SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene13912

Length: 860 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v3.0_unigene13912 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Subtilisin-like serine protease n=5 Tax=Arabidopsis RepID=Q8LGA0_ARATH	-	-	0.0	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	52%
Sma3	Subtilisin-like protease	-	-	7.012e-40	-

Source	ECs	Term	Type	e value	Identity
Sma3	2-alkenal reductase.	EC:1.3.1.74	-	8.903e-07	-
Sma3	Cucumisin.	EC:3.4.21.25	-	0.0	-

Source	Gene names
Sma3	46C02.13; A43; AIR3; ARA12; ASP48; AT4g34980; At1g01900; At1g04110; At1g20160; At1g32960; At2g04160; At2g05920; At3g14067; At3g14240; At4g34980; At5g51750; At5g59810/mmn10_30; At5g67360; B1248C03.20; B1340F09.6; B1340F09.7; F20D22.12; F22M8.3; F9L11.13; G

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	external side of plasma membrane	GO:0009897	Cellular Component	-
Sma3	membrane	GO:0016020	Cellular Component	-
Sma3	extracellular matrix	GO:0031012	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	serine-type endopeptidase activity	GO:0004252	Molecular Function	-
Sma3	calcium ion binding	GO:0005509	Molecular Function	-
Sma3	identical protein binding	GO:0042802	Molecular Function	-
Sma3	proteolysis	GO:0006508	Biological Process	-
Sma3	response to auxin stimulus	GO:0009733	Biological Process	-
Sma3	plant-type cell wall modification	GO:0009827	Biological Process	-
Sma3	lateral root morphogenesis	GO:0010102	Biological Process	-
Sma3	stomatal complex morphogenesis	GO:0010103	Biological Process	-
Sma3	regulation of cell proliferation	GO:0042127	Biological Process	-
Sma3	negative regulation of catalytic activity	GO:0043086	Biological Process	-
Sma3	mucilage metabolic process involved seed coat development	GO:0048359	Biological Process	-
Sma3	mucilage extrusion from seed coat	GO:0080001	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Phosphofructokinase domain	IPR000023	-	-
Sma3	Ornithine/DAP/Arg decarboxylase	IPR000183	-	-
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	Major intrinsic protein	IPR000425	-	-
Sma3	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	-
Sma3	SEC7-like	IPR000904	-	-
Sma3	Protease-associated domain, PA	IPR003137	-	-
Sma3	Proteinase inhibitor I9	IPR010259	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	Peroxisome membrane protein, Pex16	IPR013919	-	-
Sma3	Peptidase S8, subtilisin-related	IPR015500	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Peptidase S10, serine carboxypeptidase, active site	IPR018202	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT3G14240.1	Subtilase family protein chr3:4741637-4743964 REVERSE LENGTH=775	53%
RefSeq	Arabidopsis thaliana	NP_566483.1	Subtilase family protein [Arabidopsis thaliana]	53%
RefSeq	Populus trichocarpa	XP_002331218.1	predicted protein [Populus trichocarpa]	53%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: C0PPS1

Fln msg: Separated hits, possible frame ERROR between 703 and 708, Distance to subject end: 366 aas, your sequence is shorter than subject: 287 - 690

Fln protein: SVFPTVGHWYRSTIATAVKAALKTESGSHMDALPLHVYNTVLHGFSARLTDDEAALMVKMPGVLAMFPDRVRQLHTTRSPGFLGLDTPNGSGLWADSDYGSDVVIGFLDSGIWPERRSFSDQGMAPVPKHWKGECESGSRFPKTLCNRKLVGARFSHEGYKAMYRILNETLEFRSARDSEGHGTHTASTAAGRYVEGANMMGYAQGIAKGIAPQARIAVYKVCWVYGCLDSDVxxxALDKAVGDGVDVLSLSIGGAAYRYDLDSFAIGTFEAVKRGVFVSASAGNDG

Protein Length: 288

Fln nts: GTCCGTGTTTCCGACGGTCGGGCACTGGTACAGATCAACAATTGCAACTGCTGTCAAGGCTGCCCTCAAAACTGAATCGGGCAGTCACATGGATGCTCTGCCGCTTCACGTTTATAACACGGTGTTGCATGGTTTCTCGGCAAGGTTGACAGATGACGAAGCCGCTCTGATGGTAAAAATGCCCGGCGTTCTCGCCATGTTCCCGGATCGTGTTCGCCAGCTGCACACGACGCGGTCCCCGGGCTTCCTCGGCCTTGACACGCCAAATGGAAGCGGCCTGTGGGCGGATTCGGATTACGGCTCCGATGTGGTGATCGGTTTCCTGGACTCCGGCATCTGGCCGGAAAGGCGGAGCTTCAGCGATCAGGGCATGGCTCCCGTCCCCAAGCACTGGAAAGGCGAATGCGAGAGTGGGAGCAGGTTTCCCAAGACCCTGTGTAATCGGAAGCTGGTGGGAGCGCGTTTTTCCCACGAAGGCTACAAAGCGATGTATCGTATCTTGAACGAGACCCTGGAATTCCGTTCAGCGCGGGATTCGGAAGGCCATGGCACGCACACCGCCTCCACCGCTGCAGGCCGATACGTCGAGGGAGCCAACATGATGGGTTACGCGCAGGGAATCGCCAAAGGAATAGCGCCCCAGGCACGTATTGCGGTCTATAAGGTCTGTTGGGTCTATGGCTGCCTTGATTCAGATGTACTxxxxxxTGCTCTGGACAAGGCAGTAGGTGACGGAGTAGACGTTCTTTCTCTGTCGATCGGAGGAGCTGCCTATCGTTATGATCTAGATAGCTTTGCGATCGGCACCTTCGAGGCGGTGAAGCGGGGGGTGTTCGTTTCTGCGTCAGCGGGTAACGACGGC

Fln Alignment:

Contig13912___FPTVGHWYRSTIATAVKAALKTESGSHMDALPLHVYNTVLHGFSARLTDDEAALMVKMPGVLAMFPDRVRQLHTTRSPGFLGLDTPNGSGLWAD-SDYGSDVVIGFLDSGIWPERRSFSDQGMAPVPKHWKGECESGSRFPKTLCNRKLVGARFSHEGYKAMYRILNETLEFRSARDSEGHGTHTASTAAGRYVEGANMMGYAQGIAKGIAPQARIAVYKVCWVYGCLDSDVLxxALDKAVGDGVDVLSLSIGGAAYRYDLDSFAIGTFEAVKRGVFVSASAGNDG
C0PPS1_______________FTSHEHWY----ASAVKSVLSEEEEPSI----LYNYDDAFHGFAARLNAAQAEALEKTHGILGIYPETVYELHTTRTPQFLGLETAE-SGMWPEKANFGHDVVIGVLDTGVWPESLSFNDRGMGPVPAHWKGACESGTNFTASHCNKKLIGARFLSRGYEAAVGPINETAEFRSPRDQDGHGTHTASTAAGAVVLKADLVGYAKGTARGMATRARIAAYKVCWVGGCFSTDILxxALDKAVADGVNVLSLSLGGGLEPYYRDSISLGTFGAMEKGIFVSCSAGNGG

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
716	33	66			0.997452
757	33		66		0.997452
759	33		66		0.997452
760			66	33	0.997452
761		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain