SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene18175

Length: 2322 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene18175
AGTTTGGAATCACACAGTCAGTTTGAAATCCTTCTTCCTGTCATTTTTGCTTTCCAGTAAAGTTATAACATTGGGTGCAAGTGGTTAAGAAAAGTAAGTAGGCTGATAAGATGGTTGGAATGTCACTGGAAATCCCACGAAACAGAAGTTGCCTCCCTCGGCTCAGCTTATAGGCTTTAATTTTGTCAATTTTGATGCCCTGACAATCAAGGCCAACCCATGAATGTGGCTCTGGATAGGAAATTTGAGGTGGATTGAAAGCTTATGCCTTGAGGCGCAAGGAAATTGTATCTTACTGGATAAATTAAGATGGGATGCCGAAGTTCTGCACCAGATCTCATGAGGTTGAATGAATGTGATGGTGTGTTTAACCAGATCTCATGAGGTTGAATGAATATCATGGTGTGTTTATGCATCCAAACACCTGGGAATGAAATTCCCCCTATCCTGTTTGTCACCTGAGTCTCGAAAGCTGCGAATGCATAAACTTTGTGTGAGATTTGGCTAGTTAAAAATGAGGTTTTTTCATGAATTGGGTGTGTGTGTCTTGGCGATTGTATTATTCTGTTGCTTAAGGACCTGTAATGGGCAGTTTGTCTCTCTTGTTGTAAATGCAAGTGCCAAGATTGCTGAGACGGATGAGAATTTCATATGTGCTACTTTAGATTGGTGGCCTCCTGAAAAATGTGATTATGGCAGATGCAGTTGGGTTGGTGCGTCCCTTCTGAATTTGGATCTCGAAAACCCAGTTTTTGCAAACGCCTTAAAAGCGTTCTCACCACTGAAAATCAGGTTGGGTGGAACTTTGCAAGACAAACTGATATATGACGTTGGACCTCTGTCACAACCCTGTCATCCTTTTGCTAGGGATGCTTCTAAGATGTTTGGGTTCTCAAAAGGTTGTCTTACCATGGACAGATGGGACAAGCTGAACAAGTTTGCCGCAAAAACAGGGTCTATGGTGGCATTTGGCTTAAATGCATTGTATGGTCGGTATCAAATATCTGCGGATGGTCTTTGGGGAGGTCCTTGGAATTCAAGCAATGCTCGCAGCTTTATTCAGTACACCGTCGACCATGGTTATAAGATATATGCATGGGAGTTTGGAAATGAGTTGAGTGGAGGTGGTATTGGTGCAAAAGTTAGTGCTATACAATATGCTGCTGATGTAACTGAGCTTAGTAAGATTTTGAAAGACATATACAAGCATTCTCCTGAGAAACCCTTAATGGTAGCACCAGATGGATTCTTTGATGCGACCTGGTTTCAAGAGCTTCTTCAACAGACTGGGCCAAATGTTCTGAATGTAGTTACACGACATATATACAATCTTGGAGCAGGTGTGGATAAGCATTTGATGCAGAAGATACTTAATCCAGGGTACTTAAGTCGAGAAGCTACAAAGTTCAAACAATTGCAAGCTATTTTGCAGAAATACGGTCCTTGGGCAAGCTCCTGGGTCGGAGAAGCGGGTGGAGCTTATAACGGTGGAAAGCATCGTGTCTCAGATGCTTTTGTGAATAGTTTTTGGTATTTAGATCAGCGTAGAATCATCTACATATGATACTAAAGTTTATTGTAGACAAAGCTTGATTGGTGGTAACTATGGCCTTCTTAACACAACAAATTTTGCACCCAATCCTGATTATTATAGTGCACTGCTTTGGCATCGGTTGATGGGGAGAGGTGTGCTTTCCATCAATATTACAGGGCCACCCTATGTGCGCACATATGCTCATTGTGCAAAGAATGATACTGGAATAACTTTACTCTTGATCAACCTCAGCAACGACACAAAAGTTGATATTCTTGTTTCTGCTTATCCAAATTCTACCATGTACAAGAGAAGACCGAATGCAAAAGAGTCTTTTATACATAAGATTAAGAGATTCGTAGCCTGGGCTGGAATCAAAACATCAAAGGCGAAAGATTATAGAATGGAATACCACTTAACACCAAAAGATGGTGACCTTCATAGTCAAACAGTGCTTTTAAATGGTGCAGAGTTGGAGTTAACTGAAGATGGGGATATTCCTTCGTTAGATCCTGTCATGGTTGATTCTGGGCTACCTATTCCAGTAGCTCCTTCATCTATTGCTTTTGTTTCCTTGCCTGGTCTAGAGATCTCTGCTTGTTTACAGTAGCAATGGTGTAGATATTTATTTGAAACATCAATAGAGCCTTCTAAATTGGGCTATTTATTGAGGTGTATTTGACCAAGTTACCAACAAATCAAGAAAATGTGTTGCTGATGCATGTTGCCACATTGTTTGTCTCAGGGCACGGATTATATAAATTTGTCCAAAGTTTCGAAGAGTCTTT

Ace file of the UniGene sp_v3.0_unigene18175 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Heparanase-like protein 2 n=4 Tax=Andropogoneae RepID=B6SRR5_MAIZE	-	-	0.0	57%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	65%
Sma3	Heparanase-like protein 3	-	-	1.942e-12	-

Source	ECs	Term	Type	e value	Identity
Sma3		EC:3.2.-.-	-	1.942e-12	-

Source	Gene names
Sma3	AT5G61250; At5g07830; At5g34940; At5g61250; F13G24.30; GSVIVT00006396001; GSVIVT00016263001; GSVIVT00016264001; GSVIVT00030073001; GSVIVT00032761001; GSVIVT00032762001; LOC_Os03g11320; LOC_Os12g38920; MAF19.29; MFB13.2; MGG23.2; OJ1579_C03.21; OSJNBa0032L

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	lysosomal membrane	GO:0005765	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	hydrolase activity, acting on glycosyl bonds	GO:0016798	Molecular Function	-

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 79	IPR005199	-	-
Sma3	Nodulin-like	IPR010658	-	-
Sma3	Calmodulin-binding domain, plant	IPR012417	-	-
Sma3	Peptidase S1/S6, chymotrypsin/Hap, active site	IPR018114	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G07830.1	AtGUS2, GUS2 glucuronidase 2 chr5:2504168-2506567 FORWARD LENGTH=543	51%
RefSeq	Arabidopsis thaliana	NP_196400.2	Heparanase-like protein 1 [Arabidopsis thaliana]	51%
RefSeq	Populus trichocarpa	XP_002324603.1	predicted protein, partial [Populus trichocarpa]	56%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LM26

Fln msg: Separated hits, possible frame ERROR between 1543 and 1551, your sequence is longer than subject: 544 - 381

Fln protein: MRFFHELGVCVLAIVLFCCLRTCNGQFVSLVVNASAKIAETDENFICATLDWWPPEKCDYGRCSWVGASLLNLDLENPVFANALKAFSPLKIRLGGTLQDKLIYDVGPLSQPCHPFARDASKMFGFSKGCLTMDRWDKLNKFAAKTGSMVAFGLNALYGRYQISADGLWGGPWNSSNARSFIQYTVDHGYKIYAWEFGNELSGGGIGAKVSAIQYAADVTELSKILKDIYKHSPEKPLMVAPDGFFDATWFQELLQQTGPNVLNVVTRHIYNLGAGVDKHLMQKILNPGYLSREATKFKQLQAILQKYGPWASSWVGEAGGAYNGGKHRVSDAFVNSFWYLDxxxxSSTYDTKVYCRQSLIGGNYGLLNTTNFAPNPDYYSALLWHRLMGRGVLSINITGPPYVRTYAHCAKNDTGITLLLINLSNDTKVDILVSAYPNSTMYKRRPNAKESFIHKIKRFVAWAGIKTSKAKDYRMEYHLTPKDGDLHSQTVLLNGAELELTEDGDIPSLDPVMVDSGLPIPVAPSSIAFVSLPGLEISACLQ*

Protein Length: 545

Fln nts: AGTTTGGAATCACACAGTCAGTTTGAAATCCTTCTTCCTGTCATTTTTGCTTTCCAGTAAAGTTATAACATTGGGTGCAAGTGGTTAAGAAAAGTAAGTAGGCTGATAAGATGGTTGGAATGTCACTGGAAATCCCACGAAACAGAAGTTGCCTCCCTCGGCTCAGCTTATAGGCTTTAATTTTGTCAATTTTGATGCCCTGACAATCAAGGCCAACCCATGAATGTGGCTCTGGATAGGAAATTTGAGGTGGATTGAAAGCTTATGCCTTGAGGCGCAAGGAAATTGTATCTTACTGGATAAATTAAGATGGGATGCCGAAGTTCTGCACCAGATCTCATGAGGTTGAATGAATGTGATGGTGTGTTTAACCAGATCTCATGAGGTTGAATGAATATCATGGTGTGTTTATGCATCCAAACACCTGGGAATGAAATTCCCCCTATCCTGTTTGTCACCTGAGTCTCGAAAGCTGCGAATGCATAAACTTTGTGTGAGATTTGGCTAGTTAAAA_-_ATGAGGTTTTTTCATGAATTGGGTGTGTGTGTCTTGGCGATTGTATTATTCTGTTGCTTAAGGACCTGTAATGGGCAGTTTGTCTCTCTTGTTGTAAATGCAAGTGCCAAGATTGCTGAGACGGATGAGAATTTCATATGTGCTACTTTAGATTGGTGGCCTCCTGAAAAATGTGATTATGGCAGATGCAGTTGGGTTGGTGCGTCCCTTCTGAATTTGGATCTCGAAAACCCAGTTTTTGCAAACGCCTTAAAAGCGTTCTCACCACTGAAAATCAGGTTGGGTGGAACTTTGCAAGACAAACTGATATATGACGTTGGACCTCTGTCACAACCCTGTCATCCTTTTGCTAGGGATGCTTCTAAGATGTTTGGGTTCTCAAAAGGTTGTCTTACCATGGACAGATGGGACAAGCTGAACAAGTTTGCCGCAAAAACAGGGTCTATGGTGGCATTTGGCTTAAATGCATTGTATGGTCGGTATCAAATATCTGCGGATGGTCTTTGGGGAGGTCCTTGGAATTCAAGCAATGCTCGCAGCTTTATTCAGTACACCGTCGACCATGGTTATAAGATATATGCATGGGAGTTTGGAAATGAGTTGAGTGGAGGTGGTATTGGTGCAAAAGTTAGTGCTATACAATATGCTGCTGATGTAACTGAGCTTAGTAAGATTTTGAAAGACATATACAAGCATTCTCCTGAGAAACCCTTAATGGTAGCACCAGATGGATTCTTTGATGCGACCTGGTTTCAAGAGCTTCTTCAACAGACTGGGCCAAATGTTCTGAATGTAGTTACACGACATATATACAATCTTGGAGCAGGTGTGGATAAGCATTTGATGCAGAAGATACTTAATCCAGGGTACTTAAGTCGAGAAGCTACAAAGTTCAAACAATTGCAAGCTATTTTGCAGAAATACGGTCCTTGGGCAAGCTCCTGGGTCGGAGAAGCGGGTGGAGCTTATAACGGTGGAAAGCATCGTGTCTCAGATGCTTTTGTGAATAGTTTTTGGTATTTAGATCAxxxxxxxxxATCATCTACATATGATACTAAAGTTTATTGTAGACAAAGCTTGATTGGTGGTAACTATGGCCTTCTTAACACAACAAATTTTGCACCCAATCCTGATTATTATAGTGCACTGCTTTGGCATCGGTTGATGGGGAGAGGTGTGCTTTCCATCAATATTACAGGGCCACCCTATGTGCGCACATATGCTCATTGTGCAAAGAATGATACTGGAATAACTTTACTCTTGATCAACCTCAGCAACGACACAAAAGTTGATATTCTTGTTTCTGCTTATCCAAATTCTACCATGTACAAGAGAAGACCGAATGCAAAAGAGTCTTTTATACATAAGATTAAGAGATTCGTAGCCTGGGCTGGAATCAAAACATCAAAGGCGAAAGATTATAGAATGGAATACCACTTAACACCAAAAGATGGTGACCTTCATAGTCAAACAGTGCTTTTAAATGGTGCAGAGTTGGAGTTAACTGAAGATGGGGATATTCCTTCGTTAGATCCTGTCATGGTTGATTCTGGGCTACCTATTCCAGTAGCTCCTTCATCTATTGCTTTTGTTTCCTTGCCTGGTCTAGAGATCTCTGCTTGTTTACAGTAG___CAATGGTGTAGATATTTATTTGAAACATCAATAGAGCCTTCTAAATTGGGCTATTTATTGAGGTGTATTTGACCAAGTTACCAACAAATCAAGAAAATGTGTTGCTGATGCATGTTGCCACATTGTTTGTCTCAGGGCACGGATTATATAAATTTGTCCAAAGTTTCGAAGAGTCTTT

Fln Alignment:

Contig18175___MDRWDKLNKFAAKTGSMVAFGLNALYGRYQISADGLWGGPWNSSNARSFIQYTVDHGYKIYAWEFGNELSGGGIGAKVSAIQYAADVTELSKILKDIYKHSPEKPLMVAPDGFFDATWFQELLQQTGPNVLNVVTRHIYNLGAGVDKHLMQKILNPGYLSREATKFKQLQAILQKYGPWASSWVGEAGGAYNGGKHRVSDAFVNSFWYLDQxxxSSTYDTKVYCRQSLIGGNYGLLNTTNFAPNPDYYSALLWHRLMGRGVLSINITGPPYVRTYAHCAKNDTGITLLLINLSNDTKVDILVSAYPNSTMYKRRPNAKESFIHKIKRFVAWAGIKTSKAKDYRMEYHLTPKDGDLHSQTVLLNGAELELTEDGDIPSLDPVMVDSGLPIPVAPSSIAFVSLPGLEISAC
B8LM26_______________MSRWDALNAFFNKTGAVVAFGLNALNGK-RWKSDGATDGPWDSSNARDFIQYTVDHGHKIKAWELGNELSGSGVGTSISVEQYAADVIELHGILDGIYKDYEEKPLLIAPDGFFVADWFKEFLNLTGPNIMNVVTHHFYNLGAGVDNDLVEKILNPSYLSQEASTFKGLQAVLQNYGPWSKAWVGETGGAYNSGHNLITNAFVMSFWYLDQxxxASAFSTKSFCRQSLIGGNYGLLNTTTFLPNPDYYSALLWHRLMGTRVLATNSTGTEYLRAYAHCTKSSRGVTLLLINLSRDTGISVQVS----------------------------TGSGTSTDRNSRLEYHLTAKDGDLHSQTVLLNGNILNITPDGEIPSLSAVTVPANRPILVAPLSIAFVNLPYVQFPAC

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
346		11		88	0.923564
360		20		80	0.999996
603		20		80	0.995493
1258			66	33	0.997452
1274		66		33	0.997452
1782	33	33			0.997753
1872		33		66	0.997452
1974	66	33			0.997452
1988	66		33		0.997452
2022		33		66	0.997452
2123		25	75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain