SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene28614

Length: 3513 nt

UniGene Fasta

>sp_v3.0_unigene28614
CTCAAAAGTCTCTGATCCGTATTCTATTCCGATCCTGACGGACATGGACATCATCCATTCGCGGCGGTGGATTCTCCGCTAGTTTATACTCCAAATTCTCAGTAATTCATGCCATTGATACGCTGCAGATTCATAAAATGGCACGTCTGTATAGCTGTTTGGTGCTCCAAAGCCTGTAATTTAATTCTTATTTTGAAGAAGCAGATGGCCGCCATAAATAAATTCATCCAAGTCCCTGCTGCTTCCAGGGAAAGCTCGGTAGTGTCTGTGTGTAAAAATTCAAGCGGGGGAAGGAATGTGTCTGCAAATTTATTAGAATTTAGCAGGGACTTTAAACACTTTGTAAGAACTCCGGCCAATAAACTGAGGTCCTGTAGACATGGATACAGAAAAGGAAAATTATTATCGGGATACTCCGGCTTTGATTTTTACACGCCCTCCAAGAGAAGATTCAGAATCGTGGCTGCTGCTTTGACAGAGCAATTTACCCTGGGTAAAAGCGCTGATTCTCTACCGACATCTTTTGCCATAAATTTTCCCCTGACAAGAACAGAGAAGGTAGAGGGAAAGATCAGGGCAAAAGTAAGTCCTGCCAATAAAGACGGCAAATTGAAACTCACTTTGGAATGTGACCTGCCGGGAAGCTGGGTGCTGCACTGGGGAGTTACTTATTCCGATGATCCAGTCAGTGAATGGGATCAGCCACCTGCTGATATGCAACCTCCTGGCTCAGTCATAGTGAAGGATTATGCAGCACAAACACCATTAGTGCAGGAAGAGTCTCCAGAAGGTCAATGGTTGAGTTATGTGCAGATTAGCATCGACAATAGTAAGTCTGTAGCAGCTATTAATTTTCTATTAAAGGATGAGGACACAGCAACATGGTTCAAAGATGGTGACAAAGACTTCAGGGTTCTTCTTGCAGAAGCTATACCAAAAAGTTTTTATGACTCCAGCAATGGTGCAGGTTTAAAAGTGTGGCCAGGAATCCTTGATTGGATGTCAGGCATGTTCCTCAAGCCCAAGGAGTCCCCGCCACAAACATTACAAAATGGAAGTGAGATTGAAGATGCCAATACCAGCAATCAAATTGGTGACGGTGTTTGTAAAGAGTACAAGCTTTCAAAGGAAAATGTACTTAAGAATGTTCTACGAGTTTCTGTTCAGAAAAATACTGAAGAAAAACTAAATTTGGTGTCTTTTGACACTGACCTTCCCGGTGATGTTGTGCTTCATTGGGGAGTGTGTAAAGATGATTCTAAAAGATGGGAAATTCTTCGTCAGGGTGAATATCCCTCTGATACTATTGTATTTAGAAAGAGAGCCCTTCAAACTACGTTTCAGAGAAAAGACAATGGGAATGGAAGTTATGGTGTGCTTTGTATTGATGATCAGCATACGGGGTTGCTTTTTGTGCTCAAACTTAAGGGGGACAATACCTGGTTGAACAATAATGGGGATGATTTTTATATTCCTCTTCAAGGCTCGCTGAGTGTTTCATCTGCTGAAGCTCCAAATTCTCTAATGAATGAAACAAAAAAGACCTTGGATGGAATGCAGAGAATAAAATGAGGAAGGGAAGATGGAGAATGCCATATTAGATGAGGGTCAACAGGAGTTGGCCAGTGTTGAAAGCAATCCAAGTGAGCTGAAAACTCAGAGTTTTCCAGATAAACAGATTGGAAGTATTGAGTATAAAGATGCAGCAACTATTCTTCTTAACAAAGAAATAAAGAGCTTGGTCGAAAGAATTTCTGCTGAGTCTAGTGCGTCAGTGGGAAGTAATGAAACACAAGAAAAGTTTCTTCAAGAGATTGAAAGATTGGCTGCTAAGGCCTATAATAGCTTTAAAGATTCGACTGCTGTGTTTACAGAGGAAAGCAAGCCTGCAGTGCAGGCTTGGAAACCCAAGGGAAAGCCATGCTCAGGGACTGGATCAGGATACGAAGTTGTCCTTCAGGGTTTTAACTGGGAATCTCATCGCTCTGGAGGATGGTATCATAAGCTTTCTGAGAAAGCAGCTGAAATAGCCTCAAGGGGATTCACGATTGTTTGGCTTCCACCACCCACTGATTCTGTCTCTCCAGAAGGTTACATGCCACGTGACTTGTATAATCTGAATTCAAGATATGGAAGCATGGATGAATTGAAGGATACTGTCACAAGGTTCCATGAGGTTGGGGTAAAAGTCCTTGGAGATGCTGTCTTAAATCACCGCTGTGCTCAATACCAAAATCACAATGGGGTCTGGAATATTTTTGGTGGCCGTTTGAACTGGGATGATCGTGCAATTGTGTCTGATGATGCACATTTTCAGGGCAGGGGCAACAGAAGTAGTGGTGACTTTTTCCATGCAGCTCCAAATATTGACCATTCACAAGAGTTTGTGCGACGCGACTTGATTGATTGGCTGCAGTGGCTAAGGAGTGAAATTGGATATGATGGATGGAGATTAGATTTTGTGAGAGGATTTTGGGGAGGTTATGTGAAAGATTATATACAGGGAAGTGAACCATACTTTGCTGTCGGGGAGTATTGGGATTCACTTTCATACACATATGGTCAAATGGACCATGATCAAGATGCCCATCGGCAACGTATTATTGACTGGATAAATGCCACTGATGGAAATGCGGGAGCTTTTGATGTCACTACAAAGGGTATTCTTCATACGGTGCTGGAAAAATGTGAGTACTGGCGTTTATCTGATAAGAAAGGCAAACCTCCCGGGGTGGTTGGATGGTGGCCATCACGAGCAGTTACATTTATTGAGAATCATGATACTGGCTCAACTCAGGGTCACTGGAGATTTCCTGCTGGGAAAGAAATGCAAGGTTATGCATATATTTTGACGCATCCCGGAACTCCTGCTGTTTTCTATGATCATTTGTTTTCAAATAATTATAATGGGATTTCCTCTCTCATTGCTGTAAGAAAGCGTGCAGGAATCCATTGCCGCAGTGTGATCCAGATAGTGAAAGCAGAGAGAGATGTGTATGCAGCAAAGATTGATGAACGAATTTTTGTGAAGATTGGACCTGGGCACTATCAACCCCCAAATTCCTCTAAATGGATCGTGGCGACTGAGGGCCCAGACTACAAGGTTTGGGAGGCATTATGAGAATTTATTTGTTTTTATGCATGAGGTTCTTTGTCAAGAATGCAAATCGATTATTTTATTCAAAACTGATAAAAAAATGGCATGTATTATAGTTGATATATTATTACTGGTGACGACATGTCAAGCTTCCATTAGTTATCCTAGATGTATTGTGGCAAGGGCAAAGATACAAGGTTTTGGAGGCATTATGATAATCCTTAACGTATACATTTTACAATGAAAATACAAGCCATATGTTTCGCCCAAAATTTACATCTTCTGTAAAACAGGATGTATTATTGGGAGTGTAATATCATTAGGTTCTAGTTATACTCTTATGTATTCACTTCTACTTTTTGTGTTGTACAATTTCTATCTAAAATAAAAATCCTCAATTAGTAAATTTTTTCTCC

Ace file of the UniGene sp_v3.0_unigene28614

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	alpha-amylase-like 3 [Arabidopsis thaliana] gb\|AAK91414.1\| At1g69830/T17F3_14 [Arabidopsis thaliana] gb\|AAN18209.1\| At1g69830/T17F3_14 [Arabidopsis thaliana] gb\|AEE34983.1\| alpha-amylase-like 3 [Arabidopsis thaliana]	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	47%
Sma3	Alpha-amylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Alpha-amylase.	EC:3.2.1.1	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Starch and sucrose metabolism	00500		0.0	%
Sma3	Metabolic pathways	01100		0.0	%
Sma3		EC:3.2.1.6-	-	3.928e-17	-

Source	Gene names
Sma3	AAM1; ALPHA-AMY3; AMY1; AMY1.1; AMY1.2; AMY1.3; AMY1.4; AMY1.5; AMY1.6; AMY1.7; AMY10; AMY1A; AMY2; AMY2A; AMY3; AMY3A; AMY3B; AMY3D; AMY3E; AMY4; AMY5; AMY9; AMYA1; AMYA2; AMYC2; AT4g25000; Aamy1; Aamy2; Aamy3; Amy23; Amy32b; Amy46; AmyF1; At1g69830; At1

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	alpha-amylase activity	GO:0004556	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	starch catabolic process	GO:0005983	Biological Process	-
Sma3	sucrose catabolic process	GO:0005987	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Phosphotransferase system, HPr histidine phosphorylation site	IPR001020	-	-
Sma3	Phosphoribosylglycinamide formyltransferase, active site	IPR001555	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Glycoside hydrolase, family 13	IPR006046	-	-
Sma3	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	-
Sma3	Glycosyl hydrolase, family 13, subfamily, catalytic domain	IPR006589	-	-
Sma3	Alpha-amylase, C-terminal beta-sheet	IPR012850	-	-
Sma3	Alpha-amylase, plant	IPR013775	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G69830.1	ATAMY3, AMY3 alpha-amylase-like 3 chr1:26288518-26293003 REVERSE LENGTH=887	72%
RefSeq	Arabidopsis thaliana	NP_564977.1	alpha-amylase-like 3 [Arabidopsis thaliana]	72%
RefSeq	Populus trichocarpa	XP_002324108.1	predicted protein [Populus trichocarpa]	60%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUX3

Fln msg: your sequence is longer than subject: 513 - 448

Fln protein: MENAILDEGQQELASVESNPSELKTQSFPDKQIGSIEYKDAATILLNKEIKSLVERISAESSASVGSNETQEKFLQEIERLAAKAYNSFKDSTAVFTEESKPAVQAWKPKGKPCSGTGSGYEVVLQGFNWESHRSGGWYHKLSEKAAEIASRGFTIVWLPPPTDSVSPEGYMPRDLYNLNSRYGSMDELKDTVTRFHEVGVKVLGDAVLNHRCAQYQNHNGVWNIFGGRLNWDDRAIVSDDAHFQGRGNRSSGDFFHAAPNIDHSQEFVRRDLIDWLQWLRSEIGYDGWRLDFVRGFWGGYVKDYIQGSEPYFAVGEYWDSLSYTYGQMDHDQDAHRQRIIDWINATDGNAGAFDVTTKGILHTVLEKCEYWRLSDKKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPAGKEMQGYAYILTHPGTPAVFYDHLFSNNYNGISSLIAVRKRAGIHCRSVIQIVKAERDVYAAKIDERIFVKIGPGHYQPPNSSKWIVATEGPDYKVWEAL*

Protein Length: 514

Fln nts: CTCAAAAGTCTCTGATCCGTATTCTATTCCGATCCTGACGGACATGGACATCATCCATTCGCGGCGGTGGATTCTCCGCTAGTTTATACTCCAAATTCTCAGTAATTCATGCCATTGATACGCTGCAGATTCATAAAATGGCACGTCTGTATAGCTGTTTGGTGCTCCAAAGCCTGTAATTTAATTCTTATTTTGAAGAAGCAGATGGCCGCCATAAATAAATTCATCCAAGTCCCTGCTGCTTCCAGGGAAAGCTCGGTAGTGTCTGTGTGTAAAAATTCAAGCGGGGGAAGGAATGTGTCTGCAAATTTATTAGAATTTAGCAGGGACTTTAAACACTTTGTAAGAACTCCGGCCAATAAACTGAGGTCCTGTAGACATGGATACAGAAAAGGAAAATTATTATCGGGATACTCCGGCTTTGATTTTTACACGCCCTCCAAGAGAAGATTCAGAATCGTGGCTGCTGCTTTGACAGAGCAATTTACCCTGGGTAAAAGCGCTGATTCTCTACCGACATCTTTTGCCATAAATTTTCCCCTGACAAGAACAGAGAAGGTAGAGGGAAAGATCAGGGCAAAAGTAAGTCCTGCCAATAAAGACGGCAAATTGAAACTCACTTTGGAATGTGACCTGCCGGGAAGCTGGGTGCTGCACTGGGGAGTTACTTATTCCGATGATCCAGTCAGTGAATGGGATCAGCCACCTGCTGATATGCAACCTCCTGGCTCAGTCATAGTGAAGGATTATGCAGCACAAACACCATTAGTGCAGGAAGAGTCTCCAGAAGGTCAATGGTTGAGTTATGTGCAGATTAGCATCGACAATAGTAAGTCTGTAGCAGCTATTAATTTTCTATTAAAGGATGAGGACACAGCAACATGGTTCAAAGATGGTGACAAAGACTTCAGGGTTCTTCTTGCAGAAGCTATACCAAAAAGTTTTTATGACTCCAGCAATGGTGCAGGTTTAAAAGTGTGGCCAGGAATCCTTGATTGGATGTCAGGCATGTTCCTCAAGCCCAAGGAGTCCCCGCCACAAACATTACAAAATGGAAGTGAGATTGAAGATGCCAATACCAGCAATCAAATTGGTGACGGTGTTTGTAAAGAGTACAAGCTTTCAAAGGAAAATGTACTTAAGAATGTTCTACGAGTTTCTGTTCAGAAAAATACTGAAGAAAAACTAAATTTGGTGTCTTTTGACACTGACCTTCCCGGTGATGTTGTGCTTCATTGGGGAGTGTGTAAAGATGATTCTAAAAGATGGGAAATTCTTCGTCAGGGTGAATATCCCTCTGATACTATTGTATTTAGAAAGAGAGCCCTTCAAACTACGTTTCAGAGAAAAGACAATGGGAATGGAAGTTATGGTGTGCTTTGTATTGATGATCAGCATACGGGGTTGCTTTTTGTGCTCAAACTTAAGGGGGACAATACCTGGTTGAACAATAATGGGGATGATTTTTATATTCCTCTTCAAGGCTCGCTGAGTGTTTCATCTGCTGAAGCTCCAAATTCTCTAATGAATGAAACAAAAAAGACCTTGGATGGAATGCAGAGAATAAAATGAGGAAGGGAAG_-_ATGGAGAATGCCATATTAGATGAGGGTCAACAGGAGTTGGCCAGTGTTGAAAGCAATCCAAGTGAGCTGAAAACTCAGAGTTTTCCAGATAAACAGATTGGAAGTATTGAGTATAAAGATGCAGCAACTATTCTTCTTAACAAAGAAATAAAGAGCTTGGTCGAAAGAATTTCTGCTGAGTCTAGTGCGTCAGTGGGAAGTAATGAAACACAAGAAAAGTTTCTTCAAGAGATTGAAAGATTGGCTGCTAAGGCCTATAATAGCTTTAAAGATTCGACTGCTGTGTTTACAGAGGAAAGCAAGCCTGCAGTGCAGGCTTGGAAACCCAAGGGAAAGCCATGCTCAGGGACTGGATCAGGATACGAAGTTGTCCTTCAGGGTTTTAACTGGGAATCTCATCGCTCTGGAGGATGGTATCATAAGCTTTCTGAGAAAGCAGCTGAAATAGCCTCAAGGGGATTCACGATTGTTTGGCTTCCACCACCCACTGATTCTGTCTCTCCAGAAGGTTACATGCCACGTGACTTGTATAATCTGAATTCAAGATATGGAAGCATGGATGAATTGAAGGATACTGTCACAAGGTTCCATGAGGTTGGGGTAAAAGTCCTTGGAGATGCTGTCTTAAATCACCGCTGTGCTCAATACCAAAATCACAATGGGGTCTGGAATATTTTTGGTGGCCGTTTGAACTGGGATGATCGTGCAATTGTGTCTGATGATGCACATTTTCAGGGCAGGGGCAACAGAAGTAGTGGTGACTTTTTCCATGCAGCTCCAAATATTGACCATTCACAAGAGTTTGTGCGACGCGACTTGATTGATTGGCTGCAGTGGCTAAGGAGTGAAATTGGATATGATGGATGGAGATTAGATTTTGTGAGAGGATTTTGGGGAGGTTATGTGAAAGATTATATACAGGGAAGTGAACCATACTTTGCTGTCGGGGAGTATTGGGATTCACTTTCATACACATATGGTCAAATGGACCATGATCAAGATGCCCATCGGCAACGTATTATTGACTGGATAAATGCCACTGATGGAAATGCGGGAGCTTTTGATGTCACTACAAAGGGTATTCTTCATACGGTGCTGGAAAAATGTGAGTACTGGCGTTTATCTGATAAGAAAGGCAAACCTCCCGGGGTGGTTGGATGGTGGCCATCACGAGCAGTTACATTTATTGAGAATCATGATACTGGCTCAACTCAGGGTCACTGGAGATTTCCTGCTGGGAAAGAAATGCAAGGTTATGCATATATTTTGACGCATCCCGGAACTCCTGCTGTTTTCTATGATCATTTGTTTTCAAATAATTATAATGGGATTTCCTCTCTCATTGCTGTAAGAAAGCGTGCAGGAATCCATTGCCGCAGTGTGATCCAGATAGTGAAAGCAGAGAGAGATGTGTATGCAGCAAAGATTGATGAACGAATTTTTGTGAAGATTGGACCTGGGCACTATCAACCCCCAAATTCCTCTAAATGGATCGTGGCGACTGAGGGCCCAGACTACAAGGTTTGGGAGGCATTATGA___GAATTTATTTGTTTTTATGCATGAGGTTCTTTGTCAAGAATGCAAATCGATTATTTTATTCAAAACTGATAAAAAAATGGCATGTATTATAGTTGATATATTATTACTGGTGACGACATGTCAAGCTTCCATTAGTTATCCTAGATGTATTGTGGCAAGGGCAAAGATACAAGGTTTTGGAGGCATTATGATAATCCTTAACGTATACATTTTACAATGAAAATACAAGCCATATGTTTCGCCCAAAATTTACATCTTCTGTAAAACAGGATGTATTATTGGGAGTGTAATATCATTAGGTTCTAGTTATACTCTTATGTATTCACTTCTACTTTTTGTGTTGTACAATTTCTATCTAAAATAAAAATCCTCAATTAGTAAATTTTTTCTCC

Fln Alignment:

Contig28614___VVLQGFNWESHR-SGGWYHKLSEKAAEIASRGFTIVWLPPPTDSVSPEGYMPRDLYNLNS-RYGSMDELKDTVTRFHEVGVKVLGDAVLNHRCAQYQNHNGVWNIF-----GGRLNWDDRAIVSDDAHFQ-GRGNRSSGDFFHAAPNIDHSQEFVRRDLIDWLQWLRSEIGYDGWRLDFVRGFWGGYVKDYIQGSEPYFAVGEYWDSLSYTYGQMDHDQDAHRQRIIDWINATDGNAGAFDVTTKGILHTVLEKCEYWRLSDKKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPAGKEMQGYAYILTHPGTPAVFYDHLFSNNY-NGISSLIAVRKRAGIHCRSVIQIVKAERDVYAAKIDERIFVKIGP----GHYQPPNSSKWIVATEGPDYKVWE
A9NUX3_______________LLLQGFNWESAKGSSSWYNVLKGIVEDAADAGITDVWFPPPSQSLAPEGYLPQRLYDLNSSKYGSEQELRNAVDAFHQKGIGCVGDMVINHRSGTKQDDKGMWCVFEGGKGDGRLDWGPWAVTVNDKPFACGSGQADTGGDYAAAPDIDHTNPKIQQDLSEWMNWLKSNVGFDGWRFDFAKGYAGNLLAVYLKNTSPKFAVGEVWDDMSYSDGRLAYDQGAHRQRLVDWVHSTGDGAAAFDFTTKGILQEAVKSNELWRLKDSNGKPAGMIGVLPQKAVTFIDNHDTGSTQNIWPFPSDKLLQGYAYILTHPGIPTIFYDHFVDGNLKKEIQNLSAIRKRNNINANSNCRIITAESDLYMAAIDEKIVVKIGSRYDVGNLAPP-SPEFRVVASGNGYCVWE

SNPs (tot: 30)

Position	A %	C %	G %	T %	Probability
439	14			85	0.993533
443	85	14			0.993533
446		14	85		0.993533
447	85			14	0.993533
781	25			75	0.996473
784		75		25	0.996473
932		12		87	0.992554
938	83		16		0.994512
939	83			16	0.994512
940	83	16			0.994512
943		16		83	0.994512
944			16	83	0.994512
945			16	83	0.994512
1167			90	10	0.90298
1168	90			10	0.90298
1362	50			50	1.0
2490			83	16	0.994512
3007		22		77	0.999999
3009		77		22	0.999999
3010	77	22			0.999999
3012		77		22	0.999999
3013	77		22		0.999999
3015	77	22			0.999999
3016		22	77		0.999999
3017	77			22	0.999999
3019			22	77	0.999999
3021	77	22			0.999999
3024	77	22			0.999999
3027	11	11	77		0.968132
3376	33	66			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain