SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene21657

Length: 1331 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene21657

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Flavonoid 3',5'-hydroxylase n=2 Tax=Cyclamen RepID=D0EUZ8_9ERIC	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Sma3	Flavonoid 3'-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Flavonoid 3'-monooxygenase.	EC:1.14.13.21	-	2.177e-10	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Flavonoid biosynthesis	00941		2.177e-10	%
Sma3	Flavone and flavonol biosynthesis	00944		2.177e-10	%
Sma3	Biosynthesis of phenylpropanoids	01061		2.177e-10	%
Sma3	Metabolic pathways	01100		2.177e-10	%
Sma3	Biosynthesis of secondary metabolites	01110		2.177e-10	%
Sma3	Flavonoid 3',5'-hydroxylase.	EC:1.14.13.88	-	1.466e-10	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	1.466e-10	%
Sma3	Flavone and flavonol biosynthesis	00944	1.466e-10	%
Sma3	Biosynthesis of secondary metabolites	01110	1.466e-10	%

Source	Gene names
Sma3	5'H; 5'h; At5g07990; CYP75; CYP75A1; CYP75A12\|F3'5'H1; CYP75A13; CYP75A2; CYP75A3; CYP75A4; CYP75A5; CYP75A6; CYP75A7; CYP75B1; CYP75B2; CYPEG1; CtF3'5'H; F13G24.190; F3'; F3'5'H; F3'H; F3'H1; F3'H2; F3'H3; F3H1; F3`H1; F3`H2; Fuchsia; GSVIVT00000646001;

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	flavonoid 3'-monooxygenase activity	GO:0016711	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	flavonoid 3',5'-hydroxylase activity	GO:0033772	Molecular Function	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G07990.1	TT7, CYP75B1, D501 Cytochrome P450 superfamily protein chr5:2560437-2562859 FORWARD LENGTH=513	46%
RefSeq	Arabidopsis thaliana	NP_196416.1	Flavonoid 3'-monooxygenase [Arabidopsis thaliana]	46%
RefSeq	Populus trichocarpa	XP_002314003.1	cytochrome P450 flavonoid 3',5'-hydroxylase [Populus trichocarpa]	66%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LN92

Fln msg: your sequence is shorter than subject: 406 - 452

Fln protein: NRPGNAGATYIAYDSQDMVWAAYGGRWKMERKVCNMHMLGGKALEDWQPVRDAEMGFMLRNILKHSQRGETVTVPDLLNICAANMIGQIILSKRVFEMEGDEANEFKDMVVELMTSAGYFNIGDFIPSVAWMDLQGIQRGMKKLHKKWDALIQRMIEEHQSTAKQRASNPDFLDVVMSQRDNCDGQGGRLSDVHIKALLLNLFTAGTDTSSSVIEWTLAEVINNPKLLKRVHQEMDTVIGRERRLKESDLANLPYFVAVCKEGFRKHPSTPLSLPRVSTEACEVDGYYIPKNTRLMVNIWGIGRDPEVWEKPEEFNPDRFVGSKIDPRGNDFELIPFGAGRRICAGTRMGITMVEYNLGSLIHAFGWDVPPNQEGLNMDEAFGLALQKAVPLVAKASPRLPLHLY*

Protein Length: 407

Fln nts: AACCGCCCGGGAAATGCAGGAGCCACGTACATCGCCTACGATTCCCAGGACATGGTGTGGGCAGCGTATGGAGGACGGTGGAAGATGGAGCGCAAGGTGTGCAACATGCACATGTTGGGAGGAAAGGCGTTGGAGGATTGGCAGCCGGTAAGAGACGCGGAGATGGGCTTCATGCTCCGCAACATTCTCAAACACTCGCAACGCGGCGAGACGGTAACCGTGCCGGACCTCCTCAACATCTGCGCCGCCAACATGATCGGGCAGATCATTCTGAGCAAACGGGTGTTCGAGATGGAAGGGGACGAGGCGAACGAGTTCAAGGACATGGTGGTGGAACTCATGACCTCCGCTGGATACTTCAATATCGGAGACTTCATTCCATCGGTGGCGTGGATGGACTTGCAGGGCATCCAGCGGGGTATGAAGAAGCTCCACAAGAAATGGGACGCACTCATACAGAGGATGATAGAGGAACACCAGTCCACGGCCAAGCAACGAGCGTCTAACCCCGATTTCCTAGACGTTGTGATGTCTCAGAGAGACAACTGCGACGGACAAGGCGGGAGGCTCTCCGATGTTCATATCAAAGCTCTACTGCTGAATTTATTCACAGCAGGAACCGACACGTCCAGCAGCGTGATTGAATGGACACTGGCGGAAGTAATCAACAACCCCAAGTTACTGAAGCGGGTGCATCAGGAGATGGACACGGTAATCGGGCGTGAGAGGAGACTGAAGGAGTCGGACCTTGCAAACCTGCCCTACTTTGTTGCCGTCTGCAAAGAGGGATTTCGGAAGCACCCGTCCACACCGCTCAGCCTCCCGCGCGTCTCCACCGAAGCGTGCGAGGTCGACGGTTACTACATTCCGAAGAACACTCGGCTAATGGTGAACATCTGGGGAATCGGGAGAGATCCGGAGGTGTGGGAGAAGCCGGAGGAGTTCAATCCTGACAGGTTTGTGGGTTCCAAGATCGATCCTCGTGGCAATGACTTCGAGCTCATCCCGTTCGGCGCAGGCAGGCGCATCTGTGCAGGTACTCGCATGGGGATTACGATGGTGGAGTACAATCTGGGGTCGTTGATCCATGCTTTCGGTTGGGATGTGCCACCAAACCAGGAGGGGCTCAATATGGACGAAGCATTCGGGCTGGCCCTGCAGAAGGCCGTGCCTCTCGTGGCCAAGGCCTCTCCTCGCCTTCCTCTTCATCTCTACTAA___TTACTGATATGCCTGCACCATCCAAAAGTTGTTAGTGTTAAAAATAAAGGCTGTTCCCCGTTACCGTGCACTTTACTTTCAAATTCCCTTGAAGCTGTATAATGTGCACTTAC

Fln Alignment:

Contig21657___NRPGNAGATYIAYDSQDMVWAAYGGRWKMERKVCNMHMLGGKALEDWQPVRDAEMGFMLRNILKHSQRGETVTVPDLLNICAANMIGQIILSKRVFEMEGDEANEFKDMVVELMTSAGYFNIGDFIPSVAWMDLQGIQRGMKKLHKKWDALIQRMIEEHQSTAKQRASNPDFLDVVMSQRDNCDGQGGRLSDVHIKALLLNLFTAGTDTSSSVIEWTLAEVINNPKLLKRVHQEMDTVIGRERRLKESDLANLPYFVAVCKEGFRKHPSTPLSLPRVSTEACEVDGYYIPKNTRLMVNIWGIGRDPEVWEKPEEFNPDRFVGSKIDPRGNDFELIPFGAGRRICAGTRMGITMVEYNLGSLIHAFGWDVPPNQEGLNMDEAFGLALQKAVPLVAKAS
B8LN92_______________NRPGNAGATYIAYDSQDMVWAAYGGRWKMERKVCNLHMLGGKALEDWQPVRDGEMGFMLRNILKHSQQGETVNVPDLLNICAANMIGQIILSKRVFETEGDEANEFKDMVVELMTSAGYFNIGDFIPSLAWMDLQGIQRGMKKLHKRWDALIARMIEEHQSTAKQRASRPDFLDIVMSQRDNCDGQGGRLSDVHIKALLLNLFTAGTDTSSSVIEWTLAELVNNPKLLKRVQHEMDTVIGRERRLKESDLANLPYFVAVCKEGFRKHPSTPLSLPRVSTEACEVNGHYIPKNTRLMVNIWGIGRDPEVWEKPEEFNPERFVGSKIDPRGNDFELIPFGAGRRICAGTRMGITMVEYNLGSLIHAFNWDLPPNQDGLNMDEAFGLALQKAVPLVAKAS

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
939			80	20	0.995493
940	80	20			0.995493
944		20	80		0.995493
947		80	20		0.995493
948	80		20		0.995493
949	80	20			0.995493
952		80	20		0.995493
954		20	80		0.995493
957	80	20			0.995493
958	20		60	20	0.99255
1099	18		81		0.999994
1118		90	10		0.90298
1151		20	80		0.999996
1191	12			87	0.992554

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain