SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene19099

Length: 1966 nt

UniGene Fasta

>sp_v3.0_unigene19099
ATGGGGGCATACCACTGCATAAACAGTGCAACCAACGCATGAGGACATGGGGGATCTGGGCAGACAGGAACGCCCATCCTTTTCATTTTGAGGGTGACTGCTTTGCTTGGTCCATCCATGTGGCTTTGAATCTTTTGAGAGACTGGTTTGGTTTTCAGCGAGGGTTACGCCCAGACCGGTGGAGAGAAATTCCCTATTCGTGCCCAAATCAGTCAAAAACTAAGATTGTATCAGTATGGGAATCCTTCTGCTGTTTGCTCTGCTGGCATTGTTTGCCATCGCAGGCAGCGCTTCCAGAGCAGATTTTTCCATCATCGGGTATGATAGCAAAGACCTGAGGGAAGATGATGCGATCTCGGAGCTCTATGAACTGTGGCTCGCTCAGCACAGGAAAGCCTATAATGGCCTTGACGAGAAACAGAAGAGGTTCTCTGTATTTAAAGACAATTTTCTGTATATTCATCAGCACAACAACCAAGAGAATCCATCCTTCAAACTGGGGCTGAACCAGTTTGCAGATCTAAGTCACGAGGAATTCAAGGCCACATATTTGGGTGCCAAGCTGGATACCAAGAAACGCTTGTCCAAGTCTCCCAGCCCTCGATACCAGTATTCGGAGGGCGAGAATTTGCCAGAGTCCGTTGACTGGAGAGAAAAGGGAGCCGTGGCTGCTGTTAAGGACCAGGGCTCCTGCGGAAGTTGTTGGGCGTTCTCGACTGTGGCTGCTGTTGAAGGCATCAATCAAATTGTGACTGGCAATTTGACTTCCCTGTCCGAGCAGGAACTGGTGGACTGCGATACTTCTTACAACCAAGGATGCAATGGCGGTCTCATGGATTATGCTTTCCAGTTTATCATAGACAACGGTGGGCTTGACAGTGAGGATGATTACCCTTACACGGCCAACGCTGGCAGCTGTGACGCTTACCGAAAAAATGCCCATGTGGTGACAATTGATAGCTACGAAGATGTGCCTGAGAACGATGAGAAGTCGCTGAAGAAGGCTGCGGCGCATCAGCCGATTAGCGTTGCCATCGAAGCCAGCGGAAGGGCGTTCCAGTTTTACGAATCTGGCGTATTCACAAGCACCTGCGGAACTCAGCTGGACCACGGTGTCACTCTGGTAGGGTATGGTTCAGAGTCCGGCAAAGATTATTGGATTGTGAAGAATTCTTGGGGAAAGAGCTGGGGAGAGAAGGGTTTCATCAGACTGCAGAGGAACATTGAAGGGGCTTCGACGGGAATGTGCGGTATAGCGATGGAGGCATCGTACCCTGTAAAGAAGGGTTCCAACCCTCCAAACCCTGGGCCTTCTCCCCCATCCCCTGTGAAGCCTCCTACCGTGTGTGATAACTACTATTCCTGCCCGGAGAGCAACACCTGCTGTTGCATGTATGACTTTGGGGGTTACTGCTATGCATGGGGTTGCTGCCCTCTCAACTCTGCCACATGCTGCGACGATCACTACAGTTGCTGCCCTAGCGATCATCCGGTTTGCGATCTCGATGCACAGACATGCCTCAAGAGTCGCAAGGATCCATTTGGAACGAAGATGCTGAAGCGTACCCCGGCCAAACCTTACTGGGCTCTCTCTGGTCAGAAAGCGGTCACTGAAAGGAGCTGATTTCTCCCTTCAATCCCAGCCCTCTAAACTCAACAACAGGCTGGTCCGAATGGGGGAAGAAATTAAATTTTAATGCATGAAAAAATTTCAAACATATTTATCGATAAATATTCCTGCACATATGGCTTCTCCAAATCGGCCAAGACACTTCCCATCTCTGTTCTCTCGTCAATTCCATTTCTATTTCTATTTGTGTATGTACAGATCTTTCTATGGACGACCATTATCAAAGCTGAAGCCTGTATCAGCGCAAAACGGCTTTATTACAGAACAGCTTTGTGATTCTCAAAAGCCGTATCAAGCTGATACAGGCTCAGCTTTGATAATGGTCGTCCATAGAAA

Ace file of the UniGene sp_v3.0_unigene19099

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pseudotzain n=3 Tax=Pinaceae RepID=Q40922_PSEMZ	-	-	88%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Sma3	Putative uncharacterized protein	-	-	-

Source	GOs	Term	Type	e value	Identity
Sma3	cysteine-type endopeptidase activity	GO:0004197	Molecular Function	0.0	-
Sma3	proteolysis	GO:0006508	Biological Process	0.0	-

Source	InterPros	Term	Type	Identity
Sma3	Granulin	IPR000118	-	-
Sma3	Peptidase, cysteine peptidase active site	IPR000169	-	-
Sma3	Peptidase C1A, papain	IPR013128	-	-
Sma3	Peptidase C1A, papain C-terminal	IPR000668	-	-
Sma3	Proteinase inhibitor I29, cathepsin propeptide	IPR013201	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G47128.1	RD21, RD21A Granulin repeat cysteine protease family protein chr1:17283139-17285609 REVERSE LENGTH=462	63%
RefSeq	Arabidopsis thaliana	NP_564497.1	cysteine proteinase RD21a [Arabidopsis thaliana]	63%
RefSeq	Populus trichocarpa	XP_002326950.1	predicted protein [Populus trichocarpa]	67%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUC2

Fln msg:

Fln protein: MGILLLFALLALFAIAGSASRADFSIIGYDSKDLREDDAISELYELWLAQHRKAYNGLDEKQKRFSVFKDNFLYIHQHNNQENPSFKLGLNQFADLSHEEFKATYLGAKLDTKKRLSKSPSPRYQYSEGENLPESVDWREKGAVAAVKDQGSCGSCWAFSTVAAVEGINQIVTGNLTSLSEQELVDCDTSYNQGCNGGLMDYAFQFIIDNGGLDSEDDYPYTANAGSCDAYRKNAHVVTIDSYEDVPENDEKSLKKAAAHQPISVAIEASGRAFQFYESGVFTSTCGTQLDHGVTLVGYGSESGKDYWIVKNSWGKSWGEKGFIRLQRNIEGASTGMCGIAMEASYPVKKGSNPPNPGPSPPSPVKPPTVCDNYYSCPESNTCCCMYDFGGYCYAWGCCPLNSATCCDDHYSCCPSDHPVCDLDAQTCLKSRKDPFGTKMLKRTPAKPYWALSGQKAVTERS*

Protein Length: 464

Fln nts: ATGGGGGCATACCACTGCATAAACAGTGCAACCAACGCATGAGGACATGGGGGATCTGGGCAGACAGGAACGCCCATCCTTTTCATTTTGAGGGTGACTGCTTTGCTTGGTCCATCCATGTGGCTTTGAATCTTTTGAGAGACTGGTTTGGTTTTCAGCGAGGGTTACGCCCAGACCGGTGGAGAGAAATTCCCTATTCGTGCCCAAATCAGTCAAAAACTAAGATTGTATCAGT_-_ATGGGAATCCTTCTGCTGTTTGCTCTGCTGGCATTGTTTGCCATCGCAGGCAGCGCTTCCAGAGCAGATTTTTCCATCATCGGGTATGATAGCAAAGACCTGAGGGAAGATGATGCGATCTCGGAGCTCTATGAACTGTGGCTCGCTCAGCACAGGAAAGCCTATAATGGCCTTGACGAGAAACAGAAGAGGTTCTCTGTATTTAAAGACAATTTTCTGTATATTCATCAGCACAACAACCAAGAGAATCCATCCTTCAAACTGGGGCTGAACCAGTTTGCAGATCTAAGTCACGAGGAATTCAAGGCCACATATTTGGGTGCCAAGCTGGATACCAAGAAACGCTTGTCCAAGTCTCCCAGCCCTCGATACCAGTATTCGGAGGGCGAGAATTTGCCAGAGTCCGTTGACTGGAGAGAAAAGGGAGCCGTGGCTGCTGTTAAGGACCAGGGCTCCTGCGGAAGTTGTTGGGCGTTCTCGACTGTGGCTGCTGTTGAAGGCATCAATCAAATTGTGACTGGCAATTTGACTTCCCTGTCCGAGCAGGAACTGGTGGACTGCGATACTTCTTACAACCAAGGATGCAATGGCGGTCTCATGGATTATGCTTTCCAGTTTATCATAGACAACGGTGGGCTTGACAGTGAGGATGATTACCCTTACACGGCCAACGCTGGCAGCTGTGACGCTTACCGAAAAAATGCCCATGTGGTGACAATTGATAGCTACGAAGATGTGCCTGAGAACGATGAGAAGTCGCTGAAGAAGGCTGCGGCGCATCAGCCGATTAGCGTTGCCATCGAAGCCAGCGGAAGGGCGTTCCAGTTTTACGAATCTGGCGTATTCACAAGCACCTGCGGAACTCAGCTGGACCACGGTGTCACTCTGGTAGGGTATGGTTCAGAGTCCGGCAAAGATTATTGGATTGTGAAGAATTCTTGGGGAAAGAGCTGGGGAGAGAAGGGTTTCATCAGACTGCAGAGGAACATTGAAGGGGCTTCGACGGGAATGTGCGGTATAGCGATGGAGGCATCGTACCCTGTAAAGAAGGGTTCCAACCCTCCAAACCCTGGGCCTTCTCCCCCATCCCCTGTGAAGCCTCCTACCGTGTGTGATAACTACTATTCCTGCCCGGAGAGCAACACCTGCTGTTGCATGTATGACTTTGGGGGTTACTGCTATGCATGGGGTTGCTGCCCTCTCAACTCTGCCACATGCTGCGACGATCACTACAGTTGCTGCCCTAGCGATCATCCGGTTTGCGATCTCGATGCACAGACATGCCTCAAGAGTCGCAAGGATCCATTTGGAACGAAGATGCTGAAGCGTACCCCGGCCAAACCTTACTGGGCTCTCTCTGGTCAGAAAGCGGTCACTGAAAGGAGCTGA___TTTCTCCCTTCAATCCCAGCCCTCTAAACTCAACAACAGGCTGGTCCGAATGGGGGAAGAAATTAAATTTTAATGCATGAAAAAATTTCAAACATATTTATCGATAAATATTCCTGCACATATGGCTTCTCCAAATCGGCCAAGACACTTCCCATCTCTGTTCTCTCGTCAATTCCATTTCTATTTCTATTTGTGTATGTACAGATCTTTCTATGGACGACCATTATCAAAGCTGAAGCCTGTATCAGCGCAAAACGGCTTTATTACAGAACAGCTTTGTGATTCTCAAAAGCCGTATCAAGCTGATACAGGCTCAGCTTTGATAATGGTCGTCCATAGAAA

Fln Alignment:

Contig19099___RADFSIIGYDSKDLREDDAISELYELWLAQHRKAYNGLDEKQKRFSVFKDNFLYIHQHNNQENPSFKLGLNQFADLSHEEFKATYLGAKLDTKKRLSKSPSPRYQYSEGENLPESVDWREKGAVAAVKDQGSCGSCWAFSTVAAVEGINQIVTGNLTSLSEQELVDCDTSYNQGCNGGLMDYAFQFIIDNGGLDSEDDYPYTANAGSCDAYRKNAHVVTIDSYEDVPENDEKSLKKAAAHQPISVAIEASGRAFQFYESGVFTSTCGTQLDHGVTLVGYGSESGKDYWIVKNSWGKSWGEKGFIRLQRNIEGASTGMCGIAMEASYXXXXXXXXXXXXXXXXXXXXXXTVCDNYYSCPESNTCCCMYDFGGYCYAWGCCPLNSATCCDDHYSCCPSDHPVCDLDAQTCLKSRKDPFGTKMLKRTPAKPYWALSGQKAVTERS
A9NUC2_______________RADFSIIGYDSKDLREDDAIMELYELWLAQHKKAYNGLGEKQNRFSVFKDNFLYIHQHNNQGNPSYKLGLNQFADLSHEEFKATYLGAKLDTKKRLSNSPSPRYQYSDGEDLPESIDWREKGAVTAVKDQGSCGSCWAFSTVAAVEGINQIVTGNLTSLSEQELVDCDTSYNQGCNGGLMDYAFQFIINNGGLDSEDDYPYKANDGSCDAYRKNAHVVTIDDYEDVPENDEKSLKKAAANQPISVAIEASGRAFQFYESGVFTSTCGTQLDHGVTLVGYGSESGTDYWIVKNSWGKSWGEKGFIRLQRNIEGVSTGMCGIAMEASYPLKKGANPPNPGPSPPSPVKPPTVCDNYYSCPESNTCCCMYDFGGYCYAWGCCPLNSATCCDDHYSCCPNDHPVCDLDAQTCLKSRKDPIGTKMLKRTPAKPYWALSGQEAVTERT

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
144	33		66		1.0
145		66	33		0.997452
146			33	66	0.997452
148			66	33	0.997452
201		20		80	0.995493
254	20			80	0.995493
705	16			83	0.994512
885	50		50		1.0
1042	25		75		0.996473
1097	14		85		0.993533
1200			88	11	0.923564
1201			88	11	0.923564
1379	95		4		0.995055
1571		97		2	0.946738
1646		97		2	0.97849
1862	80	1			1.0
1887			7	92	0.999999
1918			12	87	0.992554
1919		12		87	0.992554
1920		12	87		0.992554
1921			12	87	0.992554
1923	87			12	0.992554
1925		87		12	0.992554
1926	85		14		0.993533
1927	85	14			0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain