SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene22899

Length: 1478 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene22899

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Alpha-galactosidase n=2 Tax=Nicotiana tabacum RepID=F5BFS9_TOBAC	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Sma3	Alpha-galactosidase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Alpha-galactosidase.	EC:3.2.1.22	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Galactose metabolism	00052	%
Sma3	Glycerolipid metabolism	00561	%
Sma3	Sphingolipid metabolism	00600	%
Sma3	Glycosphingolipid biosynthesis - globo series	00603	%

Source	Gene names
Sma3	AGA1; At3g56310; At5g08370; At5g08380; CHLREDRAFT_116873; F18O21_270; F8L15_100; F8L15_110; GGT-1; GLA; GSVIVT00002617001; GSVIVT00002618001; GSVIVT00002619001; GSVIVT00011770001; GSVIVT00029420001; LOC_Os10g35070; LOC_Os10g35110; LeaGal; OJ1205_F02.7; OJ

Source	GOs	Term	Type	Identity
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	alpha-galactosidase activity	GO:0004557	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	transferase activity, transferring glycosyl groups	GO:0016757	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	positive regulation of flower development	GO:0009911	Biological Process	-
Sma3	leaf morphogenesis	GO:0009965	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, clan GH-D	IPR000111	-	-
Sma3	WW/Rsp5/WWP	IPR001202	-	-
Sma3	Glycoside hydrolase, family 27	IPR002241	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G08370.1	AtAGAL2, AGAL2 alpha-galactosidase 2 chr5:2691116-2693441 REVERSE LENGTH=396	71%
RefSeq	Arabidopsis thaliana	NP_001031855.1	alpha-galactosidase 2 [Arabidopsis thaliana]	71%
RefSeq	Populus trichocarpa	XP_002331416.1	predicted protein [Populus trichocarpa]	72%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ADT9

Fln msg: Overlapping hits, possible frame ERROR between 229 and 228,

Fln protein: MVSVSLVVIVFIALWDKTSEGRPIHRHLSSNGLGKTPQMGWNSWNHFGCNIDENIIRETADAxxVSSGLSKIGYEYVNIDDCWGELNRDNQGNLVAKASTFPSGIKALADYVHSKGLKFGIYSDAGNYTCGKTMPGSLGYEEQDAKTFASWGVDYLKYDNCFNDDINPKERYPKMSEALTNSGRPIFYSMCEWGDENPATWAPKIANSWRTTGDIQDNWDSITSRADMNDKWAAYAGPGGWNDPDMLEVGNGNMSNVEYRSHFSIWALMKAPLLIGCDIRSATSETLEILSNSEVINVNQDPLGVQGKKVSQEGDLEVWAGQVSNDRVAVILWNRSGSQAVITAKWEDIGLPSDAIVQARDLWTQSYLPSNLQSNLTSMVDSHGVKMYILTPSSG*

Protein Length: 397

Fln nts: ATCTTTATTGCAGTTCTCTAGCTTTATCCTTCCAGGAAAG_-_ATGGTATCTGTTTCTCTTGTGGTTATTGTTTTCATTGCATTGTGGGATAAGACCAGTGAAGGTCGACCAATTCATAGGCATTTATCCTCCAATGGGCTTGGCAAGACGCCTCAGATGGGCTGGAATAGTTGGAATCATTTCGGCTGCAACATTGATGAAAATATTATACGTGAAACAGCTGATGCAxxxxxxGTTTCTTCTGGTTTATCCAAGATTGGATATGAATACGTGAATATAGATGATTGTTGGGGTGAGCTAAACAGAGATAATCAGGGTAATCTGGTTGCTAAGGCTTCAACGTTTCCTTCCGGAATTAAGGCTCTCGCAGACTATGTACACTCTAAAGGACTCAAGTTTGGAATATATTCTGATGCTGGAAATTATACATGCGGCAAAACCATGCCAGGTTCCCTTGGATACGAGGAACAAGATGCTAAAACCTTTGCTTCATGGGGAGTAGACTACTTGAAATATGATAATTGTTTTAATGATGACATAAACCCTAAAGAGAGGTACCCAAAAATGAGCGAAGCTCTTACTAACTCAGGGCGTCCTATATTCTATTCAATGTGTGAATGGGGTGATGAGAATCCTGCAACTTGGGCACCCAAGATTGCAAACAGCTGGAGAACTACTGGTGACATTCAAGACAATTGGGACAGCATCACATCTAGAGCTGACATGAATGATAAGTGGGCAGCATATGCTGGGCCTGGTGGATGGAATGATCCTGACATGTTGGAGGTTGGAAATGGCAATATGAGCAACGTTGAATACCGTTCACACTTCAGCATATGGGCTTTGATGAAGGCACCCTTGCTGATTGGATGTGATATAAGGTCTGCTACCTCAGAAACATTAGAGATTTTAAGCAACAGTGAAGTCATTAATGTTAACCAAGACCCATTAGGAGTTCAAGGGAAGAAAGTCAGCCAAGAAGGGGATCTCGAGGTTTGGGCTGGGCAAGTGTCCAATGATAGAGTTGCTGTAATCCTGTGGAACAGAAGCGGTTCACAAGCTGTCATAACTGCCAAATGGGAAGACATTGGCCTTCCCTCTGATGCTATAGTTCAAGCTAGAGACTTGTGGACTCAATCATACCTTCCATCAAATTTACAAAGCAACCTCACATCGATGGTTGATTCGCATGGTGTCAAAATGTACATTCTAACGCCGAGCTCGGGCTAA___ACTCACAGCAAAGCATATCGACAGCTCAATGCCAGGAAATATTAAGAGTTGTAGTTTACACTATCGATTCTGTAATTTTTCTTGGCAATATTTCTATCAAATTTGTGCAAAACCAACCGTGAATGCATGAAGGTTAGAAGGTTGAATGATCTAAAATCAGATCAGAATCAAATAGTTTAACAATAAGGGCATTGCCCTAACAAACACTCTTTAAGCTTATGGAATAAATAATTACTGCATCTTCAATGTTCC

Fln Alignment:

Contig22899___KMVSVSLVVIVFIALWDKTSEGRPIHRHLSSNGLGKTPQMGWNSWNHFGCNIDENIIRETADAxVSSGLSKIGYEYVNIDDCWGELNRDNQGNLVAKASTFPSGIKALADYVHSKGLKFGIYSDAGNYTCGKTMPGSLGYEEQDAKTFASWGVDYLKYDNCFNDDINPKERYPKMSEALTNSGRPIFYSMCEWGDENPATWAPKIANSWRTTGDIQDNWDSITSRADMNDKWAAYAGPGGWNDPDMLEVGNGNMSNVEYRSHFSIWALMKAPLLIGCDIRSATSETLEILSNSEVINVNQDPLGVQGKKVSQEGDLEVWAGQVSNDRVAVILWNRSGSQAVITAKWEDIGLPSDAIVQARDLWTQSYLPSNLQSNLTSMVDSHGVKMYILTPSSG
D5ADT9_______________KMVSVFLVALFLIGFWDQTNGGRPIHRHLSSNGLGKTPQMGWNSWNHFGCNIDENIIRGTADAxVSTGLSKVGYEYVNIDDCWGELNRDAQGNLVAKASTFPSGMKALVDYVHSKGLKLGIYSDAGYYTCSKTMPGSLGYEEQDAKTFASWGIDYLKYDNCFNNGTNPQERYPKMSEALSNAGCPIFYSMCEWGDQNPATWGPKIANSWRTTGDIQDNWDSITSRADQNDQWAAYAGPGGWNDPDMLEVGNGNMSTVEYRSHFSIWALMKAPLLIGCDIRSATSETLEILSNSEVINVNQDPLGVQGKKVSQQGDLEVWAGPLSNNRVAVILWNRSTSQDAITANWGAIGLPSDAIVQARDLWAHSYLPSDLQGSLTSTVDSHDVKMYILTLSSG

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
156	87	12			0.992554
158		12	87		0.992554
159	12		87		0.992554
640			33	66	1.0
853	50	25			1.0
917	83			16	0.994512
971	57	42			1.0
1007		50	50		1.0
1018		20		80	0.999996
1044	10		90		0.90298
1258			40	60	1.0
1268	20			80	0.995493
1274		40	40		1.0
1277	20			80	0.995493
1284	20		80		0.995493
1289	40	60			1.0
1402	53		46		1.0
1447		57		42	1.0
1469		85	14		0.993533
1481	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain