SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene22703

Length: 1271 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene22703 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	NPK1-related protein kinase-like protein [Zea mays]	-	-	2.0e-26	38%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	34%
Sma3	Mitogen-activated protein kinase kinase kinase, putative	-	-	6.506e-07	-

Source	ECs	Term	Type	e value	Identity
Sma3	Mitogen-activated protein kinase kinase kinase.	EC:2.7.11.25	-	2.153e-12	-

Source	Gene names
Sma3	AT4g08480; At4g08480; At4g08500; At5g67080; B0103C08-B0602B01.10; CHLREDRAFT_98709; GSVIVT00000435001; GSVIVT00006868001; GSVIVT00015318001; GSVIVT00027289001; GSVIVT00035304001; GSVIVT00037684001; LOC_Os03g18170; LOC_Os10g04010; MAP3K beta 1; MEKK1; OSJN

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	DNA binding	GO:0003677	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	MAP kinase kinase kinase activity	GO:0004709	Molecular Function	-
Sma3	protein tyrosine kinase activity	GO:0004713	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	kinase binding	GO:0019900	Molecular Function	-
Sma3	MAPK cascade	GO:0000165	Biological Process	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	response to cold	GO:0009409	Biological Process	-
Sma3	response to wounding	GO:0009611	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3		IPR015748	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Source	Species	ID	Description	e value	Identity
RefSeq	Populus trichocarpa	XP_002302871.1	predicted protein, partial [Populus trichocarpa]	9.0e-32	47%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LR19

Fln msg: Unexpected stop codon in the beginning of your sequence, STOP codon was not found. Distance to subject end: 78 aas, your sequence is shorter than subject: 423 - 902

Fln protein: GC*WCEACGFGSAKRIGDDDKGQEALQLRGTPQWMAPEVVNQVEQGPASDIWSLGCTVLEMTTGKHPWGHVSSPLAAMYRIGCTEELPELPASLSLQLRDFLEKCFRRDPKTRWTSAELLNHPFLNEHCSVIEAEEALKCPKSPTSHLDFRNQVWDSYSSEITSILSLNISSPIGEVNGELNRPVKKCPRPSPRDRMMALAAACKFEDVANGPNRITSPHGQWIVVKSSRGNSTTSVSSASSGKSLSKSDISNDCSIQGLPFTKEKCSASLEAVNREELQPRSELNEFLEAVFSAAQPEHQSFSSSSAETLRHDLFSMTAASNLIDEGPDKYENSSQSIAGGDFGNLNISNISVIRKTFHIFDDKWTFGTQGAANCCPCCDSMCATQFCYVNMILGKLLIVCRADTCSCIFYSKLISIDFIQL

Protein Length: 424

Fln nts: TGGGTGCTAATGGTGTGAAGCTTGCGGATTTGGTTCTGCCAAGAGGATTGGTGATGACGATAAGGGCCAGGAGGCCCTGCAATTGAGGGGGACACCTCAGTGGATGGCTCCAGAGGTGGTGAATCAGGTGGAGCAGGGGCCTGCTTCCGATATTTGGTCTCTTGGCTGCACTGTGCTTGAGATGACCACCGGCAAGCATCCATGGGGCCATGTTTCCAGTCCCCTCGCTGCAATGTATCGAATTGGATGCACAGAGGAGCTGCCTGAGTTGCCTGCATCGCTTTCACTTCAACTCCGGGATTTCCTAGAGAAGTGCTTTCGTAGGGATCCCAAGACGAGGTGGACCAGTGCAGAGCTGTTGAACCATCCTTTCCTAAATGAGCACTGCTCTGTCATAGAGGCAGAGGAAGCCCTTAAATGTCCGAAATCTCCAACTAGTCATTTGGATTTCAGGAATCAAGTATGGGATTCCTACTCTTCTGAGATTACAAGCATTCTTTCACTCAACATCTCCAGCCCAATTGGAGAAGTCAATGGGGAGTTGAATAGACCAGTTAAGAAATGCCCAAGGCCTTCTCCCAGGGACAGAATGATGGCACTGGCCGCAGCCTGTAAATTTGAAGATGTTGCTAACGGTCCTAATCGGATCACAAGCCCTCATGGTCAGTGGATTGTTGTGAAATCTTCCAGAGGAAACTCTACAACTTCAGTTTCCTCTGCATCTTCAGGCAAATCTCTGTCAAAATCAGACATCAGTAATGACTGTTCCATCCAAGGGCTTCCATTCACAAAAGAAAAGTGTAGTGCCAGCTTGGAAGCTGTCAACAGGGAAGAGTTGCAGCCCAGATCTGAACTAAATGAATTCCTGGAGGCTGTGTTCTCTGCAGCACAGCCTGAACATCAATCATTTTCCAGTTCTTCTGCTGAGACTTTGCGTCATGACTTGTTTTCAATGACGGCAGCATCCAATTTGATTGATGAAGGCCCTGACAAGTATGAAAATTCATCTCAGAGCATTGCTGGTGGCGATTTTGGAAATCTGAACATATCAAACATATCGGTAATAAGAAAAACATTTCATATCTTTGATGATAAGTGGACCTTTGGTACTCAGGGAGCGGCAAATTGTTGCCCCTGTTGTGATAGTATGTGCGCTACACAATTTTGCTATGTAAATATGATATTGGGGAAATTGCTCATTGTGTGCAGAGCAGATACTTGCTCTTGTATATTTTATTCAAAATTAATTTCAATTGACTTTATTCAGCTAC

Fln Alignment:

Contig22703___FGSAKRIGDDDKGQEALQLRGTPQWMAPEVVNQVEQGP--ASDIWSLGCTVLEMTTGKHPWGHVSSPLAAMYRIGCTXXXXXXXXXXXXXLRDFLEKCFRRDPKTRWTSAELLNHPFLNEHCSVIEAEEALKCPKSPTSHLDFRNQVWDSYSSEITSILSLNISSPIGEVNGELNRPVKKCPRP
B8LR19_______________FGMAKHINGP---ATPLSLKGSPYWMAPEVLMQKNTGHDLAVDIWSLGCTVIEMATGKPPWSEYEG-AAAMFKV-FKSEVPPIPDSLSPEGRHFVQCCLCRNPAQRPKASQLLEHLFVRN----ATQQDSSDLLASATSSIKCLSVVHSPREKNINTFDSTGMSPILHSGNGKQHQPLSENCQP

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
64		50	50		1.0
69		75	25		0.996473
88	25			75	0.996473
89	25			75	0.996473
335		50	50		1.0
424		36	63		1.0
445		63		36	1.0
523		12		87	0.992554
533	50		50		1.0
584		42		57	1.0
658	20			80	0.995493
659			20	80	0.995493
660	80			20	0.995493
662	80		20		0.995493
663	20	20	60		0.99255
668		80		20	0.995493
995	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain