SustainPineDB

Assembly Name: SustainPine v3.0

UniGene Name: sp_v3.0_unigene10156

Length: 906 nt

UniGene Fasta

Ace file of the UniGene sp_v3.0_unigene10156

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	pfam00759, Glyco_hydro_9, Glycosyl hydrolase family 9	-	-	0.0	63%
FL-Next	tr=Endo-beta-1,4-glucanase; Pinus radiata (Monterey pine) (Pinus insignis).	-	-	0.0	58%
Sma3	Endo-1,4-beta-glucanase	-	-	6.877e-36	-

Source	ECs	Term	Type	e value	Identity
Sma3	Cellulase.	EC:3.2.1.4	-	0.0	-

Source	KEGGs	Term	Type	e value	Identity
Sma3	Starch and sucrose metabolism	00500		0.0	%

Source	Gene names
Sma3	6J23.18; AT5G49720; Al-cel1; At1g02800; At1g19940; At1g22880; At1g23210; At1g48930; At1g64390; At1g70710; At1g71380; At1g75680; At2g32990; At2g44540; At2g44550; At2g44560; At2g44570; At3g43860; At4g02290; At4g09740; At4g11050; At4g23560; At4g38990; At4g39

Source	GOs	Term	Type	Identity
Sma3	extracellular region	GO:0005576	Cellular Component	-
Sma3	mitochondrion	GO:0005739	Cellular Component	-
Sma3	early endosome	GO:0005769	Cellular Component	-
Sma3	Golgi apparatus	GO:0005794	Cellular Component	-
Sma3	plasma membrane	GO:0005886	Cellular Component	-
Sma3	cell plate	GO:0009504	Cellular Component	-
Sma3	plant-type cell wall	GO:0009505	Cellular Component	-
Sma3	chloroplast	GO:0009507	Cellular Component	-
Sma3	integral to membrane	GO:0016021	Cellular Component	-
Sma3	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	-
Sma3	NADH dehydrogenase (ubiquinone) activity	GO:0008137	Molecular Function	-
Sma3	cellulase activity	GO:0008810	Molecular Function	-
Sma3	transferase activity	GO:0016740	Molecular Function	-
Sma3	carbohydrate binding	GO:0030246	Molecular Function	-
Sma3	carbohydrate metabolic process	GO:0005975	Biological Process	-
Sma3	cellular cell wall organization	GO:0007047	Biological Process	-
Sma3	pattern specification process	GO:0007389	Biological Process	-
Sma3	response to nematode	GO:0009624	Biological Process	-
Sma3	unidimensional cell growth	GO:0009826	Biological Process	-
Sma3	fruit ripening	GO:0009835	Biological Process	-
Sma3	cellulose biosynthetic process	GO:0030244	Biological Process	-
Sma3	cellulose catabolic process	GO:0030245	Biological Process	-
Sma3	hyperosmotic salinity response	GO:0042538	Biological Process	-
Sma3	cell wall modification involved in multidimensional cell growth	GO:0042547	Biological Process	-
Sma3	cortical microtubule organization	GO:0043622	Biological Process	-

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 9	IPR001701	-	-
Sma3	Six-hairpin glycosidase	IPR012341	-	-
Sma3	NADH:ubiquinone oxidoreductase, B12 subunit	IPR012576	-	-
Sma3	Glycoside hydrolase, family 9, active site	IPR018221	-	-

Source	Species	ID	Description	Identity
ATG	Arabidoptis thaliana	AT4G11050.1	AtGH9C3, GH9C3 glycosyl hydrolase 9C3 chr4:6747498-6751311 REVERSE LENGTH=626	73%
RefSeq	Arabidopsis thaliana	NP_192843.2	endoglucanase 19 [Arabidopsis thaliana]	73%
RefSeq	Populus trichocarpa	XP_002299478.1	predicted protein [Populus trichocarpa]	74%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: O64401

Fln msg: Distance to subject end: 196 aas, atg_distance in limit (1-15): atg_distance = 16, your sequence is shorter than subject: 292 - 510

Fln protein: MATLYSLLLLVLLSVGSAAAGHDYGEALSKSILFYEAQRSGKLPATQRANWRADSGLHDGFANGVDLIGGYYDAGDNVKFGLPMAYTITTLSWSVIEYGKQLANNGELGYAMEAIQWGTDYLLKAHREPNVLYGEVGDGNSDHYCWQRPEDMTTPRQAYKIDTSNPGSDLAGETAAAMAAASIVFKQSNPDYSNELLSHAKQLFEFADKYRGKYDSSITVVRKYYGSISGYADELLWAAAWLHEATEDRYYLNYMIDNAGTLGGTTWAMNEFSWDNKYAGVQVLASKFFMQD

Protein Length: 293

Fln nts: ATGGGGGTAATCCCATTCTGTATACAACA_-_ATGGCCACACTATATTCTCTGTTGTTGTTGGTTCTATTGTCAGTGGGGAGTGCTGCAGCAGGGCATGACTATGGTGAGGCCTTGAGTAAGAGCATTCTGTTTTATGAAGCTCAGAGATCTGGCAAGCTTCCTGCCACTCAGAGGGCCAATTGGAGAGCTGATTCTGGACTCCATGATGGATTTGCTAATGGGGTAGATTTGATTGGAGGGTACTACGATGCAGGTGACAATGTGAAATTCGGTCTTCCAATGGCATACACTATCACCACCCTCTCCTGGAGTGTGATTGAGTATGGAAAACAATTGGCTAACAATGGAGAATTGGGTTATGCCATGGAGGCCATTCAGTGGGGCACTGATTATCTTCTGAAAGCTCATCGTGAGCCAAATGTATTATATGGGGAGGTTGGTGATGGTAACTCAGACCATTACTGTTGGCAGAGGCCAGAAGATATGACAACACCAAGACAAGCCTACAAGATTGACACTAGCAATCCTGGATCAGATCTTGCAGGAGAAACAGCTGCTGCAATGGCAGCTGCTTCAATAGTCTTCAAACAATCTAATCCTGATTACTCTAATGAGCTTCTGAGCCATGCCAAACAGCTTTTTGAGTTTGCTGACAAGTACCGAGGAAAGTATGACAGCAGTATTACAGTCGTGAGAAAATATTATGGGTCAATTAGTGGATATGCTGATGAACTCCTGTGGGCAGCTGCATGGCTTCATGAAGCAACAGAAGACCGATACTACTTGAACTACATGATTGACAATGCAGGTACACTTGGTGGGACAACGTGGGCCATGAATGAATTCAGTTGGGATAATAAATATGCAGGGGTCCAAGTGCTCGCATCTAAGTTCTTCATGCAAGACA

Fln Alignment:

Contig10156___HDYGEALSKSILFYEAQRSGKLPATQRANWRADSGLHDGFANGVDLIGGYYDAGDNVKFGLPMAYTITTLSWSVIEYGKQLANNGELGYAMEAIQWGTDYLLKAHREPNVLYGEVGDGNSDHYCWQRPEDMTTPRQAYKIDTSNPGSDLAGETXXXXXXXSIVFKQSNPDYSNELLSHAKQLFEFADKYRGKYDSSI-TVVRKYYGSISGYADEXXXXXXXXHEATEDRYYLNYMIDNAGTLGGTTWAMNEFSWDNKYAGVQVLASKFFM
O64401_______________HNYRDALSKAILFYEGQRSGKLPGSQRMTWRRDSALSDGFSQHVDLVGGYYDAGDNVKFGFPMAFTTTMLSWSVLEFGGFMG--GELANSKDAIRWATDYLLKATAHPGTIYVQVGDPNTDHKCWERPEDMDTARTVYKIDSQHPGSDVAAETAAALAAASLVFRKSDPQYSKRLIHTAMRVFDFADKHRGAYSDSLRSCVCPFYCSYSGYEDELLWGAAWLHKATRNPTYLNY-IQSKGQSLGADESDNIFGWDNKHAGARVLLSKEFL

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
164	25	75			0.996473
167		75	25		0.996473
219		25		75	0.996473
271	20		80		0.995493
272	20		80		0.995493
274		80	20		0.995493
401	85	14			0.993533
404		85		14	0.993533
642	10			90	0.90298
643		10	90		0.90298
703		11		88	0.923564
706			88	11	0.923564
742	14		85		0.993533
746		85		14	0.993533
753	12		87		0.992554
754	12	87			0.992554
756			12	87	0.992554
757		87		12	0.992554
766	87			12	0.992554
767		87		12	0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain