SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene31189

Length: 2412 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene31189
GTTTCAATTACAGGAAAACCATGATTCTGTTCATGCGTGCCCCGGGTCTTTATTTGTTTGTCGAAATAGCTGCATCCGAATCAGCAGGAGGAGGGGGGCATAGCACTGGCAGGGGAAGTAGCATAGCAAGCAACCAGCCAGGGAATTATATCAATTAAGGTGAGGAGAATTTCACACCTTCCATCAAAGGCAAGAGAGATCGATCTTTCCTTACCGATCTAATAACAAGGCAGGCACGAAACAACCTTCAAATTCTTCCCGACCTTTAACTCCTTCCTGATCTTCTAGCTCTTTTCCCCAGACATTGGAAGAGTTTTATGCCATGCTTTATCGATGCTTGTTATACTATAAAGGCCGTGTTTGTGTATTTCCAATGTTCTATACCCTTCGTTGCCTCTAAATTGTCGTCGTCTCCGAGGATTGAGTATAGAAGGAACTGTCAGAAGCCTCTTCCAGAATTTAGCGGATTCGGTTTGACTCATTCTCGCCATTTAATTTGAAGGTTTGTTGCTTTCGGATTTCGCATTCTTGAGGTTCTTTCTCAGCAAGTCCATGGCGGGAAAATTGGACCAGTGAAGCAGCCGTGATTGTATTACTGCATATGTGTCTTCTGTATTCATGTAGTCAACACGTATCTGATTTTGACTATCTTCTACCAGTGGACAACTTTGTTAGTATGGTGCACTTTTGAAATGTGTAGTACAACAGGTTATATACTTGAAAACTATATATTAGATCACATATGCCTTCTGTATTGGCAGACAGGTAGAAGTAGCAGTTAATTGATAGAGGGTACATTGATTTATCCTTGAGTTCAATATATAGGTCCTTATCTGAGCAACGACACTTCAAGGGAAACTAAGTTGTGTGAACAAATGGTGACATATGAGCCAGATCCAGATGTTGTCCGTTGGGGTCTCCATCTTCTTGATGGAGATTCTTTTTCCAATACAGGTCATCGGATTAATGCCACTCAATTGAGTGATACTACTCTATATCGGGGGATTCAGATTATGGAACCTCAATTGCCAAGAGAGCCCACTAATGTAGAAAATGATGAGATCATTGCTCATGCTCTTCAAGAAGAATTATCTCATCTGGCAGCTGTGGAAGCATCAGGTGACCCACACCCTACTGAAGATCATTTACAGGCCTCAATTCTTGCACAGGATTGGCTTGGTCCAAGCCAGCGAATATCTAGTGAAGGTGCATGGCATGTTGAGGAACAACAAGAATCAAGTCATGAACAAGAGGTTGCAAGTCCGTGCTCAAGTCTGGAAAGAAGGTCTCAAGATGGTTCTGAGGATGTCTATACATCTCTTGAGCTATTGGATGAGTTTTCTGCTTTGGATGGGGAAGTTGGCAAAAGATTGACGCAGATGAACTCTGTGCCTCATATTCCTAGGATTAATGGAGACATTCCTACCATGGATGAAGCAACCACAGACCATCAAAGGCTGTTGGACAGGTTAACACTCTATGATTTGCGTGAACTTAAAATTCCTGGAGATGGAAACTGCCAGTTTCGAGCACTCTCTGATCAGTTTTATCGAACACCTGAGCATCATGAGTTTGTCAGGCAACAGGTTGTTGACCAGCTAAAATCTGAACCAGAATTATATGAAGGATATGTACCTATGGCATATGATGAGTATCTGAAAAAAATTAGCGAGCGTGGCGAATGGGGCGACCATGTTACTTTGCAGGCTGCTGCAGATTGTTATGGAGTTAAAATATGTGTGGTGACATCCTTCAAGGATACTTGCTTCATTGAAATTCTACCAAAAGTTCAACGTTCAAAACGAGTGATATTCTTGAGCTTTTGGGCAGAAGTGCACTATAATTCCATCTATCCTGAAGGGGAGCTACCAAATTTTTGTGAAAATAAGAGGAAGAAGCGTTGGTGGATCTTTGGACATAGACACTGAAGTTATAATGTATTCTTTCTTCATGGTATCATAAACTGAATCTCTTGCCTGTTTATGAGATTTTAGTGCTGATGTCTGTTTATCAACTATTATCTTGTACCTACTATTGTTATCAAGTCCTCTTAAGAGTGGCAGGTTTTTCTCTCATATACTCATTAGGAGGTCATATCGTCTTCCTTGGAGAAATAGAAAAGAAGAGACATTTGAAAGTATATCTGGCTAAGAGAAAGAGCAAATTCTGTGTCTTTTCTCATGGATTTTCAGTCAATCCATTGGAGACTAGGTATAGGAAGCATCTGTCTGAGTGATTGAAACCTCCTAATTGCTGCTTCATAATGTCAAAGCAGTGCCCCTACCATTAATCATGATGTAGTTGCCGGAAATGTTATAATGACTGCCACTAAGCAGTAATGGATCCTTATATCATAATCAATGAAGAAAATTGTTTATATTTTACATATGTGAGGACCATGACTTCAGAATG

Ace file of the UniGene sp_v3.0_unigene31189 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	OTU-like cysteine protease family protein [Arabidopsis thaliana] ref\|NP_001119168.1\| OTU-like cysteine protease family protein [Arabidopsis thaliana] gb\|AAM64524.1\| unknown [Arabidopsis thaliana] dbj\|BAD95211.1\| hypothetical protein [Arabidopsis thaliana]	-	-	0.0	46%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	59%
Sma3	Cysteine-type peptidase, putative	-	-	3.52e-15	-

Source	Gene names
Sma3	AT3G02070; At3g02070; At3g22260; At5g03330; At5g04250; F12E4_60; F1C9.14; F21E1_170; GSVIVT00018158001; GSVIVT00018159001; GSVIVT00023222001; GSVIVT00024580001; GSVIVT00033317001; GSVIVT00037995001; LOC_Os03g39230; LOC_Os03g64219; MICPUN_54840; OJ1202_E07

Source	GOs	Term	Type	Identity
Sma3	intracellular	GO:0005622	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	RNA binding	GO:0003723	Molecular Function	-
Sma3	RNA-directed DNA polymerase activity	GO:0003964	Molecular Function	-
Sma3	endonuclease activity	GO:0004519	Molecular Function	-
Sma3	peptidase activity	GO:0008233	Molecular Function	-
Sma3	RNA-dependent DNA replication	GO:0006278	Biological Process	-
Sma3	DNA repair	GO:0006281	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3		IPR000097	-	-
Sma3	Reverse transcriptase	IPR000477	-	-
Sma3	Ovarian tumour, otubain	IPR003323	-	-
Sma3	Endonuclease/exonuclease/phosphatase	IPR005135	-	-
Sma3		IPR015706	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G04250.1	Cysteine proteinases superfamily protein chr5:1176397-1178492 FORWARD LENGTH=345	48%
RefSeq	Arabidopsis thaliana	NP_568136.1	OTU-like cysteine protease family protein [Arabidopsis thaliana]	48%
RefSeq	Populus trichocarpa	XP_002331229.1	predicted protein [Populus trichocarpa]	52%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8J8

Fln msg: your sequence is longer than subject: 351 - 225

Fln protein: MVTYEPDPDVVRWGLHLLDGDSFSNTGHRINATQLSDTTLYRGIQIMEPQLPREPTNVENDEIIAHALQEELSHLAAVEASGDPHPTEDHLQASILAQDWLGPSQRISSEGAWHVEEQQESSHEQEVASPCSSLERRSQDGSEDVYTSLELLDEFSALDGEVGKRLTQMNSVPHIPRINGDIPTMDEATTDHQRLLDRLTLYDLRELKIPGDGNCQFRALSDQFYRTPEHHEFVRQQVVDQLKSEPELYEGYVPMAYDEYLKKISERGEWGDHVTLQAAADCYGVKICVVTSFKDTCFIEILPKVQRSKRVIFLSFWAEVHYNSIYPEGELPNFCENKRKKRWWIFGHRH*

Protein Length: 352

Fln nts: GTTTCAATTACAGGAAAACCATGATTCTGTTCATGCGTGCCCCGGGTCTTTATTTGTTTGTCGAAATAGCTGCATCCGAATCAGCAGGAGGAGGGGGGCATAGCACTGGCAGGGGAAGTAGCATAGCAAGCAACCAGCCAGGGAATTATATCAATTAAGGTGAGGAGAATTTCACACCTTCCATCAAAGGCAAGAGAGATCGATCTTTCCTTACCGATCTAATAACAAGGCAGGCACGAAACAACCTTCAAATTCTTCCCGACCTTTAACTCCTTCCTGATCTTCTAGCTCTTTTCCCCAGACATTGGAAGAGTTTTATGCCATGCTTTATCGATGCTTGTTATACTATAAAGGCCGTGTTTGTGTATTTCCAATGTTCTATACCCTTCGTTGCCTCTAAATTGTCGTCGTCTCCGAGGATTGAGTATAGAAGGAACTGTCAGAAGCCTCTTCCAGAATTTAGCGGATTCGGTTTGACTCATTCTCGCCATTTAATTTGAAGGTTTGTTGCTTTCGGATTTCGCATTCTTGAGGTTCTTTCTCAGCAAGTCCATGGCGGGAAAATTGGACCAGTGAAGCAGCCGTGATTGTATTACTGCATATGTGTCTTCTGTATTCATGTAGTCAACACGTATCTGATTTTGACTATCTTCTACCAGTGGACAACTTTGTTAGTATGGTGCACTTTTGAAATGTGTAGTACAACAGGTTATATACTTGAAAACTATATATTAGATCACATATGCCTTCTGTATTGGCAGACAGGTAGAAGTAGCAGTTAATTGATAGAGGGTACATTGATTTATCCTTGAGTTCAATATATAGGTCCTTATCTGAGCAACGACACTTCAAGGGAAACTAAGTTGTGTGAACAA_-_ATGGTGACATATGAGCCAGATCCAGATGTTGTCCGTTGGGGTCTCCATCTTCTTGATGGAGATTCTTTTTCCAATACAGGTCATCGGATTAATGCCACTCAATTGAGTGATACTACTCTATATCGGGGGATTCAGATTATGGAACCTCAATTGCCAAGAGAGCCCACTAATGTAGAAAATGATGAGATCATTGCTCATGCTCTTCAAGAAGAATTATCTCATCTGGCAGCTGTGGAAGCATCAGGTGACCCACACCCTACTGAAGATCATTTACAGGCCTCAATTCTTGCACAGGATTGGCTTGGTCCAAGCCAGCGAATATCTAGTGAAGGTGCATGGCATGTTGAGGAACAACAAGAATCAAGTCATGAACAAGAGGTTGCAAGTCCGTGCTCAAGTCTGGAAAGAAGGTCTCAAGATGGTTCTGAGGATGTCTATACATCTCTTGAGCTATTGGATGAGTTTTCTGCTTTGGATGGGGAAGTTGGCAAAAGATTGACGCAGATGAACTCTGTGCCTCATATTCCTAGGATTAATGGAGACATTCCTACCATGGATGAAGCAACCACAGACCATCAAAGGCTGTTGGACAGGTTAACACTCTATGATTTGCGTGAACTTAAAATTCCTGGAGATGGAAACTGCCAGTTTCGAGCACTCTCTGATCAGTTTTATCGAACACCTGAGCATCATGAGTTTGTCAGGCAACAGGTTGTTGACCAGCTAAAATCTGAACCAGAATTATATGAAGGATATGTACCTATGGCATATGATGAGTATCTGAAAAAAATTAGCGAGCGTGGCGAATGGGGCGACCATGTTACTTTGCAGGCTGCTGCAGATTGTTATGGAGTTAAAATATGTGTGGTGACATCCTTCAAGGATACTTGCTTCATTGAAATTCTACCAAAAGTTCAACGTTCAAAACGAGTGATATTCTTGAGCTTTTGGGCAGAAGTGCACTATAATTCCATCTATCCTGAAGGGGAGCTACCAAATTTTTGTGAAAATAAGAGGAAGAAGCGTTGGTGGATCTTTGGACATAGACACTGA___AGTTATAATGTATTCTTTCTTCATGGTATCATAAACTGAATCTCTTGCCTGTTTATGAGATTTTAGTGCTGATGTCTGTTTATCAACTATTATCTTGTACCTACTATTGTTATCAAGTCCTCTTAAGAGTGGCAGGTTTTTCTCTCATATACTCATTAGGAGGTCATATCGTCTTCCTTGGAGAAATAGAAAAGAAGAGACATTTGAAAGTATATCTGGCTAAGAGAAAGAGCAAATTCTGTGTCTTTTCTCATGGATTTTCAGTCAATCCATTGGAGACTAGGTATAGGAAGCATCTGTCTGAGTGATTGAAACCTCCTAATTGCTGCTTCATAATGTCAAAGCAGTGCCCCTACCATTAATCATGATGTAGTTGCCGGAAATGTTATAATGACTGCCACTAAGCAGTAATGGATCCTTATATCATAATCAATGAAGAAAATTGTTTATATTTTACATATGTGAGGACCATGACTTCAGAATG

Fln Alignment:

Contig31189___QDGSEDVYTSLELLDEFSALDGEVGKRLTQMNSVPHIPRINGDIPTMDEATTDHQXXXXXXXXXXXXXLKIPGDGNCQFRALSDQFYRTPEHHEFVRQQVVDQLKSEPELYEGYVPMAYDEYLKKISERGEWGDHVTLQAAADCYGVKICVVTSFKDTCFIEILPKVQRSKRVIFLSFWAEVHYNSIYPEGELPNFCENKRKKRWWIF
D5A8J8_______________QDIEDDRMIATILSEEYASLDRSVGRRLSHLQSIPHTPRINVYIPSANEATLDHQRLHDRLTIYGLCELKIQGDGNCQFRALSDQLYHSPEYHKYVRKEVVKQLKSFQSAYEGYVPMRYKDYLKKMKKSGEWGDHVTLQSAADRFGAKICLVTSFRDTCFIEIVPKQLNQRKELWLSFWSEVHYNSLYEIGEVP---IRVQKKKHWLF

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
605		80	20		0.995493
607		80	20		0.995493
609		80	20		0.995493
610	20		80		0.995493
611		20		80	0.995493
612	20			80	0.995493
613			20	80	0.995493
614		20		80	0.995493
615		20	80		0.995493
618		80	20		0.995493
621	20			80	0.995493
622		20	80		0.995493
623	80		20		0.995493
626			20	80	0.995493
755		20		60	0.99255
756		80		20	0.995493
2208	75		25		0.996473
2318	20	80			0.995493
2370	50	50			1.0
2373		25	75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain