SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene21808

Length: 810 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene21808 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam00150, Cellulase, Cellulase (glycosyl hydrolase family 5)	-	-	4.0e-06	18%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	48%
Sma3	(1-4)-beta-mannan endohydrolase	-	-	1.025e-13	-

Source	ECs	Term	Type	e value	Identity
Sma3	Mannan endo-1,4-beta-mannosidase.	EC:3.2.1.78	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Fructose and mannose metabolism	00051		0.0	%

Source	Gene names
Sma3	At1g02310; At2g20680; At3g10890; At3g10900; At3g30540; At4g28320; At5g01930; At5g66460; F26K10.200; F5H14.35; GSVIVT00010891001; GSVIVT00014580001; GSVIVT00018590001; GSVIVT00024894001; GSVIVT00032044001; GSVIVT00032045001; GSVIVT00032046001; GSVIVT000320

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	hydrolase activity	GO:0016787	Molecular Function	-
Sma3	mannan endo-1,4-beta-mannosidase activity	GO:0016985	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Bulb-type lectin domain	IPR001480	-	-
Sma3	Glycoside hydrolase, family 5	IPR001547	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G66460.1	MAN7, AtMAN7 Glycosyl hydrolase superfamily protein chr5:26538911-26540837 REVERSE LENGTH=431	57%
RefSeq	Arabidopsis thaliana	NP_201447.1	mannan endo-1,4-beta-mannosidase 7 [Arabidopsis thaliana]	57%
RefSeq	Populus trichocarpa	XP_002310816.1	predicted protein [Populus trichocarpa]	62%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A9Z9

Fln msg: STOP codon was not found. Distance to subject end: 15 aas, your sequence is shorter than subject: 269 - 429

Fln protein: RRLWGRAQYVQWGRDAGQNMRSDDDFYTNPTVKGYYKNHVKTVITRVNSISGIAYKDDPTIFSWELINEPRCQIDPSGRTLQGWITEMAAYVKSTDSNHLLEVGMEGFYGESTPERKQYNPGGYEYGTDFITNNQIPGIDFATVHAYPDHWLAGSDENSQLSFLNKWMQIHFYDVERVLNKPLLFAEFGKSYKDPGYTTAQRDRFYNTVYNNIRSALSSGKAGGGGLFWQLLAEGMDSFADGYDIVLSRNPSIAAIIASQSHRLSLLNT

Protein Length: 270

Fln nts: GCCGAAGATTATGGGGAAGGGCACAATACGTTCAGTGGGGACGCGATGCAGGGCAGAATATGAGGTCCGATGATGATTTCTACACTAATCCCACCGTGAAAGGTTACTACAAGAACCATGTCAAGACTGTAATAACGAGAGTGAATAGTATCAGTGGAATTGCTTACAAGGATGACCCAACAATATTTTCATGGGAGCTCATCAACGAACCCCGCTGCCAAATTGACCCTTCTGGAAGGACTTTGCAGGGATGGATAACGGAAATGGCCGCCTACGTGAAATCGACTGATAGCAACCACCTTCTTGAAGTAGGCATGGAAGGATTCTATGGCGAATCGACCCCTGAAAGAAAGCAGTACAATCCAGGCGGTTACGAATATGGAACAGACTTCATTACTAACAATCAGATTCCAGGAATTGATTTTGCAACTGTTCATGCCTATCCAGATCATTGGTTGGCTGGATCAGATGAAAATTCACAGCTTTCCTTCTTGAACAAATGGATGCAAATCCATTTCTATGATGTGGAAAGAGTGCTGAACAAACCACTGCTGTTTGCTGAATTCGGGAAATCATATAAAGATCCAGGTTATACCACTGCTCAGAGGGACAGGTTTTATAATACTGTCTATAACAACATTCGCAGCGCTCTGAGCAGTGGAAAAGCAGGGGGAGGAGGCCTATTCTGGCAGTTACTGGCAGAAGGGATGGACTCGTTTGCAGATGGGTATGATATTGTCCTGTCCCGAAATCCATCCATAGCTGCTATTATTGCTTCTCAGTCGCATAGATTGTCTCTCTTAAACACCA

Fln Alignment:

Contig21808___GRAQYVQWGRDAGQNM-RSDDDFYTNPTVKGYYKNHVKTVITRVNSISGIAYKDDPTIFSWELINEPRCQIDPSGRTLQGWITEMAAYVKSTDSNHLLEVGMEGFYGESTPERKQYNPGGY--EYGTDFITNNQIPGIDFATVHAYPDHWLAGSDENSQLSFLNKWMQIHFYDVERVLNKPLLFAEFGKSYKDPGYTTAQRDRFYNTVYNNIRXXXXXXXXXXXXLFWQLLAEGMDSFADGYDIVLSRNPSIAAIIASQSHRLSLL
D5A9Z9_______________GKVQYVEWAHAAGINVGSSNDSFFSDPTIRGYYKEYVKSILVRKNSISGVEYRNEPAIFAWELVNEPRCTSDASGQTLQTWISEMAKFIKSLDSRHLLTVGLEGFYGKTTPNKIELNPGDWAVSLGSDFIWNSQVEEIDFASVHAYPDSWIPNVDLEDHLNYFSKWVTSHIDDGEQILKKPVLFTEFGLSSHHKGFEQSHRDRLFKTMYEEIDESARKGGAGGGALIWQFVVEGMEEYGDEFAFVPWQFPSTHKLIVEQSCRLQTL

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
224			33	66	0.997452
245	37		62		1.0
404		66		33	1.0
463			50	50	1.0
471	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain