SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene10612

Length: 1464 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene10612

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glutamine synthetase cytosolic isozyme n=2 Tax=Pinaceae RepID=GLNA_PINSY	-	-	92%
FL-Next	sp=Glutamine synthetase cytosolic isozyme; Pinus sylvestris (Scots pine).	-	-	99%
Sma3	Glutamine synthetase	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Glutamate--ammonia ligase.	EC:6.3.1.2	-	0.0	-

Source	KEGGs	Term	Identity
Sma3	Alanine, aspartate and glutamate metabolism	00250	%
Sma3	Arginine and proline metabolism	00330	%
Sma3	Nitrogen metabolism	00910	%
Sma3	Metabolic pathways	01100	%

Source	Gene names
Sma3	AT1G66200; AT5G35630; At1g48470; At1g66200; At3g17820; At5g16570; At5g35630; At5g37600; CHLREDRAFT_129468; CHLREDRAFT_133971; CHLREDRAFT_136895; CsGS1; DtCGS-1; F15E12.14; GLN1; GLN1-1; GLN1-2; GLN1-3; GLN1-4; GLN1-5; GLN2; GLN3; GLN4; GLN5; GLN6; GLNA; G

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	chloroplast thylakoid membrane	GO:0009535	Cellular Component	-
Sma3	chloroplast stroma	GO:0009570	Cellular Component	-
Sma3	chloroplast envelope	GO:0009941	Cellular Component	-
Sma3	cytosolic ribosome	GO:0022626	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	glutamate-ammonia ligase activity	GO:0004356	Molecular Function	-
Sma3	copper ion binding	GO:0005507	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	glutamine biosynthetic process	GO:0006542	Biological Process	-
Sma3	nitrogen compound metabolic process	GO:0006807	Biological Process	-
Sma3	aging	GO:0007568	Biological Process	-
Sma3	nitrogen fixation	GO:0009399	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	ammonia assimilation cycle	GO:0019676	Biological Process	-
Sma3	nitrate assimilation	GO:0042128	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Glutamine synthetase, catalytic domain	IPR008146	-	-
Sma3	Glutamine synthetase, beta-Grasp	IPR008147	-	-
Sma3	Glutamine synthetase/guanido kinase, catalytic domain	IPR014746	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G16570.1	" GLN1;4 glutamine synthetase 1;4 chr5:5421898-5424523 REVERSE LENGTH=356"	81%
RefSeq	Arabidopsis thaliana	NP_568335.1	glutamine synthetase cytosolic isozyme 1-4 [Arabidopsis thaliana]	81%
RefSeq	Populus trichocarpa	XP_002305885.1	predicted protein [Populus trichocarpa]	85%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: P52783

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 286 - 357

Fln protein: GSPQAIFRDPFRRGKHILVICDAYSPNGTAIPSNKRAAAAKIFNEKAVIDEETWYGLEQEYTLLQKDVKWPLGWPIGGYPGPQGPYYCGVGADKAWGRDMVDAHYKACLYSGINISGINGEVMPGQWEFQVGPSVGISAADELWCARFIMERITEKAGVVLSFDPKPIEGDWNGAGCHTNYSTKSMRKEGGFEVIKKAIEKLKLRHKEHISAYGEGNERRLTGRHETADMNTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYIVTSMIAETTILWKP*

Protein Length: 287

Fln nts: GTTTTTACTGTTCTCTTCCTTTCATCATCGTGCATAGGCCTGGGTATATGGTCCTTTTGTTTTATTAATTTGATTCTGTACGGCCCAAAATGCAACATAAATTAACAGTTTAGAATATTCTTCCCTGTACTCTGAAATTTCTAAGATATTACTTGATTCCAAGTCTATATGTGTTTCCAATATTAAATGTTCTTGTTCTCATAGTTTGGAACATATATAACCCAATTACGTGGTTTGTTCTGATTGAGGCAGTCCACAAGCTATCTTCCGTGATCCATTTCGCCGAGGAAAGCACATTTTGGTAATCTGTGATGCCTACTCTCCTAATGGGACTGCTATTCCTTCCAACAAGAGGGCTGCAGCAGCGAAAATTTTTAACGAAAAGGCGGTTATTGATGAAGAGACATGGTACGGGCTTGAACAAGAATATACACTGTTGCAAAAGGACGTCAAATGGCCTCTTGGCTGGCCAATTGGTGGCTACCCCGGTCCTCAGGGCCCATATTACTGTGGAGTTGGAGCTGACAAAGCCTGGGGACGTGACATGGTTGATGCCCATTATAAGGCTTGTCTCTATTCAGGAATCAATATCAGTGGCATCAATGGAGAAGTCATGCCAGGGCAGTGGGAATTTCAAGTAGGTCCGTCAGTGGGTATCTCAGCAGCAGATGAGCTGTGGTGTGCTCGTTTTATTATGGAGAGGATTACAGAAAAGGCGGGTGTCGTTCTGTCCTTTGATCCCAAGCCAATTGAGGGGGACTGGAATGGTGCTGGATGCCACACAAATTACAGCACCAAGTCCATGCGCAAGGAGGGAGGCTTCGAAGTAATTAAGAAAGCAATAGAAAAACTGAAGTTGAGGCATAAGGAGCATATTTCTGCCTATGGAGAGGGCAATGAGAGACGCCTCACTGGTCGGCACGAGACAGCAGACATGAATACCTTTTCCTGGGGTGTTGCAAATCGAGGAGCTTCAGTTAGAGTGGGCCGGGACACAGAAAAAGAAGGAAAAGGTTATTTTGAGGACCGTCGACCTGCTTCAAACATGGATCCATACATAGTGACTTCTATGATTGCTGAGACGACCATTCTATGGAAACCTTAA___ATTACAAAGTGGAGGCCAGTTACACGCGTGGTCGTGGTGCTTTGCTTTGGAGGCCAGCGTCACTGATAAGCTATATGTATGTAAATGTGATGCCAATGTTTAAGTAGGGTGGTAACTTTGCTTTGGTTGTGGGTAGACCTGAACTTTGGTCAAACAATTTCCTTTTGCGAGGGCTTCGAAAGACTCTTTTACCTTTGTTTCTTCCGTTGTGGAAGATGTATTCGACAAATTGTTTAGTATGTTTGAATATGATGATATTTTCTTTGTATTGAAAAAGAATCAGTGACTATTCAAGAACATGGAAGGAAAAGAAGGCATAACCATTACTCCCAGCTCTTACAAATCGAAGGGCATTAGAG

Fln Alignment:

Contig10612___PQAIFRDPFRRGKHILVICDAYSPNGTAIPSNKRAAAAKIFNEKAVIDEETWYGLEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDMVDAHYKACLYSGINISGINGEVMPGQWEFQVGPSVGISAADELWCARFIMERITEKAGVVLSFDPKPIEGDWNGAGCHTNYSTKSMRKEGGFEVIKKAIEKLKLRHKEHISAYGEGNERRLTGRHETADMNTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYIVTSMIAETTILWKP
P52783_______________PQAIFRDPFRRGKHILVICDAYSPNGTAIPSNKRAAAAKIFNEKAVSDEETWYGLEQEYTLLQKDVKWPLGWPIGGYPGPQGPYYCGVGADKAWGRDIVDAHYKACLYSGINISGINGEVMPGQWEFQVGPSVGISAADELWCARFIMERITEKAGVVLSFDPKPIEGDWNGAGCHTNYSTKSMRKEGGFEVIKKAIEKLKLRHKEHISAYGEGNERRLTGRHETADMNTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYIVTSMIAETTILWKP

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
2			50	50	1.0
107	85		14		0.993533
1234	3		96		0.988939
1266	4	95			0.996252
1267	4	95			0.996252

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain