SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene15714

Length: 1485 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene15714

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	serine/threonine protein kinase isolog [Arabidopsis thaliana] gb\|AAO30076.1\| serine/threonine protein kinase isolog [Arabidopsis thaliana]	-	-	0.0	45%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	42%
Sma3	Chromosome chr10 scaffold_81, whole genome shotgun sequence	-	-	3.661e-11	-

Source	ECs	Term	Type	e value	Identity
Sma3	Mitogen-activated protein kinase kinase kinase.	EC:2.7.11.25	-	3.3e-07	-

Source	Gene names
Sma3	AT1G07650; AT1G53420; AT1G53430; AT1G53440; AT1G56145; AT3G14840; AT4g01330; AT5G48740; A_IG002N01.22; At1g01540; At1g11050; At1g56145; At1g56720; At1g70460; At1g76370; At2g42960; At3g59110; At4g04500; At5g40380; B1065E10.29; B1153E06.9; CRK37; CRK42; F14

Source	GOs	Term	Type	Identity
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	aspartic-type endopeptidase activity	GO:0004190	Molecular Function	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	protein tyrosine kinase activity	GO:0004713	Molecular Function	-
Sma3	receptor activity	GO:0004872	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	sugar binding	GO:0005529	Molecular Function	-
Sma3	metal ion transmembrane transporter activity	GO:0046873	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	proteolysis	GO:0006508	Biological Process	-
Sma3	cell wall macromolecule catabolic process	GO:0016998	Biological Process	-
Sma3	metal ion transport	GO:0030001	Biological Process	-
Sma3	recognition of pollen	GO:0048544	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Regulator of chromosome condensation, RCC1	IPR000408	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Epidermal growth factor-like domain	IPR000742	-	-
Sma3	S-locus glycoprotein	IPR000858	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Bulb-type lectin domain	IPR001480	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	Alpha-isopropylmalate/homocitrate synthase, conserved site	IPR002034	-	-
Sma3	Chaperonin TCP-1, conserved site	IPR002194	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Lipocalin	IPR002345	-	-
Sma3	Peptidoglycan-binding Lysin subgroup	IPR002482	-	-
Sma3	Mg2+ transporter protein, CorA-like/Zinc transport protein ZntB	IPR002523	-	-
Sma3	Gnk2-homologous domain	IPR002902	-	-
Sma3	Apple-like	IPR003609	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Leucine-rich repeat-containing N-terminal, type 2	IPR013210	-	-
Sma3	PAN-2 domain	IPR013227	-	-
Sma3	SKG6/AXL2 alpha-helix transmembrane domain	IPR014805	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Beta-ketoacyl synthase, active site	IPR018201	-	-
Sma3	Heat shock protein DnaJ, conserved site	IPR018253	-	-
Sma3	Peptidoglycan-binding lysin domain	IPR018392	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G11050.1	Protein kinase superfamily protein chr1:3681892-3683769 FORWARD LENGTH=625	45%
RefSeq	Arabidopsis thaliana	NP_172572.1	putative receptor-like protein kinase [Arabidopsis thaliana]	45%
RefSeq	Populus trichocarpa	XP_002306651.1	predicted protein [Populus trichocarpa]	48%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LPC1

Fln msg: Distance to subject end: 137 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 483 - 611

Fln protein: MKMESILLLLLLLNRAMASDTECPMALDYVHQLLWDKSSCQTPINKANCCQILRGLLGIGLAQYLRDASKFELPSNASAVSCFEQFQLQLDSVGLSPDLLGNCFVNTSEFVSSADLCAGIQTKEDWLRKVKNTPMDSACKGDLADVGACLQCTQSGGLVTKILVDTYKNLTNEATSMKCFYFTCLYAAGVVNEFGPKNINTAQCILRVPFVQTASDSRVLAYALMGTALAAALICGLGIFYTLWVRRIDPKAILHRRFVKRNQKVLKTAMKPNTGAVWFDIRLIRDATDNFSETNFIGQGGFGTVYRGTLPDGRQIAVKRIRNCTREGDSEFLNEVEIINNIKHRNLVVLRGCCVASDEKEGHQRFLIYDYMPNGSLADHIFGRESKARPLSWPQRRNIVLGTAKGLAYLHDGVEPAIYHRDIKATNILLDDEMNACVADFGLARMMTKGGESHITTKVAGTHGYLAPEYALYGQLTDKSDVY

Protein Length: 484

Fln nts: ATGGGGGAAGAAACAGTAGCGTTTGTGAGAACTAA_-_ATGAAAATGGAGAGCATCCTGCTGCTACTGTTGCTGTTGAATAGGGCGATGGCCTCTGATACGGAATGTCCGATGGCTCTTGATTATGTCCATCAATTGCTGTGGGACAAGTCTTCCTGTCAAACGCCGATTAATAAAGCCAATTGCTGCCAGATATTGCGTGGATTGCTCGGCATAGGATTGGCTCAGTATTTGAGAGATGCATCCAAATTCGAGCTCCCGAGTAATGCCTCTGCCGTGTCCTGTTTCGAGCAATTTCAACTGCAGCTGGACTCTGTGGGTCTCTCACCAGACTTGCTGGGAAACTGCTTCGTGAATACTTCTGAGTTTGTTTCTAGCGCAGACCTTTGCGCAGGCATACAGACCAAGGAGGACTGGCTTCGCAAAGTGAAGAATACTCCCATGGATTCGGCCTGCAAAGGCGACCTCGCAGATGTAGGCGCCTGTCTGCAGTGCACGCAAAGTGGAGGTCTCGTTACCAAGATACTGGTGGACACCTACAAAAATTTGACGAACGAGGCAACAAGTATGAAATGCTTTTATTTCACTTGTCTGTATGCGGCCGGAGTGGTTAATGAGTTCGGCCCCAAGAACATAAATACTGCTCAGTGTATCCTCCGCGTGCCCTTTGTCCAAACCGCGTCGGATAGCCGAGTTTTGGCATACGCTTTGATGGGCACTGCCCTGGCCGCTGCCTTGATCTGTGGTCTAGGGATTTTCTACACTTTGTGGGTGAGGAGGATTGATCCTAAGGCTATACTGCACAGGCGATTCGTAAAGCGCAATCAGAAAGTTCTAAAGACAGCCATGAAACCCAACACCGGGGCAGTGTGGTTCGACATTCGATTGATCAGGGACGCCACCGATAATTTCAGTGAAACCAATTTCATCGGTCAAGGGGGTTTCGGTACAGTATACAGAGGAACGCTCCCGGATGGTCGGCAGATAGCTGTGAAGAGAATCAGAAACTGCACGCGCGAGGGGGATTCCGAATTCCTCAACGAAGTGGAGATCATTAACAATATCAAGCACAGGAATCTGGTTGTTTTGAGGGGATGCTGCGTAGCCAGCGATGAGAAGGAAGGGCACCAGCGGTTTCTGATATATGATTATATGCCCAATGGAAGTCTGGCCGATCATATATTTGGACGAGAGTCGAAGGCGCGGCCTTTGAGCTGGCCGCAGCGTAGGAACATCGTCCTTGGAACAGCCAAAGGGCTGGCTTATCTGCACGACGGTGTCGAGCCCGCGATTTATCACAGAGACATCAAAGCCACTAACATTCTGCTGGATGACGAAATGAATGCTTGTGTGGCCGATTTTGGTCTGGCGAGAATGATGACTAAAGGAGGAGAATCCCATATCACCACTAAAGTAGCAGGGACGCATGGCTATTTGGCCCCTGAATATGCTCTCTATGGTCAGCTCACAGATAAGAGCGATGTATATA

Fln Alignment:

Contig15714___LGIGLAQYLRDASKFELPSNASAVSCFEQFQLQLDSVGLSPDLLGNCFVNTSEFVSSADLCAGIQTKEDWLRKVK---NTPMDSACKGDLADVGACLQCTQSGGLVTKI-LVDTYKNLTNEATSMKCFYFTCLYAAGVVNEFGPKNINTAQCILRVPFVQTASDSRVXXXXXXXXXXXXXXICGLG-IFYTLWVRRIDPKAILHRRFVKRNQKVLKT-------AMKPNTGAVWFDIRLIRDATDNFSETNFIGQGGFGTVYRGTLPDGRQIAVKRIRNCTREGDSEFLNEVEIINNIKHRNLVVLRGCCVASDEKEGHQRFLIYDYMPNGSLADHIFGRESKARPLSWPQRRNIVLGTAKGLAYLHDGVEPAIYHRDIKATNILLDDEMNACVADFGLARMMTKGGESHITTKVAGTHGYLAPEYALYGQLTDKSDVY
B8LPC1_______________LHLVLAEYLRTEGLFLPPANTSE-SCWSAFHTELNSQDPSFDFRKSCGFDNS---SISQGCMNITTQQEFEKQVSLISRQTLTKACNQTLTS-SPCAQCTAALTSTQAISLPGPSGNLTD------CSAYSSIYAAAILNKAGPTDKNTAWCLF---FIGLNLSSGKNSAWIYGAVAAGVSVCVLGAVVGFLYCRR--------RRARMEKKKVLAEFEASDPCSMNPNSTLVRFTIEDIRAATKNFARENIVGTGGFGNVYKGVLADGSLVAVKRFKNCSPAGDPEFVHEVDVISSIRHRNLVALRGFCVAPGSLEGHQRILVCEFIPNRSLHDNLFDHRRSERRLDWPTRCQIAVGMARGLAYLHHEIQPGIIHRDIKASNILLDENFNARVADFGLAKFAPE-GVSHLSTRVAGTLGYVAPEYALYGQLTEKSDVY

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
114	33	66			0.997452
115	33			66	0.997452
376		33	66		1.0
404	75			25	0.996473
406		50	50		1.0
844	25	75			0.996473
848			75	25	0.996473
893	33		66		1.0
958		80	20		0.995493
959	80		20		0.995493
1240	33	66			0.997452
1242	33			66	0.997452
1246	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain