SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene18492

Length: 1283 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene18492

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	PREDICTED: similar to Bifunctional nitrilase/nitrile hydratase NIT4B n=1 Tax=Vitis vinifera RepID=UPI0001985AF6	-	-	0.0	78%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	90%
Sma3	Nitrilase 4	-	-	1.29e-22	-

Source	ECs	Term	Type	e value	Identity
Sma3	Nitrilase.	EC:3.5.5.1	-	2.624e-39	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Tryptophan metabolism	00380		2.624e-39	%
Sma3	Cyanoamino acid metabolism	00460		2.624e-39	%
Sma3	Aminobenzoate degradation	00627		2.624e-39	%
Sma3	Styrene degradation	00643		2.624e-39	%
Sma3	Nitrogen metabolism	00910		2.624e-39	%
Sma3	Metabolic pathways	01100		2.624e-39	%
Sma3	Cyanoalanine nitrilase.	EC:3.5.5.4	-	1.74e-11	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Cyanoamino acid metabolism	00460		1.74e-11	%

Source	Gene names
Sma3	AT3G44310; At3g44300; At3g44310; At3g44320; At5g22300; BrNIT1; BrNIT2; BrNIT3; BrNIT4; GSVIVT00003612001; GSVIVT00003674001; GSVIVT00003679001; GSVIVT00003682001; GSVIVT00003683001; GSVIVT00003685001; GSVIVT00003689001; GSVIVT00003691001; GSVIVT0000369300

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	nitrilase activity	GO:0000257	Molecular Function	-
Sma3	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	GO:0016810	Molecular Function	-
Sma3	nitrile hydratase activity	GO:0018822	Molecular Function	-
Sma3	cyanoalanine nitrilase activity	GO:0047427	Molecular Function	-
Sma3	3-cyanoalanine hydratase activity	GO:0047558	Molecular Function	-
Sma3	nitrogen compound metabolic process	GO:0006807	Biological Process	-
Sma3	indoleacetic acid biosynthetic process	GO:0009684	Biological Process	-
Sma3	cellular response to sulfate starvation	GO:0009970	Biological Process	-
Sma3	cyanide metabolic process	GO:0019499	Biological Process	-
Sma3	glucosinolate catabolic process	GO:0019762	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	detoxification of nitrogen compound	GO:0051410	Biological Process	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Nitrilase/cyanide hydratase, conserved site	IPR000132	-	0.0	-
Sma3	Nitrilase/cyanide hydratase	IPR003010	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G22300.1	NIT4, AtNIT4 nitrilase 4 chr5:7379401-7381764 FORWARD LENGTH=355	76%
RefSeq	Arabidopsis thaliana	NP_197622.1	bifunctional nitrilase/nitrile hydratase NIT4 [Arabidopsis thaliana]	76%
RefSeq	Populus trichocarpa	XP_002328237.1	nitrilase 1 [Populus trichocarpa]	75%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LLB3

Fln msg: Overlapping hits, possible frame ERROR between 681 and 668, atg_distance in limit (1-15): atg_distance = 14, W2: There is no M at the beginning, your sequence is shorter than subject: 330 - 346

Fln protein: GSASPNARTLRATVVQASTVFYDTPATLDKAERLIAEGAAYGSQLLVFPEAFIGGYPRGSNFGIVIGNRTFKGREEFRKYHASAIDVPGPEVERISAAAAKYKVHVIMGVIERAGFTLYCTVLFFDSQGRFLGKHRKLMPTSLERVIWGFGDGSTLPVYDTAIGRLGAVLCWENRMPLMRTAMYAKGVELYCAPTADARESWQISMRHIGLEGGCFVLSANQxxxxxxDLPPPPEYVFGGSEENMSPESVVCAGGSVIISPSGTVLAGPNFEGEGLITADLDLGEIARAKFDFDVVGHYSRPDVLKLMVNESPNNPATFSSGTIHSDAA*

Protein Length: 331

Fln nts: GGCTCGGCATCGCCCAACGCCAGGACTCTTCGAGCCACTGTTGTTCAGGCCTCTACTGTCTTCTACGACACCCCTGCTACTCTCGATAAAGCAGAAAGATTGATAGCAGAAGGTGCTGCTTATGGGTCACAGTTGCTTGTGTTTCCTGAAGCTTTTATTGGTGGCTATCCTCGAGGTTCTAATTTTGGTATTGTAATTGGAAATCGCACTTTTAAGGGTCGGGAGGAGTTCCGTAAATACCATGCTTCTGCTATTGATGTGCCAGGTCCAGAAGTAGAGAGGATATCAGCTGCAGCTGCAAAATATAAAGTGCATGTAATAATGGGTGTGATAGAGCGAGCAGGCTTCACACTGTATTGCACTGTTCTTTTCTTTGATTCTCAAGGAAGATTCCTAGGGAAGCACCGTAAACTGATGCCAACATCTTTGGAGCGTGTGATTTGGGGTTTTGGTGATGGATCCACCTTGCCTGTGTATGACACTGCAATTGGGCGTCTGGGTGCAGTCCTATGCTGGGAAAACCGAATGCCTCTTATGAGAACAGCTATGTATGCTAAAGGGGTGGAACTTTATTGTGCACCAACTGCTGATGCAAGAGAATCATGGCAAATATCAATGCGTCATATAGGTCTGGAGGGTGGATGTTTTGTGCTGTCAGCTAATCAGxxxxxxxxxxxxxxxxxxGACTTACCTCCTCCACCAGAGTATGTCTTTGGAGGTTCAGAGGAGAATATGTCTCCGGAGAGTGTTGTATGTGCAGGTGGAAGTGTTATCATTTCTCCTTCTGGTACTGTATTGGCGGGGCCCAATTTTGAAGGGGAGGGTCTAATTACTGCAGATCTCGATTTGGGTGAAATTGCACGAGCAAAATTTGATTTTGATGTTGTTGGGCACTATTCAAGACCTGATGTGTTGAAATTGATGGTGAATGAATCTCCAAATAATCCTGCCACATTTTCTTCAGGGACCATACACTCAGATGCTGCCTGAATGGATTTCAGAATTGCCATAATAGTCAACTATTCTACCATATGGCATGATGGACAAGCTTGAGCATGACTAGAAGGACCAATTTGTTATCAAGAACCGTTATTTAAGTACCTAAAATATAGAACTACAGAGTGTATTTTCTGTAAAATTTATCTTTGATGACCGTAAAGAACAATTTGCTATTGAGAAATCAAGGAAACATATATTTATAGTATTCAAAATATAGAACTACAGACACCGTAGCTTTTGTAGTAATTTATATTTGCATATAGCAAAAGTTAGACATTATTTTATC

Fln Alignment:

Contig18492___ASPNARTLRATVVQASTVFYDTPATLDKAERLIAEGAAYGSQLLVFPEAFIGGYPRGSNFGIVIGNRTFKGREEFRKYHASAIDVPGPEVERISAAAAKYKVHVIMGVIERAGFTLYCTVLFFDSQGRFLGKHRKLMPTSLERVIWGFGDGSTLPVYDTAIGRLGAVLCWENRMPLMRTAMYAKGVELYCAPTADARESWQISMRHIGLEGGCFVLSANQxxxxxDLPPPPEYVFGGSEENMSPESVVCAGGSVIISPSGTVLAGPNFEGEGLITADLDLGEIARAKFDFDVVGHYSRPDVLKLMVNESPNNPATFSSGT
B8LLB3_______________ASPNARTLRATVVQASTVFYDTPATVDKAERLIAEGAAYGSQLLVFPEGFIGGYPRGSNFGAVVGNRSFKGREEFRKYYASAIDVPGPEVERIAAAAAKYKVHVIMGVIERAGFTLYCTALFFDSQGRFLGKHRKMMPTALERVIWGFGDGSTLPVYDTTIGRLGALICWENRMPLLRTALYAKGVELYCAPTADARESWQASMLHIAMEGGCFVLSANQxxxxxDYPPPPDYLFGGPDENISPESVVSAGGSVIISPSGTVLAGPNFEGEALITADLDFGEIVRAKLDFDVVGHYARPDVLKLTVNDHPLNPVTFSSGT

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
700	66			33	1.0
704	66			33	1.0
712	33		66		1.0
982			84	15	0.999986
1017	66		33		1.0
1027		40		60	1.0
1157		42		57	1.0
1205			75	25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain