SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene15993

Length: 2487 nt

UniGene Fasta

>sp_v3.0_unigene15993
CGTCGTGACCTTGGCTGTATGGGGATTTTTGTTTGGTCTAGCTAGCAGACCAACGGTTCTCTTTACATCCTTTCCTGCTTCCAAGGAAGGTATAAGGAAGTGAGGGCTAGCTCCACATTGAACCGGAACAATGAAGGTCATTGAGAAGCTTCAGAATGCAGAGAAGGAGGGCAAGGTTGTGTTCTCCTTTGAATTCTTCCCTCCGAAAACAGAGGGGGGCGTGGAGAATCTCTTTGATAGAATGGACCAAATGGTAGAACATCAGCCTTCATTTTGTGACATCACATGGGGTGCTGGAGGATCCACTGCAGATCTCACACTGGACATATCTAACAAGATGCAGAACATGATTTGTGTAGAGACTATGATGCATTTAACTTGTACAAACATGCCAGTGGAGAAAATTGACCATGCTTTGACTACCATTAAAGCAAATGGGATTCAAAATGTGTTGGCTCTTCGTGGAGATCCTCCACATGGACAAGACAAGTTTGTCCAAGTTGAAGGTGGATTCGCATGTGCTCTCGATCTGGTTAAGCACATTAGATCGAAGTATGGTGACTATTTTGGGATTACAGTTGCTGGCTATCCAGAGGCCCATCCTGATATGATAAAGAATGACATGGGTGCCACTGAGGAAGATTATCAAAAGGACCTTGCCTATTTGAAATCGAAGGTGGATGCAGGTGCGGATTTAATAATTACACAGTTATTTTATGACACAGACATCTACCTGAAGTTTGTAAATGACTGCCGGCAAATTGGTATAAAATGTCCCATTGTGCCTGGCATCATGCCTATAAACACTTACGGAGGTTTCATTCGAATGGTTGGATTTTGCAAAACTAAGATTCCTTCTGAAATTACTGCTGCATTGGAACCTATCAAAGACAATGAAGAAGCGGTAAGGGCTTATGGAATTCATCTCGGCACTGAAATGTGCAAGAAAATTCTTGCTCATGGAATTAAGACTCTGCATTTGTATACGCTCAACATGGATAAATCAGCGTTGGCAATTCTTGGGAATCTTGGCTTGATAGATATGACAAAGATCAGGAGGTCTTTGCCATGGAGACCAGCAACTAATGTTTTGCGTACTAAAGAGAATGTTCGACCCATATTCTGGGCCAACCGTCCAAGGAGCTACATTTCTCGAACAATTAGTTGGGAACAGTTTCCCCATGGGAGATGGGGGGATACACGTAGCCCAGCTTATGGAGCTCTCAATGACTATCAGTTCTTGCGCCGACGTTCTCGAAGCAAGAAAATTTTGGAGGAATGGGTTGTTCCTCTCAGTTCCATAGAGGATATCCAAGAGAAATTTGCAAACTTTTGTTTGGGGAAGTTGACAAGCAATCCATGGTCGGAGCTTGATGGCCTTCAGCCTGAAACAGTGAAAATCAATGAAAAACTGGAGCAATTAAATCGAAAAGGTTTTCTCACAATAAATAGTCAGCCTGCAGTTAATGGCGAGAAATCCGACTCTCCTTCCATTGGATGGGGAGGACCAGGTGGTTACGTTTATCAAAAGGCCTATGTGGAATTTTTCTGCTCTCCAGAGAAGTTGAATGCATTGGTGGAAAAAGCTAAGAATTCATCTGTTGTCACATATATTGCTGTAAATAAGAAGGGTGCAACAGCAGCTAACATTGGGCCAACTGATGTGAATGCTGTAACTTGGGGAGTTTTCCCTGCAAAGGAGGTCATCCAGCCTACTGTCGTTGATCCTGTGAGTTTCATGGTTTGGAAGGATGAAGCATTCCAGGCCTGGGACCAGGAATGGGCGTCTTTGTATCCCGATGGTGATCCTTCAAGAAAGATCCTTGAAGAGGTACAAAATAGTTACTACTTGGTGAGTGTTGTGGACAATGATTACATCCATGGTGACCTGTTTTCGGTATTTGAAGAATTATAATATCAGTTTTGAAGGTCAATTGGAAGCTGTCACAGTGTGGTTGGTGGTTCTATAGCCATTTAGGTATTACGTACTGGTACATTGGGCTCTATTTTATCTATAGGATTCTTAACTAAAAAGAGAAAACTCTCTTTCTTCCCTGCTATTAATTGAATGTAATGACAAGGCAGAATATAATAGTGGAATTTAGTTCTGTTTCCAAGCTAAATAATATTCTATCTTTTGCATGGACGTGGCAGTATTTAGCAATAGGTGGTTAGTTATACTTATGCCACATGGACGCATGAAAACAAATTTCTCCTTTTTTCACTCTATTGTAATTTTGGTTCTGTATTTGGCAATATGGCATAGTGATTGGGCACCGAAGAATCTGTCAAAGCTGTCAGATGTTTGGAACCCATGGGCAATAAGATAGAACACCCATGCGAGGGTTACCTAAGACACGAGTTTGTTTCAATGCCTCCTTGTGAGTTTGTACTTTTTAAAGCTTTTCCCATAAAATTATTATTTCATAATAAAAACAGTATTGTAAGTTTATTATTATTATGGAGAGGAGAGGTTTTTGGTAGGAC

Ace file of the UniGene sp_v3.0_unigene15993

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Methylenetetrahydrofolate reductase n=1 Tax=Picea sitchensis RepID=B8LKP0_PICSI	-	-	0.0	95%
FL-Next	sp=Methylenetetrahydrofolate reductase; Pinus pinaster (Maritime pine).	-	-	0.0	100%

Source	ECs	Term	Type	e value	Identity
Sma3		EC:1.5.1.2-	-	0.0	-

Source	InterPros	Term	Type	e value	Identity
Sma3	Eukaryotic-type methylenetetrahydrofolate reductase	IPR004621	-	0.0	-
Sma3	Methylenetetrahydrofolate reductase	IPR003171	-	0.0	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G59970.3	MTHFR1 methylenetetrahydrofolate reductase 1 chr3:22151303-22154323 FORWARD LENGTH=592	75%
RefSeq	Arabidopsis thaliana	NP_191556.1	methylenetetrahydrofolate reductase 1 [Arabidopsis thaliana]	75%
RefSeq	Populus trichocarpa	XP_002310366.1	methylenetetrahydrofolate reductase [Populus trichocarpa]	78%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: G3C900

Fln msg:

Fln protein: MKVIEKLQNAEKEGKVVFSFEFFPPKTEGGVENLFDRMDQMVEHQPSFCDITWGAGGSTADLTLDISNKMQNMICVETMMHLTCTNMPVEKIDHALTTIKANGIQNVLALRGDPPHGQDKFVQVEGGFACALDLVKHIRSKYGDYFGITVAGYPEAHPDMIKNDMGATEEDYQKDLAYLKSKVDAGADLIITQLFYDTDIYLKFVNDCRQIGIKCPIVPGIMPINTYGGFIRMVGFCKTKIPSEITAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNMDKSALAILGNLGLIDMTKIRRSLPWRPATNVLRTKENVRPIFWANRPRSYISRTISWEQFPHGRWGDTRSPAYGALNDYQFLRRRSRSKKILEEWVVPLSSIEDIQEKFANFCLGKLTSNPWSELDGLQPETVKINEKLEQLNRKGFLTINSQPAVNGEKSDSPSIGWGGPGGYVYQKAYVEFFCSPEKLNALVEKAKNSSVVTYIAVNKKGATAANIGPTDVNAVTWGVFPAKEVIQPTVVDPVSFMVWKDEAFQAWDQEWASLYPDGDPSRKILEEVQNSYYLVSVVDNDYIHGDLFSVFEEL*

Protein Length: 596

Fln nts: CGTCGTGACCTTGGCTGTATGGGGATTTTTGTTTGGTCTAGCTAGCAGACCAACGGTTCTCTTTACATCCTTTCCTGCTTCCAAGGAAGGTATAAGGAAGTGAGGGCTAGCTCCACATTGAACCGGAACA_-_ATGAAGGTCATTGAGAAGCTTCAGAATGCAGAGAAGGAGGGCAAGGTTGTGTTCTCCTTTGAATTCTTCCCTCCGAAAACAGAGGGGGGCGTGGAGAATCTCTTTGATAGAATGGACCAAATGGTAGAACATCAGCCTTCATTTTGTGACATCACATGGGGTGCTGGAGGATCCACTGCAGATCTCACACTGGACATATCTAACAAGATGCAGAACATGATTTGTGTAGAGACTATGATGCATTTAACTTGTACAAACATGCCAGTGGAGAAAATTGACCATGCTTTGACTACCATTAAAGCAAATGGGATTCAAAATGTGTTGGCTCTTCGTGGAGATCCTCCACATGGACAAGACAAGTTTGTCCAAGTTGAAGGTGGATTCGCATGTGCTCTCGATCTGGTTAAGCACATTAGATCGAAGTATGGTGACTATTTTGGGATTACAGTTGCTGGCTATCCAGAGGCCCATCCTGATATGATAAAGAATGACATGGGTGCCACTGAGGAAGATTATCAAAAGGACCTTGCCTATTTGAAATCGAAGGTGGATGCAGGTGCGGATTTAATAATTACACAGTTATTTTATGACACAGACATCTACCTGAAGTTTGTAAATGACTGCCGGCAAATTGGTATAAAATGTCCCATTGTGCCTGGCATCATGCCTATAAACACTTACGGAGGTTTCATTCGAATGGTTGGATTTTGCAAAACTAAGATTCCTTCTGAAATTACTGCTGCATTGGAACCTATCAAAGACAATGAAGAAGCGGTAAGGGCTTATGGAATTCATCTCGGCACTGAAATGTGCAAGAAAATTCTTGCTCATGGAATTAAGACTCTGCATTTGTATACGCTCAACATGGATAAATCAGCGTTGGCAATTCTTGGGAATCTTGGCTTGATAGATATGACAAAGATCAGGAGGTCTTTGCCATGGAGACCAGCAACTAATGTTTTGCGTACTAAAGAGAATGTTCGACCCATATTCTGGGCCAACCGTCCAAGGAGCTACATTTCTCGAACAATTAGTTGGGAACAGTTTCCCCATGGGAGATGGGGGGATACACGTAGCCCAGCTTATGGAGCTCTCAATGACTATCAGTTCTTGCGCCGACGTTCTCGAAGCAAGAAAATTTTGGAGGAATGGGTTGTTCCTCTCAGTTCCATAGAGGATATCCAAGAGAAATTTGCAAACTTTTGTTTGGGGAAGTTGACAAGCAATCCATGGTCGGAGCTTGATGGCCTTCAGCCTGAAACAGTGAAAATCAATGAAAAACTGGAGCAATTAAATCGAAAAGGTTTTCTCACAATAAATAGTCAGCCTGCAGTTAATGGCGAGAAATCCGACTCTCCTTCCATTGGATGGGGAGGACCAGGTGGTTACGTTTATCAAAAGGCCTATGTGGAATTTTTCTGCTCTCCAGAGAAGTTGAATGCATTGGTGGAAAAAGCTAAGAATTCATCTGTTGTCACATATATTGCTGTAAATAAGAAGGGTGCAACAGCAGCTAACATTGGGCCAACTGATGTGAATGCTGTAACTTGGGGAGTTTTCCCTGCAAAGGAGGTCATCCAGCCTACTGTCGTTGATCCTGTGAGTTTCATGGTTTGGAAGGATGAAGCATTCCAGGCCTGGGACCAGGAATGGGCGTCTTTGTATCCCGATGGTGATCCTTCAAGAAAGATCCTTGAAGAGGTACAAAATAGTTACTACTTGGTGAGTGTTGTGGACAATGATTACATCCATGGTGACCTGTTTTCGGTATTTGAAGAATTATAA___TATCAGTTTTGAAGGTCAATTGGAAGCTGTCACAGTGTGGTTGGTGGTTCTATAGCCATTTAGGTATTACGTACTGGTACATTGGGCTCTATTTTATCTATAGGATTCTTAACTAAAAAGAGAAAACTCTCTTTCTTCCCTGCTATTAATTGAATGTAATGACAAGGCAGAATATAATAGTGGAATTTAGTTCTGTTTCCAAGCTAAATAATATTCTATCTTTTGCATGGACGTGGCAGTATTTAGCAATAGGTGGTTAGTTATACTTATGCCACATGGACGCATGAAAACAAATTTCTCCTTTTTTCACTCTATTGTAATTTTGGTTCTGTATTTGGCAATATGGCATAGTGATTGGGCACCGAAGAATCTGTCAAAGCTGTCAGATGTTTGGAACCCATGGGCAATAAGATAGAACACCCATGCGAGGGTTACCTAAGACACGAGTTTGTTTCAATGCCTCCTTGTGAGTTTGTACTTTTTAAAGCTTTTCCCATAAAATTATTATTTCATAATAAAAACAGTATTGTAAGTTTATTATTATTATGGAGAGGAGAGGTTTTTGGTAGGAC

Fln Alignment:

Contig15993___MKVIEKLQNAEKEGKVVFSFEFFPPKTEGGVENLFDRMDQMVEHQPSFCDITWGAGGSTADLTLDISNKMQNMICVETMMHLTCTNMPVEKIDHALTTIKANGIQNVLALRGDPPHGQDKFVQVEGGFACALDLVKHIRSKYGDYFGITVAGYPEAHPDMIKNDMGATEEDYQKDLAYLKSKVDAGADLIITQLFYDTDIYLKFVNDCRQIGIKCPIVPGIMPINTYGGFIRMVGFCKTKIPSEITAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNMDKSALAILGNLGLIDMTKIRRSLPWRPATNVLRTKENVRPIFWANRPRSYISRTISWEQFPHGRWGDTRSPAYGALNDYQFLRRRSRSKKILEEWVVPLSSIEDIQEKFANFCLGKLTSNPWSELDGLQPETVKINEKLEQLNRKGFLTINSQPAVNGEKSDSPSIGWGGPGGYVYQKAYVEFFCSPEKLNALVEKAKNSSVVTYIAVNKKGATAANIGPTDVNAVTWGVFPAKEVIQPTVVDPVSFMVWKDEAFQAWDQEWASLYPDGDPSRKILEEVQNSYYLVSVVDNDYIHGDLFSVFEEL
G3C900_______________MKVIEKLQNAEKEGKVVFSFEFFPPKTEGGVENLFDRMDQMVEHQPSFCDITWGAGGSTADLTLDISNKMQNMICVETMMHLTCTNMPVEKIDHALTTIKANGIQNVLALRGDPPHGQDKFVQVEGGFACALDLVKHIRSKYGDYFGITVAGYPEAHPDMIKNDMGATEEDYQKDLAYLKSKVDAGADLIITQLFYDTDIYLKFVNDCRQIGIKCPIVPGIMPINTYGGFIRMVGFCKTKIPSEITAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNMDKSALAILGNLGLIDMTKIRRSLPWRPATNVLRTKENVRPIFWANRPRSYISRTISWEQFPHGRWGDTRSPAYGALNDYQFLRRRSRSKKILEEWVVPLSSIEDIQEKFANFCLGKLTSNPWSELDGLQPETVKINEKLEQLNRKGFLTINSQPAVNGEKSDSPSIGWGGPGGYVYQKAYVEFFCSPEKLNALVEKAKNSSVVTYIAVNKKGATAANIGPTDVNAVTWGVFPAKEVIQPTVVDPVSFMVWKDEAFQAWDQEWASLYPDGDPSRKILEEVQNSYYLVSVVDNDYIHGDLFSVFEEL

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
84	83		16		0.994512
89			88	11	0.923564
91			11	88	0.923564
92	88		11		0.923564
93			11	88	0.923564
216			85	14	0.993533
466	87		12		0.999964
660		90		10	0.90298
2017	92		7		0.999602
2178	93		6		0.999264
2323		3		96	0.993037
2431		11		88	0.923564
2433	87			12	0.992554

SSRs (tot: 1)

Start position	End position	Sequence	Length
2450	2462	TTA TTA TTA TTA T	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain