SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene21674

Length: 2205 nt

UniGene Fasta

>sp_v3.0_unigene21674
CCTAATTTTTTCGCTACGAATCGATCTTTTCTCTACAAGGGTTTGAAGGATCGTTTCGGCGTTTTTCTCGTCTGTAAATTCTTCGCTCCGAATCCATCTTTTTGCTACAAGGGTTTCGTTTCGGCGTTTTTCTCGTCTGTAACTTCTTCGCTCCGAATCCATCTTTTCTCTACAAGGGTTTGAAGGCTCGTTTCGGTGTTTTTCTCGTGTTTTCGTTAAGCTTTCGAGGAGAAATCATGGCGGAAGCTGGTGAAGTTGAGACCTTTGCCTTCCAGGCTGAGATCAACCAGCTTTTGAGTCTTATTATCAACACTTTTTACAGCAACAAGGAAATTTTTCTTAGAGAGATCATCAGCAATTCCTCAGATGCTTTGGACAAGATCCGGTTTGAGAGTCTCACGGACAAGAGTAAGCTGGATGCACAACCAGAACTGTTTATTCACATTATTCCAGACAAGACAAACAATTCGTTGACTATTATCGACAGTGGTATTGGCATGACCAAATCTGATCTCGTGAACAACCTGGGAACCATTGCACGATCTGGCACGAAAGAGTTTATGGAAGCTCTTGCTGCTGGTGCTGATGTTAGCATGATTGGTCAGTTTGGTGTGGGTTTCTACTCTGCGTATTTGGTTGCTGAGAAGGTGCTTGTCACCACCAAACACAATGACGATGAGCAATATATTTGGGAATCGCAGGCCGGCGGATCGTTTACTGTTACCAGAGACACAAGTGGGGAGGTTCTTGGTAGAGGAACCAAAATCACACTGTTGCTCAAAGATGATCAGCTGGAATACCTCGAGGAGAGGCGTTTGAAGGACCTTACTAAGAAGAATTCACGCGAGACTCACCAACAAAACAATATCTCTCTGGACTGAGAAGACTACAGAGAAGGAAGTTTCAGATGATGAGGAGGAGGAGGAAAAGGAAAAGAAAGAGGAAGAAGGCACTGTTGAGGAAGTGGATGAAGAAAAGGAGAAGGAAGAGAAGAAGAAGAAGAAAATCAAGGAAGTTTCACATGAATGGAGTCTTGTGAACAAACAGAAGCCTATCTGGATGAGAAAACCAGAGGATATTACGAAGGAAGAATATGCGGCATTTTACAAGAGTCTGACAAATGACTGGGAAGAACACTTGGCAGTGAAACACTTCTCTGTTGAGGGACAGCTTGAGTTCAAGGCCGTCCTCTTTGTTCCCAAGAGGGCACCGTTTGATCTCGTCGATACCAAGAAGAAGTTAAATAACATCAAGCTCTATGTCAGGCGTGTATTCATCATGGACAACTGTGAAGAGTTGATCCCGGAATACCTTAGCTTTGTAAAGGGTGTCGTGGACTCTGAAGACCTTCCTTTGAATATTTCTCGCGAGACTCTCCAACAAAACAAAATCCTAAAAGTTATTAGGAAGAATCTTGTCAAGAAGTGTGTAGAGTTGTTCTTTGAAATTGCTGAAAACAAGGAGGATTACAATAAATTCTACGAGGCATTTTCAAAGAACCTTAAGCTTGGGATTCATGAGGATAGCCAGAACAGATCCAAGATTGCTGACTTGCTCAGGTACCACTCTACTAAGAGTGGAGATGAGATGACCAGCTTGAAGGATTATGTGACAAGAATGAAGGAGGGTCAAAAGGATATCTATTACATAACTGGTGAAAGCAAGAAGGCAGTTGAGAACTCTCCATTCTTGGAGCGTCTGAAGAAGAAGGGTTACGAAGTTCTTTTCATGGTTGAGGCTATTGATGAATATGCCATTGGGCAATTGAAGGAATACGAAGGCAAAAAATTGGTGTCTGCCACAAAGGAGGGGTTGAAAAAAGGAAGAGTTGAAGTCCCTATTTGATGGTCTCTGCAAAGTTATCAAGGATATTCTCGGGGACAAGGTGGAGAAAGTTGTCGTCTCTGACCGTGTTGTGGATTCTCCTTGCTGTTTGGTTACTGGAGAGTATGGATGGACTGCTAACATGGAAAGAATCATGAAGGCCCAGGCTTTGAGAGACAGTAGCATGTCAAGTTACATGTCAAGCAAGAAGACCATGGAAATTAATCCCGAGAATCCTATCATGGATGAGCTTAGAAAAATGACAAGTCTGTCAAGGATTTAGTTCTATTGATGTACGAAACTGCTTTGTTGACATCAGGGTTTAGCCTTGATGATCCTAATACTTTTGGTAACAGGATTCACAGGATGATGAAGCTGGGT

Ace file of the UniGene sp_v3.0_unigene21674

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Heat shock protein 81-1 n=55 Tax=Magnoliophyta RepID=HSP81_ORYSJ	-	-	94%
FL-Next	tr=GRP94; Pinus taeda (Loblolly pine).	-	-	59%
Sma3	Heat shock protein 90	-	-	-

Source	Gene names
Sma3	At5g52640; At5g56000; At5g56010; At5g56030; CHLREDRAFT_138117; GSVIVT00004280001; HSC80; HSP81-1; HSP81-2; HSP81-3; HSP81.2; HSP82; HSP83; HSP83A; HSP90; HSP90-1; HSP90-2; HSP90A; HSP90_1; Hsp90; LOC_Os09g30438; MDA7.5; MDA7.7; MICPUCDRAFT_26319; MICPUN_1

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	nucleolus	GO:0005730	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	unfolded protein binding	GO:0051082	Molecular Function	-
Sma3	protein folding	GO:0006457	Biological Process	-
Sma3	response to stress	GO:0006950	Biological Process	-
Sma3	defense response	GO:0006952	Biological Process	-
Sma3	response to water deprivation	GO:0009414	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	flower development	GO:0009908	Biological Process	-
Sma3	heat acclimation	GO:0010286	Biological Process	-
Sma3	leaf development	GO:0048366	Biological Process	-
Sma3	protein stabilization	GO:0050821	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Heat shock protein Hsp90	IPR001404	-	0.0	-
Sma3	ATPase-like, ATP-binding domain	IPR003594	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G56030.2	HSP81-2 heat shock protein 81-2 chr5:22686923-22689433 FORWARD LENGTH=728	90%
RefSeq	Arabidopsis thaliana	NP_001190553.1	heat shock protein 81-2 [Arabidopsis thaliana]	90%
RefSeq	Populus trichocarpa	XP_002329963.1	predicted protein [Populus trichocarpa]	91%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A7YAU9

Fln msg: Separated hits, possible frame ERROR between 842 and 1018, Unexpected STOP codon at 3' end. Distance to subject end: 76 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 537 - 834

Fln protein: MAEAGEVETFAFQAEINQLLSLIINTFYSNKEIFLREIISNSSDALDKIRFESLTDKSKLDAQPELFIHIIPDKTNNSLTIIDSGIGMTKSDLVNNLGTIARSGTKEFMEALAAGADVSMIGQFGVGFYSAYLVAEKVLVTTKHNDDEQYIWESQAGGSFTVTRDTSGEVLGRGTKITLLLKDDQLEYLEERRLKDLTKKNxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSHEWSLVNKQKPIWMRKPEDITKEEYAAFYKSLTNDWEEHLAVKHFSVEGQLEFKAVLFVPKRAPFDLVDTKKKLNNIKLYVRRVFIMDNCEELIPEYLSFVKGVVDSEDLPLNISRETLQQNKILKVIRKNLVKKCVELFFEIAENKEDYNKFYEAFSKNLKLGIHEDSQNRSKIADLLRYHSTKSGDEMTSLKDYVTRMKEGQKDIYYITGESKKAVENSPFLERLKKKGYEVLFMVEAIDEYAIGQLKEYEGKKLVSATKEGLKKGRVEVPI*

Protein Length: 538

Fln nts: CCTAATTTTTTCGCTACGAATCGATCTTTTCTCTACAAGGGTTTGAAGGATCGTTTCGGCGTTTTTCTCGTCTGTAAATTCTTCGCTCCGAATCCATCTTTTTGCTACAAGGGTTTCGTTTCGGCGTTTTTCTCGTCTGTAACTTCTTCGCTCCGAATCCATCTTTTCTCTACAAGGGTTTGAAGGCTCGTTTCGGTGTTTTTCTCGTGTTTTCGTTAAGCTTTCGAGGAGAAATC_-_ATGGCGGAAGCTGGTGAAGTTGAGACCTTTGCCTTCCAGGCTGAGATCAACCAGCTTTTGAGTCTTATTATCAACACTTTTTACAGCAACAAGGAAATTTTTCTTAGAGAGATCATCAGCAATTCCTCAGATGCTTTGGACAAGATCCGGTTTGAGAGTCTCACGGACAAGAGTAAGCTGGATGCACAACCAGAACTGTTTATTCACATTATTCCAGACAAGACAAACAATTCGTTGACTATTATCGACAGTGGTATTGGCATGACCAAATCTGATCTCGTGAACAACCTGGGAACCATTGCACGATCTGGCACGAAAGAGTTTATGGAAGCTCTTGCTGCTGGTGCTGATGTTAGCATGATTGGTCAGTTTGGTGTGGGTTTCTACTCTGCGTATTTGGTTGCTGAGAAGGTGCTTGTCACCACCAAACACAATGACGATGAGCAATATATTTGGGAATCGCAGGCCGGCGGATCGTTTACTGTTACCAGAGACACAAGTGGGGAGGTTCTTGGTAGAGGAACCAAAATCACACTGTTGCTCAAAGATGATCAGCTGGAATACCTCGAGGAGAGGCGTTTGAAGGACCTTACTAAGAAGAATTCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTCACATGAATGGAGTCTTGTGAACAAACAGAAGCCTATCTGGATGAGAAAACCAGAGGATATTACGAAGGAAGAATATGCGGCATTTTACAAGAGTCTGACAAATGACTGGGAAGAACACTTGGCAGTGAAACACTTCTCTGTTGAGGGACAGCTTGAGTTCAAGGCCGTCCTCTTTGTTCCCAAGAGGGCACCGTTTGATCTCGTCGATACCAAGAAGAAGTTAAATAACATCAAGCTCTATGTCAGGCGTGTATTCATCATGGACAACTGTGAAGAGTTGATCCCGGAATACCTTAGCTTTGTAAAGGGTGTCGTGGACTCTGAAGACCTTCCTTTGAATATTTCTCGCGAGACTCTCCAACAAAACAAAATCCTAAAAGTTATTAGGAAGAATCTTGTCAAGAAGTGTGTAGAGTTGTTCTTTGAAATTGCTGAAAACAAGGAGGATTACAATAAATTCTACGAGGCATTTTCAAAGAACCTTAAGCTTGGGATTCATGAGGATAGCCAGAACAGATCCAAGATTGCTGACTTGCTCAGGTACCACTCTACTAAGAGTGGAGATGAGATGACCAGCTTGAAGGATTATGTGACAAGAATGAAGGAGGGTCAAAAGGATATCTATTACATAACTGGTGAAAGCAAGAAGGCAGTTGAGAACTCTCCATTCTTGGAGCGTCTGAAGAAGAAGGGTTACGAAGTTCTTTTCATGGTTGAGGCTATTGATGAATATGCCATTGGGCAATTGAAGGAATACGAAGGCAAAAAATTGGTGTCTGCCACAAAGGAGGGGTTGAAAAAAGGAAGAGTTGAAGTCCCTATTTGATGGTCTCTGCAAAGTTATCAAGGATATTCTCGGGGACAAGGTGGAGAAAGTTGTCGTCTCTGACCGTGTTGTGGATTCTCCTTGCTGTTTGGTTACTGGAGAGTATGGATGGACTGCTAACATGGAAAGAATCATGAAGGCCCAGGCTTTGAGAGACAGTAGCATGTCAAGTTACATGTCAAGCAAGAAGACCATGGAAATTAATCCCGAGAATCCTATCATGGATGAGCTTAGAAAAATGACAAGTCTGTCAAGGATTTAGTTCTATTGATGTACGAAACTGCTTTGTTGACATCAGGGTTTAGCCTTGATGATCCTAATACTTTTGGTAACAGGATTCACAGGATGATGAAGCTGGGT

Fln Alignment:

Contig21674___ETFAFQAEINQLLSLIINTFYSNKEIFLREIISNSSDALDKIRFESLTDKSKLDA--QPELFIHIIPDKTNNSLTIIDSGIGMTKSDLVNNLGTIARSGTKEFMEALAAGADVSMIGQFGVGFYSAYLVAEKVLVTTKHNDDEQYIWESQAGGSFTVTRDTSGEVLGRGTKITLLLKDDQLEYLEERRLKDLTKKNSxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSHEWSLVNKQKPIWMRKPEDITKEEYAAFYKSLTNDWEEH--LAVKHFSVEGQLEFKAVLFVPKRAPFDLVDTKKKLN-NIKLYVRRVFIMDNCEELIPEYLSFVKGVVDSEDLPLNISRETLQQNKILKVIRKNLVKKCVELFFEIAENKED-----------------------YNKFYEAFSKNLKLGIHEDSQNRSKIADLLRYHSTKSGDEMTSLKDYVTRMKEGQKDIYYITGESKKAVENSPFLERLKKKGYEVLFMVEAIDEYAIGQLKEYEGKKLVSATKEGLKKGR
A7YAU9_______________QKFEFQAEVSRLMDILINSLYSNKDIFLRELISNASDALDKIRFMALTDKNVLGEGDNTRLDIKIKLDKENKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFLEQMQKGGDLNLIGQLGVGFYSVYLVADHVEVISKNNDDKQYIWESKADGAFAVSEDTENEPLGRGTEIRLHLKDEASEYLDETKLKDLVKKYSxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTYEWELLNDVKAVWLRNPKEVTDEEYAKFYHSLAKDFSEEKPLAWSHFTAEGDVEFKSILFIPPKAPHDLYESYCNAKANIKLYVRRVFISDEFEEFLPKYLGFLMGIVNSDTLPLNVSREMLQQHNSLNTIKKKLIRKALDMIRRIAEEDLDESDAKGKTDAESESEPDTEKKGKYVKFWNEFGKSIKLGIIEDASNRNHLAKLLRFESTKSGSKLASLDQYISRMKPGQKDIYYITGSSKEQLEKSPFLEKLKKKNYEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKIGK

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
651		94	5		0.998397
820	88		11		0.923564
828	88	11			0.923564
829		11		88	0.923564
831	87			12	0.992554
832	87			12	0.992554
836			88	11	0.923564
837	12	87			0.992554
838	87			12	0.992554
842	87			12	0.992554
846	12			87	0.992554
849	87			12	0.992554
850		14		85	0.993533
853	14		85		0.993533
855	14			85	0.993533
857	14			85	0.993533
858	14	85			0.993533
860	14			85	0.993533
900	16			83	0.994512
1218			33	66	0.997452
1220		66		33	0.997452
1222	66			33	0.997452
1223			33	66	0.997452
1227	66			33	0.997452
1228	66			33	0.997452
1233	66	33			0.997452
1234	66			33	0.997452
1238	66	33			0.997452
1240	66	33			0.997452
1242	66	33			0.997452
1244	33	66			0.997452
1245	66			33	0.997452
1388	50	50			1.0
1389		50		50	1.0
1401	75		25		0.996473
1514		33		66	0.997452
1536	66	33			0.997452
1555	25		75		0.996473
1601		25		75	0.996473
1602	25			75	0.996473
1603	75			25	0.996473
1610	66		33		0.997452
1800	66		33		0.997452

SSRs (tot: 2)

Start position	End position	Sequence	Length
913	926	GAG GAG GAG GAG GA	14
989	1004	AGA AGA AGA AGA AGA A	16

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain