SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene21262

Length: 1859 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene21262

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam00150, Cellulase, Cellulase (glycosyl hydrolase family 5)	-	-	4.0e-08	14%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	48%
Sma3	(1-4)-beta-mannan endohydrolase	-	-	1.063e-13	-

Source	ECs	Term	Type	e value	Identity
Sma3	Mannan endo-1,4-beta-mannosidase.	EC:3.2.1.78	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Fructose and mannose metabolism	00051		0.0	%

Source	Gene names
Sma3	At1g02310; At2g20680; At3g10890; At3g10900; At3g30540; At4g28320; At5g01930; At5g66460; F26K10.200; F5H14.35; GSVIVT00010891001; GSVIVT00014580001; GSVIVT00018590001; GSVIVT00024894001; GSVIVT00032044001; GSVIVT00032045001; GSVIVT00032046001; GSVIVT000320

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	hydrolase activity	GO:0016787	Molecular Function	-
Sma3	mannan endo-1,4-beta-mannosidase activity	GO:0016985	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Bulb-type lectin domain	IPR001480	-	-
Sma3	Glycoside hydrolase, family 5	IPR001547	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Glycoside hydrolase, family 5, conserved site	IPR018087	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G66460.1	MAN7, AtMAN7 Glycosyl hydrolase superfamily protein chr5:26538911-26540837 REVERSE LENGTH=431	56%
RefSeq	Arabidopsis thaliana	NP_201447.1	mannan endo-1,4-beta-mannosidase 7 [Arabidopsis thaliana]	56%
RefSeq	Populus trichocarpa	XP_002306881.1	predicted protein [Populus trichocarpa]	62%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9Z9

Fln msg: your sequence is shorter than subject: 412 - 429

Fln protein: MGGGMLMRFGAFACLFFLSFLQQAAARRNGFVKTKGTHFSVNGNPLFVNGFNSYWLMTMAADPTQRNKVTSAFEQAAASRLNVARTWAFNDGQYEALQTSPGVYDEHVFQALDFVISEAKKYGLRLILSLANNYADFGGKAQYVQWARNAGQNLNSEDDFFTNPTVKGYYKNHIKTVLTRVNSITGVAYKDDPTIFSWELMNEPRCPSDPSGRTIQGWIEEMASHVKSIDRNHLLEAGLEGFYGESIKENNPGFYVGTNFITNNQIKGIDFATIHCYPDNWLSGSDEEAQLSFVKSWIESHLKYGKRILKKPVLLAEFGKSSKDPGFDISQRNELFVVIYKHMYASARRGGASGGAMFWQLMAEGMESSGDGYEIVLSQNPSTTSIISAQSHKLAELTHLLTKLYETGGRD*

Protein Length: 413

Fln nts: GGAGCTCTCCAATACAGAGAGCCTCCTTCGAGTAGTTTTAACTCATAAACTTGGGAATCTGCATTGTTTTCTGAAGTCTGTGGAGTTATTTTTCTCAA_-_ATGGGTGGTGGTATGCTGATGCGTTTTGGGGCTTTTGCCTGTCTGTTCTTTCTAAGTTTTCTTCAGCAAGCTGCAGCCAGAAGAAATGGGTTTGTGAAGACAAAGGGCACCCATTTTTCTGTCAATGGGAATCCACTGTTTGTCAATGGGTTCAACTCTTACTGGTTAATGACTATGGCAGCAGATCCAACACAGAGAAATAAGGTTACCTCTGCTTTTGAACAAGCTGCAGCTTCTCGGCTCAATGTTGCCAGAACTTGGGCATTCAACGATGGCCAGTACGAGGCATTGCAGACTTCTCCCGGTGTATATGATGAACATGTCTTTCAGGCCCTGGACTTCGTGATCTCTGAGGCCAAGAAGTATGGTCTCAGACTAATACTTAGCCTGGCCAACAATTATGCGGACTTTGGAGGAAAGGCCCAATATGTACAATGGGCTCGCAACGCTGGACAGAATCTCAACTCTGAAGATGATTTTTTCACCAATCCCACTGTTAAAGGATACTATAAGAACCATATTAAGACTGTATTGACCAGAGTGAACAGCATTACTGGAGTAGCCTACAAAGATGACCCTACAATCTTTTCTTGGGAGCTCATGAACGAGCCTCGTTGTCCAAGTGATCCTTCTGGGAGGACCATCCAGGGATGGATAGAGGAGATGGCTAGCCATGTCAAATCCATTGATAGAAACCATCTTCTCGAGGCTGGGTTGGAAGGGTTTTATGGGGAATCCATAAAAGAAAACAACCCAGGCTTCTATGTGGGAACAAATTTCATTACCAATAACCAAATCAAAGGAATTGATTTTGCAACTATTCATTGCTATCCTGATAATTGGCTTTCTGGATCAGATGAAGAGGCCCAACTTTCGTTTGTGAAGAGTTGGATAGAGAGTCATTTGAAATATGGGAAAAGAATACTGAAGAAACCAGTTCTGTTAGCAGAGTTTGGGAAATCCTCTAAAGACCCGGGATTCGACATTAGCCAGAGAAACGAGTTATTTGTTGTGATTTATAAACACATGTATGCTTCTGCTAGGAGGGGAGGAGCGAGTGGAGGAGCCATGTTTTGGCAGCTGATGGCAGAAGGAATGGAGTCATCTGGAGATGGGTATGAGATTGTTTTGTCTCAGAACCCATCCACAACCTCTATTATTTCTGCTCAGTCTCATAAATTGGCTGAGCTTACTCATTTGTTAACAAAGTTGTATGAGACTGGAGGCCGAGATTGA___TTGTCCAATTTGTGTACATATTTGGTGAGAGTATTTCTGCATAAGTAGTAGTTATTATTGAAATCTATAGTGATCAGGTATTAGTAAAATCTCGATTGAGCAGCTTGATGTTGATGATAGTCAAAAGCCTCTGAACCAGAGCGAGAAGATGGGGAGCTGCGTAAACATCGAGTTCCAGTTCCATAGGTGCCCTTGGCTAAAAGTGAAGAAAACAAGCAGGCGCAAATTGGTATATACATGAATTTTACTATGAGGAGATGCAAGAGTATATGCTTGTTACTCTATAAACCAATGTTGCATATTTGATTAGACACATCCTATATGCATAATATTTGAAGAAAGTTATCTAGGATTATGAGGGTTAGTACTCTCTCACTTTTGTAATGGGTGAGGTTGTAAGTTTACATCCTCTTTGATTTTATGTAAGGGAGAGTATCTCCTATATGATTGTATTATTAATATTTCAATGCCATAGGCAGGGTCATTGAATTTATAATTATAAAATTTAGTTTAATTTACATATTT

Fln Alignment:

Contig21262___GAFACLFFLSF------LQQAAARRNGFVKTKGTHFSVNGNPLFVNGFNSYWLMTMAADPTQRNKVTSAFEQAAASRLNVARTWAFNDGQYEALQTSPGVYDEHVFQALDFVISEAKKYGLRLILSLANNYADFGGKAQYVQWARNAGQNL-NSEDDFFTNPTVKGYYKNHIKTVLTRVNSITGVAYKDDPTIFSWELMNEPRCPSDPSGRTIQGWIEEMASHVKSIDRNHLLEAGLEGFYGESIK---ENNPGFY---VGTNFITNNQIKGIDFATIHCYPDNWLSGSDEEAQLSFVKSWIESHLKYGKRILKKPVLLAEFGKSSKDPGFDISQRNELFVVIYKHMYXXXXXXXXXXXXMFWQLMAEGMESSGDGYEIVLSQNPSTTSIISAQSHKLAEL
D5A9Z9_______________GVVSCIVFLYLNFGDVHILPFSQPRMSFVERNGSHFVVDDKELYVNGWNSYWLMDQSVGDSTRHRVKTMLRRGAEMGLTVVRTWAFNDGGYNALQQFPGTFDERVLRALDFVIVEARRNGLRLIFSLVNNLNAYGGKVQYVEWAHAAGINVGSSNDSFFSDPTIRGYYKEYVKSILVRKNSISGVEYRNEPAIFAWELVNEPRCTSDASGQTLQTWISEMAKFIKSLDSRHLLTVGLEGFYGKTTPNKIELNPGDWAVSLGSDFIWNSQVEEIDFASVHAYPDSWIPNVDLEDHLNYFSKWVTSHIDDGEQILKKPVLFTEFGLSSHHKGFEQSHRDRLFKTMYEEIDESARKGGAGGGALIWQFVVEGMEEYGDEFAFVPWQFPSTHKLIVEQSCRLQTL

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
1681	12	87			0.992554
1682		12		87	0.992554
1684			87	12	0.992554
1685			87	12	0.992554
1696	14			85	0.993533
1697		14		85	0.993533
1699			85	14	0.993533
1723		16		83	0.994512
1773		60		40	1.0
1780	25		75		1.0
1789	85			14	0.993533
1792	85			14	0.993533
1793	83	16			0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain