SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene14147

Length: 2167 nt

UniGene Fasta

>sp_v3.0_unigene14147
ATGGGGAGAACCGATCGATGCAATCCATCCCATCCAGGGAATACTCACCAGTATAGTAACGCCCCCGCTCCATTCATTCAATTTCGGTTTTCTGTCACATAGTCACCATGTGTGGAATCCTAGCAGTGTTCGGTTGCTTGGACGCATCGCAGGCCAAGCGTGTTCGGGTACTGGAGCTCTCGCGCAGGTTACGACATAGAGGCCCTGACTGGAGTGGACTGTATCAGAGCGGGCATTCCTTTCTTGCTCACCAGAGGTTGGCCATCGTAGATCCTGCCTCTGGGGATCAACCCCTCTACAATGAAGATAGACAAATAGTTGTTACTGTTAATGGAGAAATATATAACCACGAGAAACTGAGGGAACAGCTCAAATCTCACAAGTTTCGGACTGGAAGCGATTGTGAAATTGTAGCTCATCTCTATGAGGACTATGGCGAGGAGTTCGTGAACATGTCGTGATCGTAGGTTCTCTCTCGTTTTGCTTGACACGCGTGATCAGAGCTTCATAGCTGCTCGAGATGCTTTTGGAATCACTCCCCTCTACATAGGTTGGGGATTTGATGGATCTGTTTGGTTTGCGTCAGAGATGAAAGCTTTGCACGATGACTGTGAAAGTTTTGAGTCATTTCCTCCTGGACATATATATTCCAGCAAACAAGGCGGACTACGGAGATGGTACAACCCACCTTGGTATTCAGAAAATGTTATACCGTCTTCTCCATATGACTCTTTGGTTTTAAGACGGGCATTTGAGAAAGCAGTCATAAAAAGGCTGATGACGGATGTTCCATTTGGAGTACTGTTATCAGGAGGGCTTGACTCATCCTTAGTTGCAGCTGTTGCGTCGCGCTATTTGGCTGCTACCAAGGCTGGAAAACAATGAGGAGACAAACTACATTCTTTCTGTGTTGGATTAAAGGGATCGCCAGATTTAAAGGCTGCCAAAGAGGTAGCAGAGTACCTTGGTACTTTTCATCATGAATTCCATTTTACTGTACAGGATGGTATAGATGCAATCGAGGACGTTATATACCATGTCGAAACATATGACGTGACAACTATCAGAGCGAGCACACCCATGTTTTTAATGTCTCGGAAAATCAAGTCGCTAGGAGTGAAGATGGTTCTGTCTGGAGAAGGATCAGATGAGATATTTGGTGGCTATTTGTACTTCCACAAGGCTCCTAACAAAGAGGAATTCCACCAGGAAACATGTCGTAAGATAAAGGCCCTTCACATGTATGATTGCTTGAGGGCAAATAAGTCCACATCTGCATGGGGTGTGGAGGCCCGTGTGCCATTTCTAGATAAGGAATTCGTCAATGTTGCCATGGACATTGATCCCGAGTGGAAGATGGTAAAGCCTGACATTGGGCGCATTGAGAAGTGGGTCTTGCGGAGGGCATTCGACGACGAGCAGAATCCTTATCTTCCCAAGCACATTCTGTACAGACAGAAGGAGCAATTCAGCGATGGGGTTGGCTACAGCTGGATTGATGGCTTGAAATCACATGCGGCAGAGCATGTGACAGATACGATGCTGCGCAATGCCAAATACATATTCCCCCATAATACTCCAACTACGAAGGAGGCGTACTACTACAGGATGATATTTGAAAGATTCTATCCTCAGAACGCGGCACGGTTGACGGTCCCGGGCGGGCCAAGCGTGGCTTGTAGCACCGCAAAGGCAGTGGAATGGGATGCTGCGTGGGCTCACAATCTGGACCCCTCCGGCAGGGCTGCTCTAGGCGTCCATGCCTCCGCGTACGAAGAGTGTAACGCAGGTGCGAATGATGGCACCGGCAATGGGAACGTCGATACATTCAGCAAGATGCCAGAAAGGGAAGAGAGTGTAGGTGCCCGCGAAGTGCTGATCAAAGGTTGAATTGTAAAGTATTCGTATCGACTAGGGTAGCCACGTTACTATGTAATAATCTGTACACAGAAATGGTTGGGGAAGAGTATGTCTCTGTTGTACATCAAAGTGAGAGCTTGTGATTACTGTATTTCCATTGGCTGGTGTTAGTTTTCAAAGATTTCAATGTCGCAGCACTTTGTGATATTTAAAGAACGGTTTTATTTGGATGGGTTCAAAGTGAATGTGTTAAGTAAAAAATATGTATTTTGCTTTAAAAGTTATAGAAATTTTAGTTATATTTAGA

Ace file of the UniGene sp_v3.0_unigene14147

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Asparagine synthetase n=3 Tax=Pinaceae RepID=E7EAQ2_PINPS	-	-	0.0	93%
FL-Next	sp=Asparagine synthetase; Pinus pinaster (Maritime pine).	-	-	0.0	100%

Source	ECs	Term	Type	e value	Identity
Sma3	Asparagine synthase (glutamine-hydrolyzing).	EC:6.3.5.4	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Alanine, aspartate and glutamate metabolism	00250	%
Sma3	Nitrogen metabolism	00910	%
Sma3	Metabolic pathways	01100	%
Sma3	Biosynthesis of secondary metabolites	01110	%

Source	Gene names
Sma3	asn1;

Source	GOs	Term	Type	e value	Identity
Sma3	asparagine synthase (glutamine-hydrolyzing) activity	GO:0004066	Molecular Function	0.0	-
Sma3	asparagine biosynthetic process	GO:0006529	Biological Process	0.0	-

Source	InterPros	Term	Type	Identity
Sma3	Asparagine synthase, glutamine-hydrolyzing	IPR006426	-	-
Sma3	Asparagine synthase	IPR001962	-	-
Sma3	Glutamine amidotransferase, class-II	IPR000583	-	-
Sma3	Glutamine amidotransferase, type II	IPR017932	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G10240.1	ASN3 asparagine synthetase 3 chr5:3212934-3216418 REVERSE LENGTH=578	84%
RefSeq	Arabidopsis thaliana	NP_001031864.1	asparagine synthetase 3 [Arabidopsis thaliana]	84%
RefSeq	Populus trichocarpa	XP_002306310.1	predicted protein, partial [Populus trichocarpa]	84%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: E7EAQ2

Fln msg: Separated hits, possible frame ERROR between 455 and 460,

Fln protein: MCGILAVFGCLDASQAKRVRVLELSRRLRHRGPDWSGLYQSGHSFLAHQRLAIVDPASGDQPLYNEDRQIVVTVNGEIYNHEKLREQLKSHKFRTGSDCEIVAHLYEDYGEEFVNxxxDRRFSLVLLDTRDQSFIAARDAFGITPLYIGWGFDGSVWFASEMKALHDDCESFESFPPGHIYSSKQGGLRRWYNPPWYSENVIPSSPYDSLVLRRAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVASRYLAATKAGKQ*GDKLHSFCVGLKGSPDLKAAKEVAEYLGTFHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFVNVAMDIDPEWKMVKPDIGRIEKWVLRRAFDDEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLRNAKYIFPHNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAAWAHNLDPSGRAALGVHASAYEECNAGANDGTGNGNVDTFSKMPEREESVGAREVLIKG*

Protein Length: 596

Fln nts: ATGGGGAGAACCGATCGATGCAATCCATCCCATCCAGGGAATACTCACCAGTATAGTAACGCCCCCGCTCCATTCATTCAATTTCGGTTTTCTGTCACATAGTCACC_-_ATGTGTGGAATCCTAGCAGTGTTCGGTTGCTTGGACGCATCGCAGGCCAAGCGTGTTCGGGTACTGGAGCTCTCGCGCAGGTTACGACATAGAGGCCCTGACTGGAGTGGACTGTATCAGAGCGGGCATTCCTTTCTTGCTCACCAGAGGTTGGCCATCGTAGATCCTGCCTCTGGGGATCAACCCCTCTACAATGAAGATAGACAAATAGTTGTTACTGTTAATGGAGAAATATATAACCACGAGAAACTGAGGGAACAGCTCAAATCTCACAAGTTTCGGACTGGAAGCGATTGTGAAATTGTAGCTCATCTCTATGAGGACTATGGCGAGGAGTTCGTGAACATxxxxxxTGATCGTAGGTTCTCTCTCGTTTTGCTTGACACGCGTGATCAGAGCTTCATAGCTGCTCGAGATGCTTTTGGAATCACTCCCCTCTACATAGGTTGGGGATTTGATGGATCTGTTTGGTTTGCGTCAGAGATGAAAGCTTTGCACGATGACTGTGAAAGTTTTGAGTCATTTCCTCCTGGACATATATATTCCAGCAAACAAGGCGGACTACGGAGATGGTACAACCCACCTTGGTATTCAGAAAATGTTATACCGTCTTCTCCATATGACTCTTTGGTTTTAAGACGGGCATTTGAGAAAGCAGTCATAAAAAGGCTGATGACGGATGTTCCATTTGGAGTACTGTTATCAGGAGGGCTTGACTCATCCTTAGTTGCAGCTGTTGCGTCGCGCTATTTGGCTGCTACCAAGGCTGGAAAACAATGAGGAGACAAACTACATTCTTTCTGTGTTGGATTAAAGGGATCGCCAGATTTAAAGGCTGCCAAAGAGGTAGCAGAGTACCTTGGTACTTTTCATCATGAATTCCATTTTACTGTACAGGATGGTATAGATGCAATCGAGGACGTTATATACCATGTCGAAACATATGACGTGACAACTATCAGAGCGAGCACACCCATGTTTTTAATGTCTCGGAAAATCAAGTCGCTAGGAGTGAAGATGGTTCTGTCTGGAGAAGGATCAGATGAGATATTTGGTGGCTATTTGTACTTCCACAAGGCTCCTAACAAAGAGGAATTCCACCAGGAAACATGTCGTAAGATAAAGGCCCTTCACATGTATGATTGCTTGAGGGCAAATAAGTCCACATCTGCATGGGGTGTGGAGGCCCGTGTGCCATTTCTAGATAAGGAATTCGTCAATGTTGCCATGGACATTGATCCCGAGTGGAAGATGGTAAAGCCTGACATTGGGCGCATTGAGAAGTGGGTCTTGCGGAGGGCATTCGACGACGAGCAGAATCCTTATCTTCCCAAGCACATTCTGTACAGACAGAAGGAGCAATTCAGCGATGGGGTTGGCTACAGCTGGATTGATGGCTTGAAATCACATGCGGCAGAGCATGTGACAGATACGATGCTGCGCAATGCCAAATACATATTCCCCCATAATACTCCAACTACGAAGGAGGCGTACTACTACAGGATGATATTTGAAAGATTCTATCCTCAGAACGCGGCACGGTTGACGGTCCCGGGCGGGCCAAGCGTGGCTTGTAGCACCGCAAAGGCAGTGGAATGGGATGCTGCGTGGGCTCACAATCTGGACCCCTCCGGCAGGGCTGCTCTAGGCGTCCATGCCTCCGCGTACGAAGAGTGTAACGCAGGTGCGAATGATGGCACCGGCAATGGGAACGTCGATACATTCAGCAAGATGCCAGAAAGGGAAGAGAGTGTAGGTGCCCGCGAAGTGCTGATCAAAGGTTGA___ATTGTAAAGTATTCGTATCGACTAGGGTAGCCACGTTACTATGTAATAATCTGTACACAGAAATGGTTGGGGAAGAGTATGTCTCTGTTGTACATCAAAGTGAGAGCTTGTGATTACTGTATTTCCATTGGCTGGTGTTAGTTTTCAAAGATTTCAATGTCGCAGCACTTTGTGATATTTAAAGAACGGTTTTATTTGGATGGGTTCAAAGTGAATGTGTTAAGTAAAAAATATGTATTTTGCTTTAAAAGTTATAGAAATTTTAGTTATATTTAGA

Fln Alignment:

Contig14147___MCGILAVFGCLDASQAKXXXXXXXXXXXXXXGPDWSGLYQSGHSFLAHQRLAIVDPASGDQPLYNEDRQIVVTVNGEIYNHEKLREQLKSHKFRTGSDCEIVAHLYEDYGEEFVNMxxDRRFSLVLLDTRDQSFIAARDAFGITPLYIGWGFDGSVWFASEMKALHDDCESFESFPPGHIYSSKQGGLRRWYNPPWYSENVIPSSPYDSLVLRRAFEKAVIKRLMTDVPFXXXXXXXXXXXXXAAVASRYLAATKAGKQ*GDKLHSFCVGLKGSPDLKAAKEVAEYLGTFHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFVNVAMDIDPEWKMVKPDIGRIEKWVLRRAFDDEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLRNAKYIFPHNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAAWAHNLDPSGRAALGVHASAYEECXXXXXXXXXXXXXXXFSKMPEREESVGAREVLIKG
E7EAQ2_______________MCGILAVFGCLDASQAKRVRVLELSRRLRHRGPDWSGLYQSGHSFLAHQRLAIVDPASGDQPLYNEDRQIVVTVNGEIYNHEKLREQLKSHKFRTGSDCEIVAHLYEDYGEEFVNMxxDGMFSFVLLDTRDQSFIAARDAFGITPLYIGWGFDGSVWFASEMKALHDDCESFESFPPGHIYSSKQGGLRRWYNPPWYSENVIPSSPYDSLVLRRAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVASRYLAATKAGKQWGDKLHSFCVGLKGSPDLKAAKEVAEYLGTFHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFVNVAMDIDPEWKMVKPDIGRIEKWVLRRAFDDEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLRNAKYIFPHNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAAWAHNLDPSGRAALGVHASAYEECNAGANDGTGNGNVDTFSKMPEREESVGAREVLIKG

SNPs (tot: 6)

Position	A %	C %	G %	Probability
1662	26		73	1.0
1786	87		12	0.999964
1919		14	85	0.999981
1993	22		77	0.999999
1998	88		11	0.923564
2110	33		66	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain