCGTTGCCAAACAGGTGGTGTTCTACAAGAAGCTGCACAACGTTTATTTTATAGAAGCCATACCCAAATCTCCATCTGGCAAGATACTGCGCAAGGATTTAAGAGCCAAGCTTATGGCTGTCGCTGCAGGCATTCAATAATTTCAGCGGCCATGGCAGAAGGAGCCTTATCTGCTTTTATATTAAAATATATACAGATTTTATGTATTTTCTGGTGTCTCGCCAGCAGGATTGACCTGGTAGTGTAAGTTGTGAAAGTTTACTTATTCATTTATTTCCTCGTTCATTTTTAGCTGGCCTGAGATGACTCTGGACATGTTTCTAATTATAGTGTTGCTGCTGCCATGGTATGCATGGCATGACATGACAGAGTTGTTGTCAGTTTATTCCATTTTTAATATTGGGTAAACGGCCTTTGGTTGTTGGTAACACATTCATCGTGTGACGATGAATGGCAAATCAGTGTGACTCAGCTCATGTAATACTGTTTTTTATAAATGTTGGTTCC

Ace file of the UniGene sp_v3.0_unigene31876

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	4-coumarate--CoA ligase n=18 Tax=Pinaceae RepID=4CL_PINTA	-	-	70%
FL-Next	tr=4-coumarate:CoA ligase; Pinus massoniana.	-	-	73%
Sma3	4-coumarate:CoA ligase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing).	EC:1.13.12.7	-	2.393e-21	-
Sma3	4-coumarate--CoA ligase.	EC:6.2.1.12	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Ubiquinone and other terpenoid-quinone biosynthesis	00130	%
Sma3	Phenylalanine metabolism	00360	%
Sma3	Phenylpropanoid biosynthesis	00940	%
Sma3	Biosynthesis of phenylpropanoids	01061	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	4CL; 4CL-1; 4CL-2; 4CL-2a; 4CL-2b; 4CL.2; 4CL1; 4CL2; 4CL3; 4CL4; 4CL5; 4CLL1; 4CLL7; 4cl; 4cl1; 4cl2; ACLL13; ACLL14; ACLL18\|Ptr4CL13; ACLL20; ACLL21; At1g51680; At1g62940; At1g65060; At3g21230; At3g21240; At4g05160; C17L7.80; Egl4CL; F16G16.6; F16P17.9;

Source	GOs	Term	Type	Identity
Sma3	peroxisome	GO:0005777	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	fatty-acyl-CoA synthase activity	GO:0004321	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	4-coumarate-CoA ligase activity	GO:0016207	Molecular Function	-
Sma3	ligase activity	GO:0016874	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	jasmonic acid biosynthetic process	GO:0009695	Biological Process	-
Sma3	phenylpropanoid metabolic process	GO:0009698	Biological Process	-
Sma3	phenylpropanoid biosynthetic process	GO:0009699	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Regulator of chromosome condensation, RCC1	IPR000408	-	-
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	-
Sma3	Adenosine/AMP deaminase active site	IPR006650	-	-
Sma3	HTH domain AraC-type, conserved site	IPR018062	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G21240.1	4CL2, AT4CL2 4-coumarate:CoA ligase 2 chr3:7454497-7457314 REVERSE LENGTH=556	65%
RefSeq	Arabidopsis thaliana	NP_188761.1	4-coumarate--CoA ligase 2 [Arabidopsis thaliana]	65%
RefSeq	Populus trichocarpa	XP_002329649.1	4-Coumarate:CoA ligase [Populus trichocarpa]	68%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: C1KBY2

Fln msg: atg_distance in limit (1-15): atg_distance = 6, W2: There is no M at the beginning,

Fln protein: GEPLYLYRSKLPDIEISSHLTLHDYCFEKLGEFAERPCIVQGSNGKVYSYGEVDLISRRVGSGLAKLGIRHGDVVMLLLPNCPEFAFVFLGSSRIGAIATTANPFYTPGEIAKQAMASQARVIVTQATYVEKLCNLEREKSSILVITIDPGAPEGCLHFSVLAEADENEIPVVEIRPDDVMALPYSSGTTGLPKGVMLTHGALVSSVAQQVDGENPNLYLHCEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLIMQRFEIHAMLELIQKFKVTVAPIVPPIVLPIAKNPVVEKYDMSSIRMVLSGAAPLGKELEDALRARIPNATLAQGYGMTEAGPVLAMSLGFAKVPFTIKSGSCGTVVRNAEMKIIEPETGVSLPHNHRGEICIRGPQIMKGYLNDPEATEMTIDKDGWLHTGDVGYIDDDEELFIADRVKELIKYKGFQVPPAELEAILLSHPSIADAAVVSQQDEIAGEVPVAFVVRSGDGIDLTVNEIKEFVAKQVVFYKKLHNVYFIEAIPKSPSGKILRKDLRAKLMAVAAGIQ*

Protein Length: 544

Fln nts: ACGGAGAGCCTTTGTATTTATATAGATCCAAGCTTCCCGATATTGAAATCTCCAGCCATCTTACTCTGCACGATTATTGCTTCGAGAAATTGGGGGAATTTGCAGAGAGGCCATGCATTGTACAGGGATCTAATGGGAAAGTTTATTCCTATGGCGAAGTTGATTTGATTTCTCGCAGGGTCGGCTCTGGTTTGGCCAAGCTCGGCATCAGGCATGGAGATGTCGTTATGCTTTTGCTGCCCAATTGCCCAGAGTTCGCTTTCGTTTTCTTGGGTTCGTCTCGAATTGGCGCCATTGCGACAACTGCCAATCCTTTCTATACGCCGGGCGAGATTGCGAAGCAGGCAATGGCGTCCCAGGCTCGGGTGATCGTCACTCAGGCTACGTATGTCGAGAAATTGTGCAATCTCGAGAGGGAAAAAAGTTCAATTCTGGTTATTACAATTGACCCAGGGGCTCCGGAGGGGTGCCTGCATTTCTCGGTTCTGGCAGAGGCCGACGAGAATGAGATACCCGTTGTCGAGATTCGACCTGACGACGTCATGGCTTTGCCCTATTCCTCTGGCACCACCGGGCTTCCCAAGGGCGTCATGCTCACGCACGGGGCGCTCGTTTCTTCTGTTGCGCAGCAGGTGGACGGCGAGAATCCCAATCTGTATCTGCATTGCGAGGACGTGGTTCTGTGCGTGCTGCCGCTGTTTCATATTTATTCTCTCAATTCTGTGCTGCTCTGTTCTCTCAGAGCGGGCGCGGCTGTGTTGATTATGCAGAGATTCGAAATCCATGCGATGCTGGAGCTGATACAGAAATTCAAGGTCACTGTGGCTCCGATCGTGCCTCCGATTGTTCTGCCCATCGCCAAGAATCCAGTAGTAGAAAAATATGATATGTCGTCCATCAGAATGGTCTTGTCAGGCGCTGCGCCTCTCGGGAAGGAGCTGGAAGACGCCCTTAGGGCTAGGATTCCCAACGCAACTTTGGCCCAGGGTTATGGTATGACAGAGGCTGGACCAGTTCTGGCCATGTCTCTGGGTTTCGCAAAAGTGCCCTTTACTATAAAGTCTGGCTCCTGCGGTACCGTGGTTAGAAATGCGGAGATGAAGATAATCGAACCCGAGACCGGAGTCTCCCTGCCACATAACCACCGGGGAGAGATCTGTATCCGCGGCCCTCAAATTATGAAAGGGTATCTGAATGATCCGGAGGCGACTGAGATGACGATTGATAAAGATGGATGGCTGCATACCGGAGACGTTGGTTACATTGACGATGATGAAGAGCTTTTCATAGCGGACCGTGTCAAGGAACTGATCAAATACAAAGGGTTCCAGGTGCCACCGGCGGAACTGGAAGCGATTCTTTTGAGCCATCCATCCATAGCCGACGCAGCAGTTGTGTCGCAGCAGGATGAAATTGCTGGAGAGGTGCCGGTAGCCTTTGTGGTGAGATCTGGTGATGGGATTGATTTAACGGTGAATGAGATTAAAGAATTCGTTGCCAAACAGGTGGTGTTCTACAAGAAGCTGCACAACGTTTATTTTATAGAAGCCATACCCAAATCTCCATCTGGCAAGATACTGCGCAAGGATTTAAGAGCCAAGCTTATGGCTGTCGCTGCAGGCATTCAATAA___TTTCAGCGGCCATGGCAGAAGGAGCCTTATCTGCTTTTATATTAAAATATATACAGATTTTATGTATTTTCTGGTGTCTCGCCAGCAGGATTGACCTGGTAGTGTAAGTTGTGAAAGTTTACTTATTCATTTATTTCCTCGTTCATTTTTAGCTGGCCTGAGATGACTCTGGACATGTTTCTAATTATAGTGTTGCTGCTGCCATGGTATGCATGGCATGACATGACAGAGTTGTTGTCAGTTTATTCCATTTTTAATATTGGGTAAACGGCCTTTGGTTGTTGGTAACACATTCATCGTGTGACGATGAATGGCAAATCAGTGTGACTCAGCTCATGTAATACTGTTTTTTATAAATGTTGGTTCC

Fln Alignment:

Contig31876___YLYRSKLPDIEISSHLTLHDYCFEKLGEFAERPCIVQGSNGKVYSYGEVDLISRRVGSGLAKLGIRHGDVVMLLLPNCPEFAFVFLGSSRIGAIATTANPFYTPGEIAKQAMASQARVIVTQATYVEKLCNLEREKSSILVITIDPGAPEGCLHFSVLAEADENEIPVVEIRPDDVMALPYSSGTTGLPKGVMLTHGALVSSVAQQVDGENPNLYLHCEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLIMQRFEIHAMLELIQKFKXXXXXXXXXXXXXXAKNPVVEKYDMSSIRMVLSGAAPLGKELEDALRARIPNATLAQGYGMTEAGPVLAMSLGFAKVPFTIKSGSCGTVVRNAEMKIIEPETGVSLPHNHRGEICIRGPQIMKGYLNDPEATEMTIDKDGWLHTGDVGYIDDDEELFIADRVKELIKYKGFQVPPAELEAILLSHPSIADAAVVSQQDEIAGEVPVAFVVRSGDGIDLTVNEIKEFVAKQVVFYKKLHNVYFIEAIPKSPSGKILRKDLRAKLMA
C1KBY2_______________HLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYSFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSH--DVLVITIDDAPKEGCQHISVLTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSEDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEETGEVPVAFVVKSS---EISEQEIKEFVAKQVVFYKKIHRVYFVDAIPKSPSGKILRKDLRSRLAA

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
294	60		40		1.0
302	60			40	1.0
384	60			40	1.0
405	50		50		1.0
1505			66	33	0.997452
1507			33	66	0.997452
1510		25		75	0.996473
1513		25		75	0.996473
1514		75	25		0.996473
1519	75	25			0.996473
1520			75	25	0.996473
1521	75			25	0.996473
1522	75		25		0.996473
1530	75	25			0.996473
1651	85		14		0.993533

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain