SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene21775

Length: 2184 nt

UniGene Fasta

>sp_v3.0_unigene21775
ACGTGACAACCCTTGAATGGTCAGTATAGTGATTTGTGGGAACTGAGGAAGTCTAGAGGCAGCTTGGGCTGGAATGGGAAATATACTCAATTGCTCTAAAATCACATCCCATTCTATTCATTCAATTAAAATCCGTCCAAGTCGCAAACCAGCAATGGCGCAAAACAGAGGATGCCTTGTTTGCTGCCCTGTCATATCACGTTCAGTGGAAGAGACACTGGAGCAAATGCAACAAGCCAAAGTTGATGGTGCAGACATCGTTGAGATCAGGATTGACCTCTTAAATAACTTCAATCCAAAGGCTGATTTAGCAATTCTGCTCAAAGAAAGACCACTGCCCATCATCATTACTTACAGGCCAAAGTGGGAGGGAGGTGAGTATGAAGGTGATGAAGCAACACGCCTTGATGTACTTCGTTTGGCTATAGAATTGGATGCCGACTATGTTGATGTGGAGCTCCAGGTGGCAACCGATTTCATAAAAAGCATGCGTGACAAGAAATCAAGAATGTGCAAGATCATTGTTTCGAATCATAATTTTGAAAGTACTCCATCAGTTGAGGAACTTTGTCATCTTGTTGAAAGTTTACAATCTACAGGAGCTGACATTGTGAAATTTACGACAACTGCCACTACTATTACTGACGTTGCCCGGGTTTTTCAAGTGTTTGCTTACTCTCAAGTTCCCATTATTGCATCGGTGATTTCTTCCAGAGGGCTAATTAGCTCGATTCTTTGCCCAAAGTTTGGTGGATATCTTACGTTTGGCTCCTTGGGACCAGGGAAGGAATCAGCTTCGGGTCAACCAGCATTGACGGATCTAATAAACATATACAGGATAAAGAACTTGGGTAAAGATACCAAGGTTTTTTGGAATCATAGGAAATCCAGTAGGTCACAGCAAAGGACCAATTCTGCACAATCGGGCATTCAGAGAGATAGGACTTGATGCAGTCTATGTACCTTTTCTTGTTGATGATATTAAAGAGTTTCTAAAAGTCTATTCATCTCCAGACTTCGCTGGTTTCAGTGTTACCATTCCTTACAAGGAGGCTGCTCTTGAATGTTGTGACGAAGTTGATCCAATTGCACGGTCTATTGGAGCAGTAAATACAATAGTGAGGAGGCCAAGTGATGGAAAACTTATTGGGTATAACACAGACTATACAGGTGCCATTTCTGCAATTGAGGATGGACTAAAAGGTAGACTTAGGAGTGGAGACTGGGGAAATACTTCCCCTCTTTCTGGAAGACTCTTCGTGGTTGTTGGAGCTGGAGGAGCTGGTAAAGCCCTTGCATATGGGGCAACAGAAAAGGGAGCGAGGGTTGTGATTGCTAATCGTAATTATGAAAGGGCAAAGGCACTGGCGAATTTAGTTGGAGGGGAAGCTATCCCTCTAGACAAGTTGGACAGCTTTTGTCAAGAGAATGGAATGATTCTTGCAAACACTACTTCTGTTGGAATGCATCCAAATGTGATGGAGACACCTATATCAAAGGAGGCTTTAGGGGCATACGATCTTGTTTTTGATGCTGTTTACACACCAAAAGTTACTAGACTCTTACGAGAAGCAGAAGAGGCTGGGGCTACTGTTGTAAGCGGGTTGGAAATGTTTATAAGGCAGGCCATGGGTCAATTTGAACTCTTTACTGGTACTCCAGCACCTGGGGTATTAATGCGGGAAATCGTTGCCAGACTGTAAGACATGCTTGGGCAGCTTTTGGAGATATATGTAGCTGCGATTATTTGGAGCTTGTTGGTGCTACATTGTGCTTCAGTTCTGAAAGGTTCTTTGGAATGACATTGTCTTTTGGAAAAAAAATTCTGTGCTCTATTGACATTTGTAGGTCATAATTCTTCTAGATAAAATTATTTGTTCTCGTGATAAATTATGGGAGACATATGAAGTGCATAGTTGAAGCAGAAGTGGATCATATGTGAAAGTTATCTATGCTTTTGGAATCCTTTTAGCTAGTAGTGTTATAAAAGTGTTTTTTCACTTTACAAATAAAGTCTCTTAGGCATCTTGACAACACTCTCAAGATACAAATACTAGAGCTGTGAGATAATCTTTAATATTTTAGTTGAATGAAACCGCAAGGTGATGAAAAATTGGCTAATAAAGGGTGTGTGGATGCTTATGAAATGTAATGCAATAAAATAAGGTGGTCTCATCATTCACG

Ace file of the UniGene sp_v3.0_unigene21775

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	3-dehydroquinate dehydratase/shikimate 5-dehydrogenase [Juglans regia]	-	-	0.0	63%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	66%
Sma3	Dehydroquinate dehydratase/ shikimate dehydrogenase	-	-	7.226e-19	-

Source	ECs	Term	Type	e value	Identity
Sma3	Shikimate dehydrogenase.	EC:1.1.1.25	-	0.0	-

Source	KEGGs	Term	Type	Identity
Sma3	Phenylalanine, tyrosine and tryptophan biosynthesis	00400		%
Sma3	Biosynthesis of phenylpropanoids	01061		%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063		%
Sma3	Biosynthesis of plant hormones	01070		%
Sma3	Metabolic pathways	01100		%
Sma3	Biosynthesis of secondary metabolites	01110		%
Sma3		EC:4.2.1.1-	-	-

Source	Gene names
Sma3	At3g06350; B1111C09.6; B1111C09.8; CHLREDRAFT_196935; DHQD1; DHQD2; DHQD3; DHQD4; DHQD5; EMB3004; F24P17.18; GSVIVT00008899001; GSVIVT00008900001; GSVIVT00008901001; GSVIVT00019835001; MICPUCDRAFT_1820; MICPUN_89537; OSTLU_39392; Os01g0375200; Os01g037540

Source	GOs	Term	Type	Identity
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	3-dehydroquinate dehydratase activity	GO:0003855	Molecular Function	-
Sma3	shikimate 3-dehydrogenase (NADP+) activity	GO:0004764	Molecular Function	-
Sma3	binding	GO:0005488	Molecular Function	-
Sma3	NADP binding	GO:0050661	Molecular Function	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	aromatic amino acid family biosynthetic process	GO:0009073	Biological Process	-
Sma3	embryo development ending in seed dormancy	GO:0009793	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Dehydroquinase class I	IPR001381	-	-
Sma3	Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase	IPR006151	-	-
Sma3	Shikimate dehydrogenase	IPR011342	-	-
Sma3	Shikimate dehydrogenase substrate binding, N-terminal	IPR013708	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	3-dehydroquinate dehydratase, active site	IPR018508	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G06350.1	EMB3004, MEE32 dehydroquinate dehydratase, putative / shikimate dehydrogenase, putative chr3:1924536-1927701 REVERSE LENGTH=603	64%
RefSeq	Arabidopsis thaliana	NP_187286.1	Shikimate dehydrogenase [Arabidopsis thaliana]	64%
RefSeq	Populus trichocarpa	XP_002314474.1	dehydroquinate dehydratase/ shikimate dehydrogenase [Populus trichocarpa]	67%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LLR2

Fln msg: Overlapping hits, possible frame ERROR between 871 and 864,

Fln protein: MGNILNCSKITSHSIHSIKIRPSRKPAMAQNRGCLVCCPVISRSVEETLEQMQQAKVDGADIVEIRIDLLNNFNPKADLAILLKERPLPIIITYRPKWEGGEYEGDEATRLDVLRLAIELDADYVDVELQVATDFIKSMRDKKSRMCKIIVSNHNFESTPSVEELCHLVESLQSTGADIVKFTTTATTITDVARVFQVFAYSQVPIIASVISSRGLISSILCPKFGGYLTFGSLGPGKESASGQPALTDLINIYRIKNLGKDTxxxxGIIGNPVGHSKGPILHNRAFREIGLDAVYVPFLVDDIKEFLKVYSSPDFAGFSVTIPYKEAALECCDEVDPIARSIGAVNTIVRRPSDGKLIGYNTDYTGAISAIEDGLKGRLRSGDWGNTSPLSGRLFVVVGAGGAGKALAYGATEKGARVVIANRNYERAKALANLVGGEAIPLDKLDSFCQENGMILANTTSVGMHPNVMETPISKEALGAYDLVFDAVYTPKVTRLLREAEEAGATVVSGLEMFIRQAMGQFELFTGTPAPGVLMREIVARL*

Protein Length: 545

Fln nts: ACGTGACAACCCTTGAATGGTCAGTATAGTGATTTGTGGGAACTGAGGAAGTCTAGAGGCAGCTTGGGCTGGA_-_ATGGGAAATATACTCAATTGCTCTAAAATCACATCCCATTCTATTCATTCAATTAAAATCCGTCCAAGTCGCAAACCAGCAATGGCGCAAAACAGAGGATGCCTTGTTTGCTGCCCTGTCATATCACGTTCAGTGGAAGAGACACTGGAGCAAATGCAACAAGCCAAAGTTGATGGTGCAGACATCGTTGAGATCAGGATTGACCTCTTAAATAACTTCAATCCAAAGGCTGATTTAGCAATTCTGCTCAAAGAAAGACCACTGCCCATCATCATTACTTACAGGCCAAAGTGGGAGGGAGGTGAGTATGAAGGTGATGAAGCAACACGCCTTGATGTACTTCGTTTGGCTATAGAATTGGATGCCGACTATGTTGATGTGGAGCTCCAGGTGGCAACCGATTTCATAAAAAGCATGCGTGACAAGAAATCAAGAATGTGCAAGATCATTGTTTCGAATCATAATTTTGAAAGTACTCCATCAGTTGAGGAACTTTGTCATCTTGTTGAAAGTTTACAATCTACAGGAGCTGACATTGTGAAATTTACGACAACTGCCACTACTATTACTGACGTTGCCCGGGTTTTTCAAGTGTTTGCTTACTCTCAAGTTCCCATTATTGCATCGGTGATTTCTTCCAGAGGGCTAATTAGCTCGATTCTTTGCCCAAAGTTTGGTGGATATCTTACGTTTGGCTCCTTGGGACCAGGGAAGGAATCAGCTTCGGGTCAACCAGCATTGACGGATCTAATAAACATATACAGGATAAAGAACTTGGGTAAAGATACCxxxxxxxxxxxxGGAATCATAGGAAATCCAGTAGGTCACAGCAAAGGACCAATTCTGCACAATCGGGCATTCAGAGAGATAGGACTTGATGCAGTCTATGTACCTTTTCTTGTTGATGATATTAAAGAGTTTCTAAAAGTCTATTCATCTCCAGACTTCGCTGGTTTCAGTGTTACCATTCCTTACAAGGAGGCTGCTCTTGAATGTTGTGACGAAGTTGATCCAATTGCACGGTCTATTGGAGCAGTAAATACAATAGTGAGGAGGCCAAGTGATGGAAAACTTATTGGGTATAACACAGACTATACAGGTGCCATTTCTGCAATTGAGGATGGACTAAAAGGTAGACTTAGGAGTGGAGACTGGGGAAATACTTCCCCTCTTTCTGGAAGACTCTTCGTGGTTGTTGGAGCTGGAGGAGCTGGTAAAGCCCTTGCATATGGGGCAACAGAAAAGGGAGCGAGGGTTGTGATTGCTAATCGTAATTATGAAAGGGCAAAGGCACTGGCGAATTTAGTTGGAGGGGAAGCTATCCCTCTAGACAAGTTGGACAGCTTTTGTCAAGAGAATGGAATGATTCTTGCAAACACTACTTCTGTTGGAATGCATCCAAATGTGATGGAGACACCTATATCAAAGGAGGCTTTAGGGGCATACGATCTTGTTTTTGATGCTGTTTACACACCAAAAGTTACTAGACTCTTACGAGAAGCAGAAGAGGCTGGGGCTACTGTTGTAAGCGGGTTGGAAATGTTTATAAGGCAGGCCATGGGTCAATTTGAACTCTTTACTGGTACTCCAGCACCTGGGGTATTAATGCGGGAAATCGTTGCCAGACTGTAA___GACATGCTTGGGCAGCTTTTGGAGATATATGTAGCTGCGATTATTTGGAGCTTGTTGGTGCTACATTGTGCTTCAGTTCTGAAAGGTTCTTTGGAATGACATTGTCTTTTGGAAAAAAAATTCTGTGCTCTATTGACATTTGTAGGTCATAATTCTTCTAGATAAAATTATTTGTTCTCGTGATAAATTATGGGAGACATATGAAGTGCATAGTTGAAGCAGAAGTGGATCATATGTGAAAGTTATCTATGCTTTTGGAATCCTTTTAGCTAGTAGTGTTATAAAAGTGTTTTTTCACTTTACAAATAAAGTCTCTTAGGCATCTTGACAACACTCTCAAGATACAAATACTAGAGCTGTGAGATAATCTTTAATATTTTAGTTGAATGAAACCGCAAGGTGATGAAAAATTGGCTAATAAAGGGTGTGTGGATGCTTATGAAATGTAATGCAATAAAATAAGGTGGTCTCATCATTCACG

Fln Alignment:

Contig21775___CLVCCPVISRSVEETLEQMQQAKVDGADIVEIRIDLLNNFNPKADLAILLKERPLPIIITYRPKWEGGEYEGDEATRLDVLRLAIELDADYVDVELQVATDFIKSMRDKKSRMCKIIVSNHNFESTPSVEELCHLVESLQSTGADIVKFXXXXXXXXDVARVFQVFAYSQVPIIASVISSRGLISSILCPKFGGYLTFGSLGPGKESASGQPALTDLINIYRIKNLGKDTxxxGIIGNPVGHSKGPILHNRAFREIGLDAVYVPFLVDDIKEFLKVYSSPDFAGFSVTIPYKEAALECCDEVDPIARSIGAVNTIVRRPSDGKLIGYNTDYTGAISAIEDGLKGRLRSGDWGNTSPLSGRLFVVVXXXXXXXXXXXXXTEKGARVVIANRNYERAKALANLVGGEAIPLDKLDSFCQENGMILANTTSVGMHPNVMETPISKEALGAYDLVFDAVYTPKVTRLLREAEEAGATVVSGLEMFIRQAMGQFELFTGTPAPGVLMREIVAR
B8LLR2_______________CLLCIPLVGQSMEEMLSQMELARIHGADCVELRIDHVRNFNPRVDLDTLLKARTMPAIVTYRPKWEGGAYEGDEGKRLDVLRLAMELGADYIDVELQVASDFIKSISNKKRENSKIIVSNHNYQFTPSLEDLGLLVARIQSTGADIVKIATTATDITDVARIFRVLAYCQVPILALVMSERGLISRLLCPKFGGYLTFGSLEAGKESAPGQATLADMLNIYKIRGLGRDTxxxGLIGNPVSHSKGPTLHNRAFGEIGFDAIYVPFLVDDIKEFLKVYSSPDFAGFSVTIPHKEAALECCDEVDPIARSIGAVNTIVRRQSDGKLIGYNTDSVGAISAIEDALKGKHNYGDWTHSSPLAGKLFLIVGAGGAGKALAFGAKQKGARVVIANRNYERAKALAIQVEGEAIPLDKLDHFRPETGMILANTTSMGMHPNVSQTPISKEALSSYAVVFDAVYTPKWTRLLQDAEATGALVVCGLEMFIRQAMGQFELFTGCKAPEKLMREIVLK

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
346		66	33		0.997452
347	66		33		0.997452
348			33	66	0.997452
569		75		25	0.996473
570			25	75	0.996473
605	16		83		0.994512
606	16	83			0.994512
609	83			16	0.994512
686	14		85		0.993533
729	16		83		0.994512
732		83		16	0.994512
1972			18	81	0.999994
2074		33	66		1.0
2111	16	83			0.994512
2112	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain