SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene15046

Length: 1692 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene15046

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Alpha-amylase, putative n=1 Tax=Ricinus communis RepID=B9R8H8_RICCO	-	-	70%
FL-Next	sp=Probable alpha-amylase 2; Arabidopsis thaliana (Mouse-ear cress).	-	-	68%
Sma3	Alpha-amylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Alpha-amylase.	EC:3.2.1.1	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Starch and sucrose metabolism	00500		0.0	%
Sma3	Metabolic pathways	01100		0.0	%
Sma3		EC:3.2.1.6-	-	2.722e-17	-

Source	Gene names
Sma3	AAM1; ALPHA-AMY3; AMY1.1; AMY1.2; AMY1.3; AMY1.4; AMY1.5; AMY1.6; AMY1.7; AMY10; AMY1A; AMY2; AMY2A; AMY3; AMY3A; AMY3B; AMY3D; AMY3E; AMY4; AMY5; AMY9; AMYA1; AMYA2; AMYC2; AT4g25000; Aamy1; Aamy2; Aamy3; Amy23; Amy32b; Amy46; AmyF1; At1g69830; At1g76130

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	catalytic activity	GO:0003824	Molecular Function	-
Sma3	alpha-amylase activity	GO:0004556	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	cation binding	GO:0043169	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	starch catabolic process	GO:0005983	Biological Process	-
Sma3	sucrose catabolic process	GO:0005987	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Phosphotransferase system, HPr histidine phosphorylation site	IPR001020	-	-
Sma3	Phosphoribosylglycinamide formyltransferase, active site	IPR001555	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Glycoside hydrolase, family 13	IPR006046	-	-
Sma3	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	-
Sma3	Glycosyl hydrolase, family 13, subfamily, catalytic domain	IPR006589	-	-
Sma3	Alpha-amylase, C-terminal beta-sheet	IPR012850	-	-
Sma3	Alpha-amylase, plant	IPR013775	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT1G76130.1	ATAMY2, AMY2 alpha-amylase-like 2 chr1:28561647-28563914 FORWARD LENGTH=413	68%
RefSeq	Arabidopsis thaliana	NP_177740.1	alpha-amylase-like 2 [Arabidopsis thaliana]	68%
RefSeq	Populus trichocarpa	XP_002301935.1	predicted protein [Populus trichocarpa]	69%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q8LFG1

Fln msg:

Fln protein: MGYPVQEVSKEHDQWAGFVEGESVLQRGREILLQGFNWESHKYKWWPNLEAKIPQIAKAGFTSVWLPPAFELLLHPKVTYPETFIL*TLHMVLEYQLKSLLMTMRNKNVRAMADIVIHRRMGCSQGFGGLHNRYDGLTVPWDERAVTRCTGGLGNWSTGDNFHGVPNIDHTQDFVRKDIKDWLRWLRRSVGFQDFRFDFAKGYAAKFVKEYIEASTPMFAVGEYWDSCNYTPPSYHLDKNQDSHRQRIINWIDGTSGISAAFDFTTKGILQEAVKGQCWRLRDHQGKPPGVLGWWPSRAVTFIENHDTGSTQGHWPFPCDHIMEGYAYILTHPGIPTVFYDHFFDWGSSIQNEIIKLMSIRRTQDLNSRSSIEILEASSTVYAAIIGRKVCMKIGDGSWCPHGREWQLATCGHRYAVWHK*

Protein Length: 422

Fln nts: CTCGCAACGAATCTATTTACTGATTGCTTTTTCATCAATTTAAGGCAAACTACAGCGAGCTAAGTGTATGGGCCTACTCAAGTGAGGAGCAAATTCTGGGATACCCTGCTGGGACTTGATCCCTTTTGAACAGGAATTATTTAATTTTCCTAATTATTTTAGTTTGCAAGGAAACTTGACTACTCCATCGATTTGTTTACAGTGATCGAAA_-_ATGGGCTATCCAGTTCAGGAGGTATCCAAGGAACACGATCAATGGGCAGGATTTGTTGAAGGTGAAAGTGTGCTTCAAAGAGGAAGGGAGATTCTTCTCCAGGGTTTTAACTGGGAATCACATAAATACAAGTGGTGGCCAAATTTGGAAGCAAAGATCCCGCAGATTGCTAAAGCAGGATTTACATCTGTATGGCTGCCACCTGCTTTTGAATTACTGCTGCACCCCAAGGTTACTTACCCCGAAACATTTATTCTCTGAACTCTGCATATGGTTCTAGAATATCAGCTGAAAAGCTTACTTATGACAATGCGAAATAAAAATGTGAGAGCCATGGCTGACATAGTTATCCATCGTCGCATGGGCTGCTCTCAGGGGTTTGGAGGCTTGCATAATCGCTATGATGGCTTGACAGTGCCTTGGGATGAACGTGCTGTTACACGTTGTACTGGTGGACTTGGAAACTGGAGCACAGGGGATAATTTTCATGGAGTACCAAACATTGATCACACCCAAGATTTTGTACGCAAAGACATCAAAGATTGGTTAAGGTGGCTTCGGAGAAGTGTTGGTTTTCAAGATTTTCGATTTGACTTTGCCAAGGGTTATGCAGCAAAATTTGTGAAAGAATATATCGAGGCATCGACCCCCATGTTTGCTGTTGGGGAATATTGGGATTCGTGCAATTATACTCCTCCTAGCTATCATCTTGATAAGAATCAAGATAGCCATAGACAAAGAATAATCAATTGGATTGATGGCACTAGTGGCATCTCTGCGGCATTTGATTTTACAACAAAGGGCATTCTTCAGGAGGCAGTGAAAGGACAATGTTGGCGTTTACGTGATCACCAGGGAAAGCCTCCTGGAGTTCTTGGCTGGTGGCCTTCAAGGGCTGTAACTTTTATTGAAAACCACGACACTGGATCAACACAGGGTCATTGGCCTTTTCCATGTGATCATATTATGGAGGGCTATGCTTACATCTTGACACACCCTGGAATACCAACAGTCTTCTATGACCATTTTTTTGATTGGGGAAGCTCAATACAAAACGAGATTATAAAGCTGATGAGTATTCGGAGAACACAAGATTTAAACAGCCGCTCTTCAATAGAGATTTTAGAGGCCAGTTCAACTGTTTATGCTGCAATTATTGGAAGGAAAGTTTGCATGAAGATTGGAGATGGCTCATGGTGTCCACATGGCAGGGAGTGGCAGCTAGCTACTTGTGGTCATAGATATGCTGTCTGGCACAAGTAA___CATTGTTTTTGCATGTATTTCTAGTTGCTAGATACTAGTGTCTTGTAGTGAATTTATTTCTAGTGGCTAGATACTAGTATTTTGTAGTGAATTAATGTATGTCTATTTTTAACTGTGGAAATGAGATGCATGGGTACGATATACAGATTACATAAAATTTTCTACACGGCTATAGACCTACTCTAGAGGTCTATGTTATGAGCACATCCACGTTGAAA

Fln Alignment:

Contig15046___VLQRGREILLQGFNWESHKYKWWPNLEAKIPQIAKAGFTSVWLPPAFELLLHPKVTYPETFIL*TLHMVLEYQLKSLLMTMRNKNVRAMADIVIHRRMGCSQGFGGLHNRYDGLTVPWDERAVTRCTGGLGNWSTGDNFHGVPNIDHTQDFVRKDIKDWLRWLRRSVGFQDFRFDFAKGYAAKFVKEYIEASTPMFAVGEYWDSCNYTPPSYHLDKNQDSHRQRIINWIDGTSGISAAFDFTTKGILQEAVKGQCWRLRDHQGKPPGVLGWWPSRAVTFIENHDTGSTQGHWPFPCDHIMEGYAYILTHPGIPTVFYDHFFDWGSSIQNEIIKLMSIRRTQDLNSRSSIEILEASSTVYAAIIGRKVCMKIGDGSWCPHGREWQLATCGHRYAVWHK
Q8LFG1_______________VIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLA-PEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYN--GHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
68	66		33		0.997452
69			33	66	0.997452
71			66	33	0.997452
217	20		80		0.995493
755	16		83		0.994512
762		14		85	0.993533
763	85		14		0.993533
764	85			14	0.993533
765		14	85		0.993533
769	14		85		0.993533
771		14		85	0.993533
773		85		14	0.993533
776	85	14			0.993533
777			85	14	0.993533
778	85		14		0.993533
779	85			14	0.993533
780		14	85		0.993533
781	14			85	0.993533
782		14	85		0.993533
784		14		85	0.993533
785	14		85		0.993533
878	12		87		0.992554
933	85		14		0.993533
961	14	85			0.993533
1031	10		90		0.90298
1032		90		10	0.90298
1033	90		10		0.90298
1035			10	90	0.90298
1109	12		87		0.992554
1238		87		12	0.992554
1482	25		75		1.0
1516		50		50	1.0
1558		50		50	1.0
1576	75			25	0.996473
1627	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain