SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene23400

Length: 2209 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene23400
TAAACACAAGGGAGCATGCCCAGTGGCGTTTGGACCAGGGTCATTATTACATCAAGTGTGCCTTACCTCTAGACCATGGTCTGCTGGACTGGGAGAGATATTGTTGAAGTACTTGATGATTTGATCTGAAGTGTGCTTTTGTATGTTGGAAAGCATTCTAGGAGTAGCCATGGCCATTGTAGAATGTCAACGCTACCAGTATTAAGGTATTTCTCTTTTGTTTTGTAGGAAGTGTCGGAATTTCAGAAACCAAAAAGTGTGTGCTTCCATATTGAAAAACTCTTCCTTCTCCATTTTATCTCGGTTTTACAACATGGCAACAATGGCAAGCAATTTCAGTTCCAGTGTGCCAGATGGGTTTTCTGATACACCTCCAGAACCCAGAAGAACCTTCCAGATTGTAGTAGCTGCCACTAAGGAAATGGGAATTGGCAAGGATGGAAAGCTTCCATGGAAACTACCTTCTGATATGAAATTCTTCAAGGAAATCACATCTTCCACGTCAGCTCCTTTAAAGAAAAATGCTGTGATAATGGGACGAAAGACTTGGGAAAGTATTCCAGACCAGTTTCGACCACTTACAGGTCGCTTGAATGTTGTGCTAAGTCGTTCAGGTAGCTTTGACATTGCAACTGCAGAGAATGTTGTTATGTGTGGAAGTTTGGGTTCAGCTCTTGAGATGCTTGCAGCACCTCCATACTCTATGTCAATAGAGACAGTATTTGTAATTGGAGGTGGTCAAATTTATAGAGAAGCACTCTCTACACCTAACTGTGATGCAATTCACATCACTGAGATAGAGAATGCATTTGAGTGTGATACATTCATCACACCCATAGATGCATCTGTGTTTCGTCCTTGGTATTCGTCATTGCCATTTGTGGAAAATGATATTCGCTTTTCTTTCACCACCTATGTTCGTATTAGGAATTCCTCTAATGTGGACTCTCTAGAGCCTTCTCACAGATTTGGAAATGGATCGAACAACATGGATATGTACGATAGACTTGAATCTCGGCAGTATCAATTTCTCCCCAAATTAATTTTTGAAAGACACGAGGAGTATTTGTATCTTAGATTGGTACAGGATATAATTGCAAATGGAGTGGAGAAGGGAGATCGGACAGGAACTGGCACATTGTCAAAATTTGGCTGTCAGATGCGATTCAATCTGCGAAAAACTTTACCACTTCTCACAACAAAGCGAGTTTTCTGGCGTGGTGTGGTTGAAGAGCTCTTATGGTTCATAAGTGGTTCAACAAATGCAAAGGTTCTGCAAGAAAAGGGCATACATATATGGGATGGTAATGCATCGAGAGAGTACCTTGATAGCATTGGGCTGAAAGAAAGAGAGGAAGGTGATTTGGGACCTGTATATGGATTTCAGTGGAGGCATTTTGGAGCTAGGTATACAAACATGAATGCAGATTACAGTGGACAAGGTTTTGATCAACTAGCAGATGTAATTCATAAGATTAAGTTTAATCCAGAAGATCGTCGCATTATACTCTCAGCATGGAACCCAGCGGACCTTAAGCTCATGGCTCTTCCTCCATGCCACATGTTTGCTCAGTTTTATGTTGCAAAAGGGGAATTGTCATGCCAGATGTACCAACGATCCTGTGACATGGGCCTTGGCGTACCTTTCAACATTGCATCATATGCACTTTTAACATGCATGATTGCCCATGTTTGTGACCTTGTTCCCGGTGATCTTATCCATGTGCTTGGAGATGCTCACGTGTACAGAAACCATGTTGGGCCTCTTTTAGAACAACTGCAGAACTCACCTAGACCGTTTCCAGTTTTGAAAATCAATCCTGAAAAGAAAGATATCGATTCATTTGTTGCGAATGATTTCGAACTCATTGGATATGATTCTCATAAGAAAATAGAAATGAAAATGGCTGTTTAGTTTCTAGTTTGTAGAATGGCTTTTTTTGGCCTGGTAAAAATAGGTAATTTTCTGTTCTAGAAATCAATCCACATCAAGAATGATGTAATTTTCAGTCGTGGAAGTGAGCCATGAATCTTTCTTTTTAATATAGATGACGTACAGTAGAGAGAGGGGGATGCAATGATGTTATTTTGAGATGCATCACCATTTCAAAGGTTGATCGATCTGATGTTTCGAGGGGGTAACGCTTTTCCTTCTCGAGGAGTGTTACAATTTTGAAGAAGTCCGAAGCAATAAAAAATAGTATTTTCG

Ace file of the UniGene sp_v3.0_unigene23400 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	PREDICTED: similar to Bifunctional dihydrofolate reductase-thymidylate synthase n=1 Tax=Vitis vinifera RepID=UPI0001982DBD	-	-	0.0	73%
FL-Next	sp=Bifunctional dihydrofolate reductase-thymidylate synthase; Glycine max (Soybean) (Glycine hispida).	-	-	0.0	74%
Sma3	Thymidylate synthase	-	-	7.791e-33	-

Source	ECs	Term	Type	e value	Identity
Sma3	Dihydrofolate reductase.	EC:1.5.1.3	-	3.33495e-41	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	One carbon pool by folate	00670		3.33495e-41	%
Sma3	Folate biosynthesis	00790		3.33495e-41	%
Sma3	Biosynthesis of phenylpropanoids	01061		3.33495e-41	%
Sma3	Metabolic pathways	01100		3.33495e-41	%
Sma3	Thymidylate synthase.	EC:2.1.1.45	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Pyrimidine metabolism	00240	%
Sma3	One carbon pool by folate	00670	%
Sma3	Metabolic pathways	01100	%

Source	Gene names
Sma3	At2g16370; At2g21550; At4g34570; CHLREDRAFT_139742; CM0216.390.nc; DHFR-TS; DHFR-TS-1; DHFR-TS-2; DHFR-TS-3a; DHFR-TS-3b; DRTS; F16F14.13; GSVIVT00014567001; GSVIVT00014568001; GSVIVT00036343001; GSVIVT00036344001; LOC_Os11g29390; MICPUCDRAFT_56456; MICPU

Source	GOs	Term	Type	Identity
Sma3	phosphopyruvate hydratase complex	GO:0000015	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	dihydrofolate reductase activity	GO:0004146	Molecular Function	-
Sma3	phosphopyruvate hydratase activity	GO:0004634	Molecular Function	-
Sma3	thymidylate synthase activity	GO:0004799	Molecular Function	-
Sma3	binding	GO:0005488	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	NADP binding	GO:0050661	Molecular Function	-
Sma3	glycolysis	GO:0006096	Biological Process	-
Sma3	dTMP biosynthetic process	GO:0006231	Biological Process	-
Sma3	glycine biosynthetic process	GO:0006545	Biological Process	-
Sma3	one-carbon metabolic process	GO:0006730	Biological Process	-
Sma3	nucleotide biosynthetic process	GO:0009165	Biological Process	-
Sma3	DNA integration	GO:0015074	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Thymidylate synthase	IPR000398	-	-
Sma3	Enolase	IPR000941	-	-
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Dihydrofolate reductase domain	IPR001796	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	Dihydrofolate reductase	IPR012259	-	-
Sma3	Bifunctional dihydrofolate reductase/thymidylate synthase	IPR012262	-	-
Sma3		IPR013084	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Dihydrofolate reductase conserved site	IPR017925	-	-
Sma3	Peptidase S26A, signal peptidase I, serine active site	IPR019756	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G16370.1	THY-1 thymidylate synthase 1 chr2:7082038-7084333 REVERSE LENGTH=519	74%
RefSeq	Arabidopsis thaliana	NP_179230.1	thymidylate synthase [Arabidopsis thaliana]	74%
RefSeq	Populus trichocarpa	XP_002306118.1	dihydrofolate reductase-thymidylate synthase [Populus trichocarpa]	75%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: P51820

Fln msg:

Fln protein: MATMASNFSSSVPDGFSDTPPEPRRTFQIVVAATKEMGIGKDGKLPWKLPSDMKFFKEITSSTSAPLKKNAVIMGRKTWESIPDQFRPLTGRLNVVLSRSGSFDIATAENVVMCGSLGSALEMLAAPPYSMSIETVFVIGGGQIYREALSTPNCDAIHITEIENAFECDTFITPIDASVFRPWYSSLPFVENDIRFSFTTYVRIRNSSNVDSLEPSHRFGNGSNNMDMYDRLESRQYQFLPKLIFERHEEYLYLRLVQDIIANGVEKGDRTGTGTLSKFGCQMRFNLRKTLPLLTTKRVFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDSIGLKEREEGDLGPVYGFQWRHFGARYTNMNADYSGQGFDQLADVIHKIKFNPEDRRIILSAWNPADLKLMALPPCHMFAQFYVAKGELSCQMYQRSCDMGLGVPFNIASYALLTCMIAHVCDLVPGDLIHVLGDAHVYRNHVGPLLEQLQNSPRPFPVLKINPEKKDIDSFVANDFELIGYDSHKKIEMKMAV*

Protein Length: 535

Fln nts: TAAACACAAGGGAGCATGCCCAGTGGCGTTTGGACCAGGGTCATTATTACATCAAGTGTGCCTTACCTCTAGACCATGGTCTGCTGGACTGGGAGAGATATTGTTGAAGTACTTGATGATTTGATCTGAAGTGTGCTTTTGTATGTTGGAAAGCATTCTAGGAGTAGCCATGGCCATTGTAGAATGTCAACGCTACCAGTATTAAGGTATTTCTCTTTTGTTTTGTAGGAAGTGTCGGAATTTCAGAAACCAAAAAGTGTGTGCTTCCATATTGAAAAACTCTTCCTTCTCCATTTTATCTCGGTTTTACAAC_-_ATGGCAACAATGGCAAGCAATTTCAGTTCCAGTGTGCCAGATGGGTTTTCTGATACACCTCCAGAACCCAGAAGAACCTTCCAGATTGTAGTAGCTGCCACTAAGGAAATGGGAATTGGCAAGGATGGAAAGCTTCCATGGAAACTACCTTCTGATATGAAATTCTTCAAGGAAATCACATCTTCCACGTCAGCTCCTTTAAAGAAAAATGCTGTGATAATGGGACGAAAGACTTGGGAAAGTATTCCAGACCAGTTTCGACCACTTACAGGTCGCTTGAATGTTGTGCTAAGTCGTTCAGGTAGCTTTGACATTGCAACTGCAGAGAATGTTGTTATGTGTGGAAGTTTGGGTTCAGCTCTTGAGATGCTTGCAGCACCTCCATACTCTATGTCAATAGAGACAGTATTTGTAATTGGAGGTGGTCAAATTTATAGAGAAGCACTCTCTACACCTAACTGTGATGCAATTCACATCACTGAGATAGAGAATGCATTTGAGTGTGATACATTCATCACACCCATAGATGCATCTGTGTTTCGTCCTTGGTATTCGTCATTGCCATTTGTGGAAAATGATATTCGCTTTTCTTTCACCACCTATGTTCGTATTAGGAATTCCTCTAATGTGGACTCTCTAGAGCCTTCTCACAGATTTGGAAATGGATCGAACAACATGGATATGTACGATAGACTTGAATCTCGGCAGTATCAATTTCTCCCCAAATTAATTTTTGAAAGACACGAGGAGTATTTGTATCTTAGATTGGTACAGGATATAATTGCAAATGGAGTGGAGAAGGGAGATCGGACAGGAACTGGCACATTGTCAAAATTTGGCTGTCAGATGCGATTCAATCTGCGAAAAACTTTACCACTTCTCACAACAAAGCGAGTTTTCTGGCGTGGTGTGGTTGAAGAGCTCTTATGGTTCATAAGTGGTTCAACAAATGCAAAGGTTCTGCAAGAAAAGGGCATACATATATGGGATGGTAATGCATCGAGAGAGTACCTTGATAGCATTGGGCTGAAAGAAAGAGAGGAAGGTGATTTGGGACCTGTATATGGATTTCAGTGGAGGCATTTTGGAGCTAGGTATACAAACATGAATGCAGATTACAGTGGACAAGGTTTTGATCAACTAGCAGATGTAATTCATAAGATTAAGTTTAATCCAGAAGATCGTCGCATTATACTCTCAGCATGGAACCCAGCGGACCTTAAGCTCATGGCTCTTCCTCCATGCCACATGTTTGCTCAGTTTTATGTTGCAAAAGGGGAATTGTCATGCCAGATGTACCAACGATCCTGTGACATGGGCCTTGGCGTACCTTTCAACATTGCATCATATGCACTTTTAACATGCATGATTGCCCATGTTTGTGACCTTGTTCCCGGTGATCTTATCCATGTGCTTGGAGATGCTCACGTGTACAGAAACCATGTTGGGCCTCTTTTAGAACAACTGCAGAACTCACCTAGACCGTTTCCAGTTTTGAAAATCAATCCTGAAAAGAAAGATATCGATTCATTTGTTGCGAATGATTTCGAACTCATTGGATATGATTCTCATAAGAAAATAGAAATGAAAATGGCTGTTTAG___TTTCTAGTTTGTAGAATGGCTTTTTTTGGCCTGGTAAAAATAGGTAATTTTCTGTTCTAGAAATCAATCCACATCAAGAATGATGTAATTTTCAGTCGTGGAAGTGAGCCATGAATCTTTCTTTTTAATATAGATGACGTACAGTAGAGAGAGGGGGATGCAATGATGTTATTTTGAGATGCATCACCATTTCAAAGGTTGATCGATCTGATGTTTCGAGGGGGTAACGCTTTTCCTTCTCGAGGAGTGTTACAATTTTGAAGAAGTCCGAAGCAATAAAAAATAGTATTTTCG

Fln Alignment:

Contig23400___MATMASNFSSSVPDGFSDTPPEPRRTFQIVVAATKEMGIGKDGKLPWKLPSDMKFFKEITSSTSAPLKKNAVIMGRKTWESIPDQFRPLTGRLNVVLSRSGSFDIATAENVVMCGSLGSALEMLAAPPYSMSIETVFVIGGGQIYREALSTPNCDAIHITEIENAFECDTFITPIDASVFRPWYSSLPFVENDIRFSFTTYVRIRNSS------NVDSLEPSHRFGNGSNNMDMYDRLESRQYQFLPKLIFERHEEYLYLRLVQDIIANGVEKGDRTGTGTLSKFGCQMRFNXXXXXXXXXXXRVFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDSIGLKEREEGDLGPVYGFQWRHFGARYTNMNADYSGQGFDQLADVIHKIKFNPEDRRIILSAWNPADLKLMALPPCHMFAQFYVAKGELSCQMYQRSCDMGLGVPFNIASYALLTCMIAHVCDLVPGDLIHVLGDAHVYRNHVGPLLEQLQNSPRPFPVLKINPEKKDIDSFVANDFELIGYDSHKKIEMKMAV
P51820_______________MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEITMKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSALELLAASPYSLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQSSIECDTFMPPVDFTIFRPWYSSFPKVENNIRYSFTTYVRVRSSAAESAGQNIDPL-----LDNNSESM----KFEVKDFSFLPKMISERHEEYLYLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMRFNLRGNFPLLTTKKVFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDGVGLTEREEGDLGPVYGFQWRHFGARYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAWNPVDLKLMALPPCHMFAQFYVAHGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHVCDLIPGDFIHVIGDAHIYRNHVRPLQEQLHNQPKPFPTLKINPKKKDIDSFVAADFKLIGYDPHQKIDMKLSV

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
82	83		16		0.994512
1540		80		20	0.995493
1667		80		20	0.995493
1682		80	20		0.995493
1830	20		80		0.995493
1863	87	12			0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain