SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene18001

Length: 2205 nt

UniGene Fasta

>sp_v3.0_unigene18001
ATGGGGCCTCCTGAAGTCTCGATTGACTCCACTGGACTGAATTCGTTCCGCGGACATGTTTTCGGATCAAGGAAAGTGTGCTGTGATAACTGAGGCCACGCGGCCAACTATTGTCCACAGGCGGGGCCACGACATTCTCCACGATCCCCTGCTTAACAAGGGCACAGCTTTCCCATTGACAGAGAGAGACCGGCTTGGTCTACGTGGCCTTCTGCCACCAAATGTCATGACTTGGAAGCAACAAATTGATCGCTTTAAGGTTGATCTTAAAAGATTGGAAGAACATGCTAAAGATGGACCATCTGACAATACTGCTTTAGCTAAGTGGAGAATTCTGAACAGATTGCATGATAGGAATGAGACTTTGTATTATAAGGTTTTAATTGATAACATTGCGGAGTATGCGCAAATAATATACACACCTACTGTTGGTATGGTGTGTCAAAACTACAGTGGTTTGTATAGACGGCCGAGAGGAATGTATTTTAGCAGTGCTGATAAGGGGGAAATGATGTCAATGGTCTATAACTGGCCTGCAGAACAGGTTGACATGATTGTGGTTACGGATGGAAGTAGAATACTGGGTCTTGGAGATCTTGGAGTACAGGGTATTGGTATTGCCATTGGAAAGTTAGACTTGTACGTTTCTGCTGCAGGCATAAATCCTCGAAGGGTGCTTCCTGTGATGATTGATTGTGGAACAAATAATGAGAAGCTCCTTGGAGATCCTTTATATCTAGGATTGCCAATCCATAGACTTGATGGAGAAGAATATGTATCAGTTATTGATGAATTCATGGAAGCAGTTTTTTCACGGTGGCCAAAAGTCATAGTGCAGTTTGAGGACTTCCAAAGCAAGTGGGCTTTCAAATTGCTACAACGGTATCGAAATCAATACAGAATGTTTAATGATGATGTTCAGGGAACTGCAGGAGTTGCTGTTGCAGGTCTCCTAGGTGCGGTAAGAGCACAAGGCCGACCAATATCAGACTTTGCAAAACAAAAGATTGTTGTTTCTGGTGCAGGGAGTGCAGGAATAGGAGTCTTAAATGCTGCAAGAAAAGCAATGGCAAGACTTTTAGGTGGTACAGAATCTGCCTTTGAAAATGCAAGGTACCAGTTCTGGGTACTTGATGCAATGGGTTTGGTTACAGAAGCACGGAATGATATTGACCCTGATGTTGCACCATTTGCCAGAAAAGTCGAAGAACTAGGTTCTCAAGGTTTAGCAGAAGGTTCCAAGCTTACTGAAGTGGTAAAAAAAGTTAGGCCAGATGTGTTGATTGGATTAACTGCGGTTGGAGGTTTATTTACAAAAGAGGTTTTGGAAGCCCTTAAAGAATCAACTTGTGTAAGGCCGGTTGTTTTCGCAATGTCAAATCCAACTAGCAATGCTGAATGCACTGCCGAGCAAGCTTTCAACACTGTGGGACACCATGTAGTTTTTGCAAGTGGAAGTCCATTTCTTGATGTGGATCTTGGAAATGGTCAAATTGGGCATTCCAACCAAGGCAACAATATGTACCTTTTCCCAGGGATTGGGCTGGGTACCCTTGTATCTGGTGCTCGTCTCATTTCAGATGGCATGCTTCAGGCTGCAGCAGAATGCTTAGCTGAATGCATGACAGAAGAAGAAGTGCAAAAGGGAATTATATACCCCTCAACATCCAGGATTCGCAATATTACCGTGGAGATAGCTACAGCTGTTGCGAGAGAAGCAGTGGCAGAGGATCTTGCAGAGGGCTACCAAGATAAAGATGCCAGAGAGCTTCATCGCATGTCAAAGGAAGAGACTTTGGAGCATGTGGAAAAGCATATGTGGGTTCCAAACTATGCACCATTGGTTTACCATTAGCAATGAATCCGATCAATGGCAAAAGGGAAGAAATAATCAGTAGCTTTGCAGATTCGTTCCTAAGTTGTTACTATGAAATGAAAATTGTTCGACATATGTACATCTCATTATTCTTGGAGTAGCTGGAGGATTTGTATTCGTGACAGCCTATTTTGCGTAAGAAGCAGGTTGTGGCGTGAATGTCTCAAGTTGCAATGAAAGAATATTACACATTACATGTATTTGATTCAATTATGTCCATTACTTGTTTTCTCTCCGTACTGTAATGAGTATCGTGCTTTCTATACTAGGGCTATACTCTCTAGAATGCTGGTTCACTTAATAAATGCATCTTTTTTTCCTTGTAAAAT

Ace file of the UniGene sp_v3.0_unigene18001

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Malic enzyme n=1 Tax=Picea sitchensis RepID=B8LMR7_PICSI	-	-	86%
FL-Next	sp=Malic enzyme; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Sma3	Malic enzyme	-	-	-

Source	InterPros	Term	Type	Identity
Sma3	Malic enzyme, conserved site	IPR015884	-	-
Sma3	Malic enzyme, N-terminal	IPR012301	-	-
Sma3	Malic enzyme, NAD-binding	IPR012302	-	-
Sma3	Malic oxidoreductase	IPR001891	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G13560.1	NAD-ME1 NAD-dependent malic enzyme 1 chr2:5650089-5655103 FORWARD LENGTH=623	72%
RefSeq	Arabidopsis thaliana	NP_178980.1	NAD-dependent malic enzyme 1 [Arabidopsis thaliana]	72%
RefSeq	Populus trichocarpa	XP_002302483.1	predicted protein [Populus trichocarpa]	78%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LMR7

Fln msg:

Fln protein: MFSDQGKCAVITEATRPTIVHRRGHDILHDPLLNKGTAFPLTERDRLGLRGLLPPNVMTWKQQIDRFKVDLKRLEEHAKDGPSDNTALAKWRILNRLHDRNETLYYKVLIDNIAEYAQIIYTPTVGMVCQNYSGLYRRPRGMYFSSADKGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVSAAGINPRRVLPVMIDCGTNNEKLLGDPLYLGLPIHRLDGEEYVSVIDEFMEAVFSRWPKVIVQFEDFQSKWAFKLLQRYRNQYRMFNDDVQGTAGVAVAGLLGAVRAQGRPISDFAKQKIVVSGAGSAGIGVLNAARKAMARLLGGTESAFENARYQFWVLDAMGLVTEARNDIDPDVAPFARKVEELGSQGLAEGSKLTEVVKKVRPDVLIGLTAVGGLFTKEVLEALKESTCVRPVVFAMSNPTSNAECTAEQAFNTVGHHVVFASGSPFLDVDLGNGQIGHSNQGNNMYLFPGIGLGTLVSGARLISDGMLQAAAECLAECMTEEEVQKGIIYPSTSRIRNITVEIATAVAREAVAEDLAEGYQDKDARELHRMSKEETLEHVEKHMWVPNYAPLVYH*

Protein Length: 601

Fln nts: ATGGGGCCTCCTGAAGTCTCGATTGACTCCACTGGACTGAATTCGTTCCGCGGAC_-_ATGTTTTCGGATCAAGGAAAGTGTGCTGTGATAACTGAGGCCACGCGGCCAACTATTGTCCACAGGCGGGGCCACGACATTCTCCACGATCCCCTGCTTAACAAGGGCACAGCTTTCCCATTGACAGAGAGAGACCGGCTTGGTCTACGTGGCCTTCTGCCACCAAATGTCATGACTTGGAAGCAACAAATTGATCGCTTTAAGGTTGATCTTAAAAGATTGGAAGAACATGCTAAAGATGGACCATCTGACAATACTGCTTTAGCTAAGTGGAGAATTCTGAACAGATTGCATGATAGGAATGAGACTTTGTATTATAAGGTTTTAATTGATAACATTGCGGAGTATGCGCAAATAATATACACACCTACTGTTGGTATGGTGTGTCAAAACTACAGTGGTTTGTATAGACGGCCGAGAGGAATGTATTTTAGCAGTGCTGATAAGGGGGAAATGATGTCAATGGTCTATAACTGGCCTGCAGAACAGGTTGACATGATTGTGGTTACGGATGGAAGTAGAATACTGGGTCTTGGAGATCTTGGAGTACAGGGTATTGGTATTGCCATTGGAAAGTTAGACTTGTACGTTTCTGCTGCAGGCATAAATCCTCGAAGGGTGCTTCCTGTGATGATTGATTGTGGAACAAATAATGAGAAGCTCCTTGGAGATCCTTTATATCTAGGATTGCCAATCCATAGACTTGATGGAGAAGAATATGTATCAGTTATTGATGAATTCATGGAAGCAGTTTTTTCACGGTGGCCAAAAGTCATAGTGCAGTTTGAGGACTTCCAAAGCAAGTGGGCTTTCAAATTGCTACAACGGTATCGAAATCAATACAGAATGTTTAATGATGATGTTCAGGGAACTGCAGGAGTTGCTGTTGCAGGTCTCCTAGGTGCGGTAAGAGCACAAGGCCGACCAATATCAGACTTTGCAAAACAAAAGATTGTTGTTTCTGGTGCAGGGAGTGCAGGAATAGGAGTCTTAAATGCTGCAAGAAAAGCAATGGCAAGACTTTTAGGTGGTACAGAATCTGCCTTTGAAAATGCAAGGTACCAGTTCTGGGTACTTGATGCAATGGGTTTGGTTACAGAAGCACGGAATGATATTGACCCTGATGTTGCACCATTTGCCAGAAAAGTCGAAGAACTAGGTTCTCAAGGTTTAGCAGAAGGTTCCAAGCTTACTGAAGTGGTAAAAAAAGTTAGGCCAGATGTGTTGATTGGATTAACTGCGGTTGGAGGTTTATTTACAAAAGAGGTTTTGGAAGCCCTTAAAGAATCAACTTGTGTAAGGCCGGTTGTTTTCGCAATGTCAAATCCAACTAGCAATGCTGAATGCACTGCCGAGCAAGCTTTCAACACTGTGGGACACCATGTAGTTTTTGCAAGTGGAAGTCCATTTCTTGATGTGGATCTTGGAAATGGTCAAATTGGGCATTCCAACCAAGGCAACAATATGTACCTTTTCCCAGGGATTGGGCTGGGTACCCTTGTATCTGGTGCTCGTCTCATTTCAGATGGCATGCTTCAGGCTGCAGCAGAATGCTTAGCTGAATGCATGACAGAAGAAGAAGTGCAAAAGGGAATTATATACCCCTCAACATCCAGGATTCGCAATATTACCGTGGAGATAGCTACAGCTGTTGCGAGAGAAGCAGTGGCAGAGGATCTTGCAGAGGGCTACCAAGATAAAGATGCCAGAGAGCTTCATCGCATGTCAAAGGAAGAGACTTTGGAGCATGTGGAAAAGCATATGTGGGTTCCAAACTATGCACCATTGGTTTACCATTAG___CAATGAATCCGATCAATGGCAAAAGGGAAGAAATAATCAGTAGCTTTGCAGATTCGTTCCTAAGTTGTTACTATGAAATGAAAATTGTTCGACATATGTACATCTCATTATTCTTGGAGTAGCTGGAGGATTTGTATTCGTGACAGCCTATTTTGCGTAAGAAGCAGGTTGTGGCGTGAATGTCTCAAGTTGCAATGAAAGAATATTACACATTACATGTATTTGATTCAATTATGTCCATTACTTGTTTTCTCTCCGTACTGTAATGAGTATCGTGCTTTCTATACTAGGGCTATACTCTCTAGAATGCTGGTTCACTTAATAAATGCATCTTTTTTTCCTTGTAAAAT

Fln Alignment:

Contig18001___MFSDQGKCAVITEATRPTIVHRRGHDILHDPLLNKGTAFPLTERDXXXXXXXXXXNVMTWKQQIDRFKVDLKRLEEHAKDGPSDNTALAKWRILNRLHDRNETLYYKVLIDNIAEYAQIIYTPTVGMVCQNYSGLYRRPRGMYFSSADKGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVSAAGINPRRVLPVMIDCGTNNEKLLGDPLYLGLPIHRLDGEEYVSVIDEFMEAVFSRWPKVIVQFEDFQSKWAFKLLQRYRNQYRMFNDDXXXXXXXXXXXXXXXXXXXXXPISDFAKQKXXXXXXXXXXXXXLNAARKAMARLLGGTESAFENARYQFWVLDAMGLVTEARNDIDPDVAPFARKVEELGSQGLAEGSKLTEVVKKVRPDVLIGLTAVGGLFTKEVLEALKESTCVRPVVFAMSNPTSNAECTAEQAFNTVGHHVVFASGSPFLDVDLGNGQIGHSNQGNNMYLFPGIGLGTLVSGARLISDGMLQAAAECLAECMTEEEVQKGIIYPSTSRIRNITVEIATAVAREAVAEDLAEGYQDKDARELHRMSKEETLEHVEKHMWVPNYAPLVYH
B8LMR7_______________MSSDQEKCLVITEATRPTIVHRRGLDILHDPLFNKGTAFPLAERDRLGLRGLLPPSVMTWKQQIDRFMVDLKRLEEHAKDGPSDTTALAKWRILNRLHDRNETLYYKVLIDNIAEYAQIIYTPTVGLVCQNYSGLYRRPRGMYFSTADQGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAASGINPQRVLPVMIDCGTNNEKLLGDPLYLGLPIRRLDGEEYVSVIDEFMEAVFARWPNVIVQFEDFQSKWAFKLLQRYRNQYRMFNDDVQGTAGVAIAGLLGAVRAQGRPISDFAKQKIVVSGAGSAGIGVLNAARKAMARFLGGTESAFENARCQFWVLDAMGLVTEARNDIDPDVAPFARKVEELGSQGLREGSQLTEVVKKVRPDVLIGLTGIGGLFTKEVLEALKESTCVRPVVFAMSNPTSNAECTADQAFNTVGHHIVFASGSLFPDVDLGNGQIGHSNQGNNMYLFPGIGLGTLVSGARLISDGMLQAAAECLAECMTKEEVRKGIIYPSVSRIRNITMKVATAVVREAVAEDLAEGYQDKDARELQRLSKEETLEHVEKHMWVPNYSPLVYH

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
1660	25	75			0.996473
1661		75		25	0.996473
1662		75	25		0.996473
1663			25	75	0.996473
1664		75	25		0.996473
1794		7	92		0.999532
1907	88		11		0.923564
2036	80		19		1.0
2055	87			12	1.0
2057	92			7	1.0
2058			92	7	1.0
2059	92			7	1.0
2060	90	2	7		0.999998
2065	92	5			0.990091
2066	5	95			0.997749
2068		87		10	1.0
2072	94	5			0.998578
2076		2	91		0.99441
2079		6		93	0.99903
2194		50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain