SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene10329

Length: 2520 nt

UniGene Fasta

>sp_v3.0_unigene10329
GACGTTGGTGACTTTTGAGGCCTGTATAGCTTTCTCAGTAAAGCGCAAATCATGGACTCCCATATTCATTGGATCCATAATCATTGAAGTGTTGGTGATCGTGGGACATATGAAACTCTGCATTTACTGCTCCTTCCGACCTCTCTCTTCTTCGTTCCACTGTTCTGAATTTGTTAGACACACACCTTTTGGGATCAAGAAAGGAGTTCAGTTCAGTTTTGCTGTAGCCAGAGCTCGCGATCCTTGCATTTGAGTGGAATTATGGCCCAGTTAATAAGAACAAGGAGAAACTTTTCAACTGCCACTGGAAGGTTGCAGACATGGCTTCTGCCTCCTTTCTTCACTGATGATGCTGCTGGTCGCATTAGCAGGCGGCCTATGATGACTGAGGCCACTCGCCCCACTATTGTCCACAAGCGCGGCCTCGACATTCTCCACGATCCCTGGTTTAACAAGGGCACAGCTTTCTCAATGACAGAGCGAGACCGGCTTGATCTACGTGGCCTTCTGCCACCAAATGTCATGAGTTGGAAGCAACAAGTTGATCGCTTTATGGTTGATCTGAAAAGACTGGAAGAACATGCTAAAGATGGGCCATCTGACACTACTGCTTTAGCTAAGTGGAGAATTCTGAATAGATTGCATGACAGGAATGAGACAATGTATTACAAGGTTTTAATTGAAAATATTGCAGAGTATGCGCCAATAGTATACACACCTACCGTTGGTATGGTCTGTCAAAACTACAGTGGTTTGTTTAGACGGCCGAGAGGAATGTATTTTAGCACTGCTGATCGGGGGGAAATGATGTCAATGGTCTATAACTGGCCTGCAGAACAGGTTGACATGATTGTGGTTACGGACGGAAGTAGAATATTGGGACTTGGAGATCTTGGAGTTCAGGGCATTGGTATTGCAATTGGAAAGTTAGACTTATATGTTGCAGCTGCAGGCATAAATCCTCAAAGGGTGCTTCCTGTGATGATTGATTGTGGGACAAATAATGAGAAGCTCCTTGGAGATCCTTTATATCTAGGATTGCCAATACATAGACTTGATGGGGAAGAGTATGTTTCAGTTATTGATGAATTCATGGAAGCAGTTTTTACAAGGTGGCCACATGTCATAGTCCAGTTTGAGGACTTCCAAAGCAAGTGGGCATTCAAATTGCTACAGCGTTATCGAAATCATTATAGAATGTTTAATGATGATGTTCAGGGAACTGCGGGAGTTGCTATTGCAGGTCTTCTAGGAGCTGTAAGAGCACAAGGCCGACCAATATCAGACTTTGCAAAGCAAAAGATTGTTGTTGCTGGTGCAGGAAGTGCAGGAATAGGAGTCCTGAATGCTGCCAGTAAAGCAATGGCGAGATTTTTAGGTGGTACAGAATCTGCCTTGGAACGTGCAAGGTACCAGTTCTGGGTAGTTGATGCAATGGGTTTGGTTACAGAAGCACGGAATGACATTGATGTTGATGTTGCACCTTTTGCTAGAAAAGTTGAGGAACTAGGTTCGCAAGATTTAAGAGAAGGTTCCAATCTTACTGAAGTGGTAAGAAAAGTTAGGCCTGATGTGTTGATTGGATTATCTGCAGTTGGAGGATTATTTACGGAAGAGGTTTTGGAAGCCCTTAAAGAATCAACTTGTGCCAGGCCAGCAGTTTTTGCAATGTCAAATCCAACTACGAATGCCGAATGTACACCCGAGCAAGCTTTCAACATTGTGGGACACCAAATAGTTTTTGCAAGTGGAAGTCCATTTCCTGACGTGGATCTTGGAAATGGCCAGATTGGACATTCCAACCAAGGCAACAATATGTACCTTTTCCCAGGGATTGGGCTGGGTACACTTCTCTCTGGTGCTCGTATCATTTCAGATGGCATGTTACAAGCTGCAGCAGAATGCCTAGCTGAATGCATGACAGAGGAAGAAGTGCAAAGGGGGATTATATATCCCTCAACATCCAGGATTCGTGAGATTACAATGGAGGTAGCTGCATCTGTTGTGAGAGAAGCAGTGGCAGAGGAGCTTGTAGAGGGCTATCGGGAGACAGATGCTAGAGAGCTTCGACGTATGTCAAAGGAAGAGACTTTGGAGCATGTGGAAAAGCATATGTGGGTTCCAAACTATGCACCATTGGTTTACCATAAGCAATAAGCTATGGAATAAAGGCAGCAATAGTCAGTGGCTTTGCATATTCGTTCCCAAGTTGCAACTATGAAATGAAAATTGTCAGACACAATATATATCTCAATAGACTTCGAGTAGCAGGGTAGGATTCCTGGCATCTTATTTTGCATAAGAATCAGAGTTATGACATGAATGTCTCAAGTTGCAACGAAAGAATAATGCGCATTACCTGTATTTGTTATAAATCCTCAGTTATGTCGATAACTTGGCTTCTCTCTCCACTGTAAGCATTACTGTGCTTTCTATTTTAGGGCGAAATTCTTTAGTATGCTAGTTCATTTAATAAATAGTTTTTTCCTTGTAGGCAATTCTTTTCACCGTCTTCACATT

Ace file of the UniGene sp_v3.0_unigene10329

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Malic enzyme n=1 Tax=Picea sitchensis RepID=B8LMR7_PICSI	-	-	81%
FL-Next	sp=Malic enzyme; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Sma3	Malic enzyme	-	-	-

Source	ECs	Term	Type	e value	Identity
Sma3	Malate dehydrogenase (decarboxylating).	EC:1.1.1.39	-	5.051e-16	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Pyruvate metabolism	00620		5.051e-16	%
Sma3	Carbon fixation in photosynthetic organisms	00710		5.051e-16	%
Sma3	Biosynthesis of terpenoids and steroids	01062		5.051e-16	%
Sma3	Biosynthesis of alkaloids derived from shikimate pathway	01063		5.051e-16	%
Sma3	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064		5.051e-16	%
Sma3	Biosynthesis of alkaloids derived from histidine and purine	01065		5.051e-16	%
Sma3	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066		5.051e-16	%
Sma3	Metabolic pathways	01100		5.051e-16	%
Sma3		EC:1.1.1.4-	-	5.196e-32	-

Source	Gene names
Sma3	AT4g00570; AT5G11670; At1g79750; At2g13560; At2g19900; At2g19900/F6F22.7; At4g00570; At5g11670; At5g25880; CHLREDRAFT_147722; CHLREDRAFT_196831; CHLREDRAFT_196832; CHLREDRAFT_196833; CytMeA; CytMeB; F19K16.27; F6N23.16; GSVIVT00016671001; GSVIVT0001898400

Source	GOs	Term	Type	Identity
Sma3	cell wall	GO:0005618	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	mitochondrial matrix	GO:0005759	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	apoplast	GO:0048046	Cellular Component	-
Sma3	malic enzyme activity	GO:0004470	Molecular Function	-
Sma3	malate dehydrogenase (decarboxylating) activity	GO:0004471	Molecular Function	-
Sma3	malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity	GO:0004473	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	zinc ion binding	GO:0008270	Molecular Function	-
Sma3	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	GO:0016616	Molecular Function	-
Sma3	metal ion binding	GO:0046872	Molecular Function	-
Sma3	NAD binding	GO:0051287	Molecular Function	-
Sma3	malate metabolic process	GO:0006108	Biological Process	-
Sma3	fatty acid biosynthetic process	GO:0006633	Biological Process	-
Sma3	response to stress	GO:0006950	Biological Process	-
Sma3	metabolic process	GO:0008152	Biological Process	-
Sma3	pentose-phosphate shunt, oxidative branch	GO:0009051	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	response to cadmium ion	GO:0046686	Biological Process	-
Sma3	oxidation-reduction process	GO:0055114	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Malic oxidoreductase	IPR001891	-	-
Sma3	Aminotransferase, class-II, pyridoxal-phosphate binding site	IPR001917	-	-
Sma3	FAR1 DNA binding domain	IPR004330	-	-
Sma3	Zinc finger, PMZ-type	IPR006564	-	-
Sma3	Zinc finger, SWIM-type	IPR007527	-	-
Sma3	Malic enzyme, N-terminal	IPR012301	-	-
Sma3	Malic enzyme, NAD-binding	IPR012302	-	-
Sma3	Malic enzyme, conserved site	IPR015884	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	MULE transposase domain	IPR018289	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT2G13560.1	NAD-ME1 NAD-dependent malic enzyme 1 chr2:5650089-5655103 FORWARD LENGTH=623	77%
RefSeq	Arabidopsis thaliana	NP_178980.1	NAD-dependent malic enzyme 1 [Arabidopsis thaliana]	77%
RefSeq	Populus trichocarpa	XP_002302483.1	predicted protein [Populus trichocarpa]	81%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LMR7

Fln msg: your sequence is longer than subject: 632 - 601

Fln protein: MAQLIRTRRNFSTATGRLQTWLLPPFFTDDAAGRISRRPMMTEATRPTIVHKRGLDILHDPWFNKGTAFSMTERDRLDLRGLLPPNVMSWKQQVDRFMVDLKRLEEHAKDGPSDTTALAKWRILNRLHDRNETMYYKVLIENIAEYAPIVYTPTVGMVCQNYSGLFRRPRGMYFSTADRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAAAGINPQRVLPVMIDCGTNNEKLLGDPLYLGLPIHRLDGEEYVSVIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNHYRMFNDDVQGTAGVAIAGLLGAVRAQGRPISDFAKQKIVVAGAGSAGIGVLNAASKAMARFLGGTESALERARYQFWVVDAMGLVTEARNDIDVDVAPFARKVEELGSQDLREGSNLTEVVRKVRPDVLIGLSAVGGLFTEEVLEALKESTCARPAVFAMSNPTTNAECTPEQAFNIVGHQIVFASGSPFPDVDLGNGQIGHSNQGNNMYLFPGIGLGTLLSGARIISDGMLQAAAECLAECMTEEEVQRGIIYPSTSRIREITMEVAASVVREAVAEELVEGYRETDARELRRMSKEETLEHVEKHMWVPNYAPLVYHKQ*

Protein Length: 633

Fln nts: GACGTTGGTGACTTTTGAGGCCTGTATAGCTTTCTCAGTAAAGCGCAAATCATGGACTCCCATATTCATTGGATCCATAATCATTGAAGTGTTGGTGATCGTGGGACATATGAAACTCTGCATTTACTGCTCCTTCCGACCTCTCTCTTCTTCGTTCCACTGTTCTGAATTTGTTAGACACACACCTTTTGGGATCAAGAAAGGAGTTCAGTTCAGTTTTGCTGTAGCCAGAGCTCGCGATCCTTGCATTTGAGTGGAATT_-_ATGGCCCAGTTAATAAGAACAAGGAGAAACTTTTCAACTGCCACTGGAAGGTTGCAGACATGGCTTCTGCCTCCTTTCTTCACTGATGATGCTGCTGGTCGCATTAGCAGGCGGCCTATGATGACTGAGGCCACTCGCCCCACTATTGTCCACAAGCGCGGCCTCGACATTCTCCACGATCCCTGGTTTAACAAGGGCACAGCTTTCTCAATGACAGAGCGAGACCGGCTTGATCTACGTGGCCTTCTGCCACCAAATGTCATGAGTTGGAAGCAACAAGTTGATCGCTTTATGGTTGATCTGAAAAGACTGGAAGAACATGCTAAAGATGGGCCATCTGACACTACTGCTTTAGCTAAGTGGAGAATTCTGAATAGATTGCATGACAGGAATGAGACAATGTATTACAAGGTTTTAATTGAAAATATTGCAGAGTATGCGCCAATAGTATACACACCTACCGTTGGTATGGTCTGTCAAAACTACAGTGGTTTGTTTAGACGGCCGAGAGGAATGTATTTTAGCACTGCTGATCGGGGGGAAATGATGTCAATGGTCTATAACTGGCCTGCAGAACAGGTTGACATGATTGTGGTTACGGACGGAAGTAGAATATTGGGACTTGGAGATCTTGGAGTTCAGGGCATTGGTATTGCAATTGGAAAGTTAGACTTATATGTTGCAGCTGCAGGCATAAATCCTCAAAGGGTGCTTCCTGTGATGATTGATTGTGGGACAAATAATGAGAAGCTCCTTGGAGATCCTTTATATCTAGGATTGCCAATACATAGACTTGATGGGGAAGAGTATGTTTCAGTTATTGATGAATTCATGGAAGCAGTTTTTACAAGGTGGCCACATGTCATAGTCCAGTTTGAGGACTTCCAAAGCAAGTGGGCATTCAAATTGCTACAGCGTTATCGAAATCATTATAGAATGTTTAATGATGATGTTCAGGGAACTGCGGGAGTTGCTATTGCAGGTCTTCTAGGAGCTGTAAGAGCACAAGGCCGACCAATATCAGACTTTGCAAAGCAAAAGATTGTTGTTGCTGGTGCAGGAAGTGCAGGAATAGGAGTCCTGAATGCTGCCAGTAAAGCAATGGCGAGATTTTTAGGTGGTACAGAATCTGCCTTGGAACGTGCAAGGTACCAGTTCTGGGTAGTTGATGCAATGGGTTTGGTTACAGAAGCACGGAATGACATTGATGTTGATGTTGCACCTTTTGCTAGAAAAGTTGAGGAACTAGGTTCGCAAGATTTAAGAGAAGGTTCCAATCTTACTGAAGTGGTAAGAAAAGTTAGGCCTGATGTGTTGATTGGATTATCTGCAGTTGGAGGATTATTTACGGAAGAGGTTTTGGAAGCCCTTAAAGAATCAACTTGTGCCAGGCCAGCAGTTTTTGCAATGTCAAATCCAACTACGAATGCCGAATGTACACCCGAGCAAGCTTTCAACATTGTGGGACACCAAATAGTTTTTGCAAGTGGAAGTCCATTTCCTGACGTGGATCTTGGAAATGGCCAGATTGGACATTCCAACCAAGGCAACAATATGTACCTTTTCCCAGGGATTGGGCTGGGTACACTTCTCTCTGGTGCTCGTATCATTTCAGATGGCATGTTACAAGCTGCAGCAGAATGCCTAGCTGAATGCATGACAGAGGAAGAAGTGCAAAGGGGGATTATATATCCCTCAACATCCAGGATTCGTGAGATTACAATGGAGGTAGCTGCATCTGTTGTGAGAGAAGCAGTGGCAGAGGAGCTTGTAGAGGGCTATCGGGAGACAGATGCTAGAGAGCTTCGACGTATGTCAAAGGAAGAGACTTTGGAGCATGTGGAAAAGCATATGTGGGTTCCAAACTATGCACCATTGGTTTACCATAAGCAATAA___GCTATGGAATAAAGGCAGCAATAGTCAGTGGCTTTGCATATTCGTTCCCAAGTTGCAACTATGAAATGAAAATTGTCAGACACAATATATATCTCAATAGACTTCGAGTAGCAGGGTAGGATTCCTGGCATCTTATTTTGCATAAGAATCAGAGTTATGACATGAATGTCTCAAGTTGCAACGAAAGAATAATGCGCATTACCTGTATTTGTTATAAATCCTCAGTTATGTCGATAACTTGGCTTCTCTCTCCACTGTAAGCATTACTGTGCTTTCTATTTTAGGGCGAAATTCTTTAGTATGCTAGTTCATTTAATAAATAGTTTTTTCCTTGTAGGCAATTCTTTTCACCGTCTTCACATT

Fln Alignment:

Contig10329___MMTEATRPTIVHKRGLDILHDPWFNKGTAFSMTEXXXXXXXXXXPPNVMSWKQQVDRFMVDLKRLEEHAKDGPSDTTALAKWRILNRLHDRNETMYYKVLIENIAEYAPIVYTPTVGMVCQNYSGLFRRPRGMYFSTADRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAAAGINPQRVLPVMIDCGTNNEKLLGDPLYLGLPIHRLDGEEYVSVIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNHYRMFNDDVQXXXXXXXXXXXXXXRAQGRPISDFAKQKXXXXXXXXXXXXXLNAASKAMARFLGGTESALERARYQFWVVDAMGLVTEARNDIDVDVAPFARKVEELGSQDLREGSNLTEVVRKVRPDVLIGLSAVGGLFTEEVLEALKESTCARPAVFAMSNPTTNAECTPEQAFNIVGHQIVFASGSPFPDVDLGNGQIGHSNQGNNMYLFPGIGLGTLLSGARIISDGMLQAAAECLAECMTEEEVQRGIIYPSTSRIREITMXXXXXXXXXXXXXXXXXGYRETDARELRRMSKEETLEHVEKHMWVPNYAPLVYH
B8LMR7_______________VITEATRPTIVHRRGLDILHDPLFNKGTAFPLAERDRLGLRGLLPPSVMTWKQQIDRFMVDLKRLEEHAKDGPSDTTALAKWRILNRLHDRNETLYYKVLIDNIAEYAQIIYTPTVGLVCQNYSGLYRRPRGMYFSTADQGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVQGIGIAIGKLDLYVAASGINPQRVLPVMIDCGTNNEKLLGDPLYLGLPIRRLDGEEYVSVIDEFMEAVFARWPNVIVQFEDFQSKWAFKLLQRYRNQYRMFNDDVQGTAGVAIAGLLGAVRAQGRPISDFAKQKIVVSGAGSAGIGVLNAARKAMARFLGGTESAFENARCQFWVLDAMGLVTEARNDIDPDVAPFARKVEELGSQGLREGSQLTEVVKKVRPDVLIGLTGIGGLFTKEVLEALKESTCVRPVVFAMSNPTSNAECTADQAFNTVGHHIVFASGSLFPDVDLGNGQIGHSNQGNNMYLFPGIGLGTLVSGARLISDGMLQAAAECLAECMTKEEVRKGIIYPSVSRIRNITMKVATAVVREAVAEDLAEGYQDKDARELQRLSKEETLEHVEKHMWVPNYSPLVYH

SNPs (tot: 50)

Position	A %	C %	G %	T %	Probability
56	80		20		0.995493
57		80	20		0.995493
58			20	80	0.995493
59		80		20	0.995493
60		80		20	0.995493
61		80		20	0.995493
66		20		80	0.995493
68	80		20		0.995493
70			16	83	0.994512
73	66		33		1.0
74	33			66	1.0
79	66			33	1.0
80	66		33		1.0
81			33	66	1.0
84			33	66	1.0
88	66			33	1.0
89	33		66		1.0
127		50	50		1.0
149		83	16		0.994512
176		42		57	1.0
195		42	57		1.0
205	42		57		1.0
625	33		66		0.997452
630			66	33	0.997452
668	25			75	0.996473
671			25	75	0.996473
672	75			25	0.996473
736			80	20	0.995493
1036	33			66	0.997452
1037	33	66			0.997452
1402	14		85		0.993533
1403		14	85		0.993533
1405	85			14	0.993533
1406		85		14	0.993533
1432			11	88	0.923564
1735	18	81			0.999994
1774	12		87		0.992554
1846			75	25	0.996473
1899	16	83			0.994512
1901			83	16	0.994512
1902		83	16		0.994512
1903	83			16	0.994512
1998	10	90			0.90298
2001		90	10		0.90298
2302		88		11	0.923564
2315	11		88		0.923564
2321	88		11		0.923564
2348			87	12	0.992554
2356	14	85			0.993533
2458			22	77	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain