SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene11626

Length: 1132 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene11626

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	flavonoid 3'-hydroxylase [Glycine max]	-	-	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Sma3	Flavonoid 3'-hydroxylase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Flavonoid 3'-monooxygenase.	EC:1.14.13.21	-	1.665e-09	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Flavonoid biosynthesis	00941		1.665e-09	%
Sma3	Flavone and flavonol biosynthesis	00944		1.665e-09	%
Sma3	Biosynthesis of phenylpropanoids	01061		1.665e-09	%
Sma3	Metabolic pathways	01100		1.665e-09	%
Sma3	Biosynthesis of secondary metabolites	01110		1.665e-09	%
Sma3	Flavonoid 3',5'-hydroxylase.	EC:1.14.13.88	-	8.332e-14	-

Source	KEGGs	Term	e value	Identity
Sma3	Flavonoid biosynthesis	00941	8.332e-14	%
Sma3	Flavone and flavonol biosynthesis	00944	8.332e-14	%
Sma3	Biosynthesis of secondary metabolites	01110	8.332e-14	%

Source	Gene names
Sma3	5'H; 5'h; AT4g37360; AT4g37370; At1g66540; At4g37360; At4g37370; At5g07990; B1097H10.9; Bel; C3H3; CYP75; CYP75A1; CYP75A12\|F3'5'H1; CYP75A13; CYP75A3; CYP75B1; CYP75B2; CYP81B1c; CYP81B1l; CYP81B25; CYP81Q1; CYP81Q2; CYP82B1; CYP82C10v1; CYP82C11v1; CYP8

Source	GOs	Term	Type	Identity
Sma3	endoplasmic reticulum	GO:0005783	Cellular Component	-
Sma3	endoplasmic reticulum membrane	GO:0005789	Cellular Component	-
Sma3	microsome	GO:0005792	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	monooxygenase activity	GO:0004497	Molecular Function	-
Sma3	iron ion binding	GO:0005506	Molecular Function	-
Sma3	electron carrier activity	GO:0009055	Molecular Function	-
Sma3	flavonoid 3'-monooxygenase activity	GO:0016711	Molecular Function	-
Sma3	heme binding	GO:0020037	Molecular Function	-
Sma3	flavonoid biosynthetic process	GO:0009813	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome P450	IPR001128	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Cytochrome P450, E-class, group I	IPR002401	-	-
Sma3	Lipoxygenase, C-terminal	IPR013819	-	-
Sma3	Cytochrome P450, conserved site	IPR017972	-	-
Sma3		IPR017973	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Source	Species	ID	Description	e value	Identity
RefSeq	Arabidopsis thaliana	NP_001117558.1	putative cytochrome P450 [Arabidopsis thaliana]	0.0	46%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LLA5

Fln msg: your sequence is shorter than subject: 279 - 528

Fln protein: YIPFLRWLDLQGCERAMKKLHRRRDEFLQRVIDNSRLCMKKENDESLIDVLLHLVDKAEEFCSEDTIVKATAISMIGAATDTYANTTEWAIATLLQRSDVLKRAQQELDVIVGRERILEESDLPKLKYLEAIVKETLRLYPAAPLLLPHVAVAPCRVGGYYVPEGTEVLFNVWGTHRDPAVWESPLKFEPERFINSSIDVNGHDFTYIPFGYGRRACPGIWLAMRMLLLTVGRLLQGFDWSIPEGTEGVDMNEGRAITLHRAVPLEAAIRPRLPHHLY*

Protein Length: 280

Fln nts: TATATTCCATTCCTTAGATGGCTGGATCTCCAGGGCTGCGAGCGGGCAATGAAGAAGTTACACAGGCGACGGGATGAGTTTTTGCAGAGAGTGATAGATAACAGCCGCCTTTGTATGAAGAAGGAGAATGACGAGAGTCTGATAGACGTGCTTCTACATTTGGTGGACAAAGCCGAAGAGTTCTGCAGTGAAGACACCATTGTTAAGGCCACTGCTATAAGCATGATCGGCGCAGCAACGGATACCTACGCCAACACAACGGAATGGGCTATCGCAACGCTCCTGCAACGCTCGGATGTGCTGAAAAGGGCACAGCAGGAGCTGGACGTCATAGTTGGAAGGGAGCGCATTTTGGAGGAATCGGATCTCCCGAAGTTGAAGTATTTGGAGGCCATCGTGAAAGAGACTCTACGTCTCTATCCAGCGGCGCCTCTGCTCCTCCCTCATGTTGCAGTGGCGCCGTGCAGAGTGGGAGGGTATTATGTACCAGAGGGCACAGAGGTTTTGTTCAATGTGTGGGGTACACACAGAGACCCAGCTGTGTGGGAAAGCCCTCTAAAATTCGAGCCGGAGAGGTTTATCAACAGTTCCATAGACGTGAACGGACATGATTTCACATATATACCGTTTGGTTATGGGAGAAGGGCCTGCCCGGGCATTTGGTTAGCGATGCGCATGCTTCTGCTCACCGTGGGAAGACTGTTGCAGGGTTTCGATTGGAGCATTCCCGAAGGAACTGAAGGAGTTGATATGAATGAGGGTCGTGCTATTACGCTGCACAGAGCTGTTCCATTGGAGGCGGCCATCAGGCCTCGTCTTCCTCACCATCTGTATTGA___GTCATCACCTCACCTGATCTGTGATGTTGATGAAGTGAGAAACATAGGGGGCTCGGATGATTGACTACTCCAAAAGTTCTATAGCATGGGATTTGTAAACCTCGGATTGCAACGTGAGCAGTGTACGAGATGTGGTTGAAGCATATTTTATATGTAAGACATTATAGTTGGTAGCCTGGTACAATCCCTAATGCTCTTTTCTCTTCTCCTCTTTTTTTAAGGAAAGGAATGAATTAATTTCTTAAGGATGATGTGGAGTCATTAAAGATGATGAATTTCAATGATCTTCTACATT

Fln Alignment:

Contig11626___YIPFLRWLDLQGCERAMKKLHRRRDEFLQRVIDNSRLCMKKENDESLIDVLLHLVDKAEEFCSEDTIVKATAISMIGAATDTYANTTEWAIATLLQRSDVLKRAQQELDVIVGRERILEESDLPKLKYLEAIVKETLRXXXXXXXXXXXXXXXXCRVGGYYVPEGTEVLFNVWGTHRDPAVWESPLKFEPERFINSSI-DVNGHDFTYIPFGYGRRACPGIWLAMRMLLLTVGRLLQGFDWSIPEGTEGVDMNEGRAITLHRAVPLEAAIRPRLPHHLY
B8LLA5_______________YIPFLRWLDLQGCERAMKKLNRRRDKFLQRAIDNSRLCIKEENDESLIDVLIHLVDKAEDFFSDEAIVKATAISIIIGATDTYANTTEWALATLLQRPEVLKRAQEELDVVVGSERVLEESDLPNLKYLEAIVKETLRLYPAGPLLLPHMAAAPCIVGGYYVPAGTELLLNAWGIHRDPAVWERPLEFEPERFLNSSSPDLNGHDFKYIPFGYGRRACPGMWVALRMLLLTVGRLLQSFDWSIPDGIEGVDMNEGRALTLHKAVPLEAAIKPRLPQHLY

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
462	12		87		0.992554
465		87	12		0.992554
514	10		90		0.90298
543	10		90		0.90298
825		90		10	0.90298
856	20	80			0.999996
866	12		87		0.992554
872			83	16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain