SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene10249

Length: 2187 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v3.0_unigene10249
ATGGGACTTCACTTCTATAAAATGGGAGGTCCATGTGAACTCAGAGAGAAAACCCTTGTCTTCATGGTTTTATTTATTTTCCTGCATAAGAGCTTCATTAATCTTTTCTACCTTTAGAGAGCAACTCCATTAACCTGTTGTCTTTCTTTGCCTTCCTTACAGAACAACTCCATTACTTTTGAGTTGCAGCTGATATCGTCCTCTACCTTTTCCCACAAAAGCAATCTGTATATACAGAGAATGCTGTAAGATGCCCCTGCAGGACGCCCAGGCCATCAGCGGCCGCACTCAAGCACAGCTCGCGTGCAAAGAAAAGAGGCAGAAGATGAGCAGAAACACTCACGACGTCTGGAATGGAGTGCATATCGGAGAACAGGGCCTCGGCAGAGAAATCGCATAAACCCAACATATGCAAGAGAGCTAAGCAGCAGCAGGCGGATTTGTGACATTTTCTTTTGAAGTAATGGAGGTTAAGCACAATATTCTGATGGGGAGGTATGAATTAGGGCGCCTGTTGGGGCAAGGGACGTTCGCCAAGGTCTACTACGCCAGAAGTCTGAAGACAGGCGAGAGCGTGGCCATTAAAGCCATGGACAAGGAAAAAATCCTCAAGGTCGGCATGATCGAGCAGATTAAACGGGAAATCTCGGTTATGAGACTGGTTCGGCATCCCAACATCGTTCGGCTCTACGAAGTCATGGCGAGCAAGTCCAAGATTTACTTCGTCATGGAGTATGTTAAGGGCGGTGAGCTCTTCAACAGGGTTGTTGAAAATGATATACTGGAAGAGGAGGAGGCGAAGAAGTATTTTCAAGAATTGATTAGCGCGGTGGATTTCTGCCACAGCCGGGGTGTGTACCACCGGGATTTGAAGCTGGAGAACTTGTTATTAGACGAGAAAGGGGTCGTGAAGGTCTCGGACTTCGGCCTCAGCGCATTGCCGGCGCAATTCCGGCCGGACGGCCTTCTCCACACGGCCTGCGGCACGCCGGCTTATGTCGCACCGGAGGTCATTACCAGAAAGGGTTACGATGGCGCTAAAGCGGACATCTGGTCCTGCGGGGTTATACTGTTCGTGCTCCTGGCCGGCTACCTGCCGTTCCAGGGCGCGAATCTGATGGTGATGTATAAGAAGATCTACAAAGGGGACTTCAAGTGCCCGACCTGGTTTTCCTGGGAAGCACGGCGACTTGTCCTCCGGCTTCTGGACCCCGATCCGCAGACGAGGATCACCATTCCACAGCTGATGGAGGTCCCCTGGTTCCGCCGGGGTTTCAAACGCCCGCAAGGCGACACTGACATCAGTGTCGGACTCCTCGACGATGTCGACTCGGCCTTTTCCGGCCCGAATCCGGCCTGCCTGAACGCGTTCGACATAATCTCCCTCTCGCCGGGGTTCAATCTCTCCGGCCTATTCAAGGATGATGTCTTGAAGCAGGAGAAGGAATCGAGGTTCACATCGACTCAGCCGGCGTCAAGCATAATGTCGAAGCTGGAGGAGATTGCCAGTCTGTTTCGGTTCAGCTTTAAGAAATCGAAGGAATTCAAGGTGAAGCTGAAGGGATCGGAGCAGGGGCGGAAGGGGCATTTGTCGATCGATGCCGAGATTTTCGAAGTCACGCCCTCGCTGTTCGTGGTAGAGCTTCGGAAGACCGGCGGGGATACACTGGAATACAGTAAGTTCTGCAGCCAAGATCTGAGGCCCGGCCTTAAAGACATCGTGTTGGCATGGGACGGCAACAGACCAGAGAAGTAATAGCATATGTTCACCGTATATACGTCGTGATGGTTGCTCAGTTTTAAACAGTTATATTCACTGTATATACGTCGTGTTGGTTGTTTAGTTTTAAATGGTGTTTGTTTTACCAGACCGACGTTCAGTCCTCCTCGATCCAGAGAAAGCTCTCCTAATAGCATATATTCACTGTATATACGTGGTGATGGTTGCTTAGATTTAAATGGTTATGTTCACTGTATATACGTCGTGGTGTTTGCTTTGTTTTATAGGGTTTTCGTTTTACCAGACCGACGGCCAGGCCTCCTCGATCTAAAGAAAGCTCTATTCCGTACTTGGGCGGCGGTCCCCCTCCCGCGTCAATGTATGTATAAATACATCTGTCTCGATGTGAGCGGTTTTAATGGGTGAGAATGTGGATTTAATGTTATTTTATATTTTAAAGAAATAAA

Ace file of the UniGene sp_v3.0_unigene10249 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	CBL-interacting protein kinase 16 n=2 Tax=Vitis vinifera RepID=C4P7W5_VITVI	-	-	0.0	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	88%
Sma3	CBL-interacting serine/threonine-protein kinase, putative	-	-	7.779e-38	-

Source	ECs	Term	Type	e value	Identity
Sma3	Non-specific serine/threonine protein kinase.	EC:2.7.11.1	-	0.0	-
Sma3	Calcium/calmodulin-dependent protein kinase.	EC:2.7.11.17	-	5.149e-27	-

Source	Gene names
Sma3	ATPK10; At1g01140; At1g29230; At1g30270; At2g25090; At2g26980; At2g34180; At4g18700; At4g30960; At5g01810; At5g01820; At5g07070; At5g10930; At5g21326; At5g25110; At5g35410; At5g45810; At5g45820; At5g58380; B1131G08.36; CIPK; CIPK1; CIPK10; CIPK11; CIPK12;

Source	GOs	Term	Type	Identity
Sma3	nucleus	GO:0005634	Cellular Component	-
Sma3	cytoplasm	GO:0005737	Cellular Component	-
Sma3	vacuole	GO:0005773	Cellular Component	-
Sma3	cytosol	GO:0005829	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	plant-type vacuole membrane	GO:0009705	Cellular Component	-
Sma3	protein serine/threonine kinase activity	GO:0004674	Molecular Function	-
Sma3	potassium channel activity	GO:0005267	Molecular Function	-
Sma3	protein binding	GO:0005515	Molecular Function	-
Sma3	ATP binding	GO:0005524	Molecular Function	-
Sma3	manganese ion binding	GO:0030145	Molecular Function	-
Sma3	potassium ion binding	GO:0030955	Molecular Function	-
Sma3	protein phosphorylation	GO:0006468	Biological Process	-
Sma3	signal transduction	GO:0007165	Biological Process	-
Sma3	response to nutrient	GO:0007584	Biological Process	-
Sma3	response to water deprivation	GO:0009414	Biological Process	-
Sma3	response to abiotic stimulus	GO:0009628	Biological Process	-
Sma3	response to salt stress	GO:0009651	Biological Process	-
Sma3	response to cytokinin stimulus	GO:0009735	Biological Process	-
Sma3	response to abscisic acid stimulus	GO:0009737	Biological Process	-
Sma3	abscisic acid mediated signaling pathway	GO:0009738	Biological Process	-
Sma3	negative regulation of abscisic acid mediated signaling pathway	GO:0009788	Biological Process	-
Sma3	potassium ion import	GO:0010107	Biological Process	-
Sma3	stomatal movement	GO:0010118	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	NAF domain	IPR004041	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	Ribosomal protein S14, conserved site	IPR018271	-	-
Sma3	NAF/FISL domain	IPR018451	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT5G45820.1	CIPK20, SnRK3.6, PKS18 CBL-interacting protein kinase 20 chr5:18587081-18588400 REVERSE LENGTH=439	61%
RefSeq	Arabidopsis thaliana	NP_199394.1	CBL-interacting serine/threonine-protein kinase 20 [Arabidopsis thaliana]	61%
RefSeq	Populus trichocarpa	XP_002323059.1	predicted protein, partial [Populus trichocarpa]	61%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9K4

Fln msg:

Fln protein: MEVKHNILMGRYELGRLLGQGTFAKVYYARSLKTGESVAIKAMDKEKILKVGMIEQIKREISVMRLVRHPNIVRLYEVMASKSKIYFVMEYVKGGELFNRVVENDILEEEEAKKYFQELISAVDFCHSRGVYHRDLKLENLLLDEKGVVKVSDFGLSALPAQFRPDGLLHTACGTPAYVAPEVITRKGYDGAKADIWSCGVILFVLLAGYLPFQGANLMVMYKKIYKGDFKCPTWFSWEARRLVLRLLDPDPQTRITIPQLMEVPWFRRGFKRPQGDTDISVGLLDDVDSAFSGPNPACLNAFDIISLSPGFNLSGLFKDDVLKQEKESRFTSTQPASSIMSKLEEIASLFRFSFKKSKEFKVKLKGSEQGRKGHLSIDAEIFEVTPSLFVVELRKTGGDTLEYSKFCSQDLRPGLKDIVLAWDGNRPEK*

Protein Length: 432

Fln nts: ATGGGACTTCACTTCTATAAAATGGGAGGTCCATGTGAACTCAGAGAGAAAACCCTTGTCTTCATGGTTTTATTTATTTTCCTGCATAAGAGCTTCATTAATCTTTTCTACCTTTAGAGAGCAACTCCATTAACCTGTTGTCTTTCTTTGCCTTCCTTACAGAACAACTCCATTACTTTTGAGTTGCAGCTGATATCGTCCTCTACCTTTTCCCACAAAAGCAATCTGTATATACAGAGAATGCTGTAAGATGCCCCTGCAGGACGCCCAGGCCATCAGCGGCCGCACTCAAGCACAGCTCGCGTGCAAAGAAAAGAGGCAGAAGATGAGCAGAAACACTCACGACGTCTGGAATGGAGTGCATATCGGAGAACAGGGCCTCGGCAGAGAAATCGCATAAACCCAACATATGCAAGAGAGCTAAGCAGCAGCAGGCGGATTTGTGACATTTTCTTTTGAAGTA_-_ATGGAGGTTAAGCACAATATTCTGATGGGGAGGTATGAATTAGGGCGCCTGTTGGGGCAAGGGACGTTCGCCAAGGTCTACTACGCCAGAAGTCTGAAGACAGGCGAGAGCGTGGCCATTAAAGCCATGGACAAGGAAAAAATCCTCAAGGTCGGCATGATCGAGCAGATTAAACGGGAAATCTCGGTTATGAGACTGGTTCGGCATCCCAACATCGTTCGGCTCTACGAAGTCATGGCGAGCAAGTCCAAGATTTACTTCGTCATGGAGTATGTTAAGGGCGGTGAGCTCTTCAACAGGGTTGTTGAAAATGATATACTGGAAGAGGAGGAGGCGAAGAAGTATTTTCAAGAATTGATTAGCGCGGTGGATTTCTGCCACAGCCGGGGTGTGTACCACCGGGATTTGAAGCTGGAGAACTTGTTATTAGACGAGAAAGGGGTCGTGAAGGTCTCGGACTTCGGCCTCAGCGCATTGCCGGCGCAATTCCGGCCGGACGGCCTTCTCCACACGGCCTGCGGCACGCCGGCTTATGTCGCACCGGAGGTCATTACCAGAAAGGGTTACGATGGCGCTAAAGCGGACATCTGGTCCTGCGGGGTTATACTGTTCGTGCTCCTGGCCGGCTACCTGCCGTTCCAGGGCGCGAATCTGATGGTGATGTATAAGAAGATCTACAAAGGGGACTTCAAGTGCCCGACCTGGTTTTCCTGGGAAGCACGGCGACTTGTCCTCCGGCTTCTGGACCCCGATCCGCAGACGAGGATCACCATTCCACAGCTGATGGAGGTCCCCTGGTTCCGCCGGGGTTTCAAACGCCCGCAAGGCGACACTGACATCAGTGTCGGACTCCTCGACGATGTCGACTCGGCCTTTTCCGGCCCGAATCCGGCCTGCCTGAACGCGTTCGACATAATCTCCCTCTCGCCGGGGTTCAATCTCTCCGGCCTATTCAAGGATGATGTCTTGAAGCAGGAGAAGGAATCGAGGTTCACATCGACTCAGCCGGCGTCAAGCATAATGTCGAAGCTGGAGGAGATTGCCAGTCTGTTTCGGTTCAGCTTTAAGAAATCGAAGGAATTCAAGGTGAAGCTGAAGGGATCGGAGCAGGGGCGGAAGGGGCATTTGTCGATCGATGCCGAGATTTTCGAAGTCACGCCCTCGCTGTTCGTGGTAGAGCTTCGGAAGACCGGCGGGGATACACTGGAATACAGTAAGTTCTGCAGCCAAGATCTGAGGCCCGGCCTTAAAGACATCGTGTTGGCATGGGACGGCAACAGACCAGAGAAGTAA___TAGCATATGTTCACCGTATATACGTCGTGATGGTTGCTCAGTTTTAAACAGTTATATTCACTGTATATACGTCGTGTTGGTTGTTTAGTTTTAAATGGTGTTTGTTTTACCAGACCGACGTTCAGTCCTCCTCGATCCAGAGAAAGCTCTCCTAATAGCATATATTCACTGTATATACGTGGTGATGGTTGCTTAGATTTAAATGGTTATGTTCACTGTATATACGTCGTGGTGTTTGCTTTGTTTTATAGGGTTTTCGTTTTACCAGACCGACGGCCAGGCCTCCTCGATCTAAAGAAAGCTCTATTCCGTACTTGGGCGGCGGTCCCCCTCCCGCGTCAATGTATGTATAAATACATCTGTCTCGATGTGAGCGGTTTTAATGGGTGAGAATGTGGATTTAATGTTATTTTATATTTTAAAGAAATAAA

Fln Alignment:

Contig10249___MEVKHNILMGRYELGRLLGQGTFAKVYYARSLKTGESVAIKAMDKEKILKVGMIEQIKREISVMRLVRHPNIVRLYEVMASKSKIYFVMEYVKGGELFNRVVENDILEEEEAKKYFQELISAVDFCHSRGVYHRXXXXXXXXXXXKGVVKVSDFGLSALPAQFRPDGLLHTACGTPAYVAPEVITRKGYDGAKADIWSCGVILFVLLAGYLPFQGANLMVMYKKIYKGDFKCPTWFSWEAXXXXXXXLDPDPQTRITIPQLMEVPWFRRGFKRPQGDTDISVGLLDDVDSAFSGPNPACLNAFDIISLSPGFNLSGLFKDDVLKQEKESRFTSTQPASSIMSKLEEIAXXXXXXXXXXXXXXXXXXXSEQGRKGHLSIDAEIFEVTPSLFVVELRKTGGDTLEYSKFCSQDLRPGLKDIVLAWDGNRPEK
D5A9K4_______________MEMKSNILMGRYELGRLLGQGTFAKVYYARNLKTGESVAIKVMAKEKILKVGMIEQIKREVSVMRLVRHPNIVRLYEVMASKTKIYFVMEYVKGGELFNRVADKGKLKEEEARKYFQQLISAVDFCHSRGVYHRDLKPENLLLDDNGVVKVSDFGLSALPEQFRQDGLLHTACGTPAYVAPEVITKKGYDGATADIWSCGVILFVLMAGYLPFQDANLMVMYKKIYKGDYKCPPWFSSEVRRLVLRLLDPNPRTRITVPQLMEVPWFRRGFKRPQADKDVNVGVLDDVDSAFFVPKPACLNAFDIISLSPGFNLSGLFKDDVLKQEEESRFTSTQPASSIMSKLEEIASLFRFSFKKSKDFKMKLQGSEQGRKGHLSIDAEIFEVTPSFFMVELRKTGGDTLEYSKFCSQDLRPGLKDIVWTWDGNRQEK

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
1958	87	12			0.992554
1960	87			12	0.992554
1962	87	12			0.992554
2048	90		10		0.90298
2059	90		10		0.90298
2095	83	16			0.994512
2096	16		83		0.994512
2097		83		16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain