SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene22297

Length: 3101 nt

UniGene Fasta

>sp_v3.0_unigene22297
ATTCCAAAACATGCTTTTGAAATATCTTCACGAAGGTGGCGTGCGCTTACAGGAAGAGGGATCTGTTCTCTCTACTTCCATTTTCCTTTGCGATTCCCGGCGCCCAATTTGCTTCATTGTGTGTTCGAAGGTCTCTGGTTTGCTACGGGTGTTTTCGAGCATATCAGCAGGAAGCATGGGTGATATTTCAGAAATTGGGAATGAAGATGCTGCTGCTCCCAAGTTGCCAATACCGGGGAAGAGGAATGTGTTAATAACGAGTGCACTTCCTTATGTGAACAATGTTCCGCATCTCGGAAATCTGATAGGAAGTGTTTTAAGTGCTGATGTCTTTGCTCGGTATTGTCGGAGGAGGGGGTATAATATGATATATATTTGTGGGACTGATGAATATGGTACAGCCACAGAAACGAAGGCAATGGAAGAGCATTGCACTCCACAACAAATTTGTGATAAATACCATGCCATTCACAAGGAAATATATCAGTGGTTTGATATAAGTTTTGATAAATTTGGTCGTACATCAACACCTGAGCAGACAGAAATCTGCCAGGCAATTTTCAAAACCTTTGGGAGAGAAATTGGCTTTCTGAGAATACGATGCAGCAGCCATATTGTGATACATGCAATCGCTTTTTAGCAGACAGGTTTGTGGAGGGCATTTGCCCCACCCCTGGATGTGGCTATGAGGATGCACGAGGTGATCAGTGTGAAAGTTGTGGCAAACTGTTAAATCCAATAGAACTTTTGAAACCTAGGTGCAAGCTCTGTCGAAATACTCCTCGGATTCGTGATACAGAACACTTATTCTTAGAGCTCCCAAAGTTAAAGGACAGGTTGGAAGAATATATCAAGTTTATGTCGGTTGTTGGTGGTTGGAGTCAAAATGCTGTTCAGGCTACAAATTCTTGGACCAGGGAAGGACTGAAGCCCCGCTGTATTACAAGGGATCTCAAGTGGGGTGTTCCTGTTCCTCTGGAAAAATTTAAAGATAAGGTTTTCTATGTGTGGTTTGATGCTCCGATTGGATACATATCGATAACGGCGTGCTATTCAAAAGATTGGGAAAATTGGTGGAAAAATCCAGAAAATGTGGAGCTTTTTCAGTTTATGGGGAAGGACAATGTTCCATTCCATACGGTCATGTTTCCTTCTACACTCCTTGGAACAGGTGAAAATTGGACTTTGATGAAGACAATTAGTGTTACTGAATACTTAAATTATGAAGCAGGTAAATTTTCTAAAAGCAAAGGGATGGGTGTGTTTGGGAATGATGCTAAAAACACAAATATTCCTGTGGAGGTCTGGCGGTACTATTTGCTGACAAATAGACCTGAGGTGTCAGATACGCTCTTCACATGGGCAGATCTGCAAGCAAAACAAAATAGTGAATTGCTAAAGAATTTAGGCAACTTTGTTCATCGTTCCTTGAGTTTTCTTGCAAAACCTTCAGGTGAAGGTTATGGGTCTATTGTCCCAGATGCCCCAGGTGCAGAGGCACATAAGCTAACTGCGGATCTGGGGGAGAGAGTTGGTAGATTAGTTGAGCAATATGTTGAAGCGATGGAGAAAGTTAAACTTAAACAAGGCTTGAAAACAGCAATGAGCATATCCAGTGAAGGGAATGGTTACCTACAAGATAGTCAATTTTGGAAGCTTTACAAGGACGACCCAGCTGCATGTGCCATTGTAGTGAAAACTTCAACTGGCCTTGTACATCTCTTGGCAACTTTATTGGAACCTTTTATGCCTTCCTTTTCTCATAAGGTGCTGGGGCAGCTCAATCTGCCATGTGAAACATTGTCTCTTTGTGATGAACAAGGTGACGTTGAACAGGCTGGAAAGCCTTGGAACATTGTACCTGCTGGTCACAAGATTGGCAAGCCAGAACCATTATTTTCAGAAATGAAAGACGAGGAGGTCAACTACTACAGAATAAAGTTTTCAGGTAGTCAAGCTGACAGGATTGAGAGGGCTGAAGCTGAAGCATTAAAAGTTGCAGAACAAATCAAAAGATCAACAATTTCAGATAGAGTAGCTGAAAAGGCTGTGGAAGCTAAAATGTCAGCAGAAGCAAAACAAAAAGGGAAAGATTTGCCAATCTCAATAGCAAGGATTGACATTCGAGTTGGCCTTATCACAAATGTTCAGAAACATCCTGATGCAGATTCATTGTATGTGGAAGAGATTGACGTTGGAGAACCAGCTAAAAGGACTGTTGTCAGTGGCCTTGTTAATTATATCCCACTTGAAGAGCTGCAGAATCGGAAGGTGTTAGTAGTATGTAACTTGAAGCCGGCGGCAATGAGAGGGATAAAATCACAGGCTATGGTGTTAGCTGCTTCAAATGATGATCATACCATTGTGGAATTGGTGTCTCCACCTGAGGATGCCCCTGTTGGGGAAAGGTTGAAATTTCCAGGCTTCGATGGTGAACCAGATGAAGTATTGAATCCAAAGAAAAAGATTTGGGAAACTGTGCAACCAGATCTGTATACCACAACTGACCTTATAGCCTGTTACAAAGGTGTTCCTTTTACTACATCTGCAGGGGTCTGCAAAGTTTCCTCTGTCACTTGTGGGTCCATAAAGTAGTTGTTTCTATGAGAAACTTCTGTAGAATATTTGAGAAGCCACTTGTCATGTGACAGAAGCAATCCTAGTCTTTGGAGGGTTTGGTTGAACTGACTGCTGGTTTTTAACTTTTTCATTTAATTCCCTGCAGATCTACTATCCTCTTCTGAATTTTCTTGATGTAGATCTGTTGTTCTTGCTTCCAACATAGAATTTTCAATTCCTTCTAAAATTGTTTCATGCAGTCATGGTCATAGTTGTATAATATTGAGCAGAGTTTCTTTCTTCAAGAAACAAGCTCTGAAATGTTCAAGTCACAAATTTTATGCTTTTTGTGAAATATTTCCAAGTAACTTTCAAGGCAATCTTATAATGAGTATGCAAGGTGTGTTATATCTTTGCAAATACCATTCCATTCAGTTAAATGCAATGTTTCTTCCATTGCGTGTCTATTCTCTAAAATGTCCTGGAATATATTAAGAATCTCTCTGTATCCCATTCAAAACTAAATGCAGAAGATAGCGTTG

Ace file of the UniGene sp_v3.0_unigene22297

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	methionyl-tRNA synthetase [Oryza sativa]	-	-	0.0	71%
FL-Next	sp=Probable methionine--tRNA ligase; Oryza sativa subsp. japonica (Rice).	-	-	0.0	81%
Sma3	Methionyl-tRNA synthetase, putative	-	-	5.958e-12	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:6.1.1.1-	-	3.759e-14	-

Source	Gene names
Sma3	31.t00096; At2g40660; At4g13780; CHLREDRAFT_101947; CHLREDRAFT_104732; CHLREDRAFT_175227; CHLREDRAFT_97144; F18A5.170; GSVIVT00002378001; GSVIVT00008913001; GSVIVT00014805001; GSVIVT00015626001; GSVIVT00029174001; GSVIVT00029457001; GSVIVT00034785001; GSV

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	tRNA binding	GO:0000049	Molecular Function	-
Sma3	aminoacyl-tRNA ligase activity	GO:0004812	Molecular Function	-
Sma3	methionine-tRNA ligase activity	GO:0004825	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	tRNA aminoacylation for protein translation	GO:0006418	Biological Process	-
Sma3	methionyl-tRNA aminoacylation	GO:0006431	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3		IPR002304	-	-
Sma3	tRNA-binding domain	IPR002547	-	-
Sma3	Glutathione S-transferase, C-terminal-like	IPR010987	-	-
Sma3	Nucleic acid-binding, OB-fold	IPR012340	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-
Sma3	Methionyl-tRNA synthetase	IPR014758	-	-
Sma3	Methionyl/Leucyl tRNA synthetase	IPR015413	-	-
Sma3	Glutathione S-transferase/chloride channel, C-terminal	IPR017933	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G13780.1	methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative chr4:7993366-7998433 REVERSE LENGTH=797	69%
RefSeq	Arabidopsis thaliana	NP_193114.1	methionyl-tRNA synthetase [Arabidopsis thaliana]	69%
RefSeq	Populus trichocarpa	XP_002328898.1	predicted protein, partial [Populus trichocarpa]	75%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q9ZTS1

Fln msg: Overlapping hits, possible frame ERROR between 565 and 559, your sequence is longer than subject: 862 - 801

Fln protein: MLLKYLHEGGVRLQEEGSVLSTSIFLCDSRRPICFIVCSKVSGLLRVFSSISAGSMGDISEIGNEDAAAPKLPIPGKRNVLITSALPYVNNVPHLGNLIGSVLSADVFARYCRRRGYNMIYICGTDEYGTATETKAMEEHCTPQQICDKYHAIHKEIYQWFDISFDKFGRTSTPEQTEICQAxxxxLWERNWLSENTMQQPYCDTCNRFLADRFVEGICPTPGCGYEDARGDQCESCGKLLNPIELLKPRCKLCRNTPRIRDTEHLFLELPKLKDRLEEYIKFMSVVGGWSQNAVQATNSWTREGLKPRCITRDLKWGVPVPLEKFKDKVFYVWFDAPIGYISITACYSKDWENWWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGENWTLMKTISVTEYLNYEAGKFSKSKGMGVFGNDAKNTNIPVEVWRYYLLTNRPEVSDTLFTWADLQAKQNSELLKNLGNFVHRSLSFLAKPSGEGYGSIVPDAPGAEAHKLTADLGERVGRLVEQYVEAMEKVKLKQGLKTAMSISSEGNGYLQDSQFWKLYKDDPAACAIVVKTSTGLVHLLATLLEPFMPSFSHKVLGQLNLPCETLSLCDEQGDVEQAGKPWNIVPAGHKIGKPEPLFSEMKDEEVNYYRIKFSGSQADRIERAEAEALKVAEQIKRSTISDRVAEKAVEAKMSAEAKQKGKDLPISIARIDIRVGLITNVQKHPDADSLYVEEIDVGEPAKRTVVSGLVNYIPLEELQNRKVLVVCNLKPAAMRGIKSQAMVLAASNDDHTIVELVSPPEDAPVGERLKFPGFDGEPDEVLNPKKKIWETVQPDLYTTTDLIACYKGVPFTTSAGVCKVSSVTCGSIK*

Protein Length: 863

Fln nts: ATTCCAAAAC_-_ATGCTTTTGAAATATCTTCACGAAGGTGGCGTGCGCTTACAGGAAGAGGGATCTGTTCTCTCTACTTCCATTTTCCTTTGCGATTCCCGGCGCCCAATTTGCTTCATTGTGTGTTCGAAGGTCTCTGGTTTGCTACGGGTGTTTTCGAGCATATCAGCAGGAAGCATGGGTGATATTTCAGAAATTGGGAATGAAGATGCTGCTGCTCCCAAGTTGCCAATACCGGGGAAGAGGAATGTGTTAATAACGAGTGCACTTCCTTATGTGAACAATGTTCCGCATCTCGGAAATCTGATAGGAAGTGTTTTAAGTGCTGATGTCTTTGCTCGGTATTGTCGGAGGAGGGGGTATAATATGATATATATTTGTGGGACTGATGAATATGGTACAGCCACAGAAACGAAGGCAATGGAAGAGCATTGCACTCCACAACAAATTTGTGATAAATACCATGCCATTCACAAGGAAATATATCAGTGGTTTGATATAAGTTTTGATAAATTTGGTCGTACATCAACACCTGAGCAGACAGAAATCTGCCAGGCAxxxxxxxxxxxxCTTTGGGAGAGAAATTGGCTTTCTGAGAATACGATGCAGCAGCCATATTGTGATACATGCAATCGCTTTTTAGCAGACAGGTTTGTGGAGGGCATTTGCCCCACCCCTGGATGTGGCTATGAGGATGCACGAGGTGATCAGTGTGAAAGTTGTGGCAAACTGTTAAATCCAATAGAACTTTTGAAACCTAGGTGCAAGCTCTGTCGAAATACTCCTCGGATTCGTGATACAGAACACTTATTCTTAGAGCTCCCAAAGTTAAAGGACAGGTTGGAAGAATATATCAAGTTTATGTCGGTTGTTGGTGGTTGGAGTCAAAATGCTGTTCAGGCTACAAATTCTTGGACCAGGGAAGGACTGAAGCCCCGCTGTATTACAAGGGATCTCAAGTGGGGTGTTCCTGTTCCTCTGGAAAAATTTAAAGATAAGGTTTTCTATGTGTGGTTTGATGCTCCGATTGGATACATATCGATAACGGCGTGCTATTCAAAAGATTGGGAAAATTGGTGGAAAAATCCAGAAAATGTGGAGCTTTTTCAGTTTATGGGGAAGGACAATGTTCCATTCCATACGGTCATGTTTCCTTCTACACTCCTTGGAACAGGTGAAAATTGGACTTTGATGAAGACAATTAGTGTTACTGAATACTTAAATTATGAAGCAGGTAAATTTTCTAAAAGCAAAGGGATGGGTGTGTTTGGGAATGATGCTAAAAACACAAATATTCCTGTGGAGGTCTGGCGGTACTATTTGCTGACAAATAGACCTGAGGTGTCAGATACGCTCTTCACATGGGCAGATCTGCAAGCAAAACAAAATAGTGAATTGCTAAAGAATTTAGGCAACTTTGTTCATCGTTCCTTGAGTTTTCTTGCAAAACCTTCAGGTGAAGGTTATGGGTCTATTGTCCCAGATGCCCCAGGTGCAGAGGCACATAAGCTAACTGCGGATCTGGGGGAGAGAGTTGGTAGATTAGTTGAGCAATATGTTGAAGCGATGGAGAAAGTTAAACTTAAACAAGGCTTGAAAACAGCAATGAGCATATCCAGTGAAGGGAATGGTTACCTACAAGATAGTCAATTTTGGAAGCTTTACAAGGACGACCCAGCTGCATGTGCCATTGTAGTGAAAACTTCAACTGGCCTTGTACATCTCTTGGCAACTTTATTGGAACCTTTTATGCCTTCCTTTTCTCATAAGGTGCTGGGGCAGCTCAATCTGCCATGTGAAACATTGTCTCTTTGTGATGAACAAGGTGACGTTGAACAGGCTGGAAAGCCTTGGAACATTGTACCTGCTGGTCACAAGATTGGCAAGCCAGAACCATTATTTTCAGAAATGAAAGACGAGGAGGTCAACTACTACAGAATAAAGTTTTCAGGTAGTCAAGCTGACAGGATTGAGAGGGCTGAAGCTGAAGCATTAAAAGTTGCAGAACAAATCAAAAGATCAACAATTTCAGATAGAGTAGCTGAAAAGGCTGTGGAAGCTAAAATGTCAGCAGAAGCAAAACAAAAAGGGAAAGATTTGCCAATCTCAATAGCAAGGATTGACATTCGAGTTGGCCTTATCACAAATGTTCAGAAACATCCTGATGCAGATTCATTGTATGTGGAAGAGATTGACGTTGGAGAACCAGCTAAAAGGACTGTTGTCAGTGGCCTTGTTAATTATATCCCACTTGAAGAGCTGCAGAATCGGAAGGTGTTAGTAGTATGTAACTTGAAGCCGGCGGCAATGAGAGGGATAAAATCACAGGCTATGGTGTTAGCTGCTTCAAATGATGATCATACCATTGTGGAATTGGTGTCTCCACCTGAGGATGCCCCTGTTGGGGAAAGGTTGAAATTTCCAGGCTTCGATGGTGAACCAGATGAAGTATTGAATCCAAAGAAAAAGATTTGGGAAACTGTGCAACCAGATCTGTATACCACAACTGACCTTATAGCCTGTTACAAAGGTGTTCCTTTTACTACATCTGCAGGGGTCTGCAAAGTTTCCTCTGTCACTTGTGGGTCCATAAAGTAG___TTGTTTCTATGAGAAACTTCTGTAGAATATTTGAGAAGCCACTTGTCATGTGACAGAAGCAATCCTAGTCTTTGGAGGGTTTGGTTGAACTGACTGCTGGTTTTTAACTTTTTCATTTAATTCCCTGCAGATCTACTATCCTCTTCTGAATTTTCTTGATGTAGATCTGTTGTTCTTGCTTCCAACATAGAATTTTCAATTCCTTCTAAAATTGTTTCATGCAGTCATGGTCATAGTTGTATAATATTGAGCAGAGTTTCTTTCTTCAAGAAACAAGCTCTGAAATGTTCAAGTCACAAATTTTATGCTTTTTGTGAAATATTTCCAAGTAACTTTCAAGGCAATCTTATAATGAGTATGCAAGGTGTGTTATATCTTTGCAAATACCATTCCATTCAGTTAAATGCAATGTTTCTTCCATTGCGTGTCTATTCTCTAAAATGTCCTGGAATATATTAAGAATCTCTCTGTATCCCATTCAAAACTAAATGCAGAAGATAGCGTTG

Fln Alignment:

Contig22297___PKLPIPGKRNVLITSALPYVNNVPHLGNLIGSVLSADVFARYCRRRGYNMIYICGTDEYGTATETKAMEEHCTPQQICDKYHAIHKEIYQWFDISFDKFGRTSTPEQTEICQAIxxNLWERNWLSENTMQQPYCDTCNRFLADRFVEGICPTPGCGYEDARGDQCESCGKLLNPIELLKPRCKLCRNTPRIRDTEHLFLELPKLKDRLEEYIKFMSVVGGWSQNAVQATNSWTREGLKPRCITRDLKWGVPVPLEKFKDKVFYVWFDAPIGYISITACYSKDWENWWKNPENVELFQFMGKDNVPFHTVMFPSTLLGTGENWTLMKTISVTEYLNYEAGKFSKSKGMGVFGNDAKNTNIPVEVWRYYLLTNRPEVSDTLFTWADLQAKQNSELLKNLGNFVHRSLSFLAKPSGEGYGSIVPDAPGAEAHKLTADLGERVGRLVEQYVEAMEKVKLKQGLKTAMSISSEGNGYLQDSQFWKLYKDDPAACAIVVKTSTGLVHLLATLLEPFMPSFSHKVLGQLNL-PCETLSLCDEQGDVEQAGKPWNIVPAGHKIGKPEPLFSEMKDEEVNYYRIKFSGSQADRIER--AEAEALKVAEQIKRSTISDRVAEKAVEAKMSAEAKQKGKDLPISIARIDIRVGLITNVQKHPDADSLYVEEIDVGEPAKRTVVSGLVNYIPLEELQNRKVLVVCNLKPAAMRGIKSQAMVLAASNDDHTIVELVSPPEDAPVGERLKFPGFDGEPDEVLNPKKKIWETVQPDLYTTTDLIACYKGVPFTTSAGVCKVSSVTCGSIK
Q9ZTS1_______________PKLPVPGRRNILVTSALPYVNNVPHLGNIIGCVLSADVFARYCRLRGYNVIYICGTDEYGTATETKAMEEKCSPKEICDKYHAVHSEVYKWFDIKFDKFGRTSSPQQTEVCQAIxxKLMENNWLTENTMQQLYCDTCQRFLADRLVEGKCPTEGCNYEAARGDQCENCSKLLNPTELIDPKCKVCKNTPRVRDTDHLFLELPLLSDKLVNYINETSVAGMWSQNAIQATNAWLKEGLKPRCITRDLKWGVPVPHEKYKDKVFYVWFDAPIGYVSITASYTPDWEKWWKDPDNVELFQFMGKDNVPFHTVMFPSTLLGTGEKWTMMKTISVTEYLNYEAGKFSKSHGIGVFGNDAKDTNIPPEVWRYYLLTNRPEVSDTLFTWADLQAKLNSELLNNLGNFINRVLSFVAKPAGAGYDSIVPDAPNAESHPLTKALVEKTNKWVEQYLEAMEKVKLKQGLKSAMGISSDGNAYLQESQFWKLYKEDPAACAVVMKTSVGVVYLLACLLEPFMPSFSNEVLLQLNMTPEESLSFCDDKGEIAKAKRPWDFVSAGHKIGKPSPLFKELKDEEVESFRNKFAGSQAERSSKAQADAEAKKVADKLKGTKLSDG-GQKKEQKKQSGGSKSKNAEVDVTVAKLDIRVGLIRKAQKHPDADSLYVEEIDVGEEAPRTVVSGLVKFIPLEEMQNRKVCVLCNLKPVAMRGIKSHAMVLAASNEDHTKVELVEPPESAAVGERVTFAGYSGEPEASLNAKSKTWEKLSADLHSNGELVACYKDVPFTTSAGVCKVKSIASGEIR

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
781		12		87	0.992554
841	12		87		0.992554
1016	16		83		0.994512
1023	20	80			0.995493
1243	20			80	0.995493
1244	75	25			0.996473
1246	75			25	0.996473
1247	75			25	0.996473
1263	20			80	0.995493
1264	20		80		0.995493
1266			20	80	0.995493
1435		25		75	0.996473
1597	87			12	0.992554
1598	87		12		0.992554
1599		87		12	0.992554
1601			87	12	0.992554
1602		87		12	0.992554
1657	12		87		0.992554
1659	12			87	0.992554
1991			33	66	1.0
2211	85	14			0.993533
2213	85			14	0.993533
2214			85	14	0.993533
2304		83		16	0.994512
2519	11			88	0.999937
2940	75		25		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain