SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene28339

Length: 958 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene28339 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Whole genome shotgun sequence of line PN40024, scaffold_89.assembly12x (Fragment) n=1 Tax=Vitis vinifera RepID=D7SMT7_VITVI	-	-	0.0	48%
FL-Next	sp=Reticuline oxidase-like protein; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	39%
Sma3	Reticuline oxidase, putative	-	-	3.063e-30	-

Source	ECs	Term	Type	e value	Identity
Sma3	Reticuline oxidase.	EC:1.21.3.3	-	1.882e-32	-

Source	KEGGs	Term	e value	Identity
Sma3	Isoquinoline alkaloid biosynthesis	00950	1.882e-32	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063	1.882e-32	%
Sma3	Metabolic pathways	01100	1.882e-32	%
Sma3	Biosynthesis of secondary metabolites	01110	1.882e-32	%

Source	Gene names
Sma3	AT4g20820; AT4g20860; At1g26380; At1g26390; At1g26410; At1g26420; At1g30700; At1g30710; At1g30720; At1g30720/T5I8_17; At1g30730; At1g30760; At2g34790; At4g20820; At4g20860; At5g44360; At5g44380; At5g44390; At5g44390/K9L2.19; At5g44400; At5g44410; CBDAS; C

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	nucleic acid binding	GO:0003676	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	oxidoreductase activity	GO:0016491	Molecular Function	-
Sma3	transcription regulator activity	GO:0030528	Molecular Function	-
Sma3	flavin adenine dinucleotide binding	GO:0050660	Molecular Function	-
Sma3	response to oxidative stress	GO:0006979	Biological Process	-
Sma3	embryo development ending in seed dormancy	GO:0009793	Biological Process	-
Sma3	polar nucleus fusion	GO:0010197	Biological Process	-
Sma3		GO:0045449	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Legume lectin domain	IPR001220	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	Enolpyruvate transferase domain	IPR001986	-	-
Sma3	Retrotransposon gag protein	IPR005162	-	-
Sma3	Oxygen oxidoreductase covalent FAD-binding site	IPR006093	-	-
Sma3	FAD linked oxidase, N-terminal	IPR006094	-	-
Sma3	Helix-loop-helix DNA-binding	IPR011598	-	-
Sma3	Berberine/berberine-like	IPR012951	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-
Sma3	FAD-binding, type 2	IPR016166	-	-
Sma3	FAD-linked oxidase, FAD-binding, subdomain 2	IPR016168	-	-

Source	Species	ID	Description	e value	Identity
RefSeq	Populus trichocarpa	XP_002317088.1	predicted protein [Populus trichocarpa]	0.0	49%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: sp_plants

Fln subject: Q9SVG4

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 32 aas, putative chimeric sequence, your sequence is shorter than subject: 242 - 570

Fln protein: MRVQFFGIKVDGGTKRDMNASFQGMYLGRKEQFLKIVNITFPELKMAENECSEMSWIESTSFIGGTANVSELTNRYYSNKVHFKIKSDMATTPLSKPGLQGLWDIMEEEVSAYAIFSPLGGIMDRIPSSAIPFPHRAGNIFDIQYKVVWNNSSDDEHYLNWMRKLYAYMAPYVSSSPRSAYVNYLDLDLGSAINGTATVKEAEAWGKKYFHDNFDRLVKAKTEVDPHNFFRNSQSIPPRNN*

Protein Length: 243

Fln nts: TCATGCGTGTGCAGTTCTTCGGCATCAAAGTCGACGGAGGGACGAAAAGGGATATGAACGCGTCGTTCCAAGGGATGTATCTGGGACGTAAGGAGCAGTTCTTGAAGATTGTGAACATCACCTTCCCGGAGTTGAAGATGGCGGAGAACGAGTGCAGTGAAATGAGCTGGATCGAGTCTACGTCCTTCATCGGAGGGACTGCTAACGTGAGCGAACTGACAAACAGATACTATTCCAACAAAGTCCATTTCAAAATAAAATCAGATATGGCAACAACCCCGCTGTCCAAACCAGGTCTCCAAGGATTGTGGGACATCATGGAAGAAGAGGTTAGCGCTTATGCAATTTTCTCTCCTTTGGGAGGAATTATGGACAGGATTCCTTCCTCTGCTATACCCTTCCCTCACAGAGCTGGAAACATATTCGACATACAGTACAAGGTCGTATGGAACAACAGCAGTGACGATGAGCACTACCTTAACTGGATGCGAAAGCTTTACGCATACATGGCTCCTTATGTTTCTTCTTCTCCCAGATCTGCTTATGTTAATTATTTGGATCTGGATCTGGGCAGCGCAATCAATGGAACTGCCACAGTCAAAGAAGCCGAAGCATGGGGTAAGAAGTATTTCCACGACAATTTCGATAGACTTGTGAAGGCCAAAACAGAGGTCGATCCCCACAACTTTTTCAGAAATTCTCAGAGCATTCCTCCACGAAATAACTGA___TTATCCCTAATCTCTAATCATTTGGTTGATTGTAATCCAAAATAAGGCTACAGCTTAAGCCACTTCAGTCCTTAACGGCGTGGCACTCCTTGGAACGCCGAACATCAATTGTGGTGAATTCACGTCGAATTTGTATGTTTTTCTGGATGAATTTGCTTCTTTACGCTTTGCAATAAAAATACAGCACGACTGTTATATTTATCACCATGTAGAAATACAAGAGTGCACGG

Fln Alignment:

Contig28339___RDMNASFQGMYLGRKEQFLKIVNITFPELKMAENECSEMSWIESTSFIGGTANVSELTNRYYSNK-----VHFKIKSDMATTPLSKPGLQGLWDIMEEEVSAYAIFSPLGGIMDRIPSSAIPFPHRAGNIFDIQYKVVW--NNSSDDEHYLNWMRKLYAYMAPYVSSSPRSAYVNYLDLDLGSAINGTATVKEAEAWGKKYFHDNFDRLVKAKTEVDPHNFFRNSQSIPPRNN
Q9SVG4_______________KTVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLKN

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
219			70	30	1.0
225	10		90		0.90298
242	10		90		0.90298
267	12		87		0.992554
370		42	57		1.0
516	38		61		1.0
621	60		40		1.0
666		16		83	0.994512
758		33	66		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain